breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,736 A→T intergenic (+105/‑87) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,903 T→A D27E (GAT→GAA folA → type 3 dihydrofolate reductase
RA 50,280 T→C F153S (TTT→TCT)  folA → type 3 dihydrofolate reductase
RA 56,392 A→C Y240D (TAC→GAC)  lptD ← LPS assembly protein LptD
RA 98,192 T→C I314T (ATT→ACT)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA 108,362 A→G F242S (TTC→TCC)  zapD ← cell division protein ZapD
RA 115,488 T→C F90S (TTC→TCC)  ampD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD
RA 115,628 A→C T137P (ACG→CCG)  ampD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD
RA 139,618 A→G E118G (GAA→GGA)  yadG → ABC transporter ATP‑binding protein
RA 209,716 T→C I300T (ATC→ACC)  tilS → tRNA lysidine(34) synthetase TilS
RA 221,335 T→C noncoding (1078/1542 nt) BW25113_RS01010 → 16S ribosomal RNA
RA 231,042 A→C G76G (GGT→GGG gloB ← hydroxyacylglutathione hydrolase
RA 264,689 (A)5→4 intergenic (‑18/+311) ykfA ← / ← perR GTPase family protein/LysR substrate‑binding domain‑containing protein
RA 297,509 G→C G30G (GGC→GGG paoB ← aldehyde oxidoreductase FAD‑binding subunit PaoB
RA 340,444 (A)5→4 coding (558/816 nt) BW25113_RS01680 → hypothetical protein
RA 354,025 T→C E126G (GAA→GGA)  cynR ← transcriptional regulator CynR
RA 386,045 T→C pseudogene (339/1527 nt) BW25113_RS01915 → outer membrane autotransporter barrel domain‑containing protein
RA 444,519 G→A R527C (CGT→TGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 463,972 A→G D45G (GAC→GGC)  decR → DNA‑binding transcriptional regulator DecR
RA 465,464 T→C S380P (TCG→CCG)  mdlA → SmdA family multidrug ABC transporter permease/ATP‑binding protein
RA 475,878 T→C D96G (GAT→GGT)  tomB ← Hha toxicity modulator TomB
RA 476,525 A→G intergenic (‑361/+185) tomB ← / ← acrB Hha toxicity modulator TomB/efflux RND transporter permease AcrB
RA 502,414 T→C D42G (GAT→GGT)  ybaK ← Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase YbaK
RA 508,601 (C)6→7 coding (570/1293 nt) ybaT → APC family permease
RA 539,223 C→G A94P (GCC→CCC)  allE ← (S)‑ureidoglycine aminohydrolase
RA 539,975 A→G D258D (GAT→GAC allC ← allantoate deiminase
RA 554,427 A→G intergenic (+217/‑3) fimA → / → fimC type 1 fimbrial major subunit FimA/type 1 fimbria chaperone FimC
RA 568,752 A→G G71G (GGA→GGG ybcO → DUF1364 domain‑containing protein
RA 579,943 (T)8→7 intergenic (+57/+193) appY → / ← ompT DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT
RA 592,895 A→C L103F (TTA→TTC cusF → Cu(+)/Ag(+) efflux RND transporter periplasmic metallochaperone CusF
RA 601,986 A→G F285S (TTC→TCC)  ybdK ← YbdK family carboxylate‑amine ligase
RA 609,731 A→G D40G (GAT→GGT)  entF → enterobactin non‑ribosomal peptide synthetase EntF
RA 661,892 A→C G594G (GGT→GGG mrdA ← peptidoglycan DD‑transpeptidase MrdA
RA 674,442 T→G T141P (ACT→CCT)  ybeT ← tetratricopeptide repeat protein
RA 818,875 A→G N107N (AAT→AAC clsB ← cardiolipin synthase ClsB
RA 866,539 C→G A39A (GCC→GCG gsiC → glutathione ABC transporter permease GsiC
RA 874,237 C→T L14F (CTT→TTT)  yliI → aldose sugar dehydrogenase YliI
RA 886,189 A→G G7G (GGT→GGC grxA ← glutaredoxin 1
RA 951,809 T→G T94P (ACC→CCC)  ycaO ← 30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 979,260 T→C I52T (ATC→ACC)  gloC → hydroxyacylglutathione hydrolase GloC
RA 987,365 A→G N430D (AAT→GAT)  pepN → aminopeptidase N
RA 991,969 T→G L144F (TTA→TTC ssuA ← aliphatic sulfonate ABC transporter substrate‑binding protein SsuA
