Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F61 I1 R1 22 65.1 2541758 91.4% 2323166 141.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA98.4% 196.6 / NA 65P1100Q (CCG→CAG) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (31/32);  minor base G (1/0);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC  >  NC_000913/4184410‑4184679
                                                                                                                                     |                                                                                                                                        
gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGt                                                                                                                                 >  1:683355/1‑143 (MQ=255)
      cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGCTCGTACCGtc                                                                                                                           >  1:463811/1‑143 (MQ=255)
       gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTCCCGtct                                                                                                                          >  1:575598/1‑143 (MQ=255)
        ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGCCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                         <  2:1033466/143‑1 (MQ=255)
         cGTAGCCAGCCTGGTGACAAGATGGCAGGTCGGTACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                        <  1:1152432/143‑1 (MQ=255)
             tCCAGACTGGTGACAAGCTGGCAGGTAGTCACGCTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAGAACGGTACGCCTGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                    <  2:789882/143‑1 (MQ=255)
               cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTAGGCCGGGAGACAGCGTACTGCACCGGCTGGGCGTACCGCCCCGTATGac                                                                                                                  >  1:1255645/1‑142 (MQ=255)
               cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                  >  2:737863/1‑143 (MQ=255)
               cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATgga                                                                                                                  >  1:236923/1‑141 (MQ=255)
                    tGGCGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATCTGCCTTCCGATGAAAACGGTACGCCGGTAGACATAGCACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAGCg                                                                                                             >  2:320218/1‑143 (MQ=255)
                      gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                           >  2:874522/1‑143 (MQ=255)
                           gaggtGGCAGGTCGGCACGGTAACAAGGGTGTAAGGTCGAAGAGCAACCCGATCGAAGATATGCCGTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                      <  2:441509/139‑1 (MQ=255)
                           cagcgggCAGTTCGTCACGGTAACACGGGTGTAATTTCGAAGAGCAACCCGATCGAAGATATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                      <  2:778964/138‑1 (MQ=255)
                           aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                      >  1:64237/1‑143 (MQ=255)
                           aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                      >  1:614923/1‑143 (MQ=255)
                                 gCAGGTCGTCACGGTAACAAGGGTGTAAGTTCCAAGATCAACCCGATCGAAGATATGCCTTACGGTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                <  2:1057298/143‑1 (MQ=255)
                                   agttcgtcACGGTAACAAGGGGGTAATTTCTACGATCAACCAGATTGAAGTTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                              <  2:12389/140‑1 (MQ=255)
                                   aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                              <  2:683355/143‑1 (MQ=255)
                                   aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTACGCACATCGGTCAGATCCTCGaaa                                                                                              >  2:798358/1‑143 (MQ=255)
                                    ggTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAc                                                                                             >  1:806495/1‑143 (MQ=255)
                                          cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCAACCt                                                                                       <  1:311345/143‑1 (MQ=255)
                                           aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCTGATCCTCGAAAGCCACCTg                                                                                      >  1:882028/1‑143 (MQ=255)
                                           aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTg                                                                                      <  1:53795/143‑1 (MQ=255)
                                           aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTAGAAGCACACCTg                                                                                      >  1:885979/1‑143 (MQ=255)
                                            cGGCAACAAGGGTGTAATTTCTACGATCAACCCGATCGAAGATATGCCTTACAAAAATCATATTATGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                     <  1:290285/143‑1 (MQ=255)
                                                 aCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGCAACCCCCCTGGGTATg                                                                                >  1:426954/1‑143 (MQ=255)
                                                   aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATCGGGCAGATCCTCGAAACCCACCCGGGTATgcc                                                                              >  2:624723/1‑141 (MQ=255)
                                                    aGGGTGTAATTTCTAAGATCAGCCCGAGCGAAGATATGCCTTACGAGGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCt                                                                             <  2:469205/143‑1 (MQ=255)
                                                    aGGGGGTAATTTCTAAGACCAACCCGATTGAAGATATCCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCt                                                                             <  2:1025020/143‑1 (MQ=255)
                                                        tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                         >  2:641738/1‑143 (MQ=255)
                                                        tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                         >  1:391936/1‑143 (MQ=255)
                                                          tAATTTCTAAGATCAACCCGATCGAAGCTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGCACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                       <  1:326716/143‑1 (MQ=255)
                                                           tatgtCTAATATCAACCCGATCGAAGATATGGCGTACGACGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                      <  1:126907/139‑1 (MQ=255)
                                                           aagttCTAAGATCAACCCGATCGAGGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                      <  2:1202447/140‑1 (MQ=255)
