Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F20 I0 R1
|
33 |
26.4 |
1163560 |
84.9% |
987862 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,184,543 |
C→A |
100% |
P1100Q (CCG→CAG) |
rpoB → |
RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,184,543 | 0 | C | A | 100.0%
| 75.1
/ ‑6.7
| 32 | P1100Q (CCG→CAG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (18/13); minor base G (0/1); total (18/14) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG > NC_000913/4184404‑4184682
|
cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTCCTGAACCAGcc > 2:206671/1‑142 (MQ=255)
cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATACCTTACGAAGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCt > 1:492473/1‑143 (MQ=255)
gCGGTTAAACGCCGTATCCAGCCTGGTGACAAGCTGGCCGGTCGTCACGGTAACAACGGTGTAATTTCTAAGATCAACACGATCGAAGATATGCCATACGATGAAAACGGTACGCCGGTACACATCGTACTGAACCAGCTggg > 1:396015/1‑143 (MQ=255)
gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTCACAAGGGTGTAATTTCTAAGATAAACAAGATAGAAGATATGACTTACGATGAAAACGGTACGCGGGTAGACATCGTACTGAACCAGCTGGGCGt > 1:231543/1‑143 (MQ=255)
aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGCTCGAAGATATGCCTTACGATGAAAACGGTACACCGGTAGACATCCTACTGAACCAACTGGGCGCACCGt > 2:540835/1‑143 (MQ=255)
ccGTATCTAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc < 1:207614/143‑1 (MQ=255)
tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCGTACGATGAAAACGGTACGCCGGTAGACACCGCACTGAACCAGCTGGGCGTACCGTCTCGTACCAACATCGGTc > 1:236707/1‑143 (MQ=255)
accgCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt < 2:56101/140‑1 (MQ=255)
aTGGCAGGGCGTGACGGGAACAAGGGTGTAATTTCTAAGAACGACCCGATCGAAGAGATGCCTTACGATGAAATCGGTGCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt < 1:151373/143‑1 (MQ=255)
atgtcgtcACGGAAACAAGAGTGTAATAGCTAACATCGTCGCGAACGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa < 1:122702/141‑1 (MQ=255)
cgtggTAACAATGTTGTAATTTTTTATATTAACTAGATCGAAGATAAGGCTTACGACGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt < 1:190412/140‑1 (MQ=255)
aCAGGGGTGTAATTTGTGAGAGCAACCCGAGCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATg < 2:367231/143‑1 (MQ=255)
tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa < 1:55193/143‑1 (MQ=255)
tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGAGGGGTGCGcaa > 1:556612/1‑143 (MQ=255)
aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACAGTCTCGTATGAACATAGGTCAGATAATCGAAACACACCTGGGTATGGCTGCGAAAgg > 1:480706/1‑143 (MQ=255)
cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAACCACCTAGGTATGGCTACGAAAAGTATc > 1:172847/1‑143 (MQ=255)
tAAGATCAACCCGATCGAAGATATGCCTTACTATGAACGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAACACCCCTGGTATGGCTGCGAAAGGTATCg < 2:197594/143‑1 (MQ=255)
tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCCGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATcc > 2:541467/1‑142 (MQ=255)
tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAAAGGTACGCCGGTAGACATCGTACTAAACCAGCCGGGCGTACCGTATCGTATGAACATCGGTCAGATAGTCGAAACCCACCTGGGTACCGCTCCGaaacgcacag > 2:556938/1‑136 (MQ=255)
aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa < 1:448550/143‑1 (MQ=255)
tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGCAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc > 2:96067/1‑143 (MQ=255)
atatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACATGAGTATGGCTGCGAAAGGTATCGGACACAAAATCAACGCCATg > 1:54035/1‑143 (MQ=255)
atatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCACGAAACCAACCTGGGAATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg > 1:138178/1‑143 (MQ=255)
tatGTCGTACGATGAAAAGGGTACGCCGGGAGACAGCGTACTGAAGCGGCTGGGCGTACCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc < 2:160327/143‑1 (MQ=255)
atGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTATCGGCGACAAGATCACCGCCATGCt > 1:543978/1‑143 (MQ=255)
ctaTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGCGCGTACCGTCTCTTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc < 2:267219/141‑1 (MQ=255)
gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAAACGATGAATGCCATGCTGAAAcagaaacc > 2:17409/1‑138 (MQ=255)
acttcGGTAGACATTGTACTGAATCAGCTGGGCGTACCGTCTCGGATGAACAGCGGTCAGAGCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc < 1:487574/139‑1 (MQ=255)
gcttgTAGACATCGTACTGACCCAGGGGGGAGTACCGTCTCGTATGGACATCGGTCAGAGCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa < 1:274356/139‑1 (MQ=255)
aGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc < 1:540037/143‑1 (MQ=255)
gACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg > 2:125871/1‑143 (MQ=255)
aCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTGGCTAAACTGCGCGAATTCATCCAg > 1:476433/1‑143 (MQ=255)
|
CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG > NC_000913/4184404‑4184682
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
