breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE512,6430AG52.2% 58.3 / 313.4 247intergenic (+711/‑6052)recR/speA_2Recombination protein RecR/Arginine decarboxylase
*USA300TCH1516_ALE111,5120AG35.0% 234.5 / 186.6 200S194S (TCA→TCGUSA300HOU_RS00520putative lipoprotein
*USA300TCH1516_ALE111,5160CA33.5% 272.7 / 165.2 195Q196K (CAA→AAA) USA300HOU_RS00520putative lipoprotein
*USA300TCH1516_ALE1,435,0650CG24.5% 244.3 / 25.1 107A141G (GCT→GGT) dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
*USA300TCH1516_ALE1,435,0740GA23.4% 246.6 / 19.8 111G144D (GGT→GAT) ‡dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
*USA300TCH1516_ALE1,435,0750TA22.5% 255.7 / 17.5 111G144G (GGT→GGA) ‡dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
*USA300TCH1516_ALE1,435,0690AC22.4% 215.7 / 12.0 108T142T (ACA→ACCdapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
*USA300TCH1516_ALE1,435,0810TA22.4% 236.2 / 21.3 110N146K (AAT→AAAdapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
*USA300TCH1516_ALE1,435,0730GA22.0% 237.4 / 22.6 111G144S (GGT→AGT) ‡dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
*USA300TCH1516_ALE1,435,0630TC21.3% 214.5 / 28.8 109R140R (CGT→CGCdapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
*USA300TCH1516_ALE1,435,0640GC20.6% 264.2 / 20.7 107A141P (GCT→CCT) dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
*USA300TCH1516_ALE1,435,0710CT20.0% 247.4 / 14.3 111T143I (ACT→ATT) dapH2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase

Marginal new junction evidence (lowest skew 10 of 53 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE = 1205323102 (0.780)25 (0.260)
+38 bp
8/218 6.1 21.8% intergenic (‑204/‑365) USA300HOU_RS15290/lspA hypothetical protein/Lipoprotein signal peptidase
?USA300TCH1516_ALE 2478252 = 141 (1.080)intergenic (+139/‑436) USA300HOU_RS12760/USA300HOU_RS12765 hypothetical protein/hypothetical protein
* ? USA300TCH1516_ALE = 1205323102 (0.780)25 (0.260)
+38 bp
8/218 6.1 25.4% intergenic (‑204/‑365) USA300HOU_RS15290/lspA hypothetical protein/Lipoprotein signal peptidase
?USA300TCH1516_ALE = 2072043 96 (0.740)intergenic (+538/+35) USA300HOU_RS10445/USA300HOU_RS10450 hypothetical protein/hypothetical protein
* ? USA300TCH1516_ALE = 1205323102 (0.780)25 (0.260)
+38 bp
8/218 6.1 24.9% intergenic (‑204/‑365) USA300HOU_RS15290/lspA hypothetical protein/Lipoprotein signal peptidase
?USA300TCH1516_ALE = 1443739 NA (NA)intergenic (‑312/+41) USA300TCH1516_01342/brnQ_3 hypothetical protein/Branched‑chain amino acid transport system 2 carrier protein
* ? USA300TCH1516_ALE = 1205323102 (0.780)25 (0.260)
+38 bp
8/218 6.1 27.0% intergenic (‑204/‑365) USA300HOU_RS15290/lspA hypothetical protein/Lipoprotein signal peptidase
?USA300TCH1516_ALE 1426517 = 81 (0.620)intergenic (‑248/+724) pstS/cvfB Phosphate‑binding protein PstS/Conserved virulence factor B
* ? USA300TCH1516_ALE = 516939NA (NA)8 (0.060) 7/282 7.9 NA intergenic (+5007/‑1756) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE = 516944 NA (NA)intergenic (+5012/‑1751) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
* ? USA300TCH1516_ALE 633406 =116 (0.890)6 (0.050) 6/282 8.3 4.8% intergenic (+130/‑414) proP/yhfT Proline/betaine transporter/putative acyl‑‑CoA ligase YhfT
?USA300TCH1516_ALE 633436 = 128 (1.030)intergenic (+160/‑384) proP/yhfT Proline/betaine transporter/putative acyl‑‑CoA ligase YhfT
* ? USA300TCH1516_ALE 1454018 =96 (0.740)8 (0.070) 5/270 8.6 8.1% intergenic (‑97/+187) odhA/arlS 2‑oxoglutarate dehydrogenase E1 component/Signal transduction histidine‑protein kinase ArlS
?USA300TCH1516_ALE 1454048 = 94 (0.790)intergenic (‑127/+157) odhA/arlS 2‑oxoglutarate dehydrogenase E1 component/Signal transduction histidine‑protein kinase ArlS
* ? USA300TCH1516_ALE 2440041 =105 (0.810)4 (0.030) 4/270 9.1 3.8% intergenic (+2/+64) USA300HOU_RS12570/malP hypothetical protein/PTS system maltose‑specific EIICB component
?USA300TCH1516_ALE 2440099 = 106 (0.890)intergenic (+60/+6) USA300HOU_RS12570/malP hypothetical protein/PTS system maltose‑specific EIICB component
* ? USA300TCH1516_ALE 399691 =119 (0.910)5 (0.040) 4/272 9.1 4.4% coding (1712/1713 nt) efeU Ferrous iron permease EfeU
?USA300TCH1516_ALE 399718 = 108 (0.900)intergenic (+26/+66) efeU/tatC2 Ferrous iron permease EfeU/Sec‑independent protein translocase protein TatCy
* ? USA300TCH1516_ALE = 800928158 (1.210)5 (0.040) 4/280 9.3 3.4% intergenic (+62/‑289) hisC_1/USA300HOU_RS03915 Histidinol‑phosphate aminotransferase/Putative 5'(3')‑deoxyribonucleotidase
?USA300TCH1516_ALE 801948 = 134 (1.080)intergenic (+189/+435) USA300HOU_RS03915/USA300HOU_RS03920 Putative 5'(3')‑deoxyribonucleotidase/Putative lipid kinase