breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,736 A→T intergenic (+105/‑87) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,903 T→A D27E (GAT→GAA folA → type 3 dihydrofolate reductase
RA 50,280 T→C F153S (TTT→TCT)  folA → type 3 dihydrofolate reductase
RA 56,392 A→C Y240D (TAC→GAC)  lptD ← LPS assembly protein LptD
RA 108,362 A→G F242S (TTC→TCC)  zapD ← cell division protein ZapD
RA 115,488 T→C F90S (TTC→TCC)  ampD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD
RA 115,628 A→C T137P (ACG→CCG)  ampD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD
RA 130,698 A→T *866L (TAA→TTA)  acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA 139,618 A→G E118G (GAA→GGA)  yadG → ABC transporter ATP‑binding protein
RA 209,716 T→C I300T (ATC→ACC)  tilS → tRNA lysidine(34) synthetase TilS
RA 221,335 T→C noncoding (1078/1542 nt) BW25113_RS01010 → 16S ribosomal RNA
RA 231,042 A→C G76G (GGT→GGG gloB ← hydroxyacylglutathione hydrolase
RA 232,840 T→A V96E (GTG→GAG)  dnaQ → DNA polymerase III subunit epsilon
RA 264,689 (A)5→4 intergenic (‑18/+311) ykfA ← / ← perR GTPase family protein/LysR substrate‑binding domain‑containing protein
RA 297,509 G→C G30G (GGC→GGG paoB ← aldehyde oxidoreductase FAD‑binding subunit PaoB
RA 322,687 A→G I91T (ATC→ACC)  betA ← choline dehydrogenase
RA 340,444 (A)5→4 coding (558/816 nt) BW25113_RS01680 → hypothetical protein
RA 354,025 T→C E126G (GAA→GGA)  cynR ← transcriptional regulator CynR
RA 386,045 T→C pseudogene (339/1527 nt) BW25113_RS01915 → outer membrane autotransporter barrel domain‑containing protein
RA 444,519 G→A R527C (CGT→TGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 463,972 A→G D45G (GAC→GGC)  decR → DNA‑binding transcriptional regulator DecR
RA 465,464 T→C S380P (TCG→CCG)  mdlA → SmdA family multidrug ABC transporter permease/ATP‑binding protein
RA 476,525 A→G intergenic (‑361/+185) tomB ← / ← acrB Hha toxicity modulator TomB/efflux RND transporter permease AcrB
RA 502,414 T→C D42G (GAT→GGT)  ybaK ← Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase YbaK
RA 508,601 (C)6→7 coding (570/1293 nt) ybaT → APC family permease
RA 539,975 A→G D258D (GAT→GAC allC ← allantoate deiminase
RA 554,427 A→G intergenic (+217/‑3) fimA → / → fimC type 1 fimbrial major subunit FimA/type 1 fimbria chaperone FimC
RA 568,752 A→G G71G (GGA→GGG ybcO → DUF1364 domain‑containing protein
RA 579,943 (T)8→7 intergenic (+57/+193) appY → / ← ompT DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT
RA 592,895 A→C L103F (TTA→TTC cusF → Cu(+)/Ag(+) efflux RND transporter periplasmic metallochaperone CusF
RA 601,986 A→G F285S (TTC→TCC)  ybdK ← YbdK family carboxylate‑amine ligase
RA 609,731 A→G D40G (GAT→GGT)  entF → enterobactin non‑ribosomal peptide synthetase EntF
RA 661,892 A→C G594G (GGT→GGG mrdA ← peptidoglycan DD‑transpeptidase MrdA
RA 674,442 T→G T141P (ACT→CCT)  ybeT ← tetratricopeptide repeat protein
RA 802,549 T→G T63T (ACA→ACC ybhC ← putative acyl‑CoA thioester hydrolase
RA 874,237 C→T L14F (CTT→TTT)  yliI → aldose sugar dehydrogenase YliI
RA 886,189 A→G G7G (GGT→GGC grxA ← glutaredoxin 1
RA 950,036 (T)5→4 intergenic (‑114/+292) focA ← / ← ycaO formate transporter FocA/30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 951,809 T→G T94P (ACC→CCC)  ycaO ← 30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 977,631 A→C I377L (ATC→CTC)  ldtD → L,D‑transpeptidase
RA 979,260 T→C I52T (ATC→ACC)  gloC → hydroxyacylglutathione hydrolase GloC
RA 987,365 A→G N430D (AAT→GAT)  pepN → aminopeptidase N
RA 991,969 T→G L144F (TTA→TTC ssuA ← aliphatic sulfonate ABC transporter substrate‑binding protein SsuA
