Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F91 I1 R1 26 61.7 2321240 90.8% 2107685 142.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA98.3% 167.0 / NA 58P1100Q (CCG→CAG) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (31/26);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184403‑4184681
                                                                                                                                            |                                                                                                                                          
tCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGc                                                                                                                                          <  1:739478/143‑1 (MQ=255)
  tGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                        <  1:472466/143‑1 (MQ=255)
   ggCGGTTAAACGCCGTATTCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                       <  2:455832/143‑1 (MQ=255)
      ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGTCg                                                                                                                                    >  1:171895/1‑143 (MQ=255)
          aaaCGCCGTAGCCACCCTGGTGACAAGATGCCAGGTCGTCACGGCAACAATGGTGTAATCTCGAACATCCACCCGATCCAAGAAACGCCTTACAATTAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAcc                                                                                                                                <  1:1075058/143‑1 (MQ=255)
                cGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                          <  1:751522/143‑1 (MQ=255)
                 gTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGGACCGTCTCGt                                                                                                                         >  1:653614/1‑143 (MQ=255)
                   aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                       >  1:1068155/1‑143 (MQ=255)
                      cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                    >  2:150178/1‑143 (MQ=255)
                          cTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACAGTCTCGTATGAACATc                                                                                                                >  1:342621/1‑143 (MQ=255)
                           tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTCCTGAACCAGCTGGGCGTACGGTCCCGTAGGCACATCg                                                                                                               >  2:1150303/1‑143 (MQ=255)
                                aCAAGATGGCAGGTCGTCACGGTAACAAGGGAGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATGGGTAAg                                                                                                          >  1:12988/1‑143 (MQ=255)
                                 cAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGa                                                                                                         <  2:1068545/143‑1 (MQ=255)
                                     aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                     >  2:453701/1‑143 (MQ=255)
                                      tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                    >  1:885172/1‑143 (MQ=255)
                                        gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                  >  1:1070541/1‑143 (MQ=255)
                                        gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                  <  2:88581/143‑1 (MQ=255)
                                          agttcgtcACGGTAACAAGGGTGTAATCGCTAAGATCAACCCGATCGAAGATCGGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                <  1:512983/140‑1 (MQ=255)
                                          aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                <  1:453701/143‑1 (MQ=255)
                                          aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                <  1:922764/143‑1 (MQ=255)
                                                   ctgTAAGAAGGGGGTAATCGCTAAGATCAAACCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  2:405131/141‑1 (MQ=255)
                                                    ggTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCTGTAGAGGGCGTACTGAAGCAGCTGGGCGTACCGTCTCGTACGAACGTCGGCCAGATCCCGGAAACCCACCCggg                                                                                      >  2:873716/1‑143 (MQ=255)
                                                    ggTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTCTGAACATCGGTAAGATCCTCGAAACCCACCTggg                                                                                      >  2:589221/1‑143 (MQ=255)
                                                    ggTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTACGACCATCGGTCAGATCCTCGAAACCCACCTggg                                                                                      >  2:488558/1‑143 (MQ=255)
                                                         cAAGGGTGTAATTTCTAAGATCAACACGATCGAAGATATGCTTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATGGGTCAGATCCTTCAGACCCACCTGGGGATgg                                                                                 >  1:854494/1‑143 (MQ=255)
                                                          gaGGGTGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAGAACGGTACGCCTGTAGACATCGTACTGAACCAGCTGGGTGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                                <  2:692710/142‑1 (MQ=255)
                                                           gggggGTAATCTCGAAGGTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCt                                                                               <  1:1084252/142‑1 (MQ=255)
                                                               tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCCGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                           >  2:431156/1‑143 (MQ=255)
                                                                 tAATTTCGAAGCTCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGGACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  2:201264/143‑1 (MQ=255)
                                                                  aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTAGAAACCCACCTGGGTATGGCTGCGAAAg                                                                        <  1:425647/143‑1 (MQ=255)
                                                                   aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTGCCGTCTCGTATGGACATCGGACAGATCCTCGAGACCCACCGGGGGAGGGCGGCGAAAgg                                                                       >  2:730013/1‑143 (MQ=255)
                                                                      tCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTGCCGTCTCGTATGAACATCGGTCAGATCCGGGAAACCCACCTGGAGATGGCTGCGAAAGGTAt                                                                    >  1:587716/1‑143 (MQ=255)
