breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,736 A→T intergenic (+105/‑87) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,903 T→A D27E (GAT→GAA folA → type 3 dihydrofolate reductase
RA 50,280 T→C F153S (TTT→TCT)  folA → type 3 dihydrofolate reductase
RA 108,362 A→G F242S (TTC→TCC)  zapD ← cell division protein ZapD
RA 115,488 T→C F90S (TTC→TCC)  ampD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD
RA 115,628 A→C T137P (ACG→CCG)  ampD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD
RA 139,618 A→G E118G (GAA→GGA)  yadG → ABC transporter ATP‑binding protein
RA 221,335 T→C noncoding (1078/1542 nt) BW25113_RS01010 → 16S ribosomal RNA
RA 231,042 A→C G76G (GGT→GGG gloB ← hydroxyacylglutathione hydrolase
RA 264,689 (A)5→4 intergenic (‑18/+311) ykfA ← / ← perR GTPase family protein/LysR substrate‑binding domain‑containing protein
RA 297,509 G→C G30G (GGC→GGG paoB ← aldehyde oxidoreductase FAD‑binding subunit PaoB
RA 340,444 (A)5→4 coding (558/816 nt) BW25113_RS01680 → hypothetical protein
RA 354,025 T→C E126G (GAA→GGA)  cynR ← transcriptional regulator CynR
RA 386,045 T→C pseudogene (339/1527 nt) BW25113_RS01915 → outer membrane autotransporter barrel domain‑containing protein
RA 444,519 G→A R527C (CGT→TGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 465,464 T→C S380P (TCG→CCG)  mdlA → SmdA family multidrug ABC transporter permease/ATP‑binding protein
RA 508,601 (C)6→7 coding (570/1293 nt) ybaT → APC family permease
RA 539,975 A→G D258D (GAT→GAC allC ← allantoate deiminase
RA 554,427 A→G intergenic (+217/‑3) fimA → / → fimC type 1 fimbrial major subunit FimA/type 1 fimbria chaperone FimC
RA 568,752 A→G G71G (GGA→GGG ybcO → DUF1364 domain‑containing protein
RA 579,943 (T)8→7 intergenic (+57/+193) appY → / ← ompT DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT
RA 601,986 A→G F285S (TTC→TCC)  ybdK ← YbdK family carboxylate‑amine ligase
RA 661,892 A→C G594G (GGT→GGG mrdA ← peptidoglycan DD‑transpeptidase MrdA
RA 674,442 T→G T141P (ACT→CCT)  ybeT ← tetratricopeptide repeat protein
RA 950,036 (T)5→4 intergenic (‑114/+292) focA ← / ← ycaO formate transporter FocA/30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 951,809 T→G T94P (ACC→CCC)  ycaO ← 30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 979,260 T→C I52T (ATC→ACC)  gloC → hydroxyacylglutathione hydrolase GloC
RA 1,021,663 (A)5→4 coding (1737/2055 nt) helD → DNA helicase IV
RA 1,060,988 (A)6→5 intergenic (+240/‑53) yccE → / → agp YccE family protein/bifunctional glucose‑1‑phosphatase/inositol phosphatase
RA 1,062,210 T→C G390G (GGT→GGC agp → bifunctional glucose‑1‑phosphatase/inositol phosphatase
RA 1,065,426 T→G T129P (ACC→CCC)  rutF ← pyrimidine utilization flavin reductase protein F
RA 1,076,727 (T)5→4 intergenic (+458/‑85) putP → / → BW25113_RS05295 sodium/proline symporter PutP/FTR1 family protein
RA 1,110,952 T→C intergenic (‑6/+166) mdtG ← / ← lpxL multidrug efflux MFS transporter MdtG/kdo(2)‑lipid IV(A) lauroyltransferase
RA 1,240,670 Δ1 bp coding (55/441 nt) BW25113_RS06225 → hypothetical protein
