breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 353,610 C→G 18.7% A302G (GCG→GGG)  prpE → propionate‑‑CoA ligase
RA 357,248 Δ1 bp 2.6% coding (1078/1284 nt) codA → cytosine/isoguanine deaminase
RA 374,944 G→A 4.3% intergenic (+63/‑515) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 385,818 T→G 13.8% V196G (GTC→GGC)  tauA → taurine ABC transporter periplasmic binding protein
RA 386,999 A→G 3.5% H10R (CAT→CGT)  tauC → taurine ABC transporter permease
RA 422,231 A→T 13.7% I416F (ATC→TTC)  proY → proline‑specific permease
RA 433,649 T→G 27.1% G65G (GGT→GGG ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
RA 452,427 A→C 16.1% G373G (GGT→GGG ampG ← muropeptide transporter
RA 599,239 Δ1 bp 2.6% coding (526/3144 nt) cusA → copper/silver efflux system, membrane component
RA 599,612 T→G 14.2% V300G (GTG→GGG)  cusA → copper/silver efflux system, membrane component
RA 722,927 A→T 3.4% A496A (GCT→GCA kdpD ← fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein
RA 825,230 C→A 16.3% M293I (ATG→ATT ybhR ← putative ABC transporter permease
RA 836,592 T→G 16.5% V81G (GTC→GGC)  ybiC → putative dehydrogenase
RA 836,625 T→G 14.7% V92G (GTC→GGC)  ybiC → putative dehydrogenase
RA 859,732 A→T 2.8% D99E (GAT→GAA ybiV ← sugar phosphatase, fructose‑1‑P/ribose‑5‑P/glucose‑6‑P phosphatase
RA 954,374 C→G 14.2% P31P (CCG→CCC focA ← formate channel
RA 976,132 Δ1 bp 2.8% coding (511/705 nt) mukE → chromosome condensin MukBEF, MukE localization factor
RA 989,943 T→G 22.4% S138S (TCA→TCC pncB ← nicotinate phosphoribosyltransferase
RA 989,959 A→C 17.6% V133G (GTA→GGA)  pncB ← nicotinate phosphoribosyltransferase
RA 1,078,659 T→G 11.8% H75P (CAC→CCC)  putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
RA 1,143,829 A→C 94.5% L180R (CTG→CGG)  rne ← endoribonuclease, RNA‑binding protein,RNA degradosome binding protein
RA 1,196,305 C→T 100% R395C (CGT→TGT)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
RA 1,358,812 A→G 20.6% S126P (TCC→CCC)  puuP ← putrescine importer
RA 1,360,585 A→G 9.3% T108T (ACT→ACC puuA ← glutamate‑‑putrescine ligase
RA 1,451,640 Δ1 bp 3.4% coding (2003/2046 nt) paaZ ← oxepin‑CoA hydrolase and 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,466,210 G→A 4.2% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,611,919 C→A 16.2% intergenic (‑65/+47) yneF ← / ← yneG putative membrane‑bound diguanylate cyclase/DUF4186 family protein
RA 1,706,082 T→G 8.5% F106V (TTT→GTT)  rsxA → SoxR iron‑sulfur cluster reduction factor component, inner membrane protein of electron transport complex
RA 1,708,930 C→A 12.5% P671Q (CCG→CAG)  rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 2,043,021 Δ1 bp 8.3% intergenic (+123/+447) yodB → / ← serU cytochrome b561 homolog/tRNA‑Ser
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,151,689 G→T 30.3% intergenic (+43/+22) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein
RA 2,228,308 G→C 14.4% pseudogene (300/1287 nt) mdtQ ← pseudogene, putative channel/filament proteins
RA 2,304,520 G→T 5.8% intergenic (+129/+586) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,521 G→C 5.7% intergenic (+130/+585) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,335,096 G→A 3.5% P516L (CCG→CTG)  yfaA ← DUF2138 family protein, putative host defense protein
RA 2,378,967 T→C 16.1% G97G (GGA→GGG menD ← 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase, SEPHCHC synthase
RA 2,383,621 A→T 25.0% D304V (GAT→GTT)  elaD → protease, capable of cleaving an AMC‑ubiquitin model substrate
RA 2,434,880 T→G 25.8% Y252S (TAC→TCC)  truA ← tRNA pseudouridine(38‑40) synthase
RA 2,445,138:1 +C 4.4% coding (421/810 nt) yfcA ← TauE/TSUP family inner membrane protein
RA 2,608,229 A→G 14.9% M439V (ATG→GTG)  hyfF → hydrogenase 4, membrane subunit
RA 2,608,251 C→G 9.8% A446G (GCG→GGG)  hyfF → hydrogenase 4, membrane subunit
RA 2,818,451 C→T 5.1% intergenic (‑179/+20) argY ← / ← argV tRNA‑Arg/tRNA‑Arg
RA 2,828,504 T→G 20.4% V257G (GTG→GGG)  srlD → sorbitol‑6‑phosphate dehydrogenase
RA 2,833,565 T→G 17.5% G364G (GGT→GGG norV → anaerobic nitric oxide reductase flavorubredoxin
RA 3,205,084 A→T 9.3% L131F (TTA→TTT plsY → putative glycerol‑3‑phosphate acyltransferase
RA 3,206,436 G→A 18.0% intergenic (‑182/‑25) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,206,454 A→G 10.3% intergenic (‑200/‑7) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,206,469 C→G 18.3% S3R (AGC→AGG ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,474 G→A 12.0% S5N (AGT→AAT) ‡ ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,475 T→A 21.3% S5R (AGT→AGA) ‡ ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,481 G→A 30.5% K7K (AAG→AAA ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,482 C→A 18.6% Q8K (CAA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,485 C→A 19.9% Q9K (CAG→AAG)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,492 T→A 10.7% V11E (GTG→GAG)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,504 C→A 9.5% T15K (ACA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,298,058 Δ1 bp 3.1% coding (79/1041 nt) yraQ ← putative inner membrane permease
RA 3,436,119 T→C 3.1% R305R (CGT→CGC rsmB → 16S rRNA m(5)C967 methyltransferase, SAM‑dependent
RA 3,468,600 T→C 3.5% S418G (AGC→GGC)  chiA ← periplasmic endochitinase
RA 3,520,794 G→T 9.1% T75K (ACA→AAA)  hofP ← DNA catabolic protein
RA 3,571,847:1 +G 3.0% coding (1655/2187 nt) glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,617,326 A→C 16.2% T105P (ACC→CCC)  nikD → nickel ABC transporter ATPase
RA 3,665,701 C→A 100% A37S (GCG→TCG)  gadX ← acid resistance regulon transcriptional activator, autoactivator
RA 3,760,383 A→C 22.5% V289G (GTG→GGG)  selA ← selenocysteine synthase
RA 3,782,907 T→G 27.1% T252P (ACC→CCC)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)
RA 3,782,912 A→C 15.3% V250G (GTG→GGG)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)
RA 3,786,532 T→G 15.6% V425G (GTG→GGG)  gpmM → phosphoglycero mutase III, cofactor‑independent
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,862,669 T→A 28.3% pseudogene (934/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
RA 3,864,958 T→C 18.4% I439V (ATC→GTC)  yidE ← putative transporter
RA 3,904,282 T→G 11.1% I439L (ATC→CTC)  bglF ← fused beta‑glucoside‑specific PTS enzymes: IIA component/IIB component/IIC component
JC 4,001,644 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,027,131 A→G 11.1% G202G (GGA→GGG fre → NAD(P)H‑flavin reductase
RA 4,027,145 C→G 17.8% A207G (GCG→GGG)  fre → NAD(P)H‑flavin reductase
RA 4,135,825 Δ1 bp 2.6% coding (1992/2181 nt) katG → catalase‑peroxidase HPI, heme b‑containing
RA 4,151,328 T→G 28.1% A590A (GCA→GCC ppc ← phosphoenolpyruvate carboxylase
RA 4,162,525 G→T 9.5% Q249K (CAA→AAA)  trmA ← tRNA m(5)U54 methyltransferase, SAM‑dependent, tmRNA m(5)U341 methyltransferase
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,237,260 T→C 21.0% V122A (GTC→GCC)  yjbG → extracellular polysaccharide export OMA protein
RA 4,296,059 C→T 5.7% intergenic (+266/+378) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,267 T→C 8.4% intergenic (+474/+170) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,325,949 A→G 5.1% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,345,640 G→A 4.0% intergenic (‑81/+41) yjdF ← / ← fumB DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
RA 4,345,641 A→T 4.0% intergenic (‑82/+40) yjdF ← / ← fumB DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
RA 4,345,642 T→C 4.0% intergenic (‑83/+39) yjdF ← / ← fumB DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
RA 4,345,644 A→C 3.8% intergenic (‑85/+37) yjdF ← / ← fumB DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
RA 4,394,323 T→G 13.3% V86G (GTC→GGC)  nnr → bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
RA 4,394,335 T→G 14.4% V90G (GTC→GGC)  nnr → bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
RA 4,396,209 T→A 3.3% D48E (GAT→GAA amiB → N‑acetylmuramoyl‑l‑alanine amidase II
RA 4,536,225 T→A 18.2% intergenic (+193/+390) yjhR → / ← nanS pseudogene, helicase family, putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase
RA 4,571,097 A→G 14.7% L160P (CTC→CCC)  yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 384783 385086 304 42 [40] [38] 41 yaiS/tauA putative PIG‑L family deacetylase/taurine ABC transporter periplasmic binding protein
* * ÷ NC_000913 583140 583824 685 43 [36] [39] 41 [appY] [appY]
* * ÷ NC_000913 1978500 1979280 781 42 [39] [39] 41 insB1–insA insB1, insA
* * ÷ NC_000913 3423862–3424231 3424453–3424237 7–592 42 [40] [39] 43 [rrlD] [rrlD]
* * ÷ NC_000913 3798868 3799539 672 42 [40] [40] 42 [waaU]–[waaZ] [waaU], [waaZ]
* * ÷ NC_000913 4233736 4235443 1708 42 [39] [40] 42 pgi pgi
* * ÷ NC_000913 4476728 4477170 443 41 [39] [38] 42 yjgL SopA‑central‑domain‑like hexapeptide repeat protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)116 (1.020) 77/432 NT 98.3% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 2 (0.020)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 = 36609990 (0.790)5 (0.050) 5/410 NT 5.3% coding (207/3075 nt) lacZ beta‑D‑galactosidase
?NC_000913 = 366105 93 (0.860)coding (201/3075 nt) lacZ beta‑D‑galactosidase
* ? NC_000913 458788 =4 (0.040)118 (1.060) 69/424 NT 96.8% intergenic (+88/‑100) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 608011 = NA (NA)intergenic (+23/‑54) hokE/insL1 toxic polypeptide, small/IS186 transposase
* ? NC_000913 = 4587992 (0.020)102 (0.910) 58/424 NT 98.1% intergenic (+99/‑89) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 = 609347 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? NC_000913 1207790 =92 (0.810)26 (0.250) 23/400 NT 26.2% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 61 (0.580)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 120780590 (0.790)26 (0.250) 21/400 NT 26.5% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 61 (0.580)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =1 (0.010)120 (1.050) 74/432 NT 99.2% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 12994980 (0.000)34 (0.300) 32/424 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1701107111 (0.970)3 (0.030) 3/414 NT 2.7% coding (151/1173 nt) malY PLP‑dependent beta‑cystathionase and maltose regulon regulator
?NC_000913 = 1701108 111 (1.020)coding (152/1173 nt) malY PLP‑dependent beta‑cystathionase and maltose regulon regulator
* ? NC_000913 = 19785020 (0.000)34 (0.310) 25/416 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 1979486 =0 (0.000)36 (0.320) 24/432 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)88 (0.770) 61/432 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 = 231924594 (0.830)3 (0.030) 3/416 NT 3.2% coding (630/2850 nt) rcsC hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
?NC_000913 = 2319262 91 (0.830)coding (613/2850 nt) rcsC hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
* ? NC_000913 = 2818273111 (0.970)3 (0.040) 3/274 NT 4.4% intergenic (‑1/+198) argY/argV tRNA‑Arg/tRNA‑Arg
?NC_000913 2818549 = 61 (0.850)intergenic (‑2/+2) argV/serV tRNA‑Arg/tRNA‑Ser
* ? NC_000913 = 4054136113 (0.990)4 (0.040) 4/414 NT 3.5% coding (1142/1410 nt) glnG fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
?NC_000913 = 4054156 112 (1.030)coding (1122/1410 nt) glnG fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
* ? NC_000913 = 416251490 (0.790)4 (0.040) 4/420 NT 4.1% coding (756/1101 nt) trmA tRNA m(5)U54 methyltransferase, SAM‑dependent, tmRNA m(5)U341 methyltransferase
?NC_000913 = 4162525 100 (0.900)coding (745/1101 nt) trmA tRNA m(5)U54 methyltransferase, SAM‑dependent, tmRNA m(5)U341 methyltransferase
* ? NC_000913 = 434563789 (0.780)4 (0.040) 4/420 NT 4.4% intergenic (‑78/+44) yjdF/fumB DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
?NC_000913 = 4345644 88 (0.800)intergenic (‑85/+37) yjdF/fumB DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
* ? NC_000913 4542683 =15 (0.130)6 (0.050) 6/414 NT 13.7% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542997 = 61 (0.560)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)