breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 15,571 T→C 100% L43L (TTG→CTG)  EK12AG100_RS00080 → IS4‑like element IS421 family transposase
JC 61,414 Δ168 bp 100% [rapA] [rapA]
RA 66,696 T→C 78.5% Q8R (CAG→CGG)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase
RA 70,457 G→T 76.8% intergenic (‑241/‑98) araB ← / → araC ribulokinase/arabinose operon transcriptional regulator AraC
RA 246,915 T→G 73.1% S12S (TCT→TCG EK12AG100_RS01160 → C40 family peptidase
RA 283,927 G→T 83.8% A445A (GCG→GCT EK12AG100_RS01390 → xylonate dehydratase YagF
RA 312,935 A→G 75.8% intergenic (‑301/+173) EK12AG100_RS01540 ← / ← EK12AG100_RS24935 hypothetical protein/oxidoreductase
RA 339,234 T→C 22.7% V17A (GTC→GCC)  EK12AG100_RS01670 → hypothetical protein
RA 339,240 T→C 22.9% I19T (ATT→ACT)  EK12AG100_RS01670 → hypothetical protein
RA 339,257 A→G 27.4% N25D (AAC→GAC)  EK12AG100_RS01670 → hypothetical protein
RA 339,278 A→G 28.0% M32V (ATG→GTG)  EK12AG100_RS01670 → hypothetical protein
RA 339,285 A→G 29.3% Q34R (CAA→CGA)  EK12AG100_RS01670 → hypothetical protein
RA 339,327 T→C 17.7% V48A (GTC→GCC)  EK12AG100_RS01670 → hypothetical protein
RA 339,333 T→C 17.0% I50T (ATT→ACT)  EK12AG100_RS01670 → hypothetical protein
JC 339,349 +186 bp 17.2% coding (165/204 nt) EK12AG100_RS01670 → hypothetical protein
RA 365,818 G→A 32.2% A331V (GCG→GTG)  EK12AG100_RS01780 ← DNA‑binding transcriptional repressor LacI
RA 384,896 A→C 100% G122G (GGA→GGC tauA → taurine ABC transporter substrate‑binding protein
RA 393,261 T→C 47.6% pseudogene (987/1443 nt) EK12AG100_RS01925 → autotransporter outer membrane beta‑barrel domain‑containing protein
RA 464,304 A→G 50.9% V181V (GTT→GTC queC ← 7‑cyano‑7‑deazaguanine synthase QueC
RA 472,148 A→G 45.7% G84G (GGA→GGG glnK → P‑II family nitrogen regulator
RA 494,921 T→C 54.7% I168T (ATC→ACC)  htpG → molecular chaperone HtpG
RA 502,728 C→T 52.9% intergenic (‑191/+47) EK12AG100_RS02465 ← / ← EK12AG100_RS02470 Kef family K(+) transporter/fosmidomycin MFS transporter
RA 534,041 A→G 58.5% D276G (GAT→GGT)  gcl → glyoxylate carboligase
RA 575,186 A→G 24.1% intergenic (‑134/‑83) EK12AG100_RS02845 ← / → rzoD IS5‑like element IS5 family transposase/prophage lysis lipoprotein RzoD
RA 575,273 G→T 9.9% R2L (CGA→CTA)  rzoD → prophage lysis lipoprotein RzoD
RA 575,369 C→G 74.3% P34R (CCG→CGG)  rzoD → prophage lysis lipoprotein RzoD
RA 575,513 T→C 79.3% intergenic (+62/+29) rzoD → / ← bor prophage lysis lipoprotein RzoD/serum resistance lipoprotein Bor
RA 575,516 C→A 77.8% intergenic (+65/+26) rzoD → / ← bor prophage lysis lipoprotein RzoD/serum resistance lipoprotein Bor
RA 575,547 G→T 79.7% Q97K (CAA→AAA)  bor ← serum resistance lipoprotein Bor
RA 575,671 G→A 86.6% A55A (GCC→GCT bor ← serum resistance lipoprotein Bor
RA 575,751 C→T 84.7% A29T (GCA→ACA)  bor ← serum resistance lipoprotein Bor
RA 575,753 G→T 83.6% P28Q (CCG→CAG)  bor ← serum resistance lipoprotein Bor
RA 575,773 C→T 83.4% T21T (ACG→ACA bor ← serum resistance lipoprotein Bor
RA 575,946 T→C 77.6% intergenic (‑111/+179) bor ← / ← EK12AG100_RS02865 serum resistance lipoprotein Bor/DUF1398 domain‑containing protein
RA 576,036:1 +A 100% intergenic (‑201/+89) bor ← / ← EK12AG100_RS02865 serum resistance lipoprotein Bor/DUF1398 domain‑containing protein
RA 576,068 C→T 85.0% intergenic (‑233/+57) bor ← / ← EK12AG100_RS02865 serum resistance lipoprotein Bor/DUF1398 domain‑containing protein
RA 576,075 C→T 84.6% intergenic (‑240/+50) bor ← / ← EK12AG100_RS02865 serum resistance lipoprotein Bor/DUF1398 domain‑containing protein
RA 576,087 G→A 85.5% intergenic (‑252/+38) bor ← / ← EK12AG100_RS02865 serum resistance lipoprotein Bor/DUF1398 domain‑containing protein
RA 576,212 A→G 84.3% H108H (CAT→CAC EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,226 C→T 85.5% V104I (GTT→ATT)  EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,326 G→A 86.2% N70N (AAC→AAT EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,398 G→A 88.9% I46I (ATC→ATT EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,443 G→A 89.6% N31N (AAC→AAT EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,481 C→T 90.3% D19N (GAC→AAC)  EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,592 T→C 82.0% intergenic (‑57/‑229) EK12AG100_RS02865 ← / → EK12AG100_RS02870 DUF1398 domain‑containing protein/hypothetical protein
RA 576,612 T→C 17.3% intergenic (‑77/‑209) EK12AG100_RS02865 ← / → EK12AG100_RS02870 DUF1398 domain‑containing protein/hypothetical protein
RA 576,658 C→A 79.6% intergenic (‑123/‑163) EK12AG100_RS02865 ← / → EK12AG100_RS02870 DUF1398 domain‑containing protein/hypothetical protein
RA 576,752 T→C 81.0% intergenic (‑217/‑69) EK12AG100_RS02865 ← / → EK12AG100_RS02870 DUF1398 domain‑containing protein/hypothetical protein
RA 576,961 A→G 90.7% R47R (AGA→AGG EK12AG100_RS02870 → hypothetical protein
RA 577,056 T→C 86.8% intergenic (+29/+136) EK12AG100_RS02870 → / ← EK12AG100_RS02875 hypothetical protein/YlcI/YnfO family protein
RA 577,060 A→G 13.0% intergenic (+33/+132) EK12AG100_RS02870 → / ← EK12AG100_RS02875 hypothetical protein/YlcI/YnfO family protein
RA 577,061 C→T 12.9% intergenic (+34/+131) EK12AG100_RS02870 → / ← EK12AG100_RS02875 hypothetical protein/YlcI/YnfO family protein
RA 577,243 T→C 82.8% R48R (AGA→AGG EK12AG100_RS02875 ← YlcI/YnfO family protein
RA 577,378 A→C 5.0% T3T (ACT→ACG EK12AG100_RS02875 ← YlcI/YnfO family protein
RA 577,658 A→G 100% intergenic (‑272/‑117) EK12AG100_RS02875 ← / → EK12AG100_RS02885 YlcI/YnfO family protein/DNA‑packaging protein
RA 577,771 T→A 11.2% intergenic (‑385/‑4) EK12AG100_RS02875 ← / → EK12AG100_RS02885 YlcI/YnfO family protein/DNA‑packaging protein
