breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 94,850 T→C 3.3% V67V (GTT→GTC murF → UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
JC 136,615 Δ5 bp 3.5% coding (299‑303/348 nt) yacC ← PulS_OutS family protein
RA 165,960 C→T 3.2% Q411* (CAG→TAG)  mrcB → fused glycosyl transferase and transpeptidase
RA 201,088 Δ1 bp 3.0% coding (118/1026 nt) lpxD → UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 286,391 Δ1 bp 3.4% coding (997/1383 nt) yagG → CP4‑6 prophage, putative sugar transporter
RA 363,169 A→G 3.2% L4S (TTA→TCA)  lacY ← lactose permease
RA 439,812 A→G 3.7% H122H (CAT→CAC dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
RA 572,774 G→A 3.3% Q103Q (CAG→CAA ybcN → DLP12 prophage, uncharacterized protein
RA 623,367 Δ1 bp 2.9% coding (1068/1251 nt) entS → enterobactin exporter, iron‑regulated
RA 701,374 C→T 7.4% R74H (CGC→CAC)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 701,554 A→G 5.2% L14P (CTC→CCC)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 702,331 Δ1 bp 6.5% coding (421/1149 nt) nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 736,928 T→G 3.5% pseudogene (104/1137 nt) ybfL → pseudogene, DDE domain transposase family,putative factor, Not classified, putative receptor protein
RA 772,687 T→C 3.7% S410S (AGT→AGC cydA → cytochrome d terminal oxidase, subunit I
RA 851,329 Δ1 bp 2.9% coding (1269/1584 nt) opgE ← OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 1,047,148 Δ1 bp 2.7% coding (798/2097 nt) gfcD ← putative O‑antigen capsule production periplasmic protein
RA 1,051,766 A→G 3.9% intergenic (+93/‑81) cspG → / → ymcE cold shock protein homolog, cold‑inducible/cold shock gene
RA 1,158,171 Δ1 bp 5.9% coding (303/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
JC 1,159,286:1 +GTA 21.4% coding (1418/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
RA 1,269,474 A→T 4.0% intergenic (+85/+229) rdlA → / ← ldrB sRNA antisense regulator affects LdrA translation, proposed addiction module in LDR‑A repeat, with toxic peptide LdrA/toxic polypeptide, small
RA 1,269,884 C→T 3.7% noncoding (27/66 nt) rdlB → sRNA antisense regulator affects LdrB translation, proposed addiction module in LDR‑B repeat, with toxic peptide LdrB
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
JC 1,326,599 Δ36 bp 9.4% coding (1854‑1889/1896 nt) yciQ → enhancer of membrane protein expression, putative inner membrane protein
RA 1,466,201 T→C 5.1% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,466,210 G→A 4.5% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,466,258 G→C 5.0% pseudogene (867/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,490,727 C→T 3.6% intergenic (‑14/‑175) gapC ← / → cybB pseudogene, GAP dehydrogenase, glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)/cytochrome b561
RA 1,560,522 Δ1 bp 3.4% coding (408/1023 nt) ddpB ← D,D‑dipeptide ABC transporter permease
RA 1,708,805 T→C 21.4% R629R (CGT→CGC rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,970,383 Δ1 bp 3.2% coding (602/1602 nt) tap ← methyl‑accepting protein IV
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,133,164:1 +G 3.2% coding (234/840 nt) wcaA ← putative glycosyl transferase
RA 2,156,595:1 +T 2.7% coding (1333/3123 nt) mdtB → multidrug efflux system, subunit B
RA 2,156,595:2 +C 2.7% coding (1333/3123 nt) mdtB → multidrug efflux system, subunit B
RA 2,204,384 Δ1 bp 4.2% intergenic (+97/‑210) yehK → / → yehL uncharacterized protein/putative hexameric AAA+ MoxR family ATPase
RA 2,304,520 G→T 6.6% intergenic (+129/+586) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,521 G→C 6.1% intergenic (+130/+585) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,535 A→G 6.9% intergenic (+144/+571) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,538 C→T 5.3% intergenic (+147/+568) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,547 C→T 6.4% intergenic (+156/+559) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,565 C→T 6.4% intergenic (+174/+541) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,791 C→T 8.5% intergenic (+400/+315) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,796 T→C 13.1% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,314,714 C→A 10.4% A410E (GCG→GAG)  rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
RA 2,342,529 T→C 3.5% K547E (AAA→GAA)  yfaL ← adhesin
RA 2,354,399 A→G 4.9% D46G (GAT→GGT)  glpB → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit
RA 2,427,316 Δ1 bp 3.1% coding (474/783 nt) argT ← lysine/arginine/ornithine transporter subunit