                                                               tCTAAGATCAACCCGATCGAAGATATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAt                                                                  <  1:888014/143‑1 (MQ=255)
                                                                cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGCTATc                                                                 >  1:309719/1‑143 (MQ=255)
                                                                   aGATCAACGCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGc                                                              <  1:168667/143‑1 (MQ=255)
                                                                    gATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCAAAACCCACCTGGATATGTCTGCGAAAGGAATCggga                                                             >  2:114161/1‑141 (MQ=255)
                                                                     aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                            >  2:289768/1‑143 (MQ=255)
                                                                     aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                            >  1:465673/1‑143 (MQ=255)
                                                                      tccatccGATCGAATATATGTCTTAGGATGGTAACGTGACGCTGGTATACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGTTGCGAAAGGTATCGGCGAc                                                           <  2:68815/138‑1 (MQ=255)
                                                                          cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATTGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                       >  2:912389/1‑143 (MQ=255)
                                                                           ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                      <  1:878614/143‑1 (MQ=255)
                                                                               tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                  >  1:775625/1‑143 (MQ=255)
                                                                               tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAACCCACCTGGATATGGCTGCTAAAGGTATCGGCGACAAGATCAAc                                                  <  1:803804/143‑1 (MQ=255)
                                                                                        tGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCATTTGTACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTg                                         <  1:293695/143‑1 (MQ=255)
                                                                                            ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                     >  1:1036337/1‑143 (MQ=255)
                                                                                            tggggATGAAAGCGGGAGGCCGGTAGACAGCTGAGTGGCCCATCGGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTCGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                     <  2:326902/139‑1 (MQ=255)
                                                                                                 aTGAAAACGGTACGCCGGTAGACATTGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                <  1:912389/143‑1 (MQ=255)
                                                                                                 aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCCACGCCATGCTGAAAcagcag                                >  1:71944/1‑143 (MQ=255)
                                                                                                    aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagcga                             >  2:997390/1‑141 (MQ=255)
                                                                                                    aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                             <  1:1158624/143‑1 (MQ=255)
                                                                                                          gTACGCCGGCAGACATACACCGCAACCAGCTCCGCGTACCGCCTCGGATGAACATCGGTCAGCTCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                       <  1:171756/143‑1 (MQ=255)
                                                                                                            aCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                     <  1:448990/143‑1 (MQ=255)
                                                                                                               ccGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAAATAAAAGCCATGCTGAACAGGCAGCAGGAAGGCGCGag                  >  2:789575/1‑142 (MQ=255)
                                                                                                                cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaaa                 <  1:737863/143‑1 (MQ=255)
                                                                                                                      aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCAATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc           >  1:247450/1‑143 (MQ=255)
                                                                                                                      aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACATGGGTATGGCTGCGTAAGGTATCGGCGACAAGATCAACGCCATGCTAAAACAGCCGCAAGACATCGCGGGACTgctc           >  1:229071/1‑141 (MQ=255)
                                                                                                                        aTCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGa         <  2:984898/143‑1 (MQ=255)
                                                                                                                           gTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGAGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAtt      >  1:720915/1‑143 (MQ=255)
                                                                                                                           gTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGGCAGAGCCTCGAAACCCACCTGGGTATGGCCGGGAAAGGAAGCGGCGCCACGATCAACGCCATGCCGCAAAAGCAGCCAGAAGTAGCGAAAGTACGCGACtt      >  2:812334/1‑143 (MQ=255)
                                                                                                                             aCTGAACCTGCTGGGTGTACCGTCTCGTATGACCACCGGTCAGAGCCTCGAACCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa    <  2:1195551/143‑1 (MQ=255)
                                                                                                                             aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa    <  2:64237/143‑1 (MQ=255)
                                                                                                                             aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa    <  2:614923/143‑1 (MQ=255)
                                                                                                                               tgtaCCAGCTGGGCCTTCCGTCTCGTATGGACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCGGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc  <  2:327060/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
GTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC  >  NC_000913/4184410‑4184679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A