breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
JC JC 486,122 IS1 (–) +8 bp coding (362‑369/648 nt) acrR → transcriptional repressor
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,811,095 A→C D109A (GAT→GCT)  mprA → transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 3,413,571 T→C intergenic (‑106/‑293) acrS ← / → acrE acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1630446 1630497 52 3 [1] [1] 2 ydfI putative NAD‑dependent D‑mannonate oxidoreductase
* * ÷ NC_000913 2374795 2374874 80 2 [1] [1] 3 menE O‑succinylbenzoate‑CoA ligase
* * ÷ NC_000913 2572549 2572553 5 3 [1] [0] 2 eutD phosphate acetyltransferase
* * ÷ NC_000913 3162355 3162364 10 2 [1] [0] 2 ftsP septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)
* * ÷ NC_000913 3423723–3424234 3424593–3424238 5–871 2 [1] [1] 2 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3849615 3849638 24 2 [0] [1] 3 uhpB sensory histidine kinase in two‑component regulatory sytem with UhpA
* * ÷ NC_000913 4235750 4235760 11 2 [1] [1] 2 pgi/yjbE glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein
* * ÷ NC_000913 4296113–4296378 4296392 15–280 2 [1] [1] 2 gltP/yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 224412 =NA (NA)3 (0.180) 3/118 1.6 NA noncoding (642/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 224936 = NA (NA)noncoding (1166/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 = 291401NA (NA)33 (1.780) 19/132 0.1 62.3% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 807574 20 (1.080)coding (336/477 nt) ybhB kinase inhibitor homolog, UPF0098 family
* ? NC_000913 315401 =NA (NA)4 (0.270) 4/106 1.1 NA noncoding (173/1255 nt) IS3 repeat region
?NC_000913 315627 = NA (NA)noncoding (399/1255 nt) IS3 repeat region
* ? NC_000913 = 35548617 (0.920)3 (0.180) 3/118 1.6 21.3% coding (565/1260 nt) codB cytosine transporter
?NC_000913 = 355537 7 (0.420)coding (616/1260 nt) codB cytosine transporter
* ? NC_000913 771005 =9 (0.480)27 (1.450) 19/132 0.1 75.0% intergenic (+394/‑453) mngB/cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
?NC_000913 = 1050545 NA (NA)noncoding (768/768 nt) IS1 repeat region
* ? NC_000913 = 12994980 (0.000)30 (1.720) 22/124 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1492172 =10 (0.540)3 (0.180) 3/118 1.6 27.4% noncoding (54/56 nt) sokB sRNA antisense regulator blocking mokB, and hence hokB, translation
?NC_000913 1492206 = 7 (0.420)intergenic (+32/‑264) sokB/trg sRNA antisense regulator blocking mokB, and hence hokB, translation/methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
* ? NC_000913 = 16364075 (0.270)4 (0.290) 3/100 1.3 42.6% intergenic (‑40/‑349) nohQ/ynfO pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage
?NC_000913 = 1636432 7 (0.500)intergenic (‑65/‑324) nohQ/ynfO pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage
* ? NC_000913 = 323466010 (0.660)4 (0.260) 3/108 1.4 28.6% noncoding (23/86 nt) REP233 (repetitive extragenic palindromic) element; contains 2 REP sequences REP233 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913 = 3234703 NA (NA)noncoding (66/86 nt) REP233 (repetitive extragenic palindromic) element; contains 2 REP sequences REP233 (repetitive extragenic palindromic) element; contains 2 REP sequences
* ? NC_000913 = 335560612 (0.650)5 (0.310) 4/116 1.3 24.7% coding (882/4461 nt) gltB glutamate synthase, large subunit
?NC_000913 = 3355642 20 (1.230)coding (918/4461 nt) gltB glutamate synthase, large subunit
* ? NC_000913 3927418 =17 (0.920)6 (0.400) 4/108 1.2 29.4% coding (264/993 nt) asnA asparagine synthetase A
?NC_000913 3927457 = 15 (0.990)coding (303/993 nt) asnA asparagine synthetase A