JC 2,765,454 (TATGGCAC)6→5 6.8% intergenic (‑303/+50) yfjL ← / ← yfjM CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage, uncharacterized protein
RA 2,784,549 T→C 3.5% pseudogene (461/1374 nt) ypjC ← pseudogene
RA 2,804,603 T→C 3.3% intergenic (+144/‑210) nrdF → / → proV ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein
RA 3,169,993 C→T 2.5% G56G (GGC→GGT qseB → quorum sensing DNA‑binding response regulator in two‑component regulatory system with QseC
RA 3,579,887 A→T 3.2% Y306* (TAT→TAA yhhX ← putative oxidoreductase
RA 3,608,681 T→C 11.7% intergenic (+33/+69) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,742,053 C→T 2.8% G160D (GGC→GAC)  yiaJ ← transcriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon
RA 3,812,319 T→C 3.7% L80L (TTA→TTG yicR ← UPF0758 family protein
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
MC JC 3,909,311 Δ774 bp 100% [pstA][pstC] [pstA], [pstC]
RA 3,958,514 T→A 3.2% D182E (GAT→GAA ilvC → ketol‑acid reductoisomerase, NAD(P)‑binding
JC 4,001,644 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,171,647 A→C 100% noncoding (12/120 nt) rrfB → 5S ribosomal RNA of rrnB operon
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
JC 4,204,931:1 (CAC)2→3 3.2% coding (1000/1290 nt) purD ← phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase
RA 4,283,001 G→A 3.1% P25L (CCA→CTA)  yjcF ← pentapeptide repeats protein
RA 4,295,833 T→C 3.8% intergenic (+40/+604) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,189 A→G 44.7% intergenic (+396/+248) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→C 40.9% intergenic (+397/+247) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,325,949 A→G 5.9% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,326,248 C→T 3.9% intergenic (‑506/+152) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,502,370:1 +T 2.5% coding (1063/1329 nt) insG ← IS4 transposase
RA 4,502,370:2 +C 2.5% coding (1063/1329 nt) insG ← IS4 transposase
RA 4,635,825 Δ1 bp 2.8% coding (304/474 nt) creA → putative periplasmic protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4110683 4111524 842 54 [52] [52] 54 [cdh]–tpiA [cdh], tpiA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 243009123 (0.960)5 (0.040) 5/496 NT 3.9% coding (295/2445 nt) fadE acyl coenzyme A dehydrogenase
?NC_000913 = 243192 128 (1.030)coding (112/2445 nt) fadE acyl coenzyme A dehydrogenase
* ? NC_000913 257908 =NA (NA)144 (1.130) 87/510 NT 98.6% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 2 (0.020)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 273955 =NA (NA)5 (0.040) 5/510 NT 6.0% intergenic (+1/+146) ykfC/insH1 CP4‑6 prophage, conserved protein,Phage or Prophage Related/IS5 transposase and trans‑activator
?NC_000913 = 702474 78 (0.610)coding (278/1149 nt) nagA N‑acetylglucosamine‑6‑phosphate deacetylase
* ? NC_000913 = 366099108 (0.850)3 (0.020) 3/488 NT 2.7% coding (207/3075 nt) lacZ beta‑D‑galactosidase
?NC_000913 = 366105 113 (0.920)coding (201/3075 nt) lacZ beta‑D‑galactosidase
* ? NC_000913 391708 =NA (NA)3 (0.020)
+GTGCC
3/500 NT NA pseudogene (1458/1458 nt) yaiT pseudogene, autotransporter family,putative structure, Not classified, interrupted by IS3, putative flagellin structural protein
?NC_000913 = 392963 NA (NA)intergenic (‑62/‑7) insE1/yaiT IS3 transposase A/pseudogene, autotransporter family,putative structure, Not classified, interrupted by IS3, putative flagellin structural protein
* ? NC_000913 453557 =109 (0.850)4 (0.030) 4/496 NT 3.7% intergenic (‑12/+32) ampG/yajG muropeptide transporter/putative lipoprotein
?NC_000913 453577 = 104 (0.840)intergenic (‑32/+12) ampG/yajG muropeptide transporter/putative lipoprotein
* ? NC_000913 702318 =101 (0.790)5 (0.040) 5/510 NT 3.7% coding (434/1149 nt) nagA N‑acetylglucosamine‑6‑phosphate deacetylase
?NC_000913 4502091 = 158 (1.240)intergenic (+501/+13) yjgZ/insG uncharacterized protein/IS4 transposase
* ? NC_000913 934998 =112 (0.880)3 (0.020) 3/500 NT 2.8% coding (1775/3990 nt) ftsK DNA translocase at septal ring sorting daughter chromsomes
?NC_000913 935033 = 97 (0.770)coding (1810/3990 nt) ftsK DNA translocase at septal ring sorting daughter chromsomes
* ? NC_000913 1159285 =81 (0.630)4 (0.030) 4/506 NT 4.7% coding (1417/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component
?NC_000913 = 1469238 NA (NA)intergenic (‑89/‑3) insC1/ydbA IS2 repressor TnpA/pseudogene, autotransporter homolog, interrupted by IS2 and IS30
* ? NC_000913 = 115929179 (0.620)8 (0.060) 8/510 NT 9.2% coding (1423/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component
