breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 71,999 T→G 12.0% L217V (TTG→GTG)  yabI → DedA family inner membrane protein
RA 164,581 A→C 19.5% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 165,787 T→G 5.5% M353R (ATG→AGG)  mrcB → fused glycosyl transferase and transpeptidase
RA 222,958 Δ1 bp 100% coding (126/576 nt) gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase
RA 238,523 G→T 6.2% L72I (CTT→ATT)  ykfM ← lethality reduction protein, putative inner membrane protein
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 576,400 T→G 10.6% pseudogene (426/1040 nt) nmpC ← DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
RA 659,204 C→T 5.1% intergenic (+54/+47) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,205 A→T 5.2% intergenic (+55/+46) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,206 A→T 5.5% intergenic (+56/+45) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,207 A→G 5.2% intergenic (+57/+44) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 777,208 A→C 12.1% K289N (AAA→AAC tolA → membrane anchored protein in TolA‑TolQ‑TolR complex
RA 781,238 T→A 5.7% intergenic (+16/‑131) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 843,196 T→G 16.0% F289V (TTT→GTT)  rlmF → 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
RA 1,081,760 T→G 12.1% pseudogene (301/726 nt) efeU → ferrous iron permease (pseudogene)
RA 1,286,823 T→G 5.6% intergenic (+297/+243) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,286,965 A→T 8.7% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,286,983 A→T 7.9% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,351,227 A→C 6.3% intergenic (‑188/+180) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,351,228 G→C 9.7% intergenic (‑189/+179) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,402,466 T→G 5.2% M264L (ATG→CTG)  abgB ← p‑aminobenzoyl‑glutamate hydrolase, B subunit
RA 1,466,201 T→C 5.2% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,495 C→T 5.9% pseudogene (1104/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,562 G→A 5.3% pseudogene (1171/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,630 G→C 6.3% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,639 C→T 6.0% pseudogene (1248/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,630,528 T→G 6.0% Y129S (TAT→TCT)  ydfI ← putative NAD‑dependent D‑mannonate oxidoreductase
RA 1,642,516 T→G 6.4% E242D (GAA→GAC quuQ ← Qin prophage; putative antitermination protein Q
RA 1,662,370 A→C 6.4% K605N (AAA→AAC ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic
RA 1,708,709 T→A 6.2% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,805 T→A 6.9% R629R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,814 A→T 5.1% A632A (GCA→GCT rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,720,364 G→T 6.6% intergenic (+21/+26) slyB → / ← slyA outer membrane lipoprotein/global transcriptional regulator
RA 1,864,768 T→C 6.0% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,964,035 T→G 5.2% K339T (AAA→ACA)  flhA ← putative flagellar export pore protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,177,228 C→A 14.4% intergenic (‑24/+284) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,177,234 C→A 19.7% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,177,245 G→T 16.8% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,229,177 A→C 8.8% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C
RA 2,304,649 C→T 5.3% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,680 T→C 8.2% intergenic (+287/+428) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,688 A→G 9.9% intergenic (+295/+420) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,762 T→C 5.7% intergenic (+369/+346) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,013 A→G 5.4% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,085 A→G 6.1% intergenic (+692/+23) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,086 C→G 6.1% intergenic (+693/+22) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,087 C→T 6.0% intergenic (+694/+21) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
JC JC 2,375,828 IS186 (+) +4 bp 100% coding (132‑135/963 nt) menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,411,234 C→A 5.7% S40I (AGC→ATC)  yfbS ← putative transporter
RA 2,411,241 C→A 7.5% A38S (GCA→TCA)  yfbS ← putative transporter
RA 2,460,555 A→T 5.1% intergenic (‑86/+95) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
RA 2,460,604 A→C 11.5% intergenic (‑135/+46) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
RA 2,470,427 C→A 11.9% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,470,436 C→A 8.6% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,481,750 C→A 9.2% A143S (GCT→TCT)  emrY ← putative multidrug efflux system
RA 2,588,928 T→G 5.6% I445S (ATT→AGT)  acrD → aminoglycoside/multidrug efflux system
RA 2,639,267 T→G 5.4% N6T (AAC→ACC)  yfgM ← ancillary SecYEG translocon subunit; putative anti‑RcsB factor
RA 2,641,051 A→C 5.1% F252V (TTC→GTC)  ispG ← 1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate synthase
RA 2,718,314 A→C 5.3% V72G (GTT→GGT)  yfiF ← putative methyltransferase
RA 2,817,916 T→G 6.0% intergenic (‑56/+143) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,818,046 A→G 6.1% intergenic (‑186/+13) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,818,049 T→A 6.1% intergenic (‑189/+10) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,900,322 C→T 5.7% intergenic (‑49/‑270) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,900,324 G→T 5.5% intergenic (‑51/‑268) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,900,327 A→C 5.6% intergenic (‑54/‑265) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,904,212 G→A 100% intergenic (+838/+535) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 3,046,050 T→G 7.4% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,046,068 T→A 15.6% intergenic (‑167/+100) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,054,888 A→T 8.2% V186E (GTA→GAA)  ygfB ← UPF0149 family protein
