Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F112 I1 R1 26 41.0 1730060 90.4% 1563974 142.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA94.3% 100.5 / NA 36P1100Q (CCG→CAG) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (11/22);  total (12/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC  >  NC_000913/4184406‑4184679
                                                                                                                                         |                                                                                                                                        
ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                     <  1:409262/143‑1 (MQ=255)
 gCGGTTAACCGCCGTATCCAGCCGGGTGACAAGATGGCAGGTTGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTggg                                                                                                                                    <  2:540408/143‑1 (MQ=255)
 gCGGTCAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGCTCACAAGCGTGTAATTGCTAAGACCAACCCGATCGACGATATGCCTTACGCTGAAAACGCTACGCCGGTCCCCATCGTACTGACCCCGCCggg                                                                                                                                    >  2:366584/1‑143 (MQ=255)
      tggaCGCCGTAGCCAGCCTGGTGACGAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCGAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                               <  2:716699/140‑1 (MQ=255)
            ccGTATCCAGCCTGGTGACACGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                         <  1:67150/143‑1 (MQ=255)
            ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                         >  1:293281/1‑143 (MQ=255)
               tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                      <  2:711239/143‑1 (MQ=255)
                   cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                  <  2:446148/143‑1 (MQ=255)
                          gggACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                           <  2:97399/141‑1 (MQ=255)
                          gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                           <  1:31961/143‑1 (MQ=255)
                               aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                      >  2:393995/1‑143 (MQ=255)
                                     gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAACGTCTCGGATGAACAACGGTCAGATcccaga                                                                                                >  2:1344/1‑139 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACACCGGTCAGATCCTCGaca                                                                                              >  1:520628/1‑141 (MQ=255)
                                          tcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaaaac                                                                                           >  1:109591/1‑140 (MQ=255)
                                           cgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCa                                                                                          <  1:170334/143‑1 (MQ=255)
                                                 ggTAACAGGGGTGTAATGTCTAAGAGCGACCCGATGGGAGATATGCGTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTggg                                                                                    <  2:260233/143‑1 (MQ=255)
                                                    aaCAAGGGTGTAATTTCTAAGATCACCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                                 <  1:268905/143‑1 (MQ=255)
                                                          ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATGCTCGAAACCCACCCGGGTATGGCTGc                                                                           >  2:246140/1‑143 (MQ=255)
                                                           gtgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCATCGAAACCCACCTGGGTATGGCTGCg                                                                          >  1:709631/1‑143 (MQ=255)
                                                            tgtAATTTCTAAGATCAACCCGATCGAAGATATGGCTTACGATGAAAACAGTACGCCGGTAGAACTCGTACATAACCAGCTTGGTCTACCGTCTCTTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                         <  1:398511/143‑1 (MQ=255)
                                                            tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGa                                                                         >  1:754339/1‑143 (MQ=255)
                                                               aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                      <  2:422845/143‑1 (MQ=255)
                                                                    cTAAGCTCAACCCGATCGAAGATATGCCTTACGATGAACACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc                                                                 <  2:544446/143‑1 (MQ=255)
                                                                         aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAACGTGGATGAGCTTCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                            >  2:605755/1‑143 (MQ=255)
                                                                              cccGATAGAGCATATGCACTATGATGAAAACGGGGCGCCGGCAGTCATCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                       <  2:247703/143‑1 (MQ=255)
                                                                               cctaTCGAAGATATGCCTTGCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAGCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGAt                                                      <  2:509441/140‑1 (MQ=255)
                                                                                    cGAAGATAGGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACg                                                 <  1:806136/143‑1 (MQ=255)
                                                                                               cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGATCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCCGCGACAAGATCAACGCCATGCTGaaa                                      <  2:823143/143‑1 (MQ=255)
                                                                                                ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCGGAGAAAGGGATCGGCGACAGGCTCAACGCCATGCTGAAAc                                     >  1:795476/1‑143 (MQ=255)
                                                                                                        gacaCGGTACGCAGGTAGACATGGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                             <  1:728845/140‑1 (MQ=255)
                                                                                                            cgctACGCCGGTAGACATCGTACTCATCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                         <  1:248722/140‑1 (MQ=255)
                                                                                                             gggccGCCTGTGGGCAGCGTACTGACCCGGTTCGGCGTTCCGTCGCGTATGAACATCGGTCGGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGt                        <  2:369877/139‑1 (MQ=255)
                                                                                                                aCGCAGGTAGACATCGTACTGATGTAGCTGGGCGTACCGTCTCGTATGAACATCTGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                     <  2:724617/143‑1 (MQ=255)
                                                                                                                         gACATCGTACTGCACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg            >  1:399558/1‑143 (MQ=255)
                                                                                                                             ccctACCGAAGCAGCCGGGCGTCCCGTCTCGTATGACCAGCGGTCCGATCCTCGAAACCCCCCTGGGTACTGCTTCGAAAGGTCTCGGACCCCAGTTCAACGCCATGCTGAAACAGCAGCCAGAAGTCGCGAACCTGCCCGac        <  2:594684/140‑2 (MQ=255)
                                                                                                                                   tGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc  <  1:393995/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC  >  NC_000913/4184406‑4184679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A