RA 1,259,173 A→G T2A (ACC→GCC)  hemA → glutamyl‑tRNA reductase
RA 1,276,251 Δ1 bp coding (932/3744 nt) narG → nitrate reductase subunit alpha
RA 1,300,524 Δ1 bp coding (504/1005 nt) oppF → murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF
RA 1,320,565 G→A Q521Q (CAG→CAA yciQ → DUF2207 domain‑containing protein
RA 1,325,886 T→C G194G (GGT→GGC topA → type I DNA topoisomerase
RA 1,331,016 A→G Y310C (TAT→TGT)  acnA → aconitate hydratase AcnA
RA 1,392,826 T→C Q18Q (CAA→CAG uspE ← universal stress protein UspE
RA 1,394,163 G→A L111L (CTG→TTG)  ogt ← methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,402,000 A→G I18T (ATC→ACC)  dgcM ← diguanylate cyclase DgcM
RA 1,417,788 (C)6→7 coding (69/120 nt) BW25113_RS26130 ← hypothetical protein
RA 1,423,602 T→C R99R (CGT→CGC stfR → prophage tail fiber N‑terminal domain‑containing protein
RA 1,431,536 T→C E1169G (GAA→GGA)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,434,377 A→G F222S (TTC→TCC)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,454,774 A→G D16G (GAT→GGT)  paaI → hydroxyphenylacetyl‑CoA thioesterase PaaI
RA 1,499,566 A→G S258P (TCC→CCC)  ydcO ← BenE family transporter YdcO
RA 1,502,076 A→G N347D (AAC→GAC)  rlhA → 23S rRNA 5‑hydroxycytidine C2501 synthase
RA 1,503,965 T→C D67D (GAT→GAC hicB → type II toxin‑antitoxin system antitoxin HicB
RA 1,524,815 T→C intergenic (‑54/‑28) BW25113_RS25600 ← / → BW25113_RS07655 protein YncO/ISAs1 family transposase
RA 1,531,275 G→A L98L (CTG→TTG)  narW ← nitrate reductase molybdenum cofactor assembly chaperone
RA 1,547,935 A→G V54A (GTA→GCA)  adhP ← alcohol dehydrogenase AdhP
RA 1,558,439 A→G Y518H (TAT→CAT)  dosP ← oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
RA 1,573,157 A→T I148N (ATC→AAC)  yddA ← ABC transporter ATP‑binding protein/permease
RA 1,575,825 A→T D319E (GAT→GAA ydeN ← sulfatase
RA 1,576,388 A→G S132P (TCC→CCC)  ydeN ← sulfatase
RA 1,579,109 A→C G545G (GGT→GGG ydeP ← acid resistance putative oxidoreductase YdeP
RA 1,613,753 (A)6→5 coding (377/435 nt) marR → multiple antibiotic resistance transcriptional regulator MarR
RA 1,632,437 (T)7→6 intergenic (‑71/+8) BW25113_RS08135 ← / ← BW25113_RS25620 hypothetical protein/hypothetical protein
RA 1,634,151 Δ1 bp coding (164/534 nt) rrrQ ← lysozyme
RA 1,649,476 A→G intergenic (‑78/+128) rspA ← / ← ynfA starvation‑sensing protein RspA/YnfA family protein
RA 1,659,576 T→C F14S (TTC→TCC)  clcB → voltage‑gated ClC‑type chloride channel ClcB
RA 1,671,899 T→G T88P (ACG→CCG)  pntA ← Re/Si‑specific NAD(P)(+) transhydrogenase subunit alpha
RA 1,673,542 A→G K287E (AAG→GAG)  ydgH → DUF1471 family protein YdgH
RA 1,717,498 G→C G374A (GGC→GCC)  ydhK → FUSC family protein
RA 1,782,276 T→G V192G (GTG→GGG)  ppsR → posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR
RA 1,803,201 A→G V97A (GTC→GCC)  yniB ← YniB family protein
RA 1,807,028 T→C E121G (GAA→GGA)  BW25113_RS09050 ← hypothetical protein
RA 1,812,807 (C)7→8 intergenic (‑50/+55) celF ← / ← chbR 6‑phospho‑beta‑glucosidase/transcriptional regulator ChbR
RA 1,838,813 T→C I106V (ATT→GTT)  ynjI ← DUF1266 domain‑containing protein
RA 1,854,126 T→C M121V (ATG→GTG)  ydjK ← MFS transporter
RA 1,877,816 T→C pseudogene (408/1482 nt) yeaV → BCCT family transporter YeaV
RA 1,897,230 A→G D309G (GAT→GGT)  manX → PTS mannose transporter subunit IIAB
RA 1,903,061 A→T intergenic (‑38/‑121) mgrB ← / → yobH PhoP/PhoQ regulator MgrB/YobH family protein
RA 1,934,547 T→C intergenic (‑97/+23) lpxM ← / ← mepM lauroyl‑Kdo(2)‑lipid IV(A) myristoyltransferase/murein DD‑endopeptidase MepM
RA 1,965,782 T→G E389D (GAA→GAC tar ← methyl‑accepting chemotaxis protein II
RA 1,980,420 A→G G5G (GGA→GGG ftnB → non‑heme ferritin‑like protein
RA 2,003,480 T→C F60S (TTC→TCC)  yedK → SOS response‑associated peptidase
RA 2,038,183 (T)5→4 intergenic (+78/‑236) BW25113_RS10330 → / → yeeJ tRNA‑Asn/inverse autotransporter adhesin YeeJ
RA 2,057,664 T→C G95G (GGA→GGG cobT ← nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
RA 2,064,259 T→G V93G (GTG→GGG)  BW25113_RS10440 → GTPase family protein
RA 2,102,646 T→C G140G (GGA→GGG wzx ← O16 family O‑antigen flippase
RA 2,131,004 A→G intergenic (‑280/‑379) wza ← / → yegH polysaccharide export protein Wza/TerC family protein
RA 2,174,381 T→G C270G (TGC→GGC)  yegU → ADP‑ribosylglycohydrolase family protein
RA 2,176,805 T→G D109A (GAC→GCC)  yegX ← glycoside hydrolase family 25 protein
RA 2,202,171 G→C W242C (TGG→TGC yehP → VWA domain‑containing protein
RA 2,220,044 A→G S236P (TCG→CCG)  yohF ← SDR family oxidoreductase
RA 2,229,956 A→G K405E (AAA→GAA)  preA → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
RA 2,283,874 Δ1 bp intergenic (‑350/‑105) BW25113_RS11435 ← / → narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
RA 2,284,519 T→C S181P (TCC→CCC)  narP → nitrate/nitrite response regulator protein NarP
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 2,410,868 A→T G103G (GGA→GGT yfcC → putative basic amino acid antiporter YfcC
RA 2,425,778 T→C E215G (GAA→GGA)  folC ← bifunctional tetrahydrofolate synthase/dihydrofolate synthase
RA 2,439,101 T→C K255E (AAG→GAG)  mepA ← penicillin‑insensitive murein endopeptidase
RA 2,505,777 T→C I137V (ATC→GTC)  mntH ← Nramp family divalent metal transporter
RA 2,526,050 A→G T95A (ACC→GCC)  cysK → cysteine synthase A
RA 2,536,106 T→C E261G (GAA→GGA)  cysP ← thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
RA 2,554,121 A→C N169T (AAC→ACC)  BW25113_RS12740 → hypothetical protein
RA 2,661,508 T→G T250P (ACC→CCC)  hcaR ← DNA‑binding transcriptional regulator HcaR
RA 2,678,723 A→C G48G (GGT→GGG glyA ← serine hydroxymethyltransferase
RA 2,726,562 (T)5→4 coding (971/2574 nt) clpB ← ATP‑dependent chaperone ClpB
RA 2,739,096 T→C E61G (GAA→GGA)  rimM ← ribosome maturation factor RimM
RA 2,751,237 A→G V241V (GTT→GTC BW25113_RS13685 ← hypothetical protein
RA 2,753,169 T→C S276P (TCA→CCA)  yfjI → YfjI family protein
RA 2,778,934 (T)6→5 intergenic (‑564/+187) BW25113_RS25705 ← / ← BW25113_RS13825 DUF5507 domain‑containing protein/tRNA‑Ile
RA 2,801,112 A→C T208P (ACC→CCC)  proX → glycine betaine/L‑proline ABC transporter substrate‑binding protein ProX