RA 3,087,396 T→G 5.9% F231V (TTC→GTC)  metK → S‑adenosylmethionine synthetase
RA 3,239,611 C→A 5.1% intergenic (+66/‑333) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,269,956 T→C 5.3% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,324,952 A→G 5.3% intergenic (‑41/+49) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,955 A→G 5.3% intergenic (‑44/+46) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,959 C→T 5.1% intergenic (‑48/+42) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,351,503 A→C 5.0% V508G (GTA→GGA)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
RA 3,391,407 A→C 17.6% F208V (TTT→GTT)  tldD ← putative peptidase
RA 3,440,923 C→A 100% G36V (GGC→GTC)  rpoA ← RNA polymerase, alpha subunit
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,608,695 C→G 9.8% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,608,721 A→C 8.5% intergenic (+72/+30) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,647,512 T→G 9.4% I405S (ATT→AGT)  gor → glutathione oxidoreductase
RA 3,728,125 T→G 5.7% Y416S (TAC→TCC)  xylB ← xylulokinase
RA 3,781,022 T→G 8.5% intergenic (+5/‑193) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,032 T→G 11.7% intergenic (+15/‑183) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,067 T→G 6.1% intergenic (+50/‑148) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,106 G→C 5.0% intergenic (+89/‑109) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,828,980 A→C 6.1% Q12H (CAA→CAC xanP → xanthine permease
JC JC 3,890,075 IS5 (–) +4 bp 100% coding (1346‑1349/1416 nt) tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,959,457 T→G 6.5% intergenic (+12/+75) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,482 A→C 7.3% intergenic (+37/+50) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,515 T→A 7.4% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 4,070,464 T→G 5.6% intergenic (‑63/+51) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase
RA 4,296,060 C→T 33.2% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,303 A→G 9.0% intergenic (+509/+133) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
RA 4,633,743 C→T 100% M1M (ATG→ATA) † yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase
JC JC 4,640,551 IS1 (–) +9 bp 100% intergenic (+9/‑383) yjjY → / → yjtD uncharacterized protein/putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 772989 774226 1238 308 [297] [295] 306 [cydA]–[cydX] [cydA],cydB,[cydX]
* * ÷ NC_000913 1037673 1039342 1670 304 [300] [299] 304 cbdA–[cbdB] cbdA,[cbdB]
* * ÷ NC_000913 1166008 1167445 1438 304 [297] [303] 308 ndh ndh
* * ÷ NC_000913 3423734–3424234 3424556–3424238 5–823 309 [300] [300] 304 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 274384 =NA (NA)10 (0.020) 4/270 NT NA noncoding (766/1195 nt) IS5 repeat region
?NC_000913 274422 = NA (NA)noncoding (728/1195 nt) IS5 repeat region
* ? NC_000913 274620 =NA (NA)9 (0.020) 5/258 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 781369 =463 (0.760)47 (0.080)
+GAAG
20/268 NT 10.2% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 388 (0.640)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 937227NA (NA)8 (0.010) 5/260 NT NA noncoding (4/36 nt) REP85 (repetitive extragenic palindromic) element; contains 1 REP sequences REP85 (repetitive extragenic palindromic) element; contains 1 REP sequences
?NC_000913 = 937259 NA (NA)noncoding (36/36 nt) REP85 (repetitive extragenic palindromic) element; contains 1 REP sequences REP85 (repetitive extragenic palindromic) element; contains 1 REP sequences
* ? NC_000913 1207790 =291 (0.480)213 (0.400) 108/244 NT 46.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 227 (0.420)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805272 (0.450)240 (0.450) 109/244 NT 50.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 227 (0.420)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)445 (0.760) 169/268 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1843797NA (NA)8 (0.010) 6/264 NT NA noncoding (74/93 nt) REP131 (repetitive extragenic palindromic) element; contains 2 REP sequences REP131 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913 = 1843810 NA (NA)noncoding (87/93 nt) REP131 (repetitive extragenic palindromic) element; contains 2 REP sequences REP131 (repetitive extragenic palindromic) element; contains 2 REP sequences
* ? NC_000913 2727891 =NA (NA)22 (0.040) 4/264 NT NA noncoding (1294/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
?NC_000913 2727910 = NA (NA)noncoding (1275/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
* ? NC_000913 = 2817942421 (0.690)35 (0.070) 20/236 NT 8.4% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?NC_000913 2818492 = 406 (0.780)noncoding (58/77 nt) argV tRNA‑Arg
* ? NC_000913 = 4254000NA (NA)5 (0.010) 5/262 NT NA noncoding (100/130 nt) RIP317 (repetitive extragenic palindromic) element; contains 3 REP sequences and 1 IHF site RIP317 (repetitive extragenic palindromic) element; contains 3 REP sequences and 1 IHF site
?NC_000913 = 4254030 NA (NA)noncoding (130/130 nt) RIP317 (repetitive extragenic palindromic) element; contains 3 REP sequences and 1 IHF site RIP317 (repetitive extragenic palindromic) element; contains 3 REP sequences and 1 IHF site