Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F87 I0 R1
|
87 |
48.8 |
1893308 |
89.3% |
1690724 |
142.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,184,543 |
C→A |
100% |
P1100Q (CCG→CAG) |
rpoB → |
RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,184,543 | 0 | C | A | 100.0%
| 146.3
/ ‑6.7
| 52 | P1100Q (CCG→CAG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (29/22); minor base T (0/1); total (29/23) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATT > NC_000913/4184407‑4184675
|
gCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTggg > 1:16010/1‑143 (MQ=255)
gCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCAAGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACCGAACCAGCTggg > 2:687294/1‑143 (MQ=255)
tAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc < 1:205676/143‑1 (MQ=255)
aaggCCGTATCCAGCCTGGTTACAAGATGGCAGGTCGTACCGATCAACTACTGT‑TTATTTCTAAGATCAACCCGATCGAAGATATGCCTTACTATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCg < 1:735571/140‑1 (MQ=255)
gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGAAGACATCGTACTGAACCAGCGGGGCGTACCGtac > 2:521415/1‑141 (MQ=255)
ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGGACCGtctc > 2:830847/1‑143 (MQ=255)
tCCAGCCTGGTGCCAAGATGGCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAATCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg < 1:281459/143‑1 (MQ=255)
ccTGTTGACACGATGTCACGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGACCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAt < 2:745658/143‑1 (MQ=255)
tGACAAGATGGCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc > 2:644921/1‑143 (MQ=255)
caggATGGCAGGTCGTCACGGT‑AACAAGGGTGTAATGTCTCAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATAAACATCGGTCAGa < 2:395213/140‑1 (MQ=255)
gATGGCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATcc > 2:79533/1‑143 (MQ=255)
aTGGCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAGACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGAACATCGGTCAGATCCt < 2:511833/143‑1 (MQ=255)
ttgCATGTCGTGAGGTT‑AATAAGGGTGTAATTTCTAATATCAAGCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc < 1:777487/141‑1 (MQ=255)
gCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa < 1:560194/143‑1 (MQ=255)
aggaccccACGGT‑AACAAGGGTGGAATTTCTAAGAGCAACCCAATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACGTCGTACTGAACCAGCTGGGAGCACCGCATCGAAAGGACATCGGTCAGCTAGTCGaaa > 2:338732/8‑143 (MQ=255)
aGGTCGTCACGGT‑AACATGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa < 1:653284/143‑1 (MQ=255)
aGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGATCAGATCCTCGaaa > 2:383408/1‑143 (MQ=255)
aGGGCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCGACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa < 2:398942/143‑1 (MQ=255)
tcACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAcc > 1:463875/1‑143 (MQ=255)
aCGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTg > 1:859303/1‑143 (MQ=255)
ggT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTggg < 1:493906/143‑1 (MQ=255)
cgctgagaaGGGTGTCATTTCTACGAGCATCCCCATCGATGTTACTCCTTACGGCGACCACGGTACGCCGCTAGACATCGTACTCACCCTGCGTGCCCTACCCTCTCGTATGATCATCGGTCAGATCCTCGAAACCCACCTgg < 2:847034/136‑1 (MQ=255)
aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAACCCGCCTGGGGATGGc > 2:86795/1‑143 (MQ=255)
ggTGTATTTTCTAGCATCAACCCGATCGTACATATGTCTTAGGATGGAAACGCTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAATCCCACCTGGGTATGGCTGc < 2:722626/143‑1 (MQ=255)
cgtgtAATCTCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc < 1:864131/142‑1 (MQ=255)
tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGATCAGATCCTCGCAACCCACCTGGCTCCGGCTGCgc > 2:207934/1‑142 (MQ=255)
tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGATCCTCGAAACCCACCCGGGTATGGCTGCGaaa > 2:425953/1‑143 (MQ=255)
tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGCGTCTGGCTGCGaca > 1:100551/1‑141 (MQ=255)
caaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa < 1:540758/142‑1 (MQ=255)
aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTCTGGCTGCGACAg > 1:279393/1‑143 (MQ=255)
ttgcTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTATTCATCGTATTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGt < 1:572371/140‑1 (MQ=255)
cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc > 1:707567/1‑143 (MQ=255)
tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTCGACATCGTACTGGACCAGCGGGGCGTACCGTCGCGTAGGAACGTCGGTCAGACCCGCGAAACCCACCTGGGCATGGCTGCGAAAGGTAACg > 2:43844/1‑143 (MQ=255)
aGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCggg > 1:883422/1‑142 (MQ=255)
aGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGc > 2:838508/1‑143 (MQ=255)
gATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGCATCGGCg > 1:753450/1‑143 (MQ=255)
agcccactCGAAGATATGCCTTCCGGTGCAAGCGGTCCGCCGGTAGACCTCGTACTGAACCACCTGGGCGTACCGCCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa < 2:308502/136‑1 (MQ=255)
tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGGGTAGCGTGTGGTATGAACATCGGTCAGATCCTCCAACCCCACCTGGGTATGGCTGCGAAACGTATCGGCGACCAGACCaag > 2:871748/1‑142 (MQ=255)
tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAACCTCGAAACCCACCTGGGTATGGCTGCGAAAGCGCTCGGCAACAAGAGCacc > 2:868402/1‑141 (MQ=255)
aaGAGATGCCTTAGGAGGAAAACGGTACGCCGGTAGACAGCGGACTGAACGAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc < 1:57621/143‑1 (MQ=255)
cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa < 2:707567/143‑1 (MQ=255)
gatgatAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca < 1:424868/143‑1 (MQ=255)
aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag > 1:665829/1‑143 (MQ=255)
tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc < 1:79533/143‑1 (MQ=255)
gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCGGAAACAgcagca > 1:484999/1‑143 (MQ=255)
aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATAGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGGGAGGTATCGGCGACAAGATCAACGCCATGCGGCAACAGGGGCaa > 1:101853/1‑143 (MQ=255)
aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag > 1:918021/1‑143 (MQ=255)
ccGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaa > 1:881460/1‑143 (MQ=255)
ggTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc < 1:796299/143‑1 (MQ=255)
aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGa > 1:804435/1‑143 (MQ=255)
tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCCGCAACAGCAGCAAGAAGTCCCGCACCCgcgcaga > 2:553599/1‑140 (MQ=255)
gTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAtt < 2:209587/143‑1 (MQ=255)
|
GCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGT‑AACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATT > NC_000913/4184407‑4184675
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
