Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F50 I1 R1 21 62.9 2410612 90.0% 2169550 142.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA98.5% 190.5 / NA 65P1100Q (CCG→CAG) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (32/32);  minor base T (0/1);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184406‑4184681
                                                                                                                                         |                                                                                                                                          
ttcctttAAACGCCGTATACAACCTGATGACAAGATAGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                       <  1:1050732/138‑1 (MQ=255)
  cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                     <  2:950787/143‑1 (MQ=255)
   ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCg                                                                                                                                    <  1:910533/143‑1 (MQ=255)
   ggTTAAACGCCGTATCCAGCATGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAACTGAGCg                                                                                                                                    >  2:274158/1‑143 (MQ=255)
    gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCACCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGt                                                                                                                                   >  1:275465/1‑143 (MQ=255)
     ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGAATTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                  >  1:690126/1‑143 (MQ=255)
        aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCg                                                                                                                               >  2:1187597/1‑143 (MQ=255)
         aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACAAGCTGGGCGTACCGt                                                                                                                              >  2:244407/1‑143 (MQ=255)
           gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtct                                                                                                                            >  1:173693/1‑143 (MQ=255)
            ccGTATCGATCCTGGTGACAAGACGGCAGGTCGTCACGGTACCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                           <  1:1046154/143‑1 (MQ=255)
             cGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTAAACCAGCTGGGCGTACCGTCTCg                                                                                                                          >  2:963685/1‑143 (MQ=255)
                 tCCAGCGTGGTGACAAGATCGCAGGGCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                      <  2:1066431/143‑1 (MQ=255)
                 tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                      <  2:565711/143‑1 (MQ=255)
                 tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                      >  1:1177990/1‑143 (MQ=255)
                    aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTGCTGAACCAGCTGGGCGTACCGTCTCGTATGAAc                                                                                                                   >  1:242754/1‑143 (MQ=255)
                    aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAc                                                                                                                   >  2:195177/1‑143 (MQ=255)
                    aGCCTGGTGACAAGATGGCAGATCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATgccc                                                                                                                   >  2:1055346/1‑140 (MQ=255)
                        tggtggCAAGAGGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               <  1:95896/143‑1 (MQ=255)
                        tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                               <  1:1020873/143‑1 (MQ=255)
                          gggACAAGATGGCAGGGCGGCACGGGAACAAGGGGGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCCTACGAGGAAAACGGTACGCCGGTAGACATCCTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                             <  1:257143/141‑1 (MQ=255)
                            gACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCa                                                                                                           >  1:779208/1‑143 (MQ=255)
                                 gATGGCAGGTCGTCGCGGTAACAAGGGTGTAACTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATcc                                                                                                      <  2:758590/143‑1 (MQ=255)
                                          tcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCCTATGAACATCGGGCAGATCCGCGAAAccc                                                                                             >  2:102888/1‑143 (MQ=255)
                                                gggTAACAAGGGTGTAATTTCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATTGCACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  2:789689/142‑1 (MQ=255)
                                                ccgTAACAAGGGTGTAATTTCTAAGATCCACCCGATCGAAGATATGCCCTACGACGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  1:583253/141‑1 (MQ=255)
                                                cGGTAACCAGGGTGTTATTTCTAAGATCAACCCGATCGAAGAGCAGCCTTACGATGACACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                       <  1:45321/143‑1 (MQ=255)
                                                 ggTAACAAGGGTGTAACTTCTAAGATCCACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTggg                                                                                      <  2:671159/143‑1 (MQ=255)
                                                  gTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGTCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGt                                                                                     <  2:363491/143‑1 (MQ=255)
                                                    aaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAACATCGAAACCCACCCTGGTAt                                                                                   >  2:1013179/1‑143 (MQ=255)
                                                      cAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                                 >  1:1150682/1‑143 (MQ=255)
                                                       aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                                >  2:385854/1‑143 (MQ=255)
                                                       aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCCGTAGACATCGTACTGAACCAGCTGGGCGTACCGCGCCGTCTGAACATCCGTCCGCCCCTCGCAACCCACCTcgggagcgc                                                                                >  2:299495/1‑137 (MQ=255)
                                                        aGGGCGTAATTCCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACTGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCt                                                                               <  1:418885/143‑1 (MQ=255)
                                                              tAATTTCTAAGACCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  1:777734/143‑1 (MQ=255)
                                                              gaagttCTAAGGTCAGCTCGATGGGAGATAGGCCGTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCTACCTGGGTATGGCTGCGaaa                                                                         <  2:1176824/139‑1 (MQ=255)