RA 2,814,267 A→G A368A (GCT→GCC alaS ← alanine‑‑tRNA ligase
RA 2,818,451 T→C E162G (GAA→GGA)  mltB ← lytic murein transglycosylase B
RA 2,819,095 T→C intergenic (‑160/‑96) mltB ← / → srlA lytic murein transglycosylase B/PTS glucitol/sorbitol transporter subunit IIC
RA 2,849,315 A→C Y540S (TAC→TCC)  flhA → formate hydrogenlyase transcriptional activator FlhA
RA 2,849,816 T→C E117G (GAA→GGA)  ygbA ← nitrous oxide‑stimulated promoter family protein
RA 2,859,998 T→C I305V (ATT→GTT)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,882,413 A→C V349G (GTT→GGT)  cysI ← assimilatory sulfite reductase (NADPH) hemoprotein subunit
RA 2,888,711 A→G V143A (GTC→GCC)  ygcQ ← electron transfer flavoprotein subunit alpha/FixB family protein
RA 2,982,555 T→C F232S (TTC→TCC)  yqeI → transcriptional regulator
RA 3,011,010 A→G E531G (GAA→GGA)  ygfK → putative selenate reductase subunit YgfK
RA 3,098,336 Δ1 bp coding (545/1080 nt) mltC → membrane‑bound lytic murein transglycosylase MltC
RA 3,103,871 (A)7→6 intergenic (+71/+78) BW25113_RS15410 → / ← yghD tRNA‑Phe/GspM family type II secretion system protein YghD
RA 3,273,721 A→G E49G (GAA→GGA)  agaV → PTS N‑acetylgalactosamine transporter subunit IIB
RA 3,458,386 A→G E369G (GAA→GGA)  gspL → type II secretion system protein GspL
RA 3,475,701 T→C S352P (TCG→CCG)  yheS → ABC transporter ATP‑binding protein
RA 3,535,339 T→C F525S (TTC→TCC)  feoB → Fe(2+) transporter permease subunit FeoB
RA 3,576,634 A→G R79R (CGA→CGG BW25113_RS17825 → YrhA family protein
RA 3,579,168 T→C E339G (GAA→GGA)  ggt ← gamma‑glutamyltransferase
RA 3,717,969 T→C E237G (GAA→GGA)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,786,467 T→C I193V (ATT→GTT)  yibB ← protein YibB
RA 3,786,991 T→C D18G (GAT→GGT)  yibB ← protein YibB
RA 3,791,182 A→C Y292S (TAC→TCC)  waaL → O‑antigen ligase
RA 3,798,416 (T)8→7 coding (61/936 nt) rfaS ← LPS core biosynthesis protein RfaS
RA 3,848,051 T→G F257C (TTT→TGT)  emrD → multidrug efflux MFS transporter EmrD
RA 3,852,078 T→G T467P (ACG→CCG)  yidK ← solute:sodium symporter family transporter
RA 3,896,621 A→G D147D (GAT→GAC bglB ← 6‑phospho‑beta‑glucosidase BglB
RA 3,898,828 (T)5→4 coding (130/1878 nt) bglF ← PTS beta‑glucoside transporter subunit IIABC
RA 4,034,572 T→G Q74H (CAA→CAC mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein MobB
RA 4,073,163 A→T D26V (GAT→GTT)  BW25113_RS20185 → hypothetical protein
RA 4,084,653 A→G G358G (GGT→GGC frvB ← PTS fructose‑like transporter subunit IIBC
RA 4,117,071 T→C F44S (TTC→TCC)  rpmE → 50S ribosomal protein L31
RA 4,153,816 T→C S84P (TCA→CCA)  btuB → TonB‑dependent vitamin B12 receptor BtuB
RA 4,180,586 (A)7→6 intergenic (+333/+77) yjaZ → / ← thiH heat‑shock protein C/2‑iminoacetate synthase ThiH
RA 4,211,170 (T)8→7 coding (1246/2187 nt) espL4 ← type III secretion system effector EspL4
RA 4,215,724 T→G W657G (TGG→GGG)  metH → methionine synthase
RA 4,306,692 A→T I115N (ATC→AAC)  phnM ← alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase
RA 4,367,563 T→C intergenic (‑24/+65) blc ← / ← ampC lipocalin Blc/BlaEC family class C beta‑lactamase
RA 4,494,539 T→C F222S (TTC→TCC)  nanX → MFS transporter
RA 4,527,151 T→C Q87Q (CAA→CAG nanS ← 9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
RA 4,543,103 A→G E129G (GAA→GGA)  uxuB → fructuronate reductase
RA 4,593,447 T→C S52P (TCC→CCC)  yjjQ → response regulator transcription factor
RA 4,613,222 C→G R238P (CGC→CCC)  lplA ← lipoate‑‑protein ligase LplA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 279522 279599 78 2 [1] [1] 2 yagF xylonate dehydratase YagF
* * ÷ NZ_CP009273 356951 357569 619 2 [1] [1] 2 [lacA] [lacA]
* * ÷ NZ_CP009273 428545 428562 18 2 [1] [1] 2 nrdR transcriptional regulator NrdR
* * ÷ NZ_CP009273 463322 463357 36 2 [1] [1] 2 cof HMP‑PP phosphatase
* * ÷ NZ_CP009273 510218 510229 12 4 [0] [1] 2 ybbJ NfeD family protein
* * ÷ NZ_CP009273 510320 510325 6 2 [1] [1] 2 qmcA SPFH/Band 7/PHB domain protein
* * ÷ NZ_CP009273 531703 531707 5 2 [0] [0] 2 hyi hydroxypyruvate isomerase
* * ÷ NZ_CP009273 546184 546196 13 2 [1] [1] 2 ybcF carbamate kinase
* * ÷ NZ_CP009273 616996 617018 23 2 [1] [1] 2 fepD Fe(3+)‑siderophore ABC transporter permease
* * ÷ NZ_CP009273 703091 703171 81 2 [1] [1] 2 glnS glutamine‑‑tRNA ligase
* * ÷ NZ_CP009273 712095 712153 59 2 [1] [1] 2 BW25113_RS03565/BW25113_RS03570 DUF2625 domain‑containing protein/winged helix‑turn‑helix domain‑containing protein
* * ÷ NZ_CP009273 716337 716439 103 2 [0] [1] 3 BW25113_RS26090/kdpE leader peptide SpeFL/two‑component system response regulator KdpE
* * ÷ NZ_CP009273 719342 719386 45 2 [1] [1] 3 kdpD two‑component system sensor histidine kinase KdpD
* * ÷ NZ_CP009273 763041 763089 49 2 [1] [1] 2 mngA PTS 2‑O‑a‑mannosyl‑D‑glycerate transporter subunit IIABC
* * ÷ NZ_CP009273 764355 764403 49 2 [1] [0] 2 mngB mannosylglycerate hydrolase
* * ÷ NZ_CP009273 766309 766392 84 2 [1] [1] 2 mngB/cydA mannosylglycerate hydrolase/cytochrome ubiquinol oxidase subunit I
* * ÷ NZ_CP009273 772496 772517 22 2 [0] [1] 2 tolA cell envelope integrity protein TolA
* * ÷ NZ_CP009273 779847 779876 30 3 [1] [1] 2 zitB CDF family zinc transporter ZitB
* * ÷ NZ_CP009273 804746 804763 18 2 [1] [1] 3 bioA/bioB adenosylmethionine‑‑8‑amino‑7‑oxononanoate transaminase/biotin synthase BioB
* * ÷ NZ_CP009273 808696 808702 7 2 [1] [1] 2 bioD/uvrB dethiobiotin synthase/excinuclease ABC subunit UvrB
* * ÷ NZ_CP009273 828565 828622 58 2 [1] [1] 2 dinG ATP‑dependent DNA helicase DinG
* * ÷ NZ_CP009273 843217 843225 9 5 [1] [1] 2 glnH glutamine ABC transporter substrate‑binding protein GlnH
* * ÷ NZ_CP009273 861492 861538 47 3 [1] [1] 2 moeA molybdopterin molybdotransferase MoeA
* * ÷ NZ_CP009273 862476 862483 8 3 [1] [1] 2 iaaA beta‑aspartyl‑peptidase
* * ÷ NZ_CP009273 897880 897892 13 3 [1] [1] 2 artJ arginine ABC transporter substrate‑binding protein
* * ÷ NZ_CP009273 913363 913390 28 2 [1] [1] 3 ybjD ATP‑dependent endonuclease
* * ÷ NZ_CP009273 916728 916766 39 2 [1] [1] 2 macB macrolide ABC transporter ATP‑binding protein/permease MacB
