Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F21 I0 R1 50 53.6 2034412 90.9% 1849280 142.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA100.0% 209.9 / NA 68P1100Q (CCG→CAG) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/33);  total (35/33)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4184406‑4184680
                                                                                                                                         |                                                                                                                                         
ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                      <  1:800292/143‑1 (MQ=255)
ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                      >  1:810506/1‑143 (MQ=255)
 gCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTggg                                                                                                                                     >  1:27993/1‑143 (MQ=255)
   ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCg                                                                                                                                   >  1:346898/1‑143 (MQ=255)
   ggTTAAACGCCGTATCCAGACTGGAGACAAGATGGCAGGTCGTCACGGTAACAAGGGAGTAATTTCTAATATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGACGGTAGACATCGTACTGAACAAGCTGTGca                                                                                                                                   >  2:307130/1‑142 (MQ=255)
         aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAACCGGTACGCCGGTAGACATCGTCCTGACCCAGCTGGGCGTACCGt                                                                                                                             >  2:734919/1‑143 (MQ=255)
         aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                             >  1:341685/1‑143 (MQ=255)
            ccGTATCCAGCCTGGTGACAAGTTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                          <  1:387211/143‑1 (MQ=255)
             cGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                         <  1:198529/143‑1 (MQ=255)
               tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGGTGGGCGTACCGTCTCGTa                                                                                                                       >  2:20273/1‑143 (MQ=255)
                agccAGCCTGGGGACAAGAGGGCAGGTCGTGACGGTAACAAGGGTGGAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGTGTACCGTCTCGTAt                                                                                                                      <  2:993117/141‑1 (MQ=255)
                 tCCAGGCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                     <  1:316888/143‑1 (MQ=255)
                    aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACAGTACGCCGGTAGACATCGTACTGAACCAGCTGGGAGTCCGGTCTCGTATGAAc                                                                                                                  >  2:123291/1‑143 (MQ=255)
                    aGCCTGGTGACAAGATGGCAGGTAGTCACGGTAACAAGGGTGTAATTTCTAAGATCAAACGGATAGAAGAAAAGCCTTAAGATGAAAAAGGTCCGACGGGAGACAGCGTAATGAACCAGAGGGGCGGAGCGTATGGTA‑GAACa                                                                                                                 >  2:849955/1‑143 (MQ=255)
                            gACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCa                                                                                                          >  1:433212/1‑143 (MQ=255)
                              cAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCCCGTCTGCACACCAGTCaca                                                                                                        >  1:964282/1‑141 (MQ=255)
                               aatatcgCAGGTCGTCACTTCAACATTGGTGTAATTTCTAAGATCAACCCGACCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTTGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                       <  1:304727/137‑1 (MQ=255)
                               aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCCGAt                                                                                                       >  1:963878/1‑143 (MQ=255)
                               aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCTCGTATGAACATCGGTCAGAt                                                                                                       >  2:190571/1‑143 (MQ=255)
                               aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCGGGGCGTACCGACTCGTATGAAAAGCGGCCAGAt                                                                                                       >  2:271514/1‑143 (MQ=255)
                                 gATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATcc                                                                                                     >  1:639976/1‑143 (MQ=255)
                                   tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGGTGAAAACGGTACGCCGGTAGACATCGGACTGAACCAGCGGGGCGTACCGCCTCGGATGAGCAGCGGTCAGATCCtg                                                                                                   >  2:119295/1‑142 (MQ=255)
                                       agggccgcACGGGAAGAAGGGTGTAATGTGTGAGATCCCCCCGGTNCAAGATATGCCTTACGATGAAAGCGGTACNCCGGTAGACATCGTACTGNCCCAGCNGGGCGTNCCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                               <  2:735684/136‑1 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCATCCCGATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATAAACATCGGTCAGATCCTCGaaa                                                                                               <  2:942834/143‑1 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                               <  2:502070/143‑1 (MQ=255)
                                       aGGTCGTCACGAGAAGAAGGGTGTAATTTCTAAGAGCAACGCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                               <  1:636074/143‑1 (MQ=255)
                                             tgccGGTAGCAAGGCGGTAGTTTCTTAGTCCACCCCGATCGAAGATTTGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAcc                                                                                         <  2:486934/140‑1 (MQ=255)
                                              cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                        <  1:383806/143‑1 (MQ=255)
                                                 ggTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTggg                                                                                     >  2:475948/1‑143 (MQ=255)
                                                      cAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                                <  2:179405/143‑1 (MQ=255)
                                                      cAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGACAGATCCTCGAAACCCACCTggggaggg                                                                                >  2:686475/1‑138 (MQ=255)
                                                          ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAGCCAGCTGGGCGTACCGCGTCGTCNGATCACCGGTCAGATCCTCGAACCCCACCTGGGTATGGCTGc                                                                            >  2:350111/1‑143 (MQ=255)
                                                            tgtAATTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                          <  1:706222/143‑1 (MQ=255)
                                                             gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaa                                                                         <  1:674361/143‑1 (MQ=255)
                                                              tagtttCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                        <  1:655646/140‑1 (MQ=255)