                                                               aaccacTACGACCAACCGGATCTAAGACATTCCTCACCATGAAAACGTTACGCCGGTACACATCGTACTCCACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                        <  1:357337/138‑1 (MQ=255)
                                                                 tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGCGCGTCCCGTCCCGTATCAACATCGGTCAGATCCTCGAAACCAACCTGGGTATGACTGCGAAAGGt                                                                      >  1:822568/1‑143 (MQ=255)
                                                                  ttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTa                                                                     >  1:753386/1‑143 (MQ=255)
                                                                   tCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAt                                                                    >  1:661974/1‑143 (MQ=255)
                                                                    cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTagc                                                                   >  2:114462/1‑141 (MQ=255)
                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTTGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                  <  1:224122/143‑1 (MQ=255)
                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCCACTGGGTCTGGCCGCGAAAgggagcc                                                                  >  2:335401/1‑138 (MQ=255)
                                                                       agacgaaCCCGATCGAAGATATGCCTCACGATGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGGAGGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGc                                                                <  2:1050327/138‑1 (MQ=255)
                                                                         gtCAACCCGAAAGAGGATACGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                              <  1:1065528/142‑1 (MQ=255)
                                                                         aTCAACCCGATCGAAGATATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                              <  2:328549/143‑1 (MQ=255)
                                                                            tgcccGAGTGAATATTTGCCTTACCATGAAAACGGTACGCCGGTAAACATCGTACTGACCCAGCTGGGCGCACCGTTTCCTATGAACTGCGGTCAGATTCTCGAATCCCACTTGGGTATTGCGGCGAAAGGTATCGGCGACaa                                                           <  2:706755/141‑1 (MQ=255)
                                                                            aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTCCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGTATGGCTGCGAAAGGTACCGGCgccca                                                           >  2:836955/1‑139 (MQ=255)
                                                                            aaCCCGATCGAAGATATGCCTTACGATGAAAACGGAACGCAGGTAAACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGACAGATCCTCGAAACCGAACTGGATATGGGTGCGGAAGGCACAGGCGAcca                                                           >  2:201689/1‑141 (MQ=255)
                                                                              cccGAACGAAGACATGCACTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                         <  1:99593/143‑1 (MQ=255)
                                                                                   tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGCCAAGATCAAc                                                    >  1:344020/1‑143 (MQ=255)
                                                                                   cccacgATCTGCCCTCCGATGAAAACGGTACGCCGGTAGACCTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    <  1:848100/138‑1 (MQ=255)
                                                                                      aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         >  2:716467/1‑119 (MQ=255)
                                                                                      aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  1:716467/119‑1 (MQ=255)
                                                                                        gATATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCAt                                               <  2:173693/143‑1 (MQ=255)
                                                                                         atatGCCTTACGATGAAAACAGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCATACACTCTCGTAGGAACATCGGTCAGATCCTCGAAACCCAACTGCGTATCGCTCCAAAAGATACCGGCAAACAGATAACCGCCAtc                                              >  2:231537/1‑142 (MQ=255)
                                                                                             gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTGTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGGGACAAGATCAACGCCCTGcggg                                          >  2:655591/1‑140 (MQ=255)
                                                                                                ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAACCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCGACGGAATGCTGaaaa                                       >  2:639302/1‑142 (MQ=255)
                                                                                                      tGAAAACGGTACGCCGGTAGACATTGTACTGAACCAGCTGGGCGTACCGTCTCGACTGCACACCGATCCGAAACGCGAAACCCACCCGGGTATGGCTGCGAAAGGTATCGGCGACAACATCACAAACATGCCGAAAAagcagc                                 >  2:121916/1‑143 (MQ=255)
                                                                                                         aaaCGGTACGCCGGTAGACATTGGACAGAACCTGTTGGGGGCACCAACCCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                              <  1:1187597/143‑1 (MQ=255)
                                                                                                         aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                              <  1:195177/143‑1 (MQ=255)
                                                                                                               tACGCCGGTAGCCATCGTAATGAACCAGCTGGGCGTACCGTCTCGTATGAACACCGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                        <  2:1188689/143‑1 (MQ=255)
                                                                                                                          aCATCGTACTCAACCAGCCGCGCTGTCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc             <  2:779208/143‑1 (MQ=255)
                                                                                                                            aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGa           >  1:692657/1‑143 (MQ=255)
                                                                                                                              cGTACGGAGCCAGCGGGGCGTAGCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCTCCCTAGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt         <  1:274158/143‑1 (MQ=255)
                                                                                                                                     aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAACTCATCCa  >  2:697727/1‑143 (MQ=255)
                                                                                                                                         |                                                                                                                                          
GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184406‑4184681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A