Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F91 I0 R1
|
49 |
33.8 |
1296440 |
89.8% |
1164203 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,184,543 |
C→A |
100% |
P1100Q (CCG→CAG) |
rpoB → |
RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,184,543 | 0 | C | A | 100.0%
| 103.6
/ NA
| 34 | P1100Q (CCG→CAG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/20); total (14/20) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCG > NC_000913/4184405‑4184671
|
tGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg > 2:412517/1‑143 (MQ=255)
cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACCCCGGTCGACAACGTACTGAACCAGCTGGGc > 2:206708/1‑143 (MQ=255)
ccGTATCCAGCCTGGTGACGAGATGGCAGGTCGTCACGGTAATAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc < 2:202503/143‑1 (MQ=255)
gTAGCCAGCCGGGTGACACGATGGCGGGTCGTCACGGTAAGAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt < 2:620884/143‑1 (MQ=255)
tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg > 1:613513/1‑143 (MQ=255)
ccggCCTCGTGACAAGACGGCAGGCCGTCACGGTAACAAGGGTGTAATTTCGAAGATCAACCCGATCGAAGCTATGCCCTACAATGAAAACGCTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa < 2:107042/140‑1 (MQ=255)
ccAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa < 1:38850/143‑1 (MQ=255)
cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa > 1:557382/1‑143 (MQ=255)
aGCCTGGTAACATGGTGGCAGGTCTGCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAc < 2:22736/143‑1 (MQ=255)
aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAACATCAACCCGATCGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACAGCGTACTGCACCAGTTGGGCTCACCTACTCGTATGACGAGCTGTCAGATCCt < 2:462055/143‑1 (MQ=255)
aGGTCATCACGGTAACAAGAGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa < 1:254408/143‑1 (MQ=255)
gcgggaCAAGGCTGTAAGTTCTAAGCGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTATACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg < 2:587458/138‑1 (MQ=255)
caaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTa < 2:375876/142‑1 (MQ=255)
aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGc > 1:421439/1‑143 (MQ=255)
gggTGTAATTTCCAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTg < 2:507483/143‑1 (MQ=255)
tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAAGAACATCGGCCGGAGCCGCGAACCCCACCGGGACAG‑GGCTggca > 2:570269/1‑140 (MQ=255)
tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGaaa > 1:459322/1‑143 (MQ=255)
tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGCTCCTCGCAACCCACCTGGGTAT‑GGCTGCGAAAGGTAGCg > 2:149784/1‑143 (MQ=255)
aaGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATGGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCCTCGAAACACACCAGGGTAT‑GGCTCCGAAAGGTAACgg > 2:124220/1‑143 (MQ=255)
gATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTAgcggcg > 2:533402/1‑143 (MQ=255)
aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGCCATCGTACTGAACCAGCTGGGCGTACCGCCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCCGCGCAC‑GACTGCGAAACGCAGCGGCGa > 2:331821/1‑143 (MQ=255)
tCGAAGCTATGCCCTACGCTGAAACCGGTACGCCGGGAGACATCGTACTGAACCAGCTGTGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAAc < 2:614251/143‑1 (MQ=255)
gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGc < 2:609259/143‑1 (MQ=255)
aaGATATGCCTTACGATGAAAACGGGACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGcc < 1:545902/143‑1 (MQ=255)
gtatGCCTTACGATGGTGCCGGTACGCGGGTAGACATCGTACTGCACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg < 2:612572/142‑1 (MQ=255)
gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGa < 1:539147/143‑1 (MQ=255)
ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGGATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGGAAGGTATAGGGGACGAGATCAACGCCATGCTGaacc > 2:30842/1‑141 (MQ=255)
tGAAAACGGTACGCCGGTAGACCGCGTACTGCACCAGCTAGTCTTACCGTCTCGTATGCACATACGTAATAATCTCGACGGTCTTC‑GGGTCTACGCCGCGAAAGTTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc < 1:299033/143‑1 (MQ=255)
agAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc < 1:298170/142‑1 (MQ=255)
gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACCgcagca > 1:193853/1‑143 (MQ=255)
gaCGCGGGAAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg < 2:441038/142‑1 (MQ=255)
aGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc < 1:97119/143‑1 (MQ=255)
aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc < 2:17297/143‑1 (MQ=255)
cATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTGT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCCGCAAGAAGTCGCGACACTGcgcg > 1:498187/1‑143 (MQ=255)
|
TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAT‑GGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCG > NC_000913/4184405‑4184671
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