                                                              tAATTTCGAGGAGCGAGCCGATCGAAGATAGGCCCTACGATGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                        <  2:320669/143‑1 (MQ=255)
                                                               aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                       <  1:494978/143‑1 (MQ=255)
                                                                  ttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGCACCAGCCGGGCGGACCGTCTCGTAAGAGTGTCGGTCAGATCCTCGAAACCCACCTCGGTATGGCTGCGAAAGCTa                                                                    >  1:38277/1‑143 (MQ=255)
                                                                    cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTGTc                                                                  >  1:374460/1‑143 (MQ=255)
                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                 <  1:562181/143‑1 (MQ=255)
                                                                       ggATCACCCCGCTCGACGAGATGCCTTACGATGAAAACGGGACGCCGGTAGACATCGTACTGACCCAGCTGGACGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGc                                                               <  2:484349/142‑1 (MQ=255)
                                                                       aGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGc                                                               >  2:428539/1‑143 (MQ=255)
                                                                         aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGGACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                             <  2:27993/143‑1 (MQ=255)
                                                                            aaCCCGATCGACGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                          <  1:329493/143‑1 (MQ=255)
                                                                            aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                          >  1:901829/1‑143 (MQ=255)
                                                                              cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAGAGGGATCGGCGCCAAGa                                                        >  1:419119/1‑143 (MQ=255)
                                                                                 gATCGAAGATATGCCTTACGATGAAAACGGTACGACGGTAGATATCGTACTGATCTAGCTGGGCGTGTTGTGTAGTATGATTATTGGTGTGATTCTAGAAACCCATCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCa                                                     >  1:410094/1‑143 (MQ=255)
                                                                                   tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                   <  2:433212/143‑1 (MQ=255)
                                                                                      aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                                                >  2:416317/1‑143 (MQ=255)
                                                                                             gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAACGTCTCGTATGAAAATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGATATCGGGGACAAGACCAACGCGATGCTGa                                         >  2:284778/1‑143 (MQ=255)
                                                                                               cgtACGATGAAAGCGGTACGCCGGGAGACATGTTACTCAACCAGCTTGTCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGTTCAACGCCATGCTGaaa                                       <  2:9087/141‑1 (MQ=255)
                                                                                                ttACGATGAAAACGGTACGCCGGTAGACATCGTGCTGAATCAGCGGGGCGCACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGAGGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATTCTGAAAc                                      >  2:614481/1‑143 (MQ=255)
                                                                                                ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATGAACGCCATGCTGAAAc                                      >  2:141715/1‑143 (MQ=255)
                                                                                                     aTGAAAATTGTACGCTGGTAGACATGGTACTGAACCAGCTTGTCGTACCGTGTCGTATGAACATCGGTCACATCCTCGACACCCACCTGTGTATGTCTGCCAAAGGTATCGGCGACAAGATCAACGACATGCTGAAAcagcag                                 <  2:183310/143‑1 (MQ=255)
                                                                                                      tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                                <  1:489165/143‑1 (MQ=255)
                                                                                                           aCGGTACGCCGGTGGACTGCGTTACAAACCATGTGGGCGTTCTGCCCCAAATGAACACCGGTCAGATCCTCGAGCCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           <  1:734919/143‑1 (MQ=255)
                                                                                                              gTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                        <  1:614481/143‑1 (MQ=255)
                                                                                                               tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTGTCGGCGACAAGATCAACGCCATGGTGAAACAGCAGCGAGAAGtag                       >  1:992703/1‑141 (MQ=255)
                                                                                                                aCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCGGCAAGAAGTcgc                      <  1:350111/143‑1 (MQ=255)
                                                                                                                aCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                      <  1:128661/143‑1 (MQ=255)
                                                                                                                    cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGGTCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaaa                  >  1:554460/1‑143 (MQ=255)
                                                                                                                    cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGTGaaa                  <  2:639976/143‑1 (MQ=255)
                                                                                                                    cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaaa                  <  1:190571/143‑1 (MQ=255)
                                                                                                                        aGACATCGTACTGTGTCAGCTGTGCGTACCGTCTCGTAGTAACAGCGGTCAGAACCACGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc              <  1:88292/143‑1 (MQ=255)
                                                                                                                           cATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGcgcg           >  2:334306/1‑143 (MQ=255)
                                                                                                                            aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGa          >  1:606814/1‑143 (MQ=255)
                                                                                                                            aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGAGGAAACAGGAGCAAGAAGGGGCGAAGCTGCGCGa          >  2:206450/1‑143 (MQ=255)
                                                                                                                                    gAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCCGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATcc  >  1:238667/1‑143 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4184406‑4184680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A