RA 577,772 G→A 11.8% intergenic (‑386/‑3) EK12AG100_RS02875 ← / → EK12AG100_RS02885 YlcI/YnfO family protein/DNA‑packaging protein
RA 577,983 G→T 72.2% R70L (CGG→CTG)  EK12AG100_RS02885 → DNA‑packaging protein
RA 577,997 G→A 72.5% A75T (GCA→ACA)  EK12AG100_RS02885 → DNA‑packaging protein
RA 578,014 G→A 30.9% G80G (GGG→GGA EK12AG100_RS02885 → DNA‑packaging protein
RA 578,059 C→T 76.7% D95D (GAC→GAT EK12AG100_RS02885 → DNA‑packaging protein
RA 578,068 A→G 75.0% E98E (GAA→GAG EK12AG100_RS02885 → DNA‑packaging protein
RA 578,074 A→G 25.0% K100K (AAA→AAG EK12AG100_RS02885 → DNA‑packaging protein
RA 578,126 T→C 77.3% L118L (TTG→CTG)  EK12AG100_RS02885 → DNA‑packaging protein
RA 578,168 C→A 79.4% L132I (CTC→ATC)  EK12AG100_RS02885 → DNA‑packaging protein
RA 579,538 T→C 82.9% N61S (AAT→AGT)  EK12AG100_RS02895 ← class I SAM‑dependent methyltransferase
RA 691,882 A→G 77.1% L5L (CTT→CTC miaB ← tRNA (N6‑isopentenyl adenosine(37)‑C2)‑methylthiotransferase MiaB
RA 693,411 T→C 82.7% intergenic (+194/+72) ubiF → / ← EK12AG100_RS03430 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase/tRNA‑Gln
RA 701,954 T→C 87.5% L357S (TTG→TCG)  nagE → PTS N‑acetyl glucosamine transporter subunit IIABC
RA 705,715 A→G 77.2% A147A (GCA→GCG chiP → chitoporin
RA 718,545 A→G 80.6% C44R (TGT→CGT)  kdpE ← two‑component system response regulator KdpE
RA 777,565 T→C 82.5% noncoding (71/76 nt) EK12AG100_RS03840 → tRNA‑Lys
RA 786,521 A→C 84.8% pseudogene (400/1149 nt) galK ← galactokinase
RA 841,645 A→T 64.7% G7G (GGA→GGT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,648 C→A 66.4% V8V (GTC→GTA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,687 C→A 70.3% T21T (ACC→ACA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,690 T→C 70.6% I22I (ATT→ATC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,702 C→A 70.3% A26A (GCC→GCA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,704 G→A 70.5% R27K (AGA→AAA)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,736 G→C 70.1% V38L (GTG→CTG)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,740 G→C 70.5% G39A (GGC→GCC)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,747 G→A 70.5% E41E (GAG→GAA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,780 T→C 74.7% D52D (GAT→GAC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,783 G→T 75.4% V53V (GTG→GTT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,801 T→C 72.2% S59S (AGT→AGC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,804 C→T 71.4% V60V (GTC→GTT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,807 C→T 71.6% I61I (ATC→ATT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,811 C→T 70.4% Q63* (CAG→TAG) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,812 A→T 69.8% Q63L (CAG→CTG) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,816 T→G 73.3% V64V (GTT→GTG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,819 C→A 72.9% D65E (GAC→GAA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,822 T→A 72.0% G66G (GGT→GGA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,825 T→C 73.3% F67F (TTT→TTC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,828 A→G 72.8% P68P (CCA→CCG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,831 A→G 72.9% P69P (CCA→CCG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,834 G→A 72.8% S70S (TCG→TCA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,837 C→T 72.9% H71H (CAC→CAT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,849 C→T 71.8% I75I (ATC→ATT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,867 A→T 67.3% S81S (TCA→TCT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,873 G→C 66.7% P83P (CCG→CCC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,876 G→T 67.4% G84G (GGG→GGT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,895 T→G 64.9% C91G (TGT→GGT)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,921 G→T 62.6% R99R (CGG→CGT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,929 T→C 63.8% V102A (GTG→GCG) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,930 G→A 63.8% V102V (GTG→GTA) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,936 T→C 64.5% R104R (CGT→CGC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,940 C→T 64.6% L106F (CTT→TTT)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,980 T→C 70.2% V119A (GTG→GCG)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,992 G→A 68.9% S123N (AGT→AAT) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 841,993 T→C 68.9% S123S (AGT→AGC) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,001 A→C 71.2% D126A (GAC→GCC)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,011 A→G 71.7% K129K (AAA→AAG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,018 G→A 71.1% G132S (GGC→AGC) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,020 C→T 71.1% G132G (GGC→GGT) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,029 T→C 72.7% S135S (AGT→AGC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,030 G→A 74.4% A136T (GCA→ACA)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,038 T→C 72.5% A138A (GCT→GCC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,039 G→C 72.5% A139P (GCT→CCT) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,040 C→G 72.5% A139G (GCT→GGT) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,042 C→G 72.5% Q140E (CAG→GAG)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,046 T→C 70.9% V141A (GTC→GCC) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,047 C→G 70.8% V141V (GTC→GTG) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,050 G→A 67.5% A142A (GCG→GCA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,051 G→A 67.5% A143T (GCC→ACC)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,055 T→A 