* * ÷ NZ_CP009273 927701 927719 19 2 [0] [1] 4 trxB/lrp thioredoxin‑disulfide reductase/leucine‑responsive transcriptional regulator Lrp
* * ÷ NZ_CP009273 950053 950075 23 3 [1] [1] 2 focA/ycaO formate transporter FocA/30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
* * ÷ NZ_CP009273 950240 950285 46 2 [1] [1] 2 focA/ycaO formate transporter FocA/30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
* * ÷ NZ_CP009273 971747 971771 25 2 [1] [1] 2 mukE chromosome partition protein MukE
* * ÷ NZ_CP009273 990237 990291 55 2 [1] [1] 2 [ssuC] [ssuC]
* * ÷ NZ_CP009273 1010950 1011000 51 2 [1] [1] 2 pqiC/rmf membrane integrity‑associated transporter subunit PqiC/ribosome modulation factor
* * ÷ NZ_CP009273 1046819 1046863 45 2 [1] [1] 2 cspH/cspG cold shock‑like protein CspH/cold shock protein CspG
* * ÷ NZ_CP009273 1066822 1066830 9 2 [0] [1] 2 rutD pyrimidine utilization protein D
* * ÷ NZ_CP009273 1071901 1071937 37 3 [1] [1] 2 putA trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
* * ÷ NZ_CP009273 1085152 1085182 31 3 [1] [1] 2 pgaB poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase PgaB
* * ÷ NZ_CP009273 1086794 1086823 30 3 [1] [1] 2 pgaA poly‑beta‑1,6 N‑acetyl‑D‑glucosamine export porin PgaA
* * ÷ NZ_CP009273 1101112 1101113 2 2 [0] [0] 2 ymdA hypothetical protein
* * ÷ NZ_CP009273 1107275 1107307 33 2 [0] [1] 2 mdoH glucans biosynthesis glucosyltransferase MdoH
* * ÷ NZ_CP009273 1118153 1118243 91 2 [1] [1] 2 [yceB] [yceB]
* * ÷ NZ_CP009273 1121824 1121830 7 2 [1] [0] 2 yceH YceH family protein
* * ÷ NZ_CP009273 1122286 1122370 85 2 [1] [1] 2 yceM Gfo/Idh/MocA family oxidoreductase
* * ÷ NZ_CP009273 1124020 1124034 15 2 [1] [1] 2 murJ murein biosynthesis integral membrane protein MurJ
* * ÷ NZ_CP009273 1145634 1145680 47 2 [1] [1] 2 fabD ACP S‑malonyltransferase
* * ÷ NZ_CP009273 1152701 1152726 26 2 [1] [1] 2 ycfH metal‑dependent hydrolase
* * ÷ NZ_CP009273 1158155 1158236 82 2 [1] [1] 2 lpoB penicillin‑binding protein activator LpoB
* * ÷ NZ_CP009273 1159743 1159779 37 2 [1] [0] 2 nagZ beta‑N‑acetylhexosaminidase
* * ÷ NZ_CP009273 1165713 1165745 33 2 [1] [0] 3 ldtC L,D‑transpeptidase LdtC
* * ÷ NZ_CP009273 1167420 1167425 6 2 [0] [0] 2 mfd transcription‑repair coupling factor
* * ÷ NZ_CP009273 1172668 1172701 34 2 [1] [1] 2 lolD lipoprotein‑releasing ABC transporter ATP‑binding protein LolD
* * ÷ NZ_CP009273 1176269 1176276 8 2 [0] [1] 2 ycfZ TPM domain‑containing protein
* * ÷ NZ_CP009273 1211984 1212158 175 3 [1] [1] 2 [ariR] [ariR]
* * ÷ NZ_CP009273 1225985 1226010 26 2 [1] [1] 3 hlyE/umuD hemolysin HlyE/translesion error‑prone DNA polymerase V autoproteolytic subunit
* * ÷ NZ_CP009273 1236310 1236339 30 2 [1] [1] 2 cvrA potassium/proton antiporter
* * ÷ NZ_CP009273 1239685 1239722 38 2 [1] [1] 2 ycgR flagellar brake protein
* * ÷ NZ_CP009273 1256076 1256078 3 3 [0] [0] 3 dauA C4‑dicarboxylic acid transporter DauA
* * ÷ NZ_CP009273 1262930 1262968 39 7 [1] [1] 2 sirB1 invasion regulator SirB1
* * ÷ NZ_CP009273 1267316 1267361 46 2 [1] [0] 2 chaA/chaB sodium‑potassium/proton antiporter ChaA/putative cation transport regulator ChaB