?NC_000913 = 1651548 NA (NA)intergenic (+11/‑3) insD1/intQ IS2 transposase TnpB,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related/pseudogene, Qin prophage, phage integrase family,Phage or Prophage Related
* ? NC_000913 1207790 =79 (0.620)35 (0.290) 32/478 NT 30.4% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 86 (0.720)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 120780581 (0.630)32 (0.270) 26/478 NT 28.3% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 86 (0.720)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =2 (0.020)124 (0.970) 79/510 NT 98.4% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 1314673 =89 (0.700)3 (0.020) 3/492 NT 3.5% intergenic (+15/+45) ompW/yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
?NC_000913 1314694 = 80 (0.650)intergenic (+36/+24) ompW/yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
* ? NC_000913 1503511 =104 (0.810)4 (0.030) 4/500 NT 3.6% pseudogene (139/432 nt) insP pseudogene, IS609 transposase A,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
?NC_000913 1504443 = 111 (0.890)coding (727/1149 nt) insQ IS609 transposase B
* ? NC_000913 1979486 =0 (0.000)83 (0.650) 58/510 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)113 (0.880) 70/510 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 = 2290110NA (NA)4 (0.030) 4/508 NT 4.1% intergenic (‑31/+2) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
?NC_000913 2976723 = 94 (0.740)coding (127/1032 nt) galR galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor
* ? NC_000913 = 2498106133 (1.040)4 (0.030) 3/488 NT 3.8% coding (188/1239 nt) alaC glutamate‑pyruvate aminotransferase, glutamic‑pyruvic transaminase (GPT), alanine transaminase
?NC_000913 = 2498313 77 (0.630)intergenic (‑20/‑356) alaC/ypdA glutamate‑pyruvate aminotransferase, glutamic‑pyruvic transaminase (GPT), alanine transaminase/sensor kinase regulating yhjX, pyruvate‑responsive YpdAB two‑component system
* ? NC_000913 = 2608067126 (0.990)6 (0.050) 6/496 NT 4.5% coding (1153/1581 nt) hyfF hydrogenase 4, membrane subunit
?NC_000913 = 2608077 133 (1.070)coding (1163/1581 nt) hyfF hydrogenase 4, membrane subunit
* ? NC_000913 = 2713827109 (0.850)3 (0.020) 3/488 NT 2.9% coding (934/1335 nt) srmB ATP‑dependent RNA helicase
?NC_000913 = 2713906 94 (0.770)coding (1013/1335 nt) srmB ATP‑dependent RNA helicase
* ? NC_000913 2796363 =132 (1.030)5 (0.040) 5/490 NT 3.8% coding (422/450 nt) ygaU uncharacterized protein
?NC_000913 2796384 = 124 (1.010)coding (401/450 nt) ygaU uncharacterized protein
* ? NC_000913 2833078 =141 (1.100)4 (0.030) 4/492 NT 3.0% coding (605/1440 nt) norV anaerobic nitric oxide reductase flavorubredoxin
?NC_000913 2833217 = 126 (1.020)coding (744/1440 nt) norV anaerobic nitric oxide reductase flavorubredoxin
* ? NC_000913 = 3041641142 (1.110)4 (0.030) 4/486 NT 3.1% coding (331/981 nt) ygfZ iron‑sulfur cluster repair protein, plumbagin resistance
?NC_000913 = 3042743 116 (0.950)coding (404/660 nt) yqfA hemolysin III family HylIII inner membrane protein
* ? NC_000913 3419953 =143 (1.120)5 (0.040) 3/494 NT 3.6% pseudogene (849/960 nt) yhdW pseudogene, ABC transporter periplasmic binding protein family,putative transport, Not classified, putative periplasmic binding transport protein, periplasmic‑binding component of ABC superfamily
?NC_000913 3420126 = 127 (1.030)intergenic (+62/‑6) yhdW/yhdX pseudogene, ABC transporter periplasmic binding protein family,putative transport, Not classified, putative periplasmic binding transport protein, periplasmic‑binding component of ABC superfamily/putative amino acid ABC transporter permease
* ? NC_000913 4295831 =NA (NA)10 (0.090) 5/432 NT NA intergenic (+38/+606) gltP/yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
?NC_000913 = 4296395 NA (NA)noncoding (562/602 nt) RIP321 (repetitive extragenic palindromic) element, contains 11 REP sequences and 4 IHF sites RIP321 (repetitive extragenic palindromic) element, contains 11 REP sequences and 4 IHF sites
* ? NC_000913 4319452 =150 (1.170)3 (0.020) 3/490 NT 2.8% coding (156/846 nt) phnJ carbon‑phosphorus lyase, SAM‑dependent
?NC_000913 4320123 = 66 (0.540)coding (542/1065 nt) phnI ribophosphonate triphosphate synthase complex putative catalytic subunit
* ? NC_000913 4325843 =NA (NA)15 (0.120)
+AAC
5/504 NT NA noncoding (41/583 nt) REP325 (repetitive extragenic palindromic) element, contains 12 REP sequences REP325 (repetitive extragenic palindromic) element, contains 12 REP sequences
?NC_000913 = 4326339 NA (NA)noncoding (537/583 nt) REP325 (repetitive extragenic palindromic) element, contains 12 REP sequences REP325 (repetitive extragenic palindromic) element, contains 12 REP sequences