RA 1,021,663 (A)5→4 coding (1737/2055 nt) helD → DNA helicase IV
RA 1,054,234 A→G E232G (GAA→GGA)  torC → pentaheme c‑type cytochrome TorC
RA 1,060,988 (A)6→5 intergenic (+240/‑53) yccE → / → agp YccE family protein/bifunctional glucose‑1‑phosphatase/inositol phosphatase
RA 1,062,210 T→C G390G (GGT→GGC agp → bifunctional glucose‑1‑phosphatase/inositol phosphatase
RA 1,065,426 T→G T129P (ACC→CCC)  rutF ← pyrimidine utilization flavin reductase protein F
RA 1,076,727 (T)5→4 intergenic (+458/‑85) putP → / → BW25113_RS05295 sodium/proline symporter PutP/FTR1 family protein
RA 1,110,952 T→C intergenic (‑6/+166) mdtG ← / ← lpxL multidrug efflux MFS transporter MdtG/kdo(2)‑lipid IV(A) lauroyltransferase
RA 1,122,270 (A)6→5 coding (9/924 nt) yceM → Gfo/Idh/MocA family oxidoreductase
RA 1,153,703 A→T I127F (ATC→TTC)  ptsG → PTS glucose transporter subunit IIBC
RA 1,167,328 T→C E698G (GAA→GGA)  mfd ← transcription‑repair coupling factor
RA 1,223,232 (A)8→7 coding (63/294 nt) BW25113_RS06140 → YcgL domain‑containing protein
RA 1,240,670 Δ1 bp coding (55/441 nt) BW25113_RS06225 → hypothetical protein
RA 1,259,173 A→G T2A (ACC→GCC)  hemA → glutamyl‑tRNA reductase
RA 1,276,251 Δ1 bp coding (932/3744 nt) narG → nitrate reductase subunit alpha
RA 1,300,524 Δ1 bp coding (504/1005 nt) oppF → murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF
RA 1,320,565 G→A Q521Q (CAG→CAA yciQ → DUF2207 domain‑containing protein
RA 1,325,886 T→C G194G (GGT→GGC topA → type I DNA topoisomerase
RA 1,331,016 A→G Y310C (TAT→TGT)  acnA → aconitate hydratase AcnA
RA 1,336,062 A→G intergenic (+78/‑116) lapB → / → pyrF lipopolysaccharide assembly protein LapB/orotidine‑5'‑phosphate decarboxylase
RA 1,363,696 T→C F16S (TTC→TCC)  pspD → phage shock protein PspD
RA 1,364,301 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
RA 1,392,826 T→C Q18Q (CAA→CAG uspE ← universal stress protein UspE
RA 1,394,663 T→C G456G (GGA→GGG abgT ← p‑aminobenzoyl‑glutamate transporter
RA 1,402,000 A→G I18T (ATC→ACC)  dgcM ← diguanylate cyclase DgcM
RA 1,417,788 (C)6→7 coding (69/120 nt) BW25113_RS26130 ← hypothetical protein
RA 1,423,602 T→C R99R (CGT→CGC stfR → prophage tail fiber N‑terminal domain‑containing protein
RA 1,431,536 T→C E1169G (GAA→GGA)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,434,377 A→G F222S (TTC→TCC)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,454,774 A→G D16G (GAT→GGT)  paaI → hydroxyphenylacetyl‑CoA thioesterase PaaI
RA 1,494,123 T→C E195G (GAA→GGA)  ydcK ← YdcK family protein
RA 1,499,566 A→G S258P (TCC→CCC)  ydcO ← BenE family transporter YdcO
RA 1,502,076 A→G N347D (AAC→GAC)  rlhA → 23S rRNA 5‑hydroxycytidine C2501 synthase
RA 1,503,965 T→C D67D (GAT→GAC hicB → type II toxin‑antitoxin system antitoxin HicB
RA 1,505,430 A→G K391E (AAA→GAA)  ydcR → PLP‑dependent aminotransferase family protein
RA 1,507,468 C→A P132Q (CCG→CAG)  ydcT → ABC transporter ATP‑binding protein
RA 1,524,815 T→C intergenic (‑54/‑28) BW25113_RS25600 ← / → BW25113_RS07655 protein YncO/ISAs1 family transposase
RA 1,531,275 G→A L98L (CTG→TTG)  narW ← nitrate reductase molybdenum cofactor assembly chaperone
RA 1,547,935 A→G V54A (GTA→GCA)  adhP ← alcohol dehydrogenase AdhP
RA 1,558,439 A→G Y518H (TAT→CAT)  dosP ← oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
RA 1,568,198 A→G F417S (TTC→TCC)  pqqL ← insulinase family protein
RA 1,573,157 A→T I148N (ATC→AAC)  yddA ← ABC transporter ATP‑binding protein/permease
RA 1,575,825 A→T D319E (GAT→GAA ydeN ← sulfatase
RA 1,576,388 A→G S132P (TCC→CCC)  ydeN ← sulfatase
RA 1,579,109 A→C G545G (GGT→GGG ydeP ← acid resistance putative oxidoreductase YdeP
RA 1,613,753 (A)6→5 coding (377/435 nt) marR → multiple antibiotic resistance transcriptional regulator MarR
RA 1,632,437 (T)7→6 intergenic (‑71/+8) BW25113_RS08135 ← / ← BW25113_RS25620 hypothetical protein/hypothetical protein
RA 1,634,151 Δ1 bp coding (164/534 nt) rrrQ ← lysozyme
RA 1,649,476 A→G intergenic (‑78/+128) rspA ← / ← ynfA starvation‑sensing protein RspA/YnfA family protein
RA 1,659,576 T→C F14S (TTC→TCC)  clcB → voltage‑gated ClC‑type chloride channel ClcB
RA 1,662,649 T→G H58P (CAC→CCC)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,671,899 T→G T88P (ACG→CCG)  pntA ← Re/Si‑specific NAD(P)(+) transhydrogenase subunit alpha
RA 1,673,542 A→G K287E (AAG→GAG)  ydgH → DUF1471 family protein YdgH
RA 1,717,498 G→C G374A (GGC→GCC)  ydhK → FUSC family protein
RA 1,761,086 A→C R619R (CGT→CGG ydiJ ← FAD‑binding and (Fe‑S)‑binding domain‑containing protein
RA 1,763,148 A→G intergenic (‑206/‑183) ydiJ ← / → ydiK FAD‑binding and (Fe‑S)‑binding domain‑containing protein/AI‑2E family transporter YdiK
RA 1,803,201 A→G V97A (GTC→GCC)  yniB ← YniB family protein
RA 1,807,028 T→C E121G (GAA→GGA)  BW25113_RS09050 ← hypothetical protein
RA 1,812,807 (C)7→8 intergenic (‑50/+55) celF ← / ← chbR 6‑phospho‑beta‑glucosidase/transcriptional regulator ChbR
RA 1,838,813 T→C I106V (ATT→GTT)  ynjI ← DUF1266 domain‑containing protein
RA 1,840,928 T→C E97G (GAA→GGA)  topB ← DNA topoisomerase III
RA 1,848,011 T→C T76A (ACT→GCT)  ydjE ← MFS transporter
RA 1,854,126 T→C M121V (ATG→GTG)  ydjK ← MFS transporter
RA 1,877,816 T→C pseudogene (408/1482 nt) yeaV → BCCT family transporter YeaV
RA 1,897,230 A→G D309G (GAT→GGT)  manX → PTS mannose transporter subunit IIAB
RA 1,900,885 A→G S145P (TCC→CCC)  rlmA ← 23S rRNA (guanine(745)‑N(1))‑methyltransferase
RA 1,903,061 A→T intergenic (‑38/‑121) mgrB ← / → yobH PhoP/PhoQ regulator MgrB/YobH family protein
RA 1,927,010 T→C K2E (AAA→GAA)  kdgA ← bifunctional 4‑hydroxy‑2‑oxoglutarate aldolase/2‑dehydro‑3‑deoxy‑phosphogluconate aldolase
RA 1,934,547 T→C intergenic (‑97/+23) lpxM ← / ← mepM lauroyl‑Kdo(2)‑lipid IV(A) myristoyltransferase/murein DD‑endopeptidase MepM
RA 1,941,604 A→C G10G (GGT→GGG ruvC ← crossover junction endodeoxyribonuclease RuvC
RA 1,961,177 C→A A40S (GCC→TCC)  cheZ ← protein phosphatase CheZ
RA 1,965,782 T→G E389D (GAA→GAC tar ← methyl‑accepting chemotaxis protein II
RA 1,972,691 A→G intergenic (‑237/‑543) flhD ← / → uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
RA 1,980,420 A→G G5G (GGA→GGG ftnB → non‑heme ferritin‑like protein
RA 2,003,480 T→C F60S (TTC→TCC)  yedK → SOS response‑associated peptidase
RA 2,038,183 (T)5→4 intergenic (+78/‑236) BW25113_RS10330 → / → yeeJ tRNA‑Asn/inverse autotransporter adhesin YeeJ
RA 2,057,664 T→C G95G (GGA→GGG cobT ← nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
RA 2,061,342 T→A intergenic (‑576/‑774) BW25113_RS10415 ← / → BW25113_RS24005 IS5‑like element IS5 family transposase/TonB‑dependent receptor plug domain‑containing protein
RA 2,064,259 T→G V93G (GTG→GGG)  BW25113_RS10440 → GTPase family protein
RA 2,102,646 T→C G140G (GGA→GGG wzx ← O16 family O‑antigen flippase
RA 2,131,004 A→G intergenic (‑280/‑379) wza ← / → yegH polysaccharide export protein Wza/TerC family protein
RA 2,174,381 T→G C270G (TGC→GGC)  yegU → ADP‑ribosylglycohydrolase family protein
RA 2,176,805 T→G D109A (GAC→GCC)  yegX ← glycoside hydrolase family 25 protein
RA 2,189,312 A→G T512A (ACC→GCC)  metG → methionine‑‑tRNA ligase
RA 2,197,981 T→C intergenic (+213/‑94) BW25113_RS11050 → / → yehL hypothetical protein/MoxR family ATPase
RA 2,202,171 G→C W242C (TGG→TGC yehP → VWA domain‑containing protein
RA 2,220,044 A→G S236P (TCG→CCG)  yohF ← SDR family oxidoreductase
RA 2,229,956 A→G K405E (AAA→GAA)  preA → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
RA 2,283,874 Δ1 bp intergenic (‑350/‑105) BW25113_RS11435 ← / → narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
RA 2,284,519 T→C S181P (TCC→CCC)  narP → nitrate/nitrite response regulator protein NarP
RA 2,345,414 T→G H147P (CAC→CCC)  glpT ← glycerol‑3‑phosphate transporter
RA 2,358,446 Δ1 bp coding (414/426 nt) nudI → nucleoside triphosphatase NudI
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 2,410,868 A→T G103G (GGA→GGT yfcC → putative basic amino acid antiporter YfcC
RA 2,425,778 T→C E215G (GAA→GGA)  folC ← bifunctional tetrahydrofolate synthase/dihydrofolate synthase
RA 2,434,529 A→C F186V (TTT→GTT)  fabB ← beta‑ketoacyl‑ACP synthase I
RA 2,505,777 T→C I137V (ATC→GTC)  mntH ← Nramp family divalent metal transporter
RA 2,526,050 A→G T95A (ACC→GCC)  cysK → cysteine synthase A
RA 2,536,106 T→C E261G (GAA→GGA)  cysP ← thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
RA 2,554,121 A→C N169T (AAC→ACC)  BW25113_RS12740 → hypothetical protein
RA 2,591,366 C→T D287N (GAC→AAC)  bamC ← outer membrane protein assembly factor BamC
RA 2,661,508 T→G T250P (ACC→CCC)  hcaR ← DNA‑binding transcriptional regulator HcaR
RA 2,670,359 T→C G454G (GGA→GGG yphE ← sugar ABC transporter ATP‑binding protein
RA 2,678,723 A→C G48G (GGT→GGG glyA ← serine hydroxymethyltransferase
RA 2,739,096 T→C E61G (GAA→GGA)  rimM ← ribosome maturation factor RimM
RA 2,751,237 A→G V241V (GTT→GTC BW25113_RS13685 ← hypothetical protein
RA 2,753,169 T→C S276P (TCA→CCA)  yfjI → YfjI family protein
RA 2,759,232 (G)6→7 intergenic (‑97/‑45) yfjM ← / → rnlA DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA
RA 2,778,934 (T)6→5 intergenic (‑564/+187) BW25113_RS25705 ← / ← BW25113_RS13825 DUF5507 domain‑containing protein/tRNA‑Ile
RA 2,797,377 (T)5→4 coding (517/960 nt) nrdF → class 1b ribonucleoside‑diphosphate reductase subunit beta
RA 2,801,112 A→C T208P (ACC→CCC)  proX → glycine betaine/L‑proline ABC transporter substrate‑binding protein ProX
RA 2,814,267 A→G A368A (GCT→GCC alaS ← alanine‑‑tRNA ligase
RA 2,818,451 T→C E162G (GAA→GGA)  mltB ← lytic murein transglycosylase B
RA 2,819,095 T→C intergenic (‑160/‑96) mltB ← / → srlA lytic murein transglycosylase B/PTS glucitol/sorbitol transporter subunit IIC
RA 2,833,262 T→G V127G (GTC→GGC)  ascF → PTS cellobiose/arbutin/salicin transporter subunit IIBC
RA 2,849,315 A→C Y540S (TAC→TCC)  flhA → formate hydrogenlyase transcriptional activator FlhA
RA 2,849,816 T→C E117G (GAA→GGA)  ygbA ← nitrous oxide‑stimulated promoter family protein
RA 2,859,998 T→C I305V (ATT→GTT)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,882,413 A→C V349G (GTT→GGT)  cysI ← assimilatory sulfite reductase (NADPH) hemoprotein subunit
RA 2,888,711 A→G V143A (GTC→GCC)  ygcQ ← electron transfer flavoprotein subunit alpha/FixB family protein
RA 2,982,555 T→C F232S (TTC→TCC)  yqeI → transcriptional regulator
RA 2,995,567 A→G T635A (ACC→GCC)  xdhA → xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,011,010 A→G E531G (GAA→GGA)  ygfK → putative selenate reductase subunit YgfK
RA 3,098,336 Δ1 bp coding (545/1080 nt) mltC → membrane‑bound lytic murein transglycosylase MltC
RA 3,103,871 (A)7→6 intergenic (+71/+78) BW25113_RS15410 → / ← yghD tRNA‑Phe/GspM family type II secretion system protein YghD
RA 3,113,649 T→G K330N (AAA→AAC glcA ← glycolate permease GlcA
RA 3,179,271 G→A A167T (GCC→ACC)  ygiL → fimbrial‑like protein
RA 3,273,721 A→G E49G (GAA→GGA)  agaV → PTS N‑acetylgalactosamine transporter subunit IIB
RA 3,369,157 T→C G310G (GGT→GGC dcuC → anaerobic C4‑dicarboxylate transporter DcuC
RA 3,458,386 A→G E369G (GAA→GGA)  gspL → type II secretion system protein GspL
RA 3,475,701 T→C S352P (TCG→CCG)  yheS → ABC transporter ATP‑binding protein
RA 3,535,339 T→C F525S (TTC→TCC)  feoB → Fe(2+) transporter permease subunit FeoB
RA 3,576,634 A→G R79R (CGA→CGG BW25113_RS17825 → YrhA family protein
RA 3,579,168 T→C E339G (GAA→GGA)  ggt ← gamma‑glutamyltransferase
RA 3,717,969 T→C E237G (GAA→GGA)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,728,024 A→C S315S (TCA→TCC xylH → xylose ABC transporter permease XylH
RA 3,771,044 A→G K96E (AAA→GAA)  lldP → L‑lactate permease
RA 3,785,687 G→C G75G (GGC→GGG kbl ← glycine C‑acetyltransferase
RA 3,786,467 T→C I193V (ATT→GTT)  yibB ← protein YibB
RA 3,786,991 T→C D18G (GAT→GGT)  yibB ← protein YibB
RA 3,791,182 A→C Y292S (TAC→TCC)  waaL → O‑antigen ligase
RA 3,798,416 (T)8→7 coding (61/936 nt) rfaS ← LPS core biosynthesis protein RfaS
RA 3,840,691 T→G N432H (AAC→CAC)  uhpC ← MFS transporter family glucose‑6‑phosphate receptor UhpC
RA 3,848,051 T→G F257C (TTT→TGT)  emrD → multidrug efflux MFS transporter EmrD
RA 3,852,078 T→G T467P (ACG→CCG)  yidK ← solute:sodium symporter family transporter
RA 3,896,621 A→G D147D (GAT→GAC bglB ← 6‑phospho‑beta‑glucosidase BglB
RA 3,898,828 (T)5→4 coding (130/1878 nt) bglF ← PTS beta‑glucoside transporter subunit IIABC
RA 3,901,121 A→G D202D (GAT→GAC pstB ← phosphate ABC transporter ATP‑binding protein PstB
RA 3,931,448 G→T L265F (TTG→TTT rbsK → ribokinase
RA 4,034,572 T→G Q74H (CAA→CAC mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein MobB
RA 4,057,873 C→G W418C (TGG→TGC yihO ← MFS transporter
RA 4,064,564 A→G Y185H (TAT→CAT)  yihS ← sulfoquinovose isomerase
RA 4,073,163 A→T D26V (GAT→GTT)  BW25113_RS20185 → hypothetical protein
RA 4,084,653 A→G G358G (GGT→GGC frvB ← PTS fructose‑like transporter subunit IIBC
RA 4,087,693 T→C F10F (TTT→TTC rhaS → HTH‑type transcriptional activator RhaS
RA 4,108,830 A→G H143H (CAT→CAC rraA ← ribonuclease E activity regulator RraA
RA 4,117,071 T→C F44S (TTC→TCC)  rpmE → 50S ribosomal protein L31
RA 4,153,816 T→C S84P (TCA→CCA)  btuB → TonB‑dependent vitamin B12 receptor BtuB
RA 4,180,586 (A)7→6 intergenic (+333/+77) yjaZ → / ← thiH heat‑shock protein C/2‑iminoacetate synthase ThiH
RA 4,211,170 (T)8→7 coding (1246/2187 nt) espL4 ← type III secretion system effector EspL4
RA 4,215,724 T→G W657G (TGG→GGG)  metH → methionine synthase
RA 4,306,692 A→T I115N (ATC→AAC)  phnM ← alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase
RA 4,310,789 C→T A92A (GCG→GCA phnH ← phosphonate C‑P lyase system protein PhnH
RA 4,350,381 G→C G457G (GGC→GGG cadC ← lysine decarboxylation/transport transcriptional activator CadC
RA 4,367,563 T→C intergenic (‑24/+65) blc ← / ← ampC lipocalin Blc/BlaEC family class C beta‑lactamase
RA 4,494,539 T→C F222S (TTC→TCC)  nanX → MFS transporter
RA 4,527,151 T→C Q87Q (CAA→CAG nanS ← 9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
RA 4,543,103 A→G E129G (GAA→GGA)  uxuB → fructuronate reductase
RA 4,593,447 T→C S52P (TCC→CCC)  yjjQ → response regulator transcription factor
RA 4,613,222 C→G R238P (CGC→CCC)  lplA ← lipoate‑‑protein ligase LplA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 69539 69549 11 3 [0] [0] 3 thiP thiamine/thiamine pyrophosphate ABC transporter permease ThiP
* * ÷ NZ_CP009273 182806 182822 17 2 [1] [0] 2 glnD bifunctional uridylyltransferase/uridylyl‑removing protein GlnD
* * ÷ NZ_CP009273 227188 227213 26 2 [1] [1] 2 yafC DNA‑binding transcriptional regulator YafC
* * ÷ NZ_CP009273 356981 357574 594 2 [0] [0] 2 [lacA] [lacA]
* * ÷ NZ_CP009273 385341 385394 54 2 [1] [1] 2 hemB/BW25113_RS01915 porphobilinogen synthase/outer membrane autotransporter barrel domain‑containing protein
* * ÷ NZ_CP009273 437702 437733 32 3 [1] [1] 2 thiI tRNA 4‑thiouridine(8) synthase ThiI
* * ÷ NZ_CP009273 538013 538044 32 2 [1] [1] 2 glxK glycerate 3‑kinase
* * ÷ NZ_CP009273 585059 585061 3 2 [0] [0] 2 nfrA bacteriophage adsorption protein NfrA
* * ÷ NZ_CP009273 613823 613836 14 3 [1] [1] 2 wzz(fepE) LPS O‑antigen length regulator Wzz(fepE)
* * ÷ NZ_CP009273 679631 679709 79 2 [1] [0] 2 rihA pyrimidine‑specific ribonucleoside hydrolase RihA
* * ÷ NZ_CP009273 681289 681301 13 2 [1] [1] 3 gltK glutamate/aspartate ABC transporter permease GltK
* * ÷ NZ_CP009273 690114 690154 41 2 [1] [1] 2 miaB tRNA (N6‑isopentenyl adenosine(37)‑C2)‑methylthiotransferase MiaB
* * ÷ NZ_CP009273 690245 690278 34 2 [1] [1] 3 miaB tRNA (N6‑isopentenyl adenosine(37)‑C2)‑methylthiotransferase MiaB
* * ÷ NZ_CP009273 703451 703524 74 3 [1] [1] 2 glnS/chiP glutamine‑‑tRNA ligase/chitoporin
* * ÷ NZ_CP009273 710289 710300 12 2 [1] [1] 2 pgm phosphoglucomutase (alpha‑D‑glucose‑1,6‑bisphosphate‑dependent)
* * ÷ NZ_CP009273 716359 716520 162 2 [1] [1] 2 [kdpE] [kdpE]
* * ÷ NZ_CP009273 717639 717741 103 2 [1] [1] 3 kdpD two‑component system sensor histidine kinase KdpD
* * ÷ NZ_CP009273 718841 718861 21 2 [1] [0] 2 kdpD two‑component system sensor histidine kinase KdpD
* * ÷ NZ_CP009273 719523 719610 88 2 [1] [1] 2 kdpD two‑component system sensor histidine kinase KdpD
* * ÷ NZ_CP009273 731622 731638 17 2 [1] [0] 2 BW25113_RS03640 hypothetical protein
* * ÷ NZ_CP009273 774668 774710 43 5 [1] [1] 2 pal peptidoglycan‑associated lipoprotein Pal
* * ÷ NZ_CP009273 797369 797399 31 2 [1] [1] 3 ybhI anion permease
* * ÷ NZ_CP009273 800072 800132 61 2 [1] [1] 2 ybhJ hydratase
* * ÷ NZ_CP009273 806257 806274 18 2 [1] [1] 2 bioF 8‑amino‑7‑oxononanoate synthase
* * ÷ NZ_CP009273 842088 842104 17 2 [1] [1] 2 glnP glutamine ABC transporter permease GlnP
* * ÷ NZ_CP009273 852215 852229 15 2 [1] [1] 2 ybiT ABC‑F family ATPase
* * ÷ NZ_CP009273 898274 898283 10 2 [0] [1] 4 artJ arginine ABC transporter substrate‑binding protein
* * ÷ NZ_CP009273 905913 905960 48 2 [1] [1] 2 poxB ubiquinone‑dependent pyruvate dehydrogenase
* * ÷ NZ_CP009273 933814 933829 16 2 [1] [1] 2 rarA replication‑associated recombination protein RarA
* * ÷ NZ_CP009273 943074 943086 13 4 [0] [1] 2 ycaM amino acid permease
* * ÷ NZ_CP009273 972376 972405 30 3 [1] [0] 2 mukB chromosome partition protein MukB
* * ÷ NZ_CP009273 984606 984633 28 2 [1] [1] 2 [pncB] [pncB]
* * ÷ NZ_CP009273 985368 985389 22 3 [0] [1] 2 pncB nicotinate phosphoribosyltransferase
* * ÷ NZ_CP009273 988185 988210 26 2 [1] [1] 2 pepN aminopeptidase N
* * ÷ NZ_CP009273 990762 990836 75 2 [1] [1] 2 ssuD FMNH2‑dependent alkanesulfonate monooxygenase
* * ÷ NZ_CP009273 990927 990970 44 2 [1] [1] 3 ssuD FMNH2‑dependent alkanesulfonate monooxygenase
* * ÷ NZ_CP009273 999399 999444 46 2 [1] [0] 2 ycbF fimbrial chaperone
* * ÷ NZ_CP009273 1000067 1000157 91 2 [1] [1] 2 [ycbF] [ycbF]
* * ÷ NZ_CP009273 1030835 1030848 14 2 [1] [1] 2 hyaC Ni/Fe‑hydrogenase b‑type cytochrome subunit
* * ÷ NZ_CP009273 1041923 1041938 16 2 [1] [1] 3 gfcD YjbH domain‑containing protein
* * ÷ NZ_CP009273 1075759 1075781 23 2 [1] [1] 2 putP sodium/proline symporter PutP
* * ÷ NZ_CP009273 1079216 1079244 29 4 [1] [0] 2 efeB iron uptake transporter deferrochelatase/peroxidase subunit
* * ÷ NZ_CP009273 1117521 1117582 62 3 [1] [1] 2 pyrC dihydroorotase
* * ÷ NZ_CP009273 1120093 1120124 32 2 [1] [1] 2 mdtH multidrug efflux MFS transporter MdtH
* * ÷ NZ_CP009273 1129710 1129745 36 2 [1] [1] 2 flgF flagellar basal‑body rod protein FlgF
* * ÷ NZ_CP009273 1137823 1137874 52 2 [1] [1] 2 rne ribonuclease E
* * ÷ NZ_CP009273 1138325 1138357 33 2 [1] [0] 2 rne ribonuclease E
* * ÷ NZ_CP009273 1165276 1165287 12 2 [1] [0] 2 ldtC L,D‑transpeptidase LdtC
* * ÷ NZ_CP009273 1165689 1165787 99 2 [1] [1] 2 ldtC L,D‑transpeptidase LdtC
* * ÷ NZ_CP009273 1172280 1172340 61 2 [1] [1] 2 lolD lipoprotein‑releasing ABC transporter ATP‑binding protein LolD
* * ÷ NZ_CP009273 1172441 1172534 94 2 [1] [1] 2 lolD lipoprotein‑releasing ABC transporter ATP‑binding protein LolD
* * ÷ NZ_CP009273 1231894 1231948 55 2 [1] [1] 2 ycgB SpoVR family protein
* * ÷ NZ_CP009273 1234857 1234887 31 3 [1] [1] 4 dadX catabolic alanine racemase DadX
* * ÷ NZ_CP009273 1240301 1240338 38 2 [1] [0] 3 ymgE GlsB/YeaQ/YmgE family stress response membrane protein
* * ÷ NZ_CP009273 1258527 1258590 64 2 [1] [1] 2 lolB lipoprotein insertase outer membrane protein LolB
* * ÷ NZ_CP009273 1260097 1260131 35 2 [0] [1] 2 hemA glutamyl‑tRNA reductase
* * ÷ NZ_CP009273 1265239 1265272 34 2 [1] [0] 2 [BW25113_RS06335] [BW25113_RS06335]
* * ÷ NZ_CP009273 1269186 1269204 19 2 [1] [1] 3 ychN/ychO DsrE/F sulfur relay family protein YchN/inverse autotransporter invasin YchO
* * ÷ NZ_CP009273 1275277 1275289 13 2 [1] [1] 2 narK/narG nitrate transporter NarK/nitrate reductase subunit alpha
* * ÷ NZ_CP009273 1279167 1279187 21 2 [0] [1] 2 narH nitrate reductase subunit beta
* * ÷ NZ_CP009273 1294080 1294145 66 2 [1] [1] 3 ychE NAAT family transporter YchE
* * ÷ NZ_CP009273 1301887 1302023 137 2 [1] [1] 3 cls cardiolipin synthase
* * ÷ NZ_CP009273 1305634 1305663 30 2 [1] [1] 2 tonB TonB system transport protein TonB
* * ÷ NZ_CP009273 1311179 1311246 68 2 [1] [1] 2 trpA tryptophan synthase subunit alpha
* * ÷ NZ_CP009273 1314717 1314786 70 2 [1] [1] 2 trpD bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
* * ÷ NZ_CP009273 1314976 1315013 38 2 [1] [1] 2 trpD bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
* * ÷ NZ_CP009273 1316485 1316504 20 2 [1] [1] 2 trpE anthranilate synthase component I
* * ÷ NZ_CP009273 1316595 1316634 40 2 [1] [1] 2 trpE anthranilate synthase component I
* * ÷ NZ_CP009273 1320837 1320952 116 3 [1] [1] 2 [yciQ] [yciQ]
* * ÷ NZ_CP009273 1326417 1326453 37 2 [1] [1] 2 topA type I DNA topoisomerase
* * ÷ NZ_CP009273 1327347 1327388 42 2 [0] [1] 2 topA type I DNA topoisomerase
* * ÷ NZ_CP009273 1327649 1327684 36 2 [1] [1] 2 topA type I DNA topoisomerase
* * ÷ NZ_CP009273 1329533 1329583 51 2 [1] [1] 2 [ymiA]–[BW25113_RS06660] [ymiA],[BW25113_RS06660]
* * ÷ NZ_CP009273 1330459 1330568 110 2 [1] [1] 2 acnA aconitate hydratase AcnA
* * ÷ NZ_CP009273 1330896 1330916 21 2 [0] [1] 2 acnA aconitate hydratase AcnA
* * ÷ NZ_CP009273 1331592 1331702 111 2 [1] [1] 2 acnA aconitate hydratase AcnA
* * ÷ NZ_CP009273 1342211 1342229 19 2 [0] [0] 3 rnb exoribonuclease II
* * ÷ NZ_CP009273 1360668 1360708 41 2 [1] [1] 2 puuE 4‑aminobutyrate transaminase
* * ÷ NZ_CP009273 1361841 1361923 83 2 [1] [1] 2 pspF phage shock protein operon transcriptional activator
* * ÷ NZ_CP009273 1363487 1363508 22 2 [1] [1] 2 pspC envelope stress response membrane protein PspC
* * ÷ NZ_CP009273 1403992 1403999 8 2 [1] [1] 3 dbpA ATP‑dependent RNA helicase DbpA