* * ÷ NZ_CP009273 1284909 1284974 66 2 [1] [1] 2 rssA patatin‑like phospholipase RssA
* * ÷ NZ_CP009273 1302590 1302644 55 2 [1] [1] 2 cls cardiolipin synthase
* * ÷ NZ_CP009273 1314452 1314480 29 2 [1] [1] 2 trpD bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
* * ÷ NZ_CP009273 1316332 1316364 33 2 [0] [1] 2 trpE anthranilate synthase component I
* * ÷ NZ_CP009273 1319047 1319114 68 3 [1] [1] 2 yciQ DUF2207 domain‑containing protein
* * ÷ NZ_CP009273 1319249 1319277 29 2 [0] [1] 2 yciQ DUF2207 domain‑containing protein
* * ÷ NZ_CP009273 1324803 1324819 17 3 [0] [0] 2 yciN YciN family protein
* * ÷ NZ_CP009273 1327585 1327646 62 2 [1] [1] 3 topA type I DNA topoisomerase
* * ÷ NZ_CP009273 1342848 1342857 10 2 [0] [0] 2 rnb exoribonuclease II
* * ÷ NZ_CP009273 1344920 1344927 8 2 [1] [0] 2 fabI enoyl‑ACP reductase FabI
* * ÷ NZ_CP009273 1360228 1360238 11 3 [0] [1] 2 puuE 4‑aminobutyrate transaminase
* * ÷ NZ_CP009273 1362545 1362581 37 2 [1] [0] 2 pspA phage shock protein PspA
* * ÷ NZ_CP009273 1372900 1372963 64 2 [1] [0] 2 ycjT kojibiose phosphorylase
* * ÷ NZ_CP009273 1387250 1387322 73 2 [1] [1] 3 pgrR/mppA DNA‑binding transcriptional regulator PgrR/murein tripeptide ABC transporter substrate‑binding protein MppA
* * ÷ NZ_CP009273 1424195 1424249 55 2 [1] [0] 2 stfR prophage tail fiber N‑terminal domain‑containing protein
* * ÷ NZ_CP009273 1427985–1428244 1428284 41–300 2 [1] [1] 2 [ydfK] [ydfK]
* * ÷ NZ_CP009273 1431601 1431608 8 4 [0] [0] 2 nifJ pyruvate:ferredoxin (flavodoxin) oxidoreductase
* * ÷ NZ_CP009273 1437540 1437566 27 2 [1] [1] 2 ydbH YdbH family protein
* * ÷ NZ_CP009273 1485096 1485165 70 2 [1] [1] 2 [cybB] [cybB]
* * ÷ NZ_CP009273 1538575 1538591 17 3 [1] [1] 2 narU/BW25113_RS07710 nitrate/nitrite transporter NarU/leucine‑rich repeat domain‑containing protein
* * ÷ NZ_CP009273 1627556 1627848–1627566 11–293 2 [1] [1] 3 [ydfK] [ydfK]
* * ÷ NZ_CP009273 1663696 1663788 93 2 [1] [1] 2 ynfL LysR family transcriptional regulator
* * ÷ NZ_CP009273 1703422 1703432 11 3 [0] [0] 2 rsxD electron transport complex subunit RsxD
* * ÷ NZ_CP009273 1887490 1887505 16 2 [1] [1] 2 [yoaA] [yoaA]
* * ÷ NZ_CP009273 1922581 1922591 11 2 [1] [1] 2 ptrB oligopeptidase B
* * ÷ NZ_CP009273 1973016 1973043 28 2 [1] [1] 2 flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* * ÷ NZ_CP009273 1981381 1981475 95 4 [1] [1] 2 [azuC] [azuC]
* * ÷ NZ_CP009273 1983056 1983080 25 2 [1] [1] 2 yecH/tyrP YecH family protein/tyrosine transporter TyrP
* * ÷ NZ_CP009273 2033088 2033211 124 3 [1] [1] 2 msrP protein‑methionine‑sulfoxide reductase catalytic subunit MsrP
* * ÷ NZ_CP009273 2042367 2042388 22 2 [1] [0] 2 yeeJ inverse autotransporter adhesin YeeJ
* * ÷ NZ_CP009273 2051390 2051432 43 2 [1] [1] 2 yeeN/BW25113_RS10360 YebC/PmpR family DNA‑binding transcriptional regulator/tRNA‑Asn
* * ÷ NZ_CP009273 2075175 2075187 13 2 [1] [0] 2 dacD serine‑type D‑Ala‑D‑Ala carboxypeptidase DacD
* * ÷ NZ_CP009273 2144447 2144494 48 2 [1] [1] 2 [yegI] [yegI]
* * ÷ NZ_CP009273 2174603 2174628 26 2 [1] [1] 2 yegV carbohydrate kinase family protein
* * ÷ NZ_CP009273 2272164 2272185 22 2 [1] [1] 2 bcr multidrug efflux MFS transporter Bcr
* * ÷ NZ_CP009273 2295679 2295687 9 2 [1] [1] 2 napA periplasmic nitrate reductase subunit alpha
* * ÷ NZ_CP009273 2321403 2321469 67 3 [1] [1] 2 yfaP YfaP family protein
* * ÷ NZ_CP009273 2341486 2341510 25 2 [1] [1] 2 nrdB ribonucleotide‑diphosphate reductase subunit beta
* * ÷ NZ_CP009273 2399959 2399979 21 2 [1] [1] 2 lrhA transcriptional regulator LrhA
* * ÷ NZ_CP009273 2436273 2436308 36 2 [1] [1] 2 mnmC bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC
* * ÷ NZ_CP009273 2491984 2492014 31 2 [1] [1] 2 alaC/ypdA alanine transaminase/two‑component system sensor histidine kinase YpdA
* * ÷ NZ_CP009273 2505218 2505318 101 2 [1] [1] 2 mntH Nramp family divalent metal transporter
* * ÷ NZ_CP009273 2512943 2512978 36 2 [1] [1] 2 gltX glutamate‑‑tRNA ligase
* * ÷ NZ_CP009273 2543418 2543475 58 2 [1] [1] 2 yfeX porphyrinogen peroxidase
* * ÷ NZ_CP009273 2563799 2563838 40 2 [1] [0] 2 eutE aldehyde dehydrogenase EutE
* * ÷ NZ_CP009273 2588549 2588588 40 2 [1] [1] 2 tmcA tRNA cytosine(34) acetyltransferase TmcA
* * ÷ NZ_CP009273 2604910 2604920 11 2 [1] [1] 2 hyfJ hydrogenase 4 assembly chaperone HyfJ
* * ÷ NZ_CP009273 2609088 2609118 31 2 [1] [1] 2 yfgO AI‑2E family transporter
* * ÷ NZ_CP009273 2630860 2630880 21 3 [1] [1] 2 bamB outer membrane protein assembly factor BamB
* * ÷ NZ_CP009273 2638503 2638519 17 2 [1] [1] 4 pbpC peptidoglycan glycosyltransferase PbpC
* * ÷ NZ_CP009273 2651466 2651524 59 2 [1] [1] 2 hscA Fe‑S protein assembly chaperone HscA
* * ÷ NZ_CP009273 2674119 2674141 23 2 [0] [1] 2 yphG DUF5107 domain‑containing protein
* * ÷ NZ_CP009273 2687958 2688020 63 2 [1] [1] 2 purL phosphoribosylformylglycinamidine synthase
* * ÷ NZ_CP009273 2707461 2707521 61 2 [1] [1] 2 srmB ATP‑dependent RNA helicase SrmB
* * ÷ NZ_CP009273 2961030 2961062 33 2 [1] [1] 2 ptsP phosphoenolpyruvate‑‑protein phosphotransferase
* * ÷ NZ_CP009273 3087953 3087968 16 3 [1] [1] 2 yggR type IV pilus twitching motility protein PilT
* * ÷ NZ_CP009273 3286002 3286016 15 2 [1] [1] 2 rsmI 16S rRNA (cytidine(1402)‑2'‑O)‑methyltransferase
* * ÷ NZ_CP009273 3819724 3819738 15 2 [1] [1] 2 recG ATP‑dependent DNA helicase RecG
* * ÷ NZ_CP009273 4205273 4205334 62 2 [1] [1] 2 metA/aceB homoserine O‑succinyltransferase/malate synthase A
* * ÷ NZ_CP009273 4316830 4316859 30 4 [1] [0] 2 yjdM/crfC zinc ribbon domain‑containing protein YjdM/clamp‑binding protein CrfC

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)13 (1.050) 13/170 0.1 52.0% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 12 (0.970)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =10 (0.810)4 (0.400) 4/138 0.7 44.2% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 2 (0.200)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)11 (0.900) 9/168 0.3 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)19 (1.530) 13/170 0.1 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP