Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F104 I1 R1	25	31.6	1325236	90.2%	1195362	142.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,184,543	C→A	P1100Q (CCG→CAG)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,184,543	0	C	A	100.0%	106.1 / NA	36	P1100Q (CCG→CAG)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (22/14); total (22/14)

GGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4184409‑4184680

ggTTAAACGCCGTATCCAGCCTGATGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCAAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGCAGACAGCGCACTGAACCAGCTGGGCg                                                                                                                                   >  2:342123/1‑143 (MQ=255)

              tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCACCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                     <  2:403321/143‑1 (MQ=255)

                cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGACATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGaa                                                                                                                   >  2:358628/1‑143 (MQ=255)

                        tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                           >  1:554358/1‑143 (MQ=255)

                         gACAAGATGGCAGGTCGGCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCa                                                                                                          <  2:51587/143‑1 (MQ=255)

                                  gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAGCCAGCTGGGCGTACCGTCTCGCATGAACATGGGTCAGATCCTCGa                                                                                                 >  1:552850/1‑143 (MQ=255)

                                  gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                 >  1:624199/1‑143 (MQ=255)

                                    aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                               <  1:176253/143‑1 (MQ=255)

                                     ggTCGTCACGGTAACAAGGGTGTAAGTTCTAAGACCAACCCGAGCGAAGATATGCCTTACGATGAAAACGGTACTCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCTCGCAAGCACATCGGTCAGATCCTCGAAAc                                                                                              <  2:402605/143‑1 (MQ=255)

                                            aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTg                                                                                       >  1:567745/1‑143 (MQ=255)

                                                 aaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTCGACATCGTACTGAACCAGCTGGGCGTACCCTCGAGTACAACCATCGGGCCGAGCATCGAAGCCCGGCTGGGtgt                                                                                  >  2:422115/1‑141 (MQ=255)

                                                 aaCAAGGGCGTAATTTCTAAGATCAACCCGATCGAAGATATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGACCATCGGCCAGATCCCCGACACCCACCTGGGCAt                                                                                  >  2:97078/1‑143 (MQ=255)

                                                  aCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGCTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATg                                                                                 >  1:425958/1‑143 (MQ=255)

                                                  aCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgggggtg                                                                                 >  1:356702/1‑139 (MQ=255)

                                                       tgtgtAATTTCTAAGATCAACCCGATCGAAGATATCCCTTACGATGAAAACGGTACGCCGGTAGACTTCGTACTGATCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                            <  2:86181/142‑1 (MQ=255)

                                                          gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaa                                                                         <  1:488518/143‑1 (MQ=255)

                                                           tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAACAGCTGGGCGTACCGTCACGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGaaa                                                                        >  2:623844/1‑143 (MQ=255)

                                                               ttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTa                                                                    >  1:483135/1‑143 (MQ=255)

                                                                 ccaaGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCTGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc                                                                  <  1:14903/141‑1 (MQ=255)

                                                                  tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGACGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                 >  2:245446/1‑143 (MQ=255)

                                                                     gcggaaCCGGATCGAAGAGATGCCTTACGATGAGCGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGTGCGTACCGTCTGGTATGAACATCGGTCAGATCGTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCg                                                              <  2:501224/139‑1 (MQ=255)

                                                                        cAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAGAGGTCTCGGCGACa                                                           >  1:137804/1‑143 (MQ=255)

                                                                         aaCCCGATCGAAGATATGCCTGACGCTGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                          <  2:617835/143‑1 (MQ=255)

                                                                          aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGGAAGGTGTCgggggcggg                                                         >  1:132778/1‑136 (MQ=255)

                                                                            ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCACGTATGAACATCGGTCAGATCCTCGAAGCCGACCTGGGTATCGCTGCGAAAGGTATCGGCGACAAGac                                                       >  2:167004/1‑142 (MQ=255)

                                                                                    ggATATGCCTTACGATGAAAACGGTACGCCGGTAGACACCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa                                               <  1:131177/142‑1 (MQ=255)

                                                                                               aCGATGAAAACGGTCCGCCGAGAGACATCGCACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGCGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                    <  1:320474/143‑1 (MQ=255)

                                                                                                cGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                   >  1:426374/1‑143 (MQ=255)

                                                                                                 gacgAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                  <  1:130988/140‑1 (MQ=255)

                                                                                                 gATGAAAACGGTAGGCCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACGTCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                  <  1:484047/143‑1 (MQ=255)

                                                                                                    gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGCTCAACGCCATGCTGAAACAgcagca                               >  1:81666/1‑143 (MQ=255)

                                                                                                    gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCAACAAGATCAACGCCATGCTGAAACAgaagca                               >  2:276890/1‑143 (MQ=255)

                                                                                                    gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCAGGGGATGGCTGCGAAAGGTATCGGCGACAAGAAGAACGAGATGAGCAAACAgcagca                               >  2:133680/1‑143 (MQ=255)

                                                                                                      aaaCGGTGCGACGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                             <  1:50403/143‑1 (MQ=255)

                                                                                                       aaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCCCGCAACCCACCTGGGTATGGCCGCGAAAGGTCTCCGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                            >  2:583285/1‑143 (MQ=255)

                                                                                                                                 gAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAACGGCGACAAGATCAACGCCAAGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATcc  >  1:557943/1‑143 (MQ=255)

GGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4184409‑4184680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A