RA 1,021,663 (A)5→4 coding (1737/2055 nt) helD → DNA helicase IV
RA 1,054,234 A→G E232G (GAA→GGA)  torC → pentaheme c‑type cytochrome TorC
RA 1,062,210 T→C G390G (GGT→GGC agp → bifunctional glucose‑1‑phosphatase/inositol phosphatase
RA 1,065,426 T→G T129P (ACC→CCC)  rutF ← pyrimidine utilization flavin reductase protein F
RA 1,076,727 (T)5→4 intergenic (+458/‑85) putP → / → BW25113_RS05295 sodium/proline symporter PutP/FTR1 family protein
RA 1,110,952 T→C intergenic (‑6/+166) mdtG ← / ← lpxL multidrug efflux MFS transporter MdtG/kdo(2)‑lipid IV(A) lauroyltransferase
RA 1,122,270 (A)6→5 coding (9/924 nt) yceM → Gfo/Idh/MocA family oxidoreductase
RA 1,153,703 A→T I127F (ATC→TTC)  ptsG → PTS glucose transporter subunit IIBC
RA 1,174,665 T→G L206W (TTG→TGG)  nagK → N‑acetylglucosamine kinase
RA 1,223,232 (A)8→7 coding (63/294 nt) BW25113_RS06140 → YcgL domain‑containing protein
RA 1,230,259 C→T intergenic (‑86/‑135) nhaB ← / → fadR Na(+)/H(+) antiporter NhaB/fatty acid metabolism transcriptional regulator FadR
RA 1,240,670 Δ1 bp coding (55/441 nt) BW25113_RS06225 → hypothetical protein
RA 1,259,173 A→G T2A (ACC→GCC)  hemA → glutamyl‑tRNA reductase
RA 1,276,251 Δ1 bp coding (932/3744 nt) narG → nitrate reductase subunit alpha
RA 1,320,565 G→A Q521Q (CAG→CAA yciQ → DUF2207 domain‑containing protein
RA 1,325,886 T→C G194G (GGT→GGC topA → type I DNA topoisomerase
RA 1,331,016 A→G Y310C (TAT→TGT)  acnA → aconitate hydratase AcnA
RA 1,336,062 A→G intergenic (+78/‑116) lapB → / → pyrF lipopolysaccharide assembly protein LapB/orotidine‑5'‑phosphate decarboxylase
RA 1,363,696 T→C F16S (TTC→TCC)  pspD → phage shock protein PspD
RA 1,392,826 T→C Q18Q (CAA→CAG uspE ← universal stress protein UspE
RA 1,394,163 G→A L111L (CTG→TTG)  ogt ← methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,394,663 T→C G456G (GGA→GGG abgT ← p‑aminobenzoyl‑glutamate transporter
RA 1,402,000 A→G I18T (ATC→ACC)  dgcM ← diguanylate cyclase DgcM
RA 1,417,788 (C)6→7 coding (69/120 nt) BW25113_RS26130 ← hypothetical protein
RA 1,423,602 T→C R99R (CGT→CGC stfR → prophage tail fiber N‑terminal domain‑containing protein
RA 1,431,536 T→C E1169G (GAA→GGA)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,434,377 A→G F222S (TTC→TCC)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,454,774 A→G D16G (GAT→GGT)  paaI → hydroxyphenylacetyl‑CoA thioesterase PaaI
RA 1,494,123 T→C E195G (GAA→GGA)  ydcK ← YdcK family protein
RA 1,499,566 A→G S258P (TCC→CCC)  ydcO ← BenE family transporter YdcO
RA 1,502,076 A→G N347D (AAC→GAC)  rlhA → 23S rRNA 5‑hydroxycytidine C2501 synthase
RA 1,503,965 T→C D67D (GAT→GAC hicB → type II toxin‑antitoxin system antitoxin HicB
RA 1,505,430 A→G K391E (AAA→GAA)  ydcR → PLP‑dependent aminotransferase family protein
RA 1,524,815 T→C intergenic (‑54/‑28) BW25113_RS25600 ← / → BW25113_RS07655 protein YncO/ISAs1 family transposase
RA 1,531,275 G→A L98L (CTG→TTG)  narW ← nitrate reductase molybdenum cofactor assembly chaperone
RA 1,547,935 A→G V54A (GTA→GCA)  adhP ← alcohol dehydrogenase AdhP
RA 1,558,439 A→G Y518H (TAT→CAT)  dosP ← oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
RA 1,573,157 A→T I148N (ATC→AAC)  yddA ← ABC transporter ATP‑binding protein/permease
RA 1,575,825 A→T D319E (GAT→GAA ydeN ← sulfatase
RA 1,576,388 A→G S132P (TCC→CCC)  ydeN ← sulfatase
RA 1,579,109 A→C G545G (GGT→GGG ydeP ← acid resistance putative oxidoreductase YdeP