67.9% L144H (CTT→CAT)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,058 T→C 67.8% V145A (GTG→GCG)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,060 A→G 68.2% T146A (ACT→GCT)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,068 A→T 68.2% A148A (GCA→GCT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,069 A→G 68.3% T149A (ACT→GCT) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,071 T→G 68.4% T149T (ACT→ACG) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,086 C→A 70.5% A154A (GCC→GCA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,098 C→A 73.1% S158S (TCC→TCA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,110 T→C 69.8% A162A (GCT→GCC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,113 A→G 69.8% A163A (GCA→GCG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,119 A→G 69.8% S165S (TCA→TCG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,126 G→T 69.8% E168* (GAA→TAA) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,127 A→C 70.9% E168A (GAA→GCA) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,134 C→T 72.9% S170S (TCC→TCT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,138 G→A 73.2% G172S (GGC→AGC)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,145 A→G 74.6% E174G (GAA→GGA)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,147 G→A 76.3% A175T (GCG→ACG)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,156 G→A 74.8% A178T (GCA→ACA)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,173 G→A 77.8% A183A (GCG→GCA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,174 G→T 75.7% E184* (GAA→TAA) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,175 A→C 76.5% E184A (GAA→GCA) ‡ EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,185 C→T 77.1% A187A (GCC→GCT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,188 A→C 77.2% A188A (GCA→GCC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,191 C→T 77.0% A189A (GCC→GCT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,208 A→G 74.2% N195S (AAC→AGC)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,258 G→T 75.2% A212S (GCA→TCA)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,268 A→T 73.4% Q215L (CAA→CTA)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,278 C→A 73.1% A218A (GCC→GCA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,284 G→A 72.8% T220T (ACG→ACA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,290 C→A 73.4% A222A (GCC→GCA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,300 G→A 75.0% A226T (GCC→ACC)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,308 A→G 75.9% K228K (AAA→AAG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,311 G→A 75.9% A229A (GCG→GCA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,317 G→A 75.9% E231E (GAG→GAA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,320 C→T 76.0% A232A (GCC→GCT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,323 C→G 76.0% A233A (GCC→GCG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,326 T→C 75.9% T234T (ACT→ACC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,329 A→G 74.3% S235S (TCA→TCG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,332 A→C 75.9% A236A (GCA→GCC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,335 A→G 78.2% R237R (CGA→CGG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,343 T→C 76.8% V240A (GTG→GCG)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,356 G→A 74.8% E244E (GAG→GAA EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,359 A→G 74.1% A245A (GCA→GCG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,392 T→C 73.5% S256S (AGT→AGC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,396 G→A 72.1% G258S (GGT→AGT)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,399 C→A 71.9% R259S (CGT→AGT)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,427 A→G 62.9% E268G (GAA→GGA)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,434 T→C 61.8% S270S (TCT→TCC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,437 C→G 56.0% A271A (GCC→GCG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,439 G→A 58.8% R272K (AGG→AAG)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,464 T→C 50.5% N280N (AAT→AAC EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,473 A→T 46.2% S283S (TCA→TCT EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,482 A→G 50.0% T286T (ACA→ACG EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,490 A→G 45.0% E289G (GAA→GGA)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,493 G→A 38.9% R290Q (CGG→CAG)  EK12AG100_RS23550 → short‑chain dehydrogenase
RA 842,503 T→G 35.3% S293S (TCT→TCG EK12AG100_RS23550 → short‑chain dehydrogenase
JC 932,107 Δ168 bp 100% [EK12AG100_RS04715] [EK12AG100_RS04715]
RA 1,011,377 A→G 77.3% N158D (AAT→GAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,015,338 A→C 16.2% L202V (TTA→GTA)  elyC ← envelope biogenesis factor ElyC
RA 1,082,666 T→G 82.3% L277L (CTT→CTG EK12AG100_RS05400 → cytochrome d ubiquinol oxidase subunit II
RA 1,104,475 A→G 85.2% N46S (AAC→AGC)  torD → molecular chaperone TorD
RA 1,123,847 G→A 100% intergenic (+494/‑49) putP → / → EK12AG100_RS05615 sodium/proline symporter PutP/FTR1 family protein
RA 1,154,267 T→C 85.2% L289L (TTG→CTG) ‡ mdoH → glucans biosynthesis glucosyltransferase MdoH
RA 1,154,268 T→C 85.1% L289S (TTG→TCG) ‡ mdoH → glucans biosynthesis glucosyltransferase MdoH
RA 1,212,376 G→A 82.8% P179L (CCG→CTG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,232,520 G→A 79.0% T156T (ACC→ACT phoP ← two‑component system response regulator PhoP
RA 1,238,760 T→C 78.6% H366H (CAT→CAC EK12AG100_RS06230 → NADP‑dependent isocitrate dehydrogenase
RA 1,238,772 T→C 76.2% T370T (ACT→ACC EK12AG100_RS06230 → NADP‑dependent isocitrate dehydrogenase
