breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 35,814 Δ1 bp 32.7% intergenic (+45/+63) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,817 A→T 30.8% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,850 C→T 36.8% intergenic (+81/+27) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,858 T→A 38.3% intergenic (+89/+19) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,876 C→T 47.3% intergenic (+107/+1) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,913 C→G 29.3% S213S (TCG→TCC USA300HOU_0026 ← IS431mec transposase
RA 35,940 G→A 31.2% R204R (CGC→CGT USA300HOU_0026 ← IS431mec transposase
RA 35,970 T→C 56.4% E194E (GAA→GAG USA300HOU_0026 ← IS431mec transposase
RA 35,997 T→C 33.2% T185T (ACA→ACG USA300HOU_0026 ← IS431mec transposase
RA 36,015 C→T 32.5% R179R (AGG→AGA USA300HOU_0026 ← IS431mec transposase
RA 36,087 A→G 58.4% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase
RA 36,119 C→T 32.2% V145I (GTA→ATA)  USA300HOU_0026 ← IS431mec transposase
RA 36,124 G→A 58.1% A143V (GCT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,133 A→G 31.7% V140A (GTA→GCA)  USA300HOU_0026 ← IS431mec transposase
RA 36,161 T→C 32.5% I131V (ATT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,174 T→C 33.3% K126K (AAA→AAG USA300HOU_0026 ← IS431mec transposase
RA 36,269 C→T 61.3% A95T (GCA→ACA)  USA300HOU_0026 ← IS431mec transposase
RA 36,315 G→A 58.3% Y79Y (TAC→TAT USA300HOU_0026 ← IS431mec transposase
RA 36,378 C→T 90.3% L58L (TTG→TTA USA300HOU_0026 ← IS431mec transposase
RA 36,413 C→T 55.6% V47I (GTC→ATC)  USA300HOU_0026 ← IS431mec transposase
RA 36,444 C→T 50.9% R36R (AGG→AGA USA300HOU_0026 ← IS431mec transposase
RA 36,471 A→G 29.8% S27S (AGT→AGC USA300HOU_0026 ← IS431mec transposase
RA 36,479 T→C 12.0% T25A (ACA→GCA)  USA300HOU_0026 ← IS431mec transposase
RA 36,501 G→A 5.2% A17A (GCC→GCT USA300HOU_0026 ← IS431mec transposase
RA 36,581 C→T 33.6% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein
RA 76,180 C→T 37.7% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,185 G→A 20.3% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,265 C→T 30.7% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,295 T→C 60.6% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,322 G→A 60.2% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,353 G→A 54.6% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,889 G→A 9.1% intergenic (+947/‑187) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 110,660 T→A 13.5% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,330 C→A 6.5% Q134K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,512 A→G 30.1% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 32.0% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,560 T→C 9.8% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 156,495 C→G 4.3% A81P (GCC→CCC)  USA300HOU_0147 ← transcriptional regulator
RA 512,643 A→G 54.1% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 587,957 G→T 11.4% A621S (GCG→TCG)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 787,902 G→A 5.5% R23H (CGC→CAC)  pabC → possible aminodeoxychorismate lyase
RA 816,291 A→G 4.6% I11T (ATC→ACC)  USA300HOU_0763 ← hypothetical protein
RA 821,301 T→C 5.5% P346P (CCA→CCG pepT ← M20 family peptidase T
RA 937,148 T→C 5.0% intergenic (+55/‑1962) USA300HOU_0902 → / → USA300HOU_0903 NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
RA 937,158 T→A 5.3% intergenic (+65/‑1952) USA300HOU_0902 → / → USA300HOU_0903 NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
RA 937,168 G→A 5.3% intergenic (+75/‑1942) USA300HOU_0902 → / → USA300HOU_0903 NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
RA 1,035,144 C→T 6.1% intergenic (‑1561/‑6145) USA300HOU_0983 ← / → USA300HOU_0984 competence protein/glycosyltransferase
RA 1,117,630 C→T 8.0% intergenic (+3416/‑104) USA300HOU_1046 → / → USA300HOU_1047 hypothetical protein/GTP‑binding protein TypA
RA 1,126,832 G→A 4.4% A238V (GCG→GTG)  ctaA ← cytochrome oxidase assembly protein
RA 1,141,228 G→A 11.3% E283K (GAA→AAA)  USA300HOU_1066 → hypothetical protein
RA 1,231,407 G→A 6.5% E18K (GAA→AAA)  USA300HOU_1156 → possible PP2C protein phosphatase
RA 1,400,393 C→T 6.1% P73L (CCA→CTA)  USA300HOU_1299 → ImpB/MucB/SamB family DNA‑damage repair protein
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,433,870 A→G 10.7% D287G (GAC→GGC)  dapA → dihydrodipicolinate synthase
RA 1,472,896 T→C 7.0% K9710E (AAG→GAG)  ebh ← extracellular matrix binding protein
RA 1,507,931 C→T 6.5% intergenic (‑356/+119) ald1 ← / ← USA300HOU_1377 alanine dehydrogenase/5'‑3' exonuclease
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,589,223 A→G 7.6% V753V (GTT→GTC USA300HOU_1459 ← hypothetical bacteriophage protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,673,843 T→C 6.1% K391K (AAA→AAG dnaG ← DNA primase
RA 1,685,490 A→G 4.7% intergenic (‑106/+187) rpsU ← / ← USA300HOU_1577 ribosomal protein S21/possible 2‑methylthioadenine synthase
RA 1,744,443 C→A 16.4% V77F (GTT→TTT)  apt ← adenine phosphoribosyltransferase
RA 1,744,446 G→A 7.6% P76S (CCT→TCT)  apt ← adenine phosphoribosyltransferase
RA 1,744,544 T→C 43.8% Y43C (TAC→TGC)  apt ← adenine phosphoribosyltransferase
RA 1,781,307 T→C 5.1% K471E (AAG→GAG)  lysP ← APC family amino acid‑polyamine‑organocation transporter
RA 1,815,180 T→C 8.7% E21G (GAA→GGA)  dnaE2 ← DNA‑directed DNA polymerase III alpha subunit
RA 1,929,796 T→C 56.6% intergenic (‑879/‑289) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,797 G→A 56.7% intergenic (‑880/‑288) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,799 C→A 55.3% intergenic (‑882/‑286) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,804 A→G 56.6% intergenic (‑887/‑281) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,812 G→A 66.7% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,842 Δ1 bp 100% intergenic (‑925/‑243) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,843 Δ1 bp 70.5% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,848 C→A 71.6% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,861 T→C 73.5% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,863:1 +T 51.1% intergenic (‑946/‑222) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,864 A→T 21.4% intergenic (‑947/‑221) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865 A→C 52.7% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865:1 +C 13.0% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866 G→T 70.7% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866:1 +T 4.3% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,867 G→T 72.7% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,870 A→C 72.9% intergenic (‑953/‑215) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,872 G→A 72.1% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,873 C→A 72.3% intergenic (‑956/‑212) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,875 T→C 72.6% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,917 C→T 64.2% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,918 A→G 63.7% intergenic (‑1001/‑167) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,925 T→A 61.7% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,928 T→A 61.2% intergenic (‑1011/‑157) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,930 T→A 60.7% intergenic (‑1013/‑155) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,941 A→T 50.5% intergenic (‑1024/‑144) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,946 G→A 47.5% intergenic (‑1029/‑139) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,084,760 G→T 8.5% intergenic (‑22/‑936) mapW2 ← / → USA300HOU_1944 cell surface protein MapW2/hypothetical protein
RA 2,150,242 C→G 6.1% H208D (CAT→GAT)  agrA → accessory gene regulator protein A
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,305,823 C→T 12.2% intergenic (‑126/‑51) USA300HOU_2163 ← / → USA300HOU_2164 hypothetical membrane protein/hypothetical protein
RA 2,331,676 A→G 5.4% Y81Y (TAT→TAC lacC ← tagatose‑6‑phosphate kinase
RA 2,337,959 C→T 3.8% intergenic (‑125/‑119) USA300HOU_2195 ← / → USA300HOU_2196 possible MerR family transcriptional regulator/hypothetical protein
RA 2,373,506 A→T 15.7% N90K (AAT→AAA USA300HOU_2234 ← NCS2 family nucleobase:cation symporter‑2
RA 2,442,876 T→C 4.4% T164T (ACA→ACG USA300HOU_2305 ← hypothetical protein
RA 2,504,989 T→C 8.8% intergenic (+293/+111) USA300HOU_2365 → / ← gltT hypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,540,782 G→A 7.4% E118K (GAA→AAA)  USA300HOU_2401 → immunoglobulin G‑binding protein SBI
RA 2,605,313 T→G 4.0% intergenic (‑107/+345) USA300HOU_2465 ← / ← USA300HOU_2466 possible decarboxylase/hypothetical protein
RA 2,699,344 A→T 4.8% intergenic (+248/‑43) copA → / → merP P‑ATPase superfamily P‑type ATPase copper (Cu2+) transporter/MerTP family mercury (Hg2+) permease, binding protein MerP
RA 2,771,843 C→A 9.0% F214L (TTC→TTA USA300HOU_2626 → alkaline phosphatase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 37094 =98 (1.250)9 (0.120) 4/240 NT 10.1% coding (21/231 nt) USA300HOU_0028 hypothetical protein
?CP000730 37188 = 69 (0.960)coding (115/231 nt) USA300HOU_0028 hypothetical protein
* ? CP000730 512620 =81 (1.040)68 (1.260)
+40 bp
44/180 NT 54.9% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 1822717 =60 (0.770)3 (0.040) 3/238 NT 5.5% intergenic (+38/+208) USA300HOU_1697/ackA possible universal stress protein/acetate kinase
?CP000730 1822745 = 49 (0.680)intergenic (+66/+180) USA300HOU_1697/ackA possible universal stress protein/acetate kinase
* ? CP000730 2085296 =54 (0.690)13 (0.190) 12/232 NT 18.6% intergenic (‑558/‑400) mapW2/USA300HOU_1944 cell surface protein MapW2/hypothetical protein
?CP000730 = 2128343 66 (0.950)intergenic (+57/+1062) int/USA300HOU_2011 bacteriophage integrase/possible leukocidin subunit