RA 1,595,655 A→G intergenic (‑157/‑92) lsrR ← / → lsrA transcriptional regulator LsrR/autoinducer 2 ABC transporter ATP‑binding protein LsrA
RA 1,632,437 (T)7→6 intergenic (‑71/+8) BW25113_RS08135 ← / ← BW25113_RS25620 hypothetical protein/hypothetical protein
RA 1,634,151 Δ1 bp coding (164/534 nt) rrrQ ← lysozyme
RA 1,649,476 A→G intergenic (‑78/+128) rspA ← / ← ynfA starvation‑sensing protein RspA/YnfA family protein
RA 1,659,576 T→C F14S (TTC→TCC)  clcB → voltage‑gated ClC‑type chloride channel ClcB
RA 1,662,649 T→G H58P (CAC→CCC)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,671,899 T→G T88P (ACG→CCG)  pntA ← Re/Si‑specific NAD(P)(+) transhydrogenase subunit alpha
RA 1,673,542 A→G K287E (AAG→GAG)  ydgH → DUF1471 family protein YdgH
RA 1,673,785 T→C intergenic (+157/‑29) ydgH → / → ydgI DUF1471 family protein YdgH/amino acid permease
RA 1,717,498 G→C G374A (GGC→GCC)  ydhK → FUSC family protein
RA 1,803,201 A→G V97A (GTC→GCC)  yniB ← YniB family protein
RA 1,807,028 T→C E121G (GAA→GGA)  BW25113_RS09050 ← hypothetical protein
RA 1,812,807 (C)7→8 intergenic (‑50/+55) celF ← / ← chbR 6‑phospho‑beta‑glucosidase/transcriptional regulator ChbR
RA 1,818,956 T→G K80T (AAA→ACA)  ves ← environmental stress‑induced protein Ves
RA 1,838,813 T→C I106V (ATT→GTT)  ynjI ← DUF1266 domain‑containing protein
RA 1,840,928 T→C E97G (GAA→GGA)  topB ← DNA topoisomerase III
RA 1,844,118 A→G E342G (GAA→GGA)  sppA → signal peptide peptidase SppA
RA 1,848,011 T→C T76A (ACT→GCT)  ydjE ← MFS transporter
RA 1,854,126 T→C M121V (ATG→GTG)  ydjK ← MFS transporter
RA 1,877,816 T→C pseudogene (408/1482 nt) yeaV → BCCT family transporter YeaV
RA 1,897,230 A→G D309G (GAT→GGT)  manX → PTS mannose transporter subunit IIAB
RA 1,900,885 A→G S145P (TCC→CCC)  rlmA ← 23S rRNA (guanine(745)‑N(1))‑methyltransferase
RA 1,903,061 A→T intergenic (‑38/‑121) mgrB ← / → yobH PhoP/PhoQ regulator MgrB/YobH family protein
RA 1,927,010 T→C K2E (AAA→GAA)  kdgA ← bifunctional 4‑hydroxy‑2‑oxoglutarate aldolase/2‑dehydro‑3‑deoxy‑phosphogluconate aldolase
RA 1,934,547 T→C intergenic (‑97/+23) lpxM ← / ← mepM lauroyl‑Kdo(2)‑lipid IV(A) myristoyltransferase/murein DD‑endopeptidase MepM
RA 1,941,604 A→C G10G (GGT→GGG ruvC ← crossover junction endodeoxyribonuclease RuvC
RA 1,965,782 T→G E389D (GAA→GAC tar ← methyl‑accepting chemotaxis protein II
RA 1,966,502 Δ1 bp coding (447/1662 nt) tar ← methyl‑accepting chemotaxis protein II
RA 1,972,691 A→G intergenic (‑237/‑543) flhD ← / → uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
RA 1,980,420 A→G G5G (GGA→GGG ftnB → non‑heme ferritin‑like protein
RA 2,003,480 T→C F60S (TTC→TCC)  yedK → SOS response‑associated peptidase
RA 2,012,918 A→G D351G (GAC→GGC)  fliK → flagellar hook‑length control protein FliK
RA 2,038,183 (T)5→4 intergenic (+78/‑236) BW25113_RS10330 → / → yeeJ tRNA‑Asn/inverse autotransporter adhesin YeeJ
RA 2,057,664 T→C G95G (GGA→GGG cobT ← nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
RA 2,061,342 T→A intergenic (‑576/‑774) BW25113_RS10415 ← / → BW25113_RS24005 IS5‑like element IS5 family transposase/TonB‑dependent receptor plug domain‑containing protein
RA 2,064,259 T→G V93G (GTG→GGG)  BW25113_RS10440 → GTPase family protein
RA 2,102,646 T→C G140G (GGA→GGG wzx ← O16 family O‑antigen flippase
RA 2,122,267 A→C S35A (TCG→GCG)  wcaF ← colanic acid biosynthesis acetyltransferase WcaF
RA 2,140,422 G→C G200G (GGC→GGG alkA ← DNA‑3‑methyladenine glycosylase 2
RA 2,174,381 T→G C270G (TGC→GGC)  yegU → ADP‑ribosylglycohydrolase family protein