* * ÷ NZ_CP009273 1417041 1417047 7 2 [0] [1] 2 BW25113_RS07140 DUF1627 domain‑containing protein
* * ÷ NZ_CP009273 1424231 1424262 32 2 [1] [1] 2 stfR prophage tail fiber N‑terminal domain‑containing protein
* * ÷ NZ_CP009273 1447995 1448007 13 2 [1] [1] 2 paaZ/paaA phenylacetic acid degradation bifunctional protein PaaZ/1,2‑phenylacetyl‑CoA epoxidase subunit A
* * ÷ NZ_CP009273 1480451 1480465 15 2 [1] [1] 2 hrpA ATP‑dependent RNA helicase HrpA
* * ÷ NZ_CP009273 1528928 1528945 18 2 [1] [1] 2 nhoA N‑hydroxyarylamine O‑acetyltransferase
* * ÷ NZ_CP009273 1587809 1587811 3 2 [0] [1] 5 BW25113_RS07915 ECSE_1600 family autotransporter
* * ÷ NZ_CP009273 1594810 1594827 18 2 [1] [1] 3 lsrR transcriptional regulator LsrR
* * ÷ NZ_CP009273 1620625 1620653 29 2 [1] [1] 2 dcp peptidyl‑dipeptidase Dcp
* * ÷ NZ_CP009273 1694411 1694430 20 3 [1] [1] 2 malX PTS maltose transporter subunit IICB
* * ÷ NZ_CP009273 1893636 1893672 37 3 [1] [1] 2 pdeD cyclic diguanylate phosphodiesterase
* * ÷ NZ_CP009273 1973051 1973109 59 3 [1] [1] 2 flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* * ÷ NZ_CP009273 2010179 2010242 64 2 [1] [1] 2 fliI flagellar protein export ATPase FliI
* * ÷ NZ_CP009273 2149192 2149246 55 3 [1] [0] 2 mdtB multidrug efflux RND transporter permease subunit MdtB
* * ÷ NZ_CP009273 2157559 2157584 26 3 [0] [1] 3 baeS two‑component system sensor histidine kinase BaeS
* * ÷ NZ_CP009273 2158160 2158248 89 2 [1] [1] 2 baeR two‑component system response regulator BaeR
* * ÷ NZ_CP009273 2161847 2161860 14 2 [0] [1] 3 BW25113_RS10880/yegS hypothetical protein/lipid kinase YegS
* * ÷ NZ_CP009273 2192859 2192906 48 2 [1] [1] 2 BW25113_RS25680 WGR and DUF4132 domain‑containing protein
* * ÷ NZ_CP009273 2256379 2256454 76 2 [1] [1] 2 fruB fused PTS fructose transporter subunit IIA/HPr protein
* * ÷ NZ_CP009273 2300671 2300695 25 2 [1] [1] 2 yojI microcin J25 efflux ABC transporter YojI
* * ÷ NZ_CP009273 2368314 2368347 34 3 [1] [1] 4 menE o‑succinylbenzoate‑‑CoA ligase
* * ÷ NZ_CP009273 2492212 2492224 13 2 [1] [1] 2 ypdA two‑component system sensor histidine kinase YpdA
* * ÷ NZ_CP009273 2494575 2494598 24 2 [1] [1] 2 [ypdB] [ypdB]
* * ÷ NZ_CP009273 2537216 2537242 27 3 [1] [1] 2 ucpA SDR family oxidoreductase UcpA
* * ÷ NZ_CP009273 2563817 2563849 33 2 [1] [1] 2 eutE aldehyde dehydrogenase EutE
* * ÷ NZ_CP009273 2564138 2564192 55 2 [1] [1] 2 eutE aldehyde dehydrogenase EutE
* * ÷ NZ_CP009273 2623471 2623491 21 2 [1] [1] 3 yfgH glycine zipper 2TM domain‑containing protein
* * ÷ NZ_CP009273 2696967 2696987 21 2 [1] [1] 2 rnc ribonuclease III
* * ÷ NZ_CP009273 2823962 2824004 43 3 [1] [1] 2 gutQ arabinose‑5‑phosphate isomerase GutQ
* * ÷ NZ_CP009273 3765831 3765843 13 2 [1] [1] 3 mtlA PTS mannitol transporter subunit IICBA
* * ÷ NZ_CP009273 4085336 4085377 42 2 [1] [1] 2 frvB PTS fructose‑like transporter subunit IIBC
* * ÷ NZ_CP009273 4532470 4532484 15 2 [1] [0] 2 fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* * ÷ NZ_CP009273 4570849 4570854 6 2 [0] [1] 3 hsdS type I restriction‑modification system specificity subunit

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)13 (1.030) 10/172 0.3 54.2% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 11 (0.870)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 1391494NA (NA)9 (0.720) 7/170 0.5 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)21 (1.660) 14/172 0.1 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 4532499 =2 (0.160)5 (0.440) 5/154 0.6 40.3% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 13 (1.150)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA