breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,811,215 Δ1 bp coding (446/531 nt) mprA → transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 3,413,571 T→C intergenic (‑106/‑293) acrS ← / → acrE acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,580,806 Δ2,622 bp IS1‑mediated ryhByrhA ryhB, yhhY, yhhZ, yrhA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2995175 2995271 97 5 [4] [4] 5 ygeK/ygeN pseudogene, response regulator family, part of T3SS PAI ETT2 remnant; putative 2‑component transcriptional regulator/pseudogene, orgB family, part of T3SS PAI ETT2 remnant
* * ÷ NC_000913 3423723–3424234 3424599–3424238 5–877 5 [3] [4] 7 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)27 (0.940) 21/124 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 2077808 =31 (1.010)10 (0.400) 4/108 2.1 21.0% coding (240/375 nt) cbtA CP4‑44 prophage; toxin of the YeeV‑YeeU toxin‑antitoxin system
?NC_000913 2077982 = 50 (2.000)pseudogene (43/168 nt) yeeW CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related
* ? NC_000913 2158238 =13 (0.420)4 (0.140) 3/122 2.7 25.8% coding (2976/3123 nt) mdtB multidrug efflux system, subunit B
?NC_000913 2158297 = 11 (0.390)coding (3035/3123 nt) mdtB multidrug efflux system, subunit B
* ? NC_000913 3620750 =NA (NA)4 (0.130) 3/128 2.8 100% coding (1559/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3621165 = 0 (0.000)coding (1974/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 3704467 =29 (0.950)7 (0.260) 3/116 2.6 20.2% coding (295/903 nt) dppC dipeptide/heme ABC transporter permease
?NC_000913 3704731 = 30 (1.120)coding (31/903 nt) dppC dipeptide/heme ABC transporter permease
* ? NC_000913 = 383104417 (0.550)8 (0.310) 3/110 2.5 38.0% coding (588/1710 nt) yicH putative inner membrane‑anchored periplasmic AsmA family protein
?NC_000913 = 3831092 12 (0.470)coding (636/1710 nt) yicH putative inner membrane‑anchored periplasmic AsmA family protein
* ? NC_000913 3927418 =23 (0.750)5 (0.200) 3/108 2.4 24.5% coding (264/993 nt) asnA asparagine synthetase A
?NC_000913 3927457 = 12 (0.480)coding (303/993 nt) asnA asparagine synthetase A
* ? NC_000913 4164667 =34 (1.110)7 (0.250) 3/122 2.7 21.7% coding (1029/1845 nt) btuB vitamin B12/cobalamin outer membrane transporter
?NC_000913 4164716 = 19 (0.670)coding (1078/1845 nt) btuB vitamin B12/cobalamin outer membrane transporter
* ? NC_000913 = 439240211 (0.360)3 (0.110) 3/116 2.6 22.5% noncoding (43/76 nt) glyV tRNA‑Gly
?NC_000913 = 4392497 11 (0.410)noncoding (26/76 nt) glyX tRNA‑Gly
* ? NC_000913 4542682 =7 (0.230)6 (0.230) 5/114 1.9 34.3% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 17 (0.640)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 = 45426907 (0.230)3 (0.110) 3/114 2.5 20.7% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 17 (0.640)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)