RA 1,238,785 C→T 73.3% L375L (CTG→TTG) ‡ EK12AG100_RS06230 → NADP‑dependent isocitrate dehydrogenase
RA 1,238,787 G→A 100% L375L (CTG→CTA) ‡ EK12AG100_RS06230 → NADP‑dependent isocitrate dehydrogenase
RA 1,297,205 G→A 9.6% T9I (ACT→ATT)  EK12AG100_RS06550 ← small toxic polypeptide LdrB
RA 1,328,213 T→A 85.1% Y271N (TAT→AAT)  oppA → oligopeptide ABC transporter substrate‑binding protein OppA
RA 1,332,957 G→T 84.6% A325S (GCC→TCC)  oppF → murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF
RA 1,334,905 C→T 85.8% intergenic (‑39/‑104) cls ← / → EK12AG100_RS25580 cardiolipin synthase/YciY family protein
RA 1,357,280 A→T 86.6% A4A (GCA→GCT topA → type I DNA topoisomerase
RA 1,357,281 T→C 87.2% F5L (TTT→CTT)  topA → type I DNA topoisomerase
RA 1,363,615 A→G 100% S522G (AGC→GGC)  acnA → aconitate hydratase AcnA
RA 1,385,080 C→T 78.6% C110Y (TGT→TAT)  puuP ← putrescine/proton symporter PuuP
RA 1,469,930 T→C 13.2% pseudogene (885/2526 nt) EK12AG100_RS07400 → autotransporter domain‑containing protein
RA 1,469,934 A→C 6.8% pseudogene (889/2526 nt) EK12AG100_RS07400 → autotransporter domain‑containing protein
RA 1,518,134 A→C 85.7% E217A (GAG→GCG)  EK12AG100_RS07620 → ABC transporter permease
RA 1,595,365 A→C 82.9% pseudogene (466/1323 nt) hipA ← type II toxin‑antitoxin system serine/threonine protein kinase toxin HipA
RA 1,632,901 T→C 81.2% I11I (ATT→ATC ydfZ → putative selenium delivery protein YdfZ
RA 1,636,206 A→C 11.3% intergenic (‑267/‑338) EK12AG100_RS08145 ← / → EK12AG100_RS25635 MHS family MFS transporter/Rac prophage; protein YnaM
RA 1,636,208 G→A 11.3% intergenic (‑269/‑336) EK12AG100_RS08145 ← / → EK12AG100_RS25635 MHS family MFS transporter/Rac prophage; protein YnaM
RA 1,636,209 T→C 11.3% intergenic (‑270/‑335) EK12AG100_RS08145 ← / → EK12AG100_RS25635 MHS family MFS transporter/Rac prophage; protein YnaM
RA 1,636,213 T→A 12.2% intergenic (‑274/‑331) EK12AG100_RS08145 ← / → EK12AG100_RS25635 MHS family MFS transporter/Rac prophage; protein YnaM
RA 1,636,308 T→C 33.5% intergenic (‑369/‑236) EK12AG100_RS08145 ← / → EK12AG100_RS25635 MHS family MFS transporter/Rac prophage; protein YnaM
RA 1,636,366 A→G 40.3% intergenic (‑427/‑178) EK12AG100_RS08145 ← / → EK12AG100_RS25635 MHS family MFS transporter/Rac prophage; protein YnaM
RA 1,636,389 G→A 41.9% intergenic (‑450/‑155) EK12AG100_RS08145 ← / → EK12AG100_RS25635 MHS family MFS transporter/Rac prophage; protein YnaM
RA 1,636,424 T→C 45.8% intergenic (‑485/‑120) EK12AG100_RS08145 ← / → EK12AG100_RS25635 MHS family MFS transporter/Rac prophage; protein YnaM
RA 1,636,491 A→G 51.6% intergenic (‑552/‑53) EK12AG100_RS08145 ← / → EK12AG100_RS25635 MHS family MFS transporter/Rac prophage; protein YnaM
RA 1,636,567 A→T 52.6% T8T (ACA→ACT EK12AG100_RS25635 → Rac prophage; protein YnaM
RA 1,636,570 T→G 54.8% S9S (TCT→TCG EK12AG100_RS25635 → Rac prophage; protein YnaM
RA 1,636,603 C→T 55.7% A20A (GCC→GCT EK12AG100_RS25635 → Rac prophage; protein YnaM
RA 1,636,877 A→C 46.2% K51T (AAA→ACA)  EK12AG100_RS08150 → cold shock protein YdfK
RA 1,637,283 A→G 51.4% Q3R (CAA→CGA)  EK12AG100_RS08155 → recombinase family protein
RA 1,637,578 G→T 44.3% G101G (GGG→GGT EK12AG100_RS08155 → recombinase family protein
RA 1,637,722 A→T 39.0% P149P (CCA→CCT EK12AG100_RS08155 → recombinase family protein
RA 1,637,751 C→T 38.7% A159V (GCG→GTG)  EK12AG100_RS08155 → recombinase family protein
RA 1,638,293 C→T 34.0% D83N (GAC→AAC)  EK12AG100_RS08160 ← tail fiber assembly protein
RA 1,638,677 T→C 15.8% A275A (GCA→GCG EK12AG100_RS08165 ← tail fiber protein
RA 1,638,680 A→G 15.8% H274H (CAT→CAC EK12AG100_RS08165 ← tail fiber protein
RA 1,638,683 C→T 16.0% A273A (GCG→GCA EK12AG100_RS08165 ← tail fiber protein
RA 1,638,686 G→A 16.2% G272G (GGC→GGT EK12AG100_RS08165 ← tail fiber protein
RA 1,638,713 C→T 13.8% S263S (TCG→TCA EK12AG100_RS08165 ← tail fiber protein
RA 1,638,725 A→T 12.0% A259A (GCT→GCA EK12AG100_RS08165 ← tail fiber protein
RA 1,638,728 A→G 11.9% G258G (GGT→GGC EK12AG100_RS08165 ← tail fiber protein
RA 1,638,732 T→G 10.2% D257A (GAT→GCT)  EK12AG100_RS08165 ← tail fiber protein
RA 1,638,734 T→A 9.5% S256S (TCA→TCT EK12AG100_RS08165 ← tail fiber protein
RA 1,638,947 G→A 9.6% T185T (ACC→ACT EK12AG100_RS08165 ← tail fiber protein
RA 1,638,950 T→G 9.2% G184G (GGA→GGC EK12AG100_RS08165 ← tail fiber protein
RA 1,638,976 C→T 11.2% E176K (GAA→AAA)  EK12AG100_RS08165 ← tail fiber protein
RA 1,638,989 C→T 11.4% T171T (ACG→ACA EK12AG100_RS08165 ← tail fiber protein
RA 1,639,016 G→A 11.7% H162H (CAC→CAT EK12AG100_RS08165 ← tail fiber protein
RA 1,639,046 T→C 11.8% L152L (TTA→TTG EK12AG100_RS08165 ← tail fiber protein
RA 1,639,067 G→A 11.2% P145P (CCC→CCT EK12AG100_RS08165 ← tail fiber protein
RA 1,639,070 C→T 11.3% K144K (AAG→AAA EK12AG100_RS08165 ← tail fiber protein
RA 1,639,124 A→G 10.8% V126V (GTT→GTC) ‡ EK12AG100_RS08165 ← tail fiber protein
RA 1,639,125 A→G 10.7% V126A (GTT→GCT) ‡ EK12AG100_RS08165 ← tail fiber protein
RA 1,639,142 T→A 10.3% A120A (GCA→GCT EK12AG100_RS08165 ← tail fiber protein
RA 1,639,159 T→C 9.5% T115A (ACT→GCT)  EK12AG100_RS08165 ← tail fiber protein
RA 1,639,728 T→C 100% E98E (GAA→GAG EK12AG100_RS08170 ← DNA‑packaging protein
RA 1,639,737 G→A 24.1% D95D (GAC→GAT EK12AG100_RS08170 ← DNA‑packaging protein
RA 1,639,799 C→T 100% A75T (GCA→ACA)  EK12AG100_RS08170 ← DNA‑packaging protein
RA 1,639,813 C→A 11.8% R70L (CGG→CTG)  EK12AG100_RS08170 ← DNA‑packaging protein
RA 1,639,827 T→C 9.1% E65E (GAA→GAG EK12AG100_RS08170 ← DNA‑packaging protein
RA 1,640,036 A→C 93.4% intergenic (‑15/‑374) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,640,067 C→A 8.2% intergenic (‑46/‑343) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,640,077 T→C 8.9% intergenic (‑56/‑333) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,640,109 G→A 11.3% intergenic (‑88/‑301) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,640,138 T→C 86.4% intergenic (‑117/‑272) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,640,157 C→T 85.9% intergenic (‑136/‑253) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,647,333 T→A 78.8% Q209L (CAG→CTG)  EK12AG100_RS08235 ← DUF968 domain‑containing protein
RA 1,724,790 T→C 79.1% F38L (TTT→CTT)  EK12AG100_RS08655 → DUF1656 domain‑containing protein
RA 1,780,369 T→A 77.8% C377S (TGT→AGT)  EK12AG100_RS08915 → acyl CoA:acetate/3‑ketoacid CoA transferase
JC 1,870,601 Δ182 bp 100% [EK12AG100_RS09385] [EK12AG100_RS09385]
RA 1,898,675 C→T 86.4% P12L (CCC→CTC)  pabB → aminodeoxychorismate synthase component 1
JC 1,982,569 (ATTA)2→1 68.2% intergenic (‑536/‑117) flhD ← / → uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
RA 2,043,369 A→C 78.1% D319A (GAC→GCC)  msrP → protein‑methionine‑sulfoxide reductase catalytic subunit MsrP
RA 2,123,905 A→G 75.0% L196L (TTG→CTG)  EK12AG100_RS10745 ← UDP‑glucose‑‑undecaprenyl‑phosphate glucose‑1‑phosphate transferase
RA 2,145,534 A→G 83.7% L117L (CTT→CTC EK12AG100_RS10840 ← uridine kinase
RA 2,176,382 T→C 83.7% Q277Q (CAA→CAG EK12AG100_RS10970 ← galactitol permease IIC component
RA 2,236,543 T→C 82.5% pseudogene (10/240 nt) EK12AG100_RS11245 → DUF2542 family protein
JC 2,242,224 +58 bp 100% intergenic (‑1/+2) mglA ← / ← mglB galactose/methyl galactoside ABC transporter ATP‑binding protein MglA/galactose/glucose ABC transporter substrate‑binding protein MglB
RA 2,245,123 T→C 78.6% R241G (AGG→GGG)  EK12AG100_RS11285 ← DUF418 family protein
RA 2,250,010 C→A 79.1% D467Y (GAC→TAC)  lysP ← lysine‑specific permease
RA 2,393,995 A→G 81.2% F129F (TTT→TTC nuoN ← NADH‑quinone oxidoreductase subunit NuoN
RA 2,407,278 A→G 74.8% L71S (TTG→TCG)  EK12AG100_RS12000 ← NADH‑quinone oxidoreductase subunit NuoB
RA 2,455,265 A→G 77.9% pseudogene (2613/2646 nt) yfcU ← fimbrial usher protein YfcU
RA 2,455,266 A→G 77.8% pseudogene (2612/2646 nt) yfcU ← fimbrial usher protein YfcU
RA 2,473,702 A→T 7.8% pseudogene (12/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,706 A→T 7.0% pseudogene (16/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,708 G→T 6.1% pseudogene (18/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,744 G→C 13.9% pseudogene (54/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,750 C→T 14.4% pseudogene (60/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,753 G→A 14.4% pseudogene (63/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,767 T→C 15.7% pseudogene (77/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,777 T→C 18.7% pseudogene (87/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,780 A→G 19.3% pseudogene (90/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,801 G→A 20.7% pseudogene (111/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,811 A→G 20.7% pseudogene (121/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,849 A→G 20.8% pseudogene (159/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,855 T→C 21.2% pseudogene (165/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,858 G→A 21.6% pseudogene (168/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,861 G→T 21.7% pseudogene (171/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,864 G→A 22.2% pseudogene (174/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,869 T→C 22.5% pseudogene (179/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,879 G→A 22.5% pseudogene (189/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,882 C→A 22.6% pseudogene (192/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,883 T→A 22.7% pseudogene (193/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,886 C→A 23.1% pseudogene (196/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,895 A→G 23.6% pseudogene (205/291 nt) EK12AG100_RS12330 → tail fiber assembly protein
RA 2,473,977 A→C 17.7% pseudogene (287/291 nt)
T139T (ACT→ACG
EK12AG100_RS12330 →
EK12AG100_RS12335 ←
tail fiber assembly protein
tail fiber assembly protein
RA 2,474,007 A→T 13.2% T129T (ACT→ACA EK12AG100_RS12335 ← tail fiber assembly protein
RA 2,474,013 G→A 11.2% V127V (GTC→GTT EK12AG100_RS12335 ← tail fiber assembly protein
RA 2,474,019 C→T 9.3% E125E (GAG→GAA EK12AG100_RS12335 ← tail fiber assembly protein
RA 2,474,028 G→T 8.7% D122E (GAC→GAA EK12AG100_RS12335 ← tail fiber assembly protein
RA 2,484,484 G→A 82.4% S190L (TCA→TTA)  emrY ← multidrug efflux MFS transporter permease subunit EmrY
RA 2,501,268 C→A 84.7% intergenic (‑97/‑279) EK12AG100_RS12470 ← / → EK12AG100_RS12475 alanine transaminase/two‑component system sensor histidine kinase YpdA
RA 2,517,333 T→C 100% L43L (TTG→CTG)  EK12AG100_RS12545 → IS4‑like element IS421 family transposase
JC 2,523,883 Δ120 bp 89.6% [EK12AG100_RS12585] [EK12AG100_RS12585]
RA 2,549,680 A→T 78.9% E44D (GAA→GAT murP → PTS N‑acetylmuramic acid transporter subunit IIBC
JC 2,561,645 Δ90 bp 100% [eutA] [eutA]
RA 2,574,113 C→T 75.0% E147E (GAG→GAA EK12AG100_RS12855 ← NADP‑dependent oxaloacetate‑decarboxylating malate dehydrogenase
RA 2,583,288 T→C 80.0% V461A (GTA→GCA)  narQ → nitrate/nitrite two‑component system sensor histidine kinase NarQ
JC 2,694,580 Δ128 bp 100% [EK12AG100_RS25720] [EK12AG100_RS25720]
RA 2,722,371 T→C 9.3% intergenic (‑321/+4) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA 2,741,904 G→A 83.3% H107Y (CAC→TAC)  rimM ← ribosome maturation factor RimM
RA 2,771,251 A→G 74.7% S544G (AGT→GGT)  EK12AG100_RS13785 → hypothetical protein