                                                                         aaGACCGACCCGATCGACGCTCTGCCGTACGATGCAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCgg                                                                 <  2:481997/143‑1 (MQ=255)
                                                                          aGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACACGTTACTTAACCAGCGGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTGTGGCTGCGAAAGGTATCGGc                                                                >  2:822779/1‑143 (MQ=255)
                                                                            gtCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                              <  1:791019/142‑1 (MQ=255)
                                                                            aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                              >  2:911082/1‑143 (MQ=255)
                                                                              cAACCCGATCGAAGATATGCCTTACCATGAAAACGGTACGCCGGTAGACATCGTACTGATCCAGCTGGGGGTACCGTCTCGTATGAACATCGGTCAGATCCTCCAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACa                                                            <  2:141022/143‑1 (MQ=255)
                                                                                aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                          <  2:512295/143‑1 (MQ=255)
                                                                                        gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAACGGTCTCGGCGACAAGATCCACGc                                                  >  1:1015216/1‑143 (MQ=255)
                                                                                        gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACAGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGCTATGGCGGCGAAAGGTAGCGGCGACAAGAACAACGc                                                  >  2:400851/1‑143 (MQ=255)
                                                                                         aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATGGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCAGGGTATGGCTGCGAGAGGAATCGGCGCCAAGATCAACAcc                                                 >  2:839835/1‑143 (MQ=255)
                                                                                           gATATGCTTTACGATGTAAACGGTCCACCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAt                                               <  2:164429/143‑1 (MQ=255)
                                                                                                      cGATGAAAACGGCACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                    <  1:346951/143‑1 (MQ=255)
                                                                                                                ggTACGCCGGTAGATATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGATATCGGCGACAAGATCAACGCCATGCCGAAACAGCAGCaagaact                          >  2:92149/1‑141 (MQ=255)
                                                                                                                  tACGCCGGTAGACATCGTACTGACCCAGCTGGGCGTACCGGCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAGCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                        <  2:623516/143‑1 (MQ=255)
                                                                                                                  tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACGCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGACACAGCAGCAAGAAGTcg                        >  2:877945/1‑143 (MQ=255)
                                                                                                                         gTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCCTGCTGAAACAGCAGCAAGAAGTCGCGAAACt                 >  1:477408/1‑143 (MQ=255)
                                                                                                                           aGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc               <  1:888447/143‑1 (MQ=255)
                                                                                                                           aGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCATCGAAACCCACCAGGGGATGGCTGCGAAAGGTATCGGGGACAAGAAGAACGGAATGCCGAAAGAGACGCACGGAGGCGCGAAAcagg               >  2:1083257/1‑140 (MQ=255)
                                                                                                                            gACATCGGACTGAACCAGCCGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg              <  1:236528/143‑1 (MQ=255)
                                                                                                                             aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATACTCGAAACCCACCTGGGTCTGGCTGCGCAAGGTATCGGCGACAAGATCAACGCCCTGCTGAAGCAGCAGCACGAAGTCGCGAACCTgcgc             >  2:99366/1‑143 (MQ=255)
                                                                                                                                 cGTACTGAACCAGCTGGTCGTGCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt         <  2:653614/143‑1 (MQ=255)
                                                                                                                                   tACTGAGCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTG‑AACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa      <  2:15367/143‑1 (MQ=255)
                                                                                                                                   tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGCCAGGATCAACGCAACGCAGTCACGGTGGCAAGAAGTCGCGGAACTGCGCGAATTc       >  1:784123/1‑143 (MQ=255)
                                                                                                                                    aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTcc      >  1:461710/1‑142 (MQ=255)
                                                                                                                                       gAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCCGGTCCTCGAAACCCACCCGGGTATGCCTGCGAAAGGTATCGGCGACAAGATCAACGCCATACTGAAAAAGCAGCAAGAAGACGCGAAACTGCGCGAATTCATcc   >  1:794287/1‑143 (MQ=255)
                                                                                                                                        aaCCCGCTGCGCGTCCCGTCGCGTATGAACATCGGTCGGATCCTCGCAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCa  <  2:1068155/143‑1 (MQ=255)
                                                                                                                                        aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCAACCTGGGTATGGCTGCGAAAGGTATCGGCGATAGGTATAACGCCAGGCTGAAACAGCAGCAAGAAGTCACGAAAAAGCGCGAAATCAcccc  >  2:1003392/1‑142 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184403‑4184681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A