breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,312,212 +TC coding (538/1104 nt) ompC ← outer membrane porin protein C
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 3,413,571 T→C intergenic (‑106/‑293) acrS ← / → acrE acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,114,469 C→A A2S (GCT→TCT)  fpr ← ferredoxin‑NADP reductase; flavodoxin reductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1757086 1757430 345 76 [0] [0] 38 [pykF]–[lpp] [pykF],[lpp]
* * ÷ NC_000913 3423709–3424597 3424597 1–889 10 [6] [8] 11 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3875506 3875541 36 9 [8] [8] 9 yidX putative lipoprotein
* * ÷ NC_000913 4296108–4296379 4296385 7–278 9 [8] [8] 9 gltP/yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 24300936 (0.930)9 (0.260) 6/116 2.3 20.2% coding (295/2445 nt) fadE acyl coenzyme A dehydrogenase
?NC_000913 = 243192 39 (1.130)coding (112/2445 nt) fadE acyl coenzyme A dehydrogenase
* ? NC_000913 315401 =NA (NA)15 (0.480) 7/104 1.8 NA noncoding (173/1255 nt) IS3 repeat region
?NC_000913 315627 = NA (NA)noncoding (399/1255 nt) IS3 repeat region
* ? NC_000913 = 55583332 (0.830)6 (0.210) 5/98 2.2 21.4% coding (1223/1386 nt) cysS cysteinyl‑tRNA synthetase
?NC_000913 = 556784 20 (0.680)coding (90/213 nt) ybcJ ribosome‑associated protein; putative RNA‑binding protein
* ? NC_000913 1207790 =4 (0.100)19 (0.650) 12/98 0.7 88.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 2 (0.070)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078054 (0.100)18 (0.620) 12/98 0.7 87.8% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 2 (0.070)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1287474 =24 (0.620)9 (0.260) 7/114 2.0 29.9% intergenic (‑146/+64) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
?NC_000913 1287554 = NA (NA)noncoding (69/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 17570850 (0.000)76 (1.960) 28/130 0.1 100% coding (1388/1413 nt) pykF pyruvate kinase I
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 1757431 =0 (0.000)37 (0.950) 16/130 0.8 100% coding (11/237 nt) lpp murein lipoprotein
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 2277401 =34 (0.880)7 (0.210) 5/110 2.5 20.4% coding (1102/1590 nt) yejF microcin C ABC transporter ATPase
?NC_000913 2277552 = 26 (0.790)coding (1253/1590 nt) yejF microcin C ABC transporter ATPase
* ? NC_000913 2803441 =57 (1.470)14 (0.430) 4/110 2.9 23.9% coding (2094/2145 nt) nrdE ribonucleoside‑diphosphate reductase 2, alpha subunit
?NC_000913 2803529 = 41 (1.250)coding (28/960 nt) nrdF ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein
* ? NC_000913 = 379832533 (0.850)26 (0.700) 6/124 2.5 35.0% coding (988/1074 nt) waaU lipopolysaccharide core biosynthesis
?NC_000913 = 3798516 65 (1.760)coding (797/1074 nt) waaU lipopolysaccharide core biosynthesis
* ? NC_000913 3927418 =19 (0.490)7 (0.220) 5/106 2.4 30.1% coding (264/993 nt) asnA asparagine synthetase A
?NC_000913 3927457 = 17 (0.540)coding (303/993 nt) asnA asparagine synthetase A
* ? NC_000913 = 392742919 (0.490)5 (0.160) 4/106 2.8 23.5% coding (275/993 nt) asnA asparagine synthetase A
?NC_000913 = 3927444 17 (0.540)coding (290/993 nt) asnA asparagine synthetase A