breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 287 Δ1 bp 5.1% intergenic (+32/‑50) thrL → / → thrA thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1
RA 293:1 +G 5.2% intergenic (+38/‑44) thrL → / → thrA thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1
RA 9,907 A→C 6.2% intergenic (+14/+21) mog → / ← satP molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter
RA 9,908 G→T 6.1% intergenic (+15/+20) mog → / ← satP molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter
RA 151,688 C→G 9.6% V182L (GTA→CTA)  yadL ← putative fimbrial‑like adhesin protein
RA 164,581 A→C 20.4% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 164,613 T→A 11.2% intergenic (+79/‑117) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 164,620 C→T 6.6% intergenic (+86/‑110) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 164,621 A→G 6.7% intergenic (+87/‑109) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 226,978 A→G 5.2% noncoding (1220/2904 nt) rrlH → 23S ribosomal RNA of rrnH operon
RA 238,535 G→T 6.1% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 349,717 T→C 6.4% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 357,775 A→T 6.4% intergenic (+321/+16) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 357,778 C→T 5.2% intergenic (+324/+13) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 518,334 T→G 5.8% intergenic (‑55/+6) ybbN ← / ← ybbO DnaK co‑chaperone, thioredoxin‑like protein/putative oxidoreductase
RA 572,115 C→A 6.2% A221D (GCT→GAT)  ybcM → DLP12 prophage; putative DNA‑binding transcriptional regulator
RA 580,727 G→T 9.1% intergenic (‑282/‑107) ylcI ← / → nohD DUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein
RA 659,204 C→T 5.4% intergenic (+54/+47) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,205 A→T 5.6% intergenic (+55/+46) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,206 A→T 5.6% intergenic (+56/+45) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,207 A→G 5.6% intergenic (+57/+44) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 708,015 T→A 5.3% intergenic (+258/‑319) glnS → / → chiP glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
RA 777,208 A→C 8.3% K289N (AAA→AAC tolA → membrane anchored protein in TolA‑TolQ‑TolR complex
RA 857,576 T→C 5.0% intergenic (+21/+220) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 857,578 C→G 5.6% intergenic (+23/+218) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 857,580 G→A 5.0% intergenic (+25/+216) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 863,966 A→C 5.7% T109P (ACC→CCC)  fsaA → fructose‑6‑phosphate aldolase 1
RA 1,286,965 A→T 7.6% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,351,227 A→C 5.5% intergenic (‑188/+180) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,351,228 G→C 9.7% intergenic (‑189/+179) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,567,467 G→T 5.1% intergenic (‑327/+37) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
RA 1,708,709 T→A 5.4% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,814 A→T 8.4% A632A (GCA→GCT rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,720,364 G→T 5.8% intergenic (+21/+26) slyB → / ← slyA outer membrane lipoprotein/global transcriptional regulator
RA 1,864,758 G→A 5.7% intergenic (+24/+24) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,864,763 A→T 5.7% intergenic (+29/+19) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,864,768 T→C 6.8% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,008,698 T→G 5.1% I141S (ATT→AGT)  yedE → UPF0394 family sulphur transport domain‑containing inner membrane protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,177,229 G→T 6.5% intergenic (‑25/+283) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,177,234 C→A 13.8% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,177,245 G→T 12.4% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,229,177 A→C 14.2% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C
RA 2,304,649 C→T 11.0% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,688 A→G 8.8% intergenic (+295/+420) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,393,847 A→C 9.2% D400E (GAT→GAG nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L
RA 2,411,241 C→A 7.0% A38S (GCA→TCA)  yfbS ← putative transporter
RA 2,462,677 A→T 5.2% intergenic (+31/‑335) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,462,679 A→T 5.1% intergenic (+33/‑333) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,470,427 C→A 18.8% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,470,431 G→T 11.5% R434I (AGA→ATA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,470,436 C→A 15.8% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,725,832 G→T 6.4% intergenic (‑86/+237) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,731,580 C→T 5.0% intergenic (‑423/+20) rrsG ← / ← clpB 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone
RA 2,731,582 A→C 5.0% intergenic (‑425/+18) rrsG ← / ← clpB 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone
RA 2,731,583 A→G 5.0% intergenic (‑426/+17) rrsG ← / ← clpB 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone
RA 3,046,050 T→G 6.7% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,046,070 T→A 10.9% intergenic (‑169/+98) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,054,888 A→T 7.5% V186E (GTA→GAA)  ygfB ← UPF0149 family protein
RA 3,067,320 A→T 5.0% intergenic (‑147/+20) ygfI ← / ← yggE putative DNA‑binding transcriptional regulator/oxidative stress defense protein
RA 3,087,396 T→G 5.1% F231V (TTC→GTC)  metK → S‑adenosylmethionine synthetase
RA 3,156,591 T→G 5.2% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,239,609 T→G 8.4% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,263,518 T→A 6.8% N48I (AAT→ATT)  tdcD ← propionate kinase/acetate kinase C, anaerobic
RA 3,392,328 A→C 5.2% intergenic (‑300/+130) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein
RA 3,423,390 C→T 5.5% intergenic (+196/+33) yhdZ → / ← rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
RA 3,423,396 T→G 5.7% intergenic (+202/+27) yhdZ → / ← rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
RA 3,458,110 C→A 100% T578T (ACC→ACA gspD → general secretory pathway component, cryptic
RA 3,482,325 G→A 100% R346H (CGC→CAC)  yheS → ABC‑F family protein predicted regulatory ATPase
RA 3,491,722 T→A 7.4% intergenic (‑102/+3) yhfG ← / ← ppiA putative antitoxin for Fic/peptidyl‑prolyl cis‑trans isomerase A (rotamase A)
RA 3,504,855 T→G 6.2% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,504,873 T→A 14.1% intergenic (+90/+62) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,608,695 C→G 13.3% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,657,599 T→A 5.3% intergenic (+32/+387) hdeD → / ← arrS acid‑resistance membrane protein/antisense sRNA ArrS, function unknown
RA 3,657,601 G→C 5.2% intergenic (+34/+385) hdeD → / ← arrS acid‑resistance membrane protein/antisense sRNA ArrS, function unknown
RA 3,657,603 T→A 5.3% intergenic (+36/+383) hdeD → / ← arrS acid‑resistance membrane protein/antisense sRNA ArrS, function unknown
RA 3,781,022 T→G 7.3% intergenic (+5/‑193) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,051 C→G 6.1% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,828,980 A→C 6.8% Q12H (CAA→CAC xanP → xanthine permease
RA 3,908,512 A→T 6.9% intergenic (‑146/+37) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease
RA 3,908,514 A→T 8.9% intergenic (‑148/+35) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease
RA 3,959,457 T→G 6.5% intergenic (+12/+75) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,483 G→C 6.9% intergenic (+38/+49) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,515 T→A 7.7% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,984,542 T→A 6.3% Q489L (CAG→CTG)  aslA ← putative Ser‑type periplasmic non‑aryl sulfatase
RA 4,005,308 A→T 5.6% A149A (GCA→GCT pldA → outer membrane phospholipase A
RA 4,015,093 G→T 10.7% D681Y (GAT→TAT)  metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
RA 4,070,471 A→T 7.0% intergenic (‑70/+44) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase
RA 4,086,948 C→A 6.1% intergenic (+99/‑54) fdhD → / → yiiG formate dehydrogenase formation protein/DUF3829 family lipoprotein
RA 4,296,060 C→T 33.2% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,155 T→C 6.0% intergenic (+361/+281) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,303 A→G 7.1% intergenic (+509/+133) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,473,927 C→A 100% A43S (GCC→TCC)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,596,781 A→T 5.6% K264N (AAA→AAT lgoD → L‑galactonate oxidoreductase; L‑gulonate oxidoreductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423709–3424234 3424572–3424238 5–864 327 [323] [323] 329 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 16653NA (NA)5 (0.010) 5/262 NT NA noncoding (1267/1345 nt) IS186 repeat region
?NC_000913 = 16691 NA (NA)noncoding (1305/1345 nt) IS186 repeat region
* ? NC_000913 = 227039379 (0.690)41 (0.080) 10/264 NT 10.2% noncoding (1281/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 2727897 = NA (NA)noncoding (1288/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
* ? NC_000913 273985 =NA (NA)4 (0.010) 3/264 NT NA noncoding (1165/1195 nt) IS5 repeat region
?NC_000913 274026 = NA (NA)noncoding (1124/1195 nt) IS5 repeat region
* ? NC_000913 274038 =NA (NA)5 (0.010) 4/264 NT NA noncoding (1112/1195 nt) IS5 repeat region
?NC_000913 274083 = NA (NA)noncoding (1067/1195 nt) IS5 repeat region
* ? NC_000913 274347 =NA (NA)7 (0.010) 5/262 NT NA noncoding (803/1195 nt) IS5 repeat region
?NC_000913 274410 = NA (NA)noncoding (740/1195 nt) IS5 repeat region
* ? NC_000913 274620 =NA (NA)8 (0.020) 7/258 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 = 381669NA (NA)9 (0.020) 6/262 NT NA noncoding (410/1331 nt) IS2 repeat region
?NC_000913 = 381695 NA (NA)noncoding (436/1331 nt) IS2 repeat region
* ? NC_000913 = 381689NA (NA)3 (0.010) 3/258 NT NA noncoding (430/1331 nt) IS2 repeat region
?NC_000913 = 381743 NA (NA)noncoding (484/1331 nt) IS2 repeat region
* ? NC_000913 781369 =427 (0.780)62 (0.120)
+GAAG
33/268 NT 13.5% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 389 (0.710)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 1207790 =250 (0.460)202 (0.420) 99/244 NT 45.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 263 (0.540)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805248 (0.450)244 (0.500) 120/244 NT 50.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 263 (0.540)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)415 (0.780) 167/268 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1552167NA (NA)12 (0.020) 9/262 NT NA noncoding (97/97 nt) RIP120 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP120 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 = 1552320 NA (NA)noncoding (72/97 nt) RIP121 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP121 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 = 2817942434 (0.790)25 (0.050) 18/236 NT 6.4% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?NC_000913 2818492 = 366 (0.780)noncoding (58/77 nt) argV tRNA‑Arg
* ? NC_000913 = 3620220NA (NA)11 (0.020) 7/264 NT 100% coding (1029/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3620255 0 (0.000)coding (1064/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 4128030NA (NA)12 (0.020) 5/262 NT NA noncoding (79/97 nt) RIP305 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP305 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 = 4128041 NA (NA)noncoding (90/97 nt) RIP305 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP305 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 4542682 =425 (0.770)25 (0.050) 20/258 NT 5.9% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 397 (0.770)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 = 4542690416 (0.760)27 (0.050) 22/258 NT 6.4% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 397 (0.770)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)