RA 2,783,895 G→A 76.2% intergenic (+157/‑51) EK12AG100_RS25740 → / → EK12AG100_RS25255 alpha amylase/alpha amylase
RA 2,840,314 G→T 76.9% R67R (CGC→CGA hycG ← formate hydrogenlyase subunit HycG
RA 2,863,759 A→G 81.7% pseudogene (97/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 2,876,939 C→T 87.0% L138L (TTG→TTA cas5e ← type I‑E CRISPR‑associated protein Cas5/CasD
RA 2,923,076 A→C 82.4% G155G (GGA→GGC ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 3,021,900:1 +T 100% pseudogene (1246/1400 nt) xanQ → xanthine/proton symporter XanQ
RA 3,067,161 T→G 23.6% K129N (AAA→AAC fbaA ← class II fructose‑bisphosphate aldolase
RA 3,106,724 C→A 74.8% noncoding (56/76 nt) EK12AG100_RS15430 → tRNA‑Phe
RA 3,204,060:1 +G 80.0% pseudogene (177/605 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,272,414 T→A 78.3% D207E (GAT→GAA garD → galactarate dehydratase
RA 3,292,993 A→G 84.6% M25V (ATG→GTG)  yraP → divisome‑associated lipoprotein YraP
RA 3,326,255 T→G 78.7% Y261D (TAT→GAT)  dacB → serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA 3,373,942 T→C 72.9% intergenic (‑10/+385) sspA ← / ← rpsI stringent starvation protein A/30S ribosomal protein S9
RA 3,384,553 G→T 78.9% P50T (CCG→ACG)  aaeB ← p‑hydroxybenzoic acid efflux pump subunit AaeB
RA 3,408,201 T→G 73.2% pseudogene (37/885 nt) yhdJ → adenine‑specific DNA‑methyltransferase
RA 3,419,861 G→A 22.0% pseudogene (25/81 nt) EK12AG100_RS25785 → paraslipin
RA 3,419,868 T→C 21.9% pseudogene (32/81 nt) EK12AG100_RS25785 → paraslipin
RA 3,419,895 A→G 15.9% pseudogene (59/81 nt) EK12AG100_RS25785 → paraslipin
RA 3,419,910 C→T 9.2% pseudogene (74/81 nt) EK12AG100_RS25785 → paraslipin
RA 3,419,924 T→C 6.8% intergenic (+7/+13) EK12AG100_RS25785 → / ← rrf paraslipin/5S ribosomal RNA
RA 3,430,412 T→C 75.9% L71L (TTA→CTA)  EK12AG100_RS17055 → peptide deformylase
RA 3,482,687 A→G 77.1% Y19C (TAC→TGC)  crp → cAMP‑activated global transcriptional regulator CRP
RA 3,483,014 T→C 75.8% I128T (ATT→ACT)  crp → cAMP‑activated global transcriptional regulator CRP
RA 3,493,618 A→G 75.4% D35G (GAC→GGC)  nirC → nitrite transporter NirC
RA 3,503,761 T→C 78.5% N30D (AAC→GAC)  EK12AG100_RS17515 ← membrane protein
RA 3,507,978 T→C 82.5% V293V (GTA→GTG EK12AG100_RS17545 ← transcriptional regulator
RA 3,551,559 T→G 80.2% C655G (TGC→GGC) ‡ malT → HTH‑type transcriptional regulator MalT
RA 3,551,560 G→T 80.3% C655F (TGC→TTC) ‡ malT → HTH‑type transcriptional regulator MalT
RA 3,556,874 A→G 77.8% V82A (GTC→GCC)  EK12AG100_RS17745 ← DeoR/GlpR family transcriptional regulator
RA 3,643,483 T→G 78.0% H224Q (CAT→CAG gorA → glutathione‑disulfide reductase
RA 3,643,941 T→C 75.9% V377A (GTG→GCG)  gorA → glutathione‑disulfide reductase
RA 3,704,396 A→G 76.1% intergenic (‑178/+565) dppA ← / ← EK12AG100_RS23155 dipeptide ABC transporter substrate‑binding protein DppA/RtT sRNA
RA 3,704,460 A→C 73.6% intergenic (‑242/+501) dppA ← / ← EK12AG100_RS23155 dipeptide ABC transporter substrate‑binding protein DppA/RtT sRNA
RA 3,715,942 A→G 75.9% intergenic (‑385/‑49) EK12AG100_RS18455 ← / → EK12AG100_RS18460 DUF3053 domain‑containing protein/HTH‑type transcriptional regulator
RA 3,717,968 T→C 76.7% L259L (CTT→CTC EK12AG100_RS25320 → IS3‑like element IS150 family transposase
RA 3,721,689 G→T 73.4% A48E (GCG→GAG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,724,623:1 +C 100% coding (1261/1353 nt) xylB ← xylulokinase
RA 3,725,952 T→C 76.8% intergenic (‑69/+3) xylB ← / ← xylA xylulokinase/xylose isomerase
RA 3,725,953 T→C 76.8% intergenic (‑70/+2) xylB ← / ← xylA xylulokinase/xylose isomerase
RA 3,727,072 T→C 71.7% pseudogene (206/1323 nt) xylA ← xylose isomerase
RA 3,766,256 C→T 82.4% intergenic (+64/+499) EK12AG100_RS18690 → / ← EK12AG100_RS18695 hypothetical protein/HlyD family secretion protein
RA 3,767,051 T→C 83.2% M281V (ATG→GTG)  EK12AG100_RS18695 ← HlyD family secretion protein
RA 3,769,682:1 +G 77.6% pseudogene (890/1913 nt) EK12AG100_RS18705 → PTS mannitol transporter subunit IICBA
RA 3,769,687 T→C 78.2% pseudogene (895/1913 nt) EK12AG100_RS18705 → PTS mannitol transporter subunit IICBA
RA 3,799,456 T→G 73.2% K38N (AAA→AAC waaO ← lipopolysaccharide 3‑alpha‑galactosyltransferase
RA 3,812,391 Δ1 bp 100% coding (671/717 nt) EK12AG100_RS18930 ← ribonuclease PH
RA 3,813,679 T→C 80.5% T164T (ACT→ACC EK12AG100_RS18935 → YicC family protein
RA 3,862,813 G→A 71.6% intergenic (‑28/+168) EK12AG100_RS19170 ← / ← ibpB putative transporter/heat shock chaperone IbpB
RA 3,862,823 A→G 71.2% intergenic (‑38/+158) EK12AG100_RS19170 ← / ← ibpB putative transporter/heat shock chaperone IbpB
RA 3,894,419 A→G 83.9% I134M (ATA→ATG EK12AG100_RS19335 → hypothetical protein
RA 3,917,872 T→C 75.6% Q231R (CAG→CGG) ‡ atpB ← F0F1 ATP synthase subunit A
RA 3,917,873 G→A 75.6% Q231* (CAG→TAG) ‡ atpB ← F0F1 ATP synthase subunit A
RA 3,925,308 T→C 62.9% T270A (ACG→GCG)  viaA ← ATPase RavA stimulator ViaA
RA 3,925,375 T→C 66.3% T247T (ACA→ACG viaA ← ATPase RavA stimulator ViaA
JC 3,942,060 +39 bp 100% noncoding (1854/2886 nt) EK12AG100_RS19545 → 23S ribosomal RNA
RA 4,022,771 T→G 25.5% Y437* (TAT→TAG ubiD → 4‑hydroxy‑3‑polyprenylbenzoate decarboxylase
RA 4,033,969 A→T 16.7% intergenic (+161/‑14) EK12AG100_RS20005 → / → EK12AG100_RS20010 tRNA‑Ala/23S ribosomal RNA
RA 4,090,243 C→T 85.0% G168D (GGC→GAC)  rhaD ← rhamnulose‑1‑phosphate aldolase
RA 4,150,309 A→G 77.3% pseudogene (1012/1152 nt) argE ← acetylornithine deacetylase
RA 4,157,721 G→T 68.2% G43V (GGC→GTC)  fabR → HTH‑type transcriptional repressor FabR
RA 4,160,596 C→G 71.2% A162G (GCA→GGA)  btuB → TonB‑dependent vitamin B12 receptor BtuB
RA 4,168,395 C→A 26.3% intergenic (‑68/‑135) EK12AG100_RS25840 ← / → EK12AG100_RS20635 paraslipin/UDP‑N‑acetylmuramate dehydrogenase
RA 4,168,398 C→T 27.5% intergenic (‑71/‑132) EK12AG100_RS25840 ← / → EK12AG100_RS20635 paraslipin/UDP‑N‑acetylmuramate dehydrogenase
JC 4,168,400 +183 bp 27.4% intergenic (‑73/‑130) EK12AG100_RS25840 ← / → EK12AG100_RS20635 paraslipin/UDP‑N‑acetylmuramate dehydrogenase
RA 4,190,469 G→T 73.2% A70D (GCT→GAT)  thiE ← thiamine phosphate synthase
RA 4,212,143 T→G 65.7% Y65D (TAT→GAT)  aceB → malate synthase A
RA 4,220,111 T→C 20.0% intergenic (‑10/‑190) iclR ← / → metH glyoxylate bypass operon transcriptional repressor IclR/methionine synthase
RA 4,292,533 T→C 100% intergenic (+266/+376) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,292,741 C→T 65.8% intergenic (+474/+168) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,292,759 C→T 27.8% intergenic (+492/+150) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,292,776 A→G 14.6% intergenic (+509/+133) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,292,777 A→C 15.7% intergenic (+510/+132) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,292,853:1 +CG 100% intergenic (+586/+56) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,303,525 T→C 75.3% N250D (AAT→GAT)  alsK ← allose kinase
RA 4,303,694 C→T 81.2% L193L (CTG→CTA alsK ← allose kinase
RA 4,322,117 T→C 81.2% N33S (AAC→AGC)  yjdN ← VOC family metalloprotein YjdN
RA 4,328,363 T→C 72.2% L463L (CTT→CTC EK12AG100_RS21375 → glycine betaine/L‑proline transporter ProP
RA 4,434,364 T→C 77.0% pseudogene (184/939 nt) EK12AG100_RS21930 → hypothetical protein
RA 4,443,520 T→A 80.0% S979T (TCC→ACC)  tamB → autotransporter assembly complex protein TamB
RA 4,470,374 T→C 73.1% R52G (AGA→GGA)  bdcA ← SDR family oxidoreductase
RA 4,471,308 A→G 79.3% intergenic (+117/‑28) EK12AG100_RS22100 → / → EK12AG100_RS22105 TetR/AcrR family transcriptional regulator/YhcH/YjgK/YiaL family protein
RA 4,471,967 C→T 79.3% F19F (TTC→TTT EK12AG100_RS22110 → hypothetical protein
RA 4,473,079 A→G 83.6% N390S (AAC→AGC)  EK12AG100_RS22110 → hypothetical protein
RA 4,485,490 T→C 76.1% K350E (AAA→GAA)  EK12AG100_RS22160 ← DUF853 domain‑containing protein
RA 4,506,712 G→T 83.5% intergenic (+105/+452) EK12AG100_RS25865 → / ← EK12AG100_RS22290 hypothetical protein/Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE
RA 4,528,883 G→T 77.7% intergenic (‑99/+28) EK12AG100_RS25395 ← / ← EK12AG100_RS22405 hypothetical protein/class I SAM‑dependent methyltransferase
RA 4,543,075 T→C 84.3% I502I (ATT→ATC fimD → fimbrial biogenesis usher protein FimD
RA 4,581,954 G→A 77.5% Q427* (CAA→TAA)  hsdR ← type I restriction‑modification system endonuclease
RA 4,598,983 C→T 72.2% W117* (TGG→TAG)  EK12AG100_RS22750 ← threonine/serine exporter ThrE family protein
RA 4,613,143 G→T 80.2% L133I (CTA→ATA)  EK12AG100_RS22840 ← YjjI family glycine radical enzyme
RA 4,633,399 C→G 76.1% P77R (CCC→CGC)  creC → two‑component system sensor histidine kinase CreC
RA 4,634,714 A→G 87.4% L21L (TTA→TTG creD → cell envelope integrity protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_LN832404 804284 841603 37320 132 [26] [46] 48 EK12AG100_RS03995–EK12AG100_RS04290 EK12AG100_RS03995,EK12AG100_RS04000,EK12AG100_RS04005,EK12AG100_RS04010,EK12AG100_RS04015,EK12AG100_RS04020,EK12AG100_RS04025,EK12AG100_RS04035,EK12AG100_RS04040,EK12AG100_RS04045,bet,EK12AG100_RS04055,EK12AG100_RS25515,EK12AG100_RS25520,EK12AG100_RS25525,EK12AG100_RS04070,EK12AG100_RS04075,EK12AG100_RS04080,EK12AG100_RS04085,EK12AG100_RS04100,EK12AG100_RS04105,EK12AG100_RS04110,EK12AG100_RS04115,EK12AG100_RS04120,EK12AG100_RS25015,EK12AG100_RS04130,EK12AG100_RS04135,EK12AG100_RS04140,EK12AG100_RS04145,EK12AG100_RS23540,EK12AG100_RS04150,EK12AG100_RS04155,EK12AG100_RS04160,EK12AG100_RS04165,EK12AG100_RS04170,EK12AG100_RS04175,EK12AG100_RS23545,EK12AG100_RS04180,EK12AG100_RS04185,rzoD,EK12AG100_RS04195,EK12AG100_RS04200,EK12AG100_RS04205,EK12AG100_RS04210,EK12AG100_RS04215,EK12AG100_RS04220,EK12AG100_RS04225,EK12AG100_RS04230,EK12AG100_RS04235,EK12AG100_RS04240,EK12AG100_RS04245,EK12AG100_RS04250,EK12AG100_RS04255,EK12AG100_RS04260,EK12AG100_RS04265,EK12AG100_RS04270,EK12AG100_RS04275,EK12AG100_RS04280,EK12AG100_RS04285,EK12AG100_RS04290
* * ÷ NZ_LN832404 1438029 1438706 678 47 [45] [46] 47 [ttcA] [ttcA]
* * ÷ NZ_LN832404 1982233 1982527 295 47 [45] [46] 47 flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* * ÷ NZ_LN832404 2561513 2561813 301 47 [46] [46] 47 [EK12AG100_RS12790]–[eutA] [EK12AG100_RS12790],EK12AG100_RS25715,[eutA]
* * ÷ NZ_LN832404 3126623–3127647 3127900 254–1278 47 [46] [0] 381 [EK12AG100_RS15510] [EK12AG100_RS15510]
* * ÷ NZ_LN832404 3420281–3420745 3421000–3420750 6–720 47 [46] [45] 47 [rrf]–[EK12AG100_RS17000] [rrf],[EK12AG100_RS17000]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_LN832404 = 614890 (0.000)10 (0.130) 10/402 NT 100% intergenic (‑76/+87) EK12AG100_RS23030/rapA RNA polymerase‑associated protein RapA/RNA polymerase‑associated protein RapA
?NZ_LN832404 = 381811 NA (NA)coding (847/914 nt) EK12AG100_RS01865 IS3‑like element IS2 family transposase
* ? NZ_LN832404 61489 =0 (0.000)24 (0.320) 22/386 NT 100% intergenic (‑76/+87) EK12AG100_RS23030/rapA RNA polymerase‑associated protein RapA/RNA polymerase‑associated protein RapA
?NZ_LN832404 380645 = NA (NA)coding (41/361 nt) EK12AG100_RS01865 IS3‑like element IS2 family transposase
* ? NZ_LN832404 = 6670016 (0.150)57 (0.580)
+27 bp
52/504 NT 75.5% coding (19/696 nt) araD L‑ribulose‑5‑phosphate 4‑epimerase
?NZ_LN832404 70241 = 25 (0.230)intergenic (‑25/‑314) araB/araC ribulokinase/arabinose operon transcriptional regulator AraC
* ? NZ_LN832404 269933 =NA (NA)95 (0.870) 88/558 NT 56.5% pseudogene (7/72 nt) EK12AG100_RS25460 phage tail protein
?NZ_LN832404 = 371058 73 (0.670)coding (521/867 nt) EK12AG100_RS01800 2‑hydroxy‑6‑oxonona‑2,4‑dienedioate hydrolase
* ? NZ_LN832404 = 274541NA (NA)17 (0.160) 16/558 NT 12.2% intergenic (‑32/‑176) EK12AG100_RS01335/mmuP IS5‑like element IS5 family transposase/S‑methylmethionine permease
?NZ_LN832404 576125 = 122 (1.120)coding (411/411 nt) EK12AG100_RS02865 DUF1398 domain‑containing protein
* ? NZ_LN832404 278555 =108 (0.990)78 (0.720) 4/558 NT 40.4% intergenic (‑2/+15) EK12AG100_RS01355/EK12AG100_RS01360 ABC transporter permease subunit/IS1 family transposase
?NZ_LN832404 = 2756390 122 (1.120)coding (1102/1410 nt) EK12AG100_RS13710 DUF3987 domain‑containing protein
* ? NZ_LN832404 = 36365470 (0.640)47 (0.510)
+42 bp
25/474 NT 40.6% coding (1951/3075 nt) EK12AG100_RS01775 beta‑galactosidase
?NZ_LN832404 3419771 = 92 (0.850)intergenic (+65/‑66) EK12AG100_RS16980/EK12AG100_RS25785 amino acid ABC transporter ATP‑binding protein/paraslipin
* ? NZ_LN832404 371057 =74 (0.680)88 (0.810) 77/558 NT 54.3% coding (520/867 nt) EK12AG100_RS01800 2‑hydroxy‑6‑oxonona‑2,4‑dienedioate hydrolase
?NZ_LN832404 = 1474170 NA (NA)intergenic (+7/‑457) EK12AG100_RS07415/EK12AG100_RS23745 IS30‑like element IS30 family transposase/twice split molybdate metabolism regulator domain protein
* ? NZ_LN832404 = 381810NA (NA)26 (0.330) 24/400 NT 100% coding (846/914 nt) EK12AG100_RS01865 IS3‑like element IS2 family transposase
?NZ_LN832404 = 932184 0 (0.000)intergenic (‑74/‑445) EK12AG100_RS04715/EK12AG100_RS04720 aspartate:alanine antiporter/hypothetical protein
* ? NZ_LN832404 = 578602140 (1.290)9 (0.120) 9/380 NT 15.9% coding (308/744 nt) EK12AG100_RS02890 phage terminase large subunit family protein
?NZ_LN832404 822993 = 0 (0.000)coding (129/1746 nt) EK12AG100_RS04195 phage terminase large subunit family protein
* ? NZ_LN832404 = 80428324 (0.220)96 (0.930) 85/528 NT 85.1% intergenic (‑61/+74) EK12AG100_RS03990/EK12AG100_RS03995 putative acyl‑CoA thioester hydrolase/tyrosine‑type recombinase/integrase
?NZ_LN832404 849715 = 11 (0.110)intergenic (‑766/+90) EK12AG100_RS04330/EK12AG100_RS04335 AAA family ATPase/kinase inhibitor
* ? NZ_LN832404 932185 =0 (0.000)17 (0.220) 16/390 NT 100% intergenic (‑75/‑444) EK12AG100_RS04715/EK12AG100_RS04720 aspartate:alanine antiporter/hypothetical protein
?NZ_LN832404 = 1472810 NA (NA)coding (39/361 nt) EK12AG100_RS07405 IS3‑like element IS2 family transposase
* ? NZ_LN832404 = 1423461NA (NA)23 (0.230)
+27 bp
23/504 NT 100% intergenic (+148/‑125) EK12AG100_RS07185/EK12AG100_RS07190 IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_LN832404 = 2242224 0 (0.000)intergenic (‑1/+2) mglA/mglB galactose/methyl galactoside ABC transporter ATP‑binding protein MglA/galactose/glucose ABC transporter substrate‑binding protein MglB
* ? NZ_LN832404 = 1472808NA (NA)15 (0.200) 13/386 NT 100% coding (41/361 nt) EK12AG100_RS07405 IS3‑like element IS2 family transposase
?NZ_LN832404 = 1870685 0 (0.000)intergenic (‑559/‑65) EK12AG100_RS09380/EK12AG100_RS09385 scaffolding protein MipA/protein kinase YeaG
* ? NZ_LN832404 = 1474132NA (NA)17 (0.180) 16/482 NT 100% coding (1121/1152 nt) EK12AG100_RS07415 IS30‑like element IS30 family transposase
?NZ_LN832404 2694668 = 0 (0.000)intergenic (‑89/+37) yfhb/EK12AG100_RS25720 phosphatidylglycerophosphatase C/phosphatidylglycerophosphatase C
* ? NZ_LN832404 = 1655111NA (NA)14 (0.190) 14/376 NT 100% pseudogene (616/696 nt) EK12AG100_RS08315 IS3‑like element IS2 family transposase
?NZ_LN832404 1870686 = 0 (0.000)intergenic (‑560/‑64) EK12AG100_RS09380/EK12AG100_RS09385 scaffolding protein MipA/protein kinase YeaG
* ? NZ_LN832404 = 2105880NA (NA)17 (0.180)
+32 bp
17/494 NT 100% intergenic (‑33/+2) EK12AG100_RS10660/EK12AG100_RS10665 IS5‑like element IS5 family transposase/glycosyltransferase
?NZ_LN832404 2242225 = 0 (0.000)intergenic (‑2/+1) mglA/mglB galactose/methyl galactoside ABC transporter ATP‑binding protein MglA/galactose/glucose ABC transporter substrate‑binding protein MglB
* ? NZ_LN832404 = 26946690 (0.000)16 (0.220) 15/378 NT 100% intergenic (‑90/+36) yfhb/EK12AG100_RS25720 phosphatidylglycerophosphatase C/phosphatidylglycerophosphatase C
?NZ_LN832404 = 4505063 NA (NA)coding (29/1152 nt) EK12AG100_RS22270 IS30‑like element IS30 family transposase
* ? NZ_LN832404 = 3127562NA (NA)17 (0.220) 17/396 NT 100% intergenic (+65/+418) EK12AG100_RS15510/EK12AG100_RS15515 IS5‑like element IS5 family transposase/lipopolysaccharide biosynthesis protein
?NZ_LN832404 3127901 = 0 (0.000)intergenic (+404/+79) EK12AG100_RS15510/EK12AG100_RS15515 IS5‑like element IS5 family transposase/lipopolysaccharide biosynthesis protein
* ? NZ_LN832404 3127981 =0 (0.000)32 (0.520)
+121 bp
29/316 NT 100% pseudogene (941/942 nt) EK12AG100_RS15515 lipopolysaccharide biosynthesis protein
?NZ_LN832404 3648543 = NA (NA)coding (740/783 nt) EK12AG100_RS18170 hypothetical protein
* ? NZ_LN832404 = 40904639 (0.080)60 (0.620)
+29 bp
57/500 NT 78.4% coding (283/825 nt) rhaD rhamnulose‑1‑phosphate aldolase
?NZ_LN832404 4093925 = 28 (0.260)intergenic (‑4/‑284) rhaB/EK12AG100_RS20290 rhamnulokinase/HTH‑type transcriptional activator RhaS
* ? NZ_LN832404 4539156 =63 (0.580)54 (0.510) 49/540 NT 43.7% intergenic (+49/‑433) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NZ_LN832404 4539470 = 78 (0.740)intergenic (+363/‑119) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NZ_LN832404 = 453916464 (0.590)72 (0.680) 62/540 NT 50.7% intergenic (+57/‑425) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NZ_LN832404 = 4539460 78 (0.740)intergenic (+353/‑129) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA