Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A23 F67 I0 R1
|
35 |
27.8 |
1096780 |
91.1% |
999166 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,184,543 |
C→A |
100% |
P1100Q (CCG→CAG) |
rpoB → |
RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,184,543 | 0 | C | A | 100.0%
| 124.8
/ NA
| 40 | P1100Q (CCG→CAG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (19/21); total (19/21) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA > NC_000913/4184413‑4184677
|
aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAAATAGGGCGTAATGCCGACCAACCAATTTTAATG‑ACATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAcc > 1:401292/1‑143 (MQ=255)
ccggacccAGCCGGGTGACAAGATGGTAGGTCGTCACGGTAACGAGGGTGTAATTTCTA‑AGATCTACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc < 2:442153/137‑1 (MQ=255)
ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCTGGGCGTACCGtctc > 2:340273/1‑143 (MQ=255)
aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGATATTCTAGGTTAGTTTGTACTGCACCATCTGCGCGTACCGTCTCGTAt > 1:396216/1‑143 (MQ=255)
tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg < 2:33896/143‑1 (MQ=255)
acaaggcTGGTGACAAGATCGCGGGTCGGCACGGTAACAAGGGTGTAAGTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACATCCTACTGAACCAGCCGGGCGTACCGTCTCGTATg < 2:543743/137‑1 (MQ=255)
ccAGCCTGCTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa < 1:311381/143‑1 (MQ=255)
ccTGTTGACAAGATTGCAGGTCGTCTCGGTGACAAGCGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAt < 1:67684/143‑1 (MQ=255)
ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCgg > 1:31822/1‑143 (MQ=255)
aCAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGGACTGAACCAGCTGGGCGTAGCGTGTCGTATGAACATCGGTGAg > 1:216446/1‑143 (MQ=255)
aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt > 1:108977/1‑143 (MQ=255)
agagGGCAGGCCGTCACGGTAACAGGGGTGTAATTTCCA‑AGACCACCCCCATCGAATATATGCCTTACGATGCAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATc < 1:310897/143‑1 (MQ=255)
aGATCGCAGGTCGTCACGGTAACAAGGGTGGAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGATATCGTACTGAACCAGCTGCGCGTACCGTCTCGTATGAACATCGGTCAGATc < 2:286315/143‑1 (MQ=255)
aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAGCCGGTACGCAGATAGTCTTGGTATTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt < 2:388806/143‑1 (MQ=255)
ggTCGGCACGGTAACAAGGGTGTAATTTCTA‑AGAAGAGTGCGATCGAAGCTATGCCTTACGATGAAAACGGTACTCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAc < 2:112155/143‑1 (MQ=255)
gtcACGGTAACAAGGGGTTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAATGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc < 2:69243/143‑1 (MQ=255)
cACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt < 1:428759/143‑1 (MQ=255)
aaGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc < 1:473048/143‑1 (MQ=255)
gggTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTg > 1:272620/1‑143 (MQ=255)
aaTTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTGGTATGAACATCGGTCAGATGCTCGAAACCGACGGGGGGATGGCCGCGAAAg > 1:29349/1‑143 (MQ=255)
tA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg > 1:171815/1‑143 (MQ=255)
aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGACACCCACCTGGGTATGGCTGCGAAAGGTATCGGCgc > 1:547716/1‑142 (MQ=255)
aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGTGCGTCTGCGAATGGTATCGGCGa > 2:212758/1‑143 (MQ=255)
tCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGAGTATGGCTGCGAAAGGTATCGGCGAc < 1:79193/143‑1 (MQ=255)
aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa > 1:102806/1‑143 (MQ=255)
cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTATCGGCGACACGa > 1:153200/1‑143 (MQ=255)
tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc > 1:153802/1‑143 (MQ=255)
gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGACTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTCTGGCTGCGAACGGTATCGGCGCCCAGAGCAACga > 2:153085/1‑142 (MQ=255)
aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACACACCTGGGTATGGCTGCGAAAGGTATCGGCGACGAGATCAACGcc > 1:359957/1‑143 (MQ=255)
aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGCACATCGGTCAGATCCGCGAAACCCACCTGGGAATGGCTGCGAAAGGAATCGGAGACAAGACCCACGcc > 2:55967/1‑143 (MQ=255)
cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTAAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa < 2:445520/143‑1 (MQ=255)
ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaacc > 2:400693/1‑141 (MQ=255)
gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca < 2:31822/143‑1 (MQ=255)
gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca < 1:169902/143‑1 (MQ=255)
aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa < 1:83775/143‑1 (MQ=255)
gtctgCCGGTAGCCCTCGTACTGAACCAGCTTGCCGTACTGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGTTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc < 2:225132/139‑1 (MQ=255)
gACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg < 2:401292/143‑1 (MQ=255)
tggTACTGGACCAGCTGGGCCTACCGTCTCGTATGTACATCGCTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa < 2:142984/141‑1 (MQ=255)
tcttACTGTACCAGCCAGGCGTACCGTCTCAAGTTTAGATCGGTCAGAGCCTCGAAACCCACATGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa < 2:357104/140‑1 (MQ=255)
aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa > 1:474748/1‑143 (MQ=255)
|
AAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTA‑AGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA > NC_000913/4184413‑4184677
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