RA 2,176,805 T→G D109A (GAC→GCC)  yegX ← glycoside hydrolase family 25 protein
RA 2,189,312 A→G T512A (ACC→GCC)  metG → methionine‑‑tRNA ligase
RA 2,202,171 G→C W242C (TGG→TGC yehP → VWA domain‑containing protein
RA 2,202,478 T→G Y345D (TAT→GAT)  yehP → VWA domain‑containing protein
RA 2,220,044 A→G S236P (TCG→CCG)  yohF ← SDR family oxidoreductase
RA 2,229,956 A→G K405E (AAA→GAA)  preA → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
RA 2,283,874 Δ1 bp intergenic (‑350/‑105) BW25113_RS11435 ← / → narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
RA 2,284,519 T→C S181P (TCC→CCC)  narP → nitrate/nitrite response regulator protein NarP
RA 2,345,414 T→G H147P (CAC→CCC)  glpT ← glycerol‑3‑phosphate transporter
RA 2,358,446 Δ1 bp coding (414/426 nt) nudI → nucleoside triphosphatase NudI
RA 2,396,099 G→C A413G (GCC→GGC)  nuoC ← NADH‑quinone oxidoreductase subunit C/D
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 2,410,868 A→T G103G (GGA→GGT yfcC → putative basic amino acid antiporter YfcC
RA 2,425,778 T→C E215G (GAA→GGA)  folC ← bifunctional tetrahydrofolate synthase/dihydrofolate synthase
RA 2,434,529 A→C F186V (TTT→GTT)  fabB ← beta‑ketoacyl‑ACP synthase I
RA 2,439,101 T→C K255E (AAG→GAG)  mepA ← penicillin‑insensitive murein endopeptidase
RA 2,492,074 (T)5→4 intergenic (‑300/‑76) alaC ← / → ypdA alanine transaminase/two‑component system sensor histidine kinase YpdA
RA 2,505,777 T→C I137V (ATC→GTC)  mntH ← Nramp family divalent metal transporter
RA 2,526,050 A→G T95A (ACC→GCC)  cysK → cysteine synthase A
RA 2,536,106 T→C E261G (GAA→GGA)  cysP ← thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
RA 2,554,121 A→C N169T (AAC→ACC)  BW25113_RS12740 → hypothetical protein
RA 2,661,508 T→G T250P (ACC→CCC)  hcaR ← DNA‑binding transcriptional regulator HcaR
RA 2,670,359 T→C G454G (GGA→GGG yphE ← sugar ABC transporter ATP‑binding protein
RA 2,678,723 A→C G48G (GGT→GGG glyA ← serine hydroxymethyltransferase
RA 2,726,562 (T)5→4 coding (971/2574 nt) clpB ← ATP‑dependent chaperone ClpB
RA 2,739,096 T→C E61G (GAA→GGA)  rimM ← ribosome maturation factor RimM
RA 2,751,237 A→G V241V (GTT→GTC BW25113_RS13685 ← hypothetical protein
RA 2,753,169 T→C S276P (TCA→CCA)  yfjI → YfjI family protein
RA 2,778,934 (T)6→5 intergenic (‑564/+187) BW25113_RS25705 ← / ← BW25113_RS13825 DUF5507 domain‑containing protein/tRNA‑Ile
RA 2,797,377 (T)5→4 coding (517/960 nt) nrdF → class 1b ribonucleoside‑diphosphate reductase subunit beta
RA 2,801,112 A→C T208P (ACC→CCC)  proX → glycine betaine/L‑proline ABC transporter substrate‑binding protein ProX
RA 2,814,267 A→G A368A (GCT→GCC alaS ← alanine‑‑tRNA ligase
RA 2,818,451 T→C E162G (GAA→GGA)  mltB ← lytic murein transglycosylase B
RA 2,819,095 T→C intergenic (‑160/‑96) mltB ← / → srlA lytic murein transglycosylase B/PTS glucitol/sorbitol transporter subunit IIC
RA 2,833,262 T→G V127G (GTC→GGC)  ascF → PTS cellobiose/arbutin/salicin transporter subunit IIBC
RA 2,849,816 T→C E117G (GAA→GGA)  ygbA ← nitrous oxide‑stimulated promoter family protein
RA 2,859,998 T→C I305V (ATT→GTT)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,882,413 A→C V349G (GTT→GGT)  cysI ← assimilatory sulfite reductase (NADPH) hemoprotein subunit
RA 2,982,555 T→C F232S (TTC→TCC)  yqeI → transcriptional regulator
RA 2,995,567 A→G T635A (ACC→GCC)  xdhA → xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,002,903 T→C S261P (TCA→CCA)  ygeY → YgeY family selenium metabