breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,314,2150CT69.9% 137.1 / 88.4 116S352N (AGT→AAT) ndhENAD(P)H‑quinone oxidoreductase subunit 4L, chloroplastic
*USA300TCH1516_ALE512,6430AG49.5% 25.1 / inf 283intergenic (+711/‑6052)recR/speA_2Recombination protein RecR/Arginine decarboxylase
*USA300TCH1516_ALE111,5160CA41.0% 173.9 / 225.1 219Q196K (CAA→AAA) USA300HOU_RS00520putative lipoprotein
*USA300TCH1516_ALE111,5120AG39.7% 165.1 / 237.6 221S194S (TCA→TCGUSA300HOU_RS00520putative lipoprotein
*USA300TCH1516_ALE111,5600TC22.3% 256.4 / 63.4 132R210R (CGT→CGCUSA300HOU_RS00520putative lipoprotein
*USA300TCH1516_ALE2,679,2650AT20.0% 298.5 / 25.4 140intergenic (+137/‑252)USA300HOU_RS13790/ssaA2_4hypothetical protein/Staphylococcal secretory antigen ssaA2

Marginal new junction evidence (lowest skew 10 of 35 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 52489 =126 (1.040)33 (0.310) 7/250 6.7 25.0% intergenic (+99/‑574) USA300HOU_RS00215/USA300HOU_RS00220 hypothetical protein/hypothetical protein
?USA300TCH1516_ALE = 52983 86 (0.800)intergenic (+593/‑80) USA300HOU_RS00215/USA300HOU_RS00220 hypothetical protein/hypothetical protein
* ? USA300TCH1516_ALE 52495 =115 (0.950)146 (1.220)
+T
7/278 7.3 57.9% intergenic (+105/‑568) USA300HOU_RS00215/USA300HOU_RS00220 hypothetical protein/hypothetical protein
?USA300TCH1516_ALE = 52988 99 (0.820)intergenic (+598/‑75) USA300HOU_RS00215/USA300HOU_RS00220 hypothetical protein/hypothetical protein
* ? USA300TCH1516_ALE = 848580114 (0.940)6 (0.050)
+CTGAAAAAAAAAA
5/254 7.8 5.2% intergenic (+585/‑295) trxB/USA300HOU_RS04145 Thioredoxin reductase/Nucleotide‑binding protein
?USA300TCH1516_ALE 2866847 = 127 (1.050)intergenic (‑246/+207) USA300HOU_RS14695/noc_2 hypothetical protein/Nucleoid occlusion protein
* ? USA300TCH1516_ALE = 848580114 (0.940)5 (0.050)
+CTGAAAAAAAAAAA
3/252 8.9 3.6% intergenic (+585/‑295) trxB/USA300HOU_RS04145 Thioredoxin reductase/Nucleotide‑binding protein
?USA300TCH1516_ALE 2665075 = 182 (1.500)intergenic (+310/+128) pitA_2/USA300TCH1516_02535 L‑methionine sulfoximine/L‑methionine sulfone acetyltransferase/hypothetical protein
* ? USA300TCH1516_ALE 72990 =134 (1.110)7 (0.060) 3/254 9.0 5.6% intergenic (‑17/+69) arcD_1/arcA_1 Arginine/ornithine antiporter/Arginine deiminase
?USA300TCH1516_ALE 73054 = 115 (1.050)intergenic (‑81/+5) arcD_1/arcA_1 Arginine/ornithine antiporter/Arginine deiminase
* ? USA300TCH1516_ALE = 2618806162 (1.340)4 (0.040) 3/264 9.2 2.5% coding (291/1638 nt) pgcA Phosphoglucomutase
?USA300TCH1516_ALE = 2618808 153 (1.340)coding (293/1638 nt) pgcA Phosphoglucomutase
* ? USA300TCH1516_ALE = 84262695 (0.790)3 (0.030) 3/264 9.2 3.1% intergenic (+12/‑650) uvrA/hprK UvrABC system protein A/HPr kinase/phosphorylase
?USA300TCH1516_ALE = 842653 99 (0.870)intergenic (+39/‑623) uvrA/hprK UvrABC system protein A/HPr kinase/phosphorylase
* ? USA300TCH1516_ALE 2699163 =123 (1.020)45 (0.390)
+TAGTGT
3/268 9.3 26.4% intergenic (+67/‑224) copA/copZ Copper‑exporting P‑type ATPase A/Copper chaperone CopZ
?USA300TCH1516_ALE = 2699363 139 (1.150)intergenic (+267/‑24) copA/copZ Copper‑exporting P‑type ATPase A/Copper chaperone CopZ
* ? USA300TCH1516_ALE 2406948 =167 (1.380)8 (0.070) 3/266 9.3 5.0% coding (613/1716 nt) ureC Urease subunit alpha
?USA300TCH1516_ALE 2406995 = 144 (1.250)coding (660/1716 nt) ureC Urease subunit alpha
* ? USA300TCH1516_ALE = 1999809NA (NA)7 (0.060) 3/268 9.3 NA intergenic (‑1537/+1798) USA300TCH1516_01882/USA300TCH1516_01883 tRNA‑Val/tRNA‑Ala
?USA300TCH1516_ALE = 2177978 NA (NA)intergenic (+1740/+3555) ilvA/USA300TCH1516_02069 L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly