breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 15,318 G→A 5.0% intergenic (+20/‑127) dnaJ → / → insL1 chaperone Hsp40, DnaK co‑chaperone/IS186 transposase
RA 15,319 T→C 5.2% intergenic (+21/‑126) dnaJ → / → insL1 chaperone Hsp40, DnaK co‑chaperone/IS186 transposase
RA 83,587 A→C 5.6% intergenic (‑58/+35) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 83,590 G→T 5.6% intergenic (‑61/+32) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 87,881 A→G 5.4% intergenic (+33/‑147) ilvH → / → cra acetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism
RA 87,887 C→T 5.3% intergenic (+39/‑141) ilvH → / → cra acetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism
RA 120,154 Δ1 bp 5.1% intergenic (+19/+24) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
RA 120,158:1 +A 5.3% intergenic (+23/+20) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
RA 161,947 A→C 5.1% F29V (TTC→GTC)  ligT ← 2'‑5' RNA ligase
RA 164,581 A→C 12.4% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 192,713 T→C 6.5% intergenic (+133/‑159) pyrH → / → frr uridylate kinase/ribosome recycling factor
RA 252,636 T→C 5.3% A112A (GCT→GCC yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 349,717 T→C 10.9% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 373,332 C→G 5.5% P138A (CCA→GCA) ‡ mhpF → acetaldehyde‑CoA dehydrogenase II, NAD‑binding
RA 373,333 C→G 5.5% P138R (CCA→CGA) ‡ mhpF → acetaldehyde‑CoA dehydrogenase II, NAD‑binding
RA 375,247 A→G 5.7% intergenic (+366/‑212) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 464,354 C→A 5.0% intergenic (+46/‑48) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 464,356 A→T 5.6% intergenic (+48/‑46) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 556,019 T→A 5.2% intergenic (+23/+13) cysS → / ← ybcI cysteinyl‑tRNA synthetase/DUF457 family inner membrane protein
RA 604,752 G→T 5.6% intergenic (‑89/+19) ybdG ← / ← nfsB mechanosensitive channel protein, miniconductance/dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive
RA 604,753 A→C 5.6% intergenic (‑90/+18) ybdG ← / ← nfsB mechanosensitive channel protein, miniconductance/dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive
RA 632,070 T→G 7.6% intergenic (+71/‑112) cstA → / → ybdD carbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein
RA 632,075 G→A 7.1% intergenic (+76/‑107) cstA → / → ybdD carbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein
RA 632,076 G→C 7.6% intergenic (+77/‑106) cstA → / → ybdD carbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein
RA 632,077 T→C 7.7% intergenic (+78/‑105) cstA → / → ybdD carbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein
RA 632,082 C→A 7.8% intergenic (+83/‑100) cstA → / → ybdD carbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein
RA 710,170 C→T 9.7% intergenic (+54/+30) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 710,173 A→G 9.8% intergenic (+57/+27) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 756,968 C→G 5.7% H354Q (CAC→CAG sdhA → succinate dehydrogenase, flavoprotein subunit
RA 759,250 G→T 82.9% R182L (CGC→CTC)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 777,208 A→C 15.8% K289N (AAA→AAC tolA → membrane anchored protein in TolA‑TolQ‑TolR complex
RA 780,841:1 +T 7.6% intergenic (+1/‑2) valT → / → lysW tRNA‑Val/tRNA‑Lys
RA 781,254 A→T 9.4% intergenic (+32/‑115) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 781,269 C→T 7.1% intergenic (+47/‑100) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 781,668 C→T 8.7% intergenic (+16/‑417) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A
RA 781,670 A→T 8.2% intergenic (+18/‑415) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A
RA 781,672 A→G 8.4% intergenic (+20/‑413) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A
RA 850,993 T→C 5.1% intergenic (+28/+21) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 850,995 T→G 5.1% intergenic (+30/+19) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 850,996 C→A 5.1% intergenic (+31/+18) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 850,998 G→A 5.1% intergenic (+33/+16) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 1,015,912 T→A 8.7% intergenic (+30/+40) rmf → / ← fabA ribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase
RA 1,287,007 G→A 6.6% intergenic (+481/+59) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,287,161 G→A 6.4% noncoding (76/171 nt)
Q6* (CAA→TAA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein
RA 1,324,837 A→C 6.2% Y31S (TAT→TCT) ‡ yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,324,838 T→A 7.4% Y31* (TAT→TAA) ‡ yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,324,839 G→T 6.6% E32* (GAA→TAA)  yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,466,201 T→C 7.8% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,210 G→A 5.4% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,336 C→G 6.1% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,504 A→G 6.6% pseudogene (1113/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,562 G→A 9.7% pseudogene (1171/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,630 G→C 11.1% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,639 C→T 8.7% pseudogene (1248/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,498,892 A→T 5.0% intergenic (+17/‑46) ydcH → / → rimL DUF465 family protein/ribosomal‑protein‑L7/L12‑serine acetyltransferase
RA 1,498,896 A→T 5.3% intergenic (+21/‑42) ydcH → / → rimL DUF465 family protein/ribosomal‑protein‑L7/L12‑serine acetyltransferase
RA 1,544,868 G→A 5.9% pseudogene (847/951 nt) yddK ← pseudogene, leucine‑rich protein; putative glycoportein
RA 1,546,696 A→T 6.3% S158R (AGT→AGA yddG ← aromatic amino acid exporter
RA 1,597,272 T→A 6.2% pseudogene (716/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,597,281 A→G 6.0% pseudogene (707/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,629,435 G→A 5.1% intergenic (+17/+18) ydfZ → / ← ydfI selenoprotein, function unknown/putative NAD‑dependent D‑mannonate oxidoreductase
RA 1,708,709 T→A 7.7% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,844 C→G 10.7% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,847 G→A 6.4% E643E (GAG→GAA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,823,335 G→C 6.8% intergenic (+50/‑180) nadE → / → cho NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair
RA 1,823,338 G→C 6.5% intergenic (+53/‑177) nadE → / → cho NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair
RA 1,938,410 T→C 5.2% G254G (GGT→GGC pykA → pyruvate kinase II
RA 1,939,994 A→G 5.2% L67S (TTA→TCA)  lpxM ← myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 1,985,112 C→G 6.2% intergenic (‑43/+27) araG ← / ← araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
RA 2,044,725 T→A 5.4% intergenic (+101/‑213) asnT → / → yeeJ tRNA‑Asn/putative adhesin
RA 2,047,198 A→G 6.2% E754G (GAG→GGG)  yeeJ → putative adhesin
RA 2,068,407 A→G 5.6% pseudogene (13/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,068,408 T→A 7.3% pseudogene (12/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,068,409 G→C 7.1% pseudogene (11/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,068,410 T→A 6.6% pseudogene (10/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,068,411 C→T 5.5% pseudogene (9/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,139,709 G→A 5.6% intergenic (+224/+50) yegH → / ← asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,139,710 C→A 5.6% intergenic (+225/+49) yegH → / ← asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,154,847 A→G 5.7% S278G (AGT→GGT)  mdtA → multidrug efflux system, subunit A
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,177,245 G→T 11.4% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,219,632 G→A 8.4% intergenic (‑151/+60) osmF ← / ← bglX putative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,219,633 C→A 8.2% intergenic (‑152/+59) osmF ← / ← bglX putative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,219,639 G→T 10.3% intergenic (‑158/+53) osmF ← / ← bglX putative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,219,640 A→C 10.3% intergenic (‑159/+52) osmF ← / ← bglX putative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,219,646 T→G 6.0% intergenic (‑165/+46) osmF ← / ← bglX putative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,219,647 T→C 6.1% intergenic (‑166/+45) osmF ← / ← bglX putative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,229,177 A→C 15.4% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C
RA 2,270,602 T→C 5.1% intergenic (+57/‑124) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 2,304,649 C→T 5.7% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,013 A→G 9.1% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,375,193 A→G 5.1% L257P (CTG→CCG)  menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,428,423 A→G 7.4% I68I (ATT→ATC ubiX ← 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase
RA 2,496,268 C→G 5.8% S208R (AGC→AGG lpxP → palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
RA 2,644,954 T→C 5.9% intergenic (‑90/+59) ndk ← / ← pbpC multifunctional nucleoside diphosphate kinase and apyrimidinic endonuclease and 3'‑phosphodiesterase/penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
RA 2,750,073 G→T 6.4% intergenic (+13/+42) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein
RA 2,750,078 T→G 7.0% intergenic (+18/+37) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein
RA 2,750,081 C→A 6.6% intergenic (+21/+34) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein
RA 2,750,086 A→C 7.3% intergenic (+26/+29) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein
RA 2,761,620 T→G 6.6% K641Q (AAG→CAG)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
RA 2,761,623 C→A 6.6% V640L (GTA→TTA)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
RA 2,767,667 T→G 6.5% intergenic (+312/‑43) rnlB → / → yfjP CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,767,670 C→A 6.5% intergenic (+315/‑40) rnlB → / → yfjP CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,817,916 T→G 11.3% intergenic (‑56/+143) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,818,046 A→G 9.6% intergenic (‑186/+13) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,818,049 T→A 8.0% intergenic (‑189/+10) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,856,832 T→A 5.1% intergenic (‑26/‑261) ygbA ← / → mutS uncharacterized protein/methyl‑directed mismatch repair protein
RA 2,856,834 T→A 5.1% intergenic (‑28/‑259) ygbA ← / → mutS uncharacterized protein/methyl‑directed mismatch repair protein
RA 2,856,836 T→A 5.1% intergenic (‑30/‑257) ygbA ← / → mutS uncharacterized protein/methyl‑directed mismatch repair protein
RA 2,856,838 T→A 5.0% intergenic (‑32/‑255) ygbA ← / → mutS uncharacterized protein/methyl‑directed mismatch repair protein
RA 2,945,970 A→G 7.7% intergenic (‑128/+111) tcdA ← / ← mltA tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA/membrane‑bound lytic murein transglycosylase A
RA 2,945,971 A→C 8.1% intergenic (‑129/+110) tcdA ← / ← mltA tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA/membrane‑bound lytic murein transglycosylase A
RA 2,945,977 T→G 9.6% intergenic (‑135/+104) tcdA ← / ← mltA tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA/membrane‑bound lytic murein transglycosylase A
RA 2,945,978 C→A 9.6% intergenic (‑136/+103) tcdA ← / ← mltA tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA/membrane‑bound lytic murein transglycosylase A
RA 2,945,984 G→T 8.3% intergenic (‑142/+97) tcdA ← / ← mltA tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA/membrane‑bound lytic murein transglycosylase A
RA 2,945,985 C→T 9.0% intergenic (‑143/+96) tcdA ← / ← mltA tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA/membrane‑bound lytic murein transglycosylase A
RA 2,990,927 T→A 11.0% pseudogene (374/396 nt) ygeF → pseudogene
RA 3,057,349 C→G 5.1% L354L (CTG→CTC serA ← D‑3‑phosphoglycerate dehydrogenase
RA 3,269,956 T→C 7.5% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,270,143 C→T 9.5% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,368,189 A→G 5.4% A121A (GCA→GCG yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,458,110 C→A 100% T578T (ACC→ACA gspD → general secretory pathway component, cryptic
RA 3,482,325 G→A 100% R346H (CGC→CAC)  yheS → ABC‑F family protein predicted regulatory ATPase
RA 3,489,998 A→G 5.5% P61P (CCT→CCC argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
RA 3,504,880 A→C 8.5% intergenic (+97/+55) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,657,599 T→A 6.1% intergenic (+32/+387) hdeD → / ← arrS acid‑resistance membrane protein/antisense sRNA ArrS, function unknown
RA 3,657,601 G→C 5.6% intergenic (+34/+385) hdeD → / ← arrS acid‑resistance membrane protein/antisense sRNA ArrS, function unknown
RA 3,657,603 T→A 5.7% intergenic (+36/+383) hdeD → / ← arrS acid‑resistance membrane protein/antisense sRNA ArrS, function unknown
RA 3,658,002 C→T 7.9% noncoding (53/69 nt) arrS ← antisense sRNA ArrS, function unknown
RA 3,658,003 C→G 7.9% noncoding (52/69 nt) arrS ← antisense sRNA ArrS, function unknown
RA 3,658,004 A→G 7.9% noncoding (51/69 nt) arrS ← antisense sRNA ArrS, function unknown
JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,906,826 T→C 7.1% intergenic (‑259/+27) bglG ← / ← phoU transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator
RA 3,906,828 A→T 6.9% intergenic (‑261/+25) bglG ← / ← phoU transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator
RA 3,906,830 G→A 6.8% intergenic (‑263/+23) bglG ← / ← phoU transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator
RA 3,959,515 T→A 8.6% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 4,051,259 G→T 10.4% noncoding (224/245 nt) csrC → CsrC sRNA sequesters CsrA, a carbon flux regulator
RA 4,114,536 C→T 5.3% intergenic (‑64/+33) fpr ← / ← glpX ferredoxin‑NADP reductase; flavodoxin reductase/fructose 1,6‑bisphosphatase II
RA 4,114,537 G→T 5.7% intergenic (‑65/+32) fpr ← / ← glpX ferredoxin‑NADP reductase; flavodoxin reductase/fructose 1,6‑bisphosphatase II
RA 4,114,539 A→G 5.2% intergenic (‑67/+30) fpr ← / ← glpX ferredoxin‑NADP reductase; flavodoxin reductase/fructose 1,6‑bisphosphatase II
RA 4,153,435 T→C 5.0% intergenic (‑337/+261) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,153,447 A→G 7.4% intergenic (‑349/+249) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,162,150 T→G 5.5% intergenic (+20/+20) yijD → / ← trmA DUF1422 family inner membrane protein/tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase
RA 4,162,151 G→C 5.4% intergenic (+21/+19) yijD → / ← trmA DUF1422 family inner membrane protein/tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase
RA 4,162,152 G→C 5.5% intergenic (+22/+18) yijD → / ← trmA DUF1422 family inner membrane protein/tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase
RA 4,162,153 C→A 5.3% intergenic (+23/+17) yijD → / ← trmA DUF1422 family inner membrane protein/tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase
RA 4,296,060 C→T 33.6% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,325,948 A→G 8.8% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,366,854 T→G 9.2% intergenic (‑81/+37) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,366,855 C→A 9.0% intergenic (‑82/+36) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,460,476 A→G 6.1% intergenic (‑112/+46) nrdG ← / ← nrdD anaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase
RA 4,460,477 A→C 6.1% intergenic (‑113/+45) nrdG ← / ← nrdD anaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase
RA 4,460,483 T→A 6.2% intergenic (‑119/+39) nrdG ← / ← nrdD anaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase
RA 4,460,484 T→A 6.3% intergenic (‑120/+38) nrdG ← / ← nrdD anaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase
RA 4,473,927 C→A 100% A43S (GCC→TCC)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,520,500 Δ1 bp 5.6% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,520,504:1 +T 5.4% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,549,932 T→A 9.7% intergenic (+222/+21) fimH → / ← gntP minor component of type 1 fimbriae/fructuronate transporter
RA 4,614,321 A→G 5.6% intergenic (+61/+359) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,632,323 C→T 8.6% H531Y (CAT→TAT)  slt → lytic murein transglycosylase, soluble

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 448623 450707 2085 75 [71] [72] 80 [cyoC]–[cyoA] [cyoC],cyoB,[cyoA]
* * ÷ NC_000913 1166028 1167447 1420 78 [68] [73] 74 ndh ndh
* * ÷ NC_000913 3423750–3424234 3424564–3424238 5–815 74 [73] [71] 75 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =34 (0.220)107 (0.790) 56/246 NT 78.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 29 (0.210)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780526 (0.170)108 (0.800) 57/246 NT 80.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 29 (0.210)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)118 (0.790) 62/270 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 2270096117 (0.760)7 (0.050) 4/258 NT 5.6% coding (118/567 nt) mepS murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase
?NC_000913 = 2270134 126 (0.890)coding (156/567 nt) mepS murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase
* ? NC_000913 = 270056883 (0.540)6 (0.050) 3/240 NT 7.2% noncoding (49/77 nt) ohsC sRNA antisense regulator of shoB toxin
?NC_000913 = 2700581 84 (0.640)noncoding (62/77 nt) ohsC sRNA antisense regulator of shoB toxin
* ? NC_000913 = 2817942100 (0.650)13 (0.100) 10/238 NT 11.9% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?NC_000913 2818492 = 107 (0.820)noncoding (58/77 nt) argV tRNA‑Arg
* ? NC_000913 4542682 =121 (0.790)21 (0.150) 16/260 NT 14.1% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 143 (1.000)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 = 4542690117 (0.760)21 (0.150) 13/260 NT 14.3% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 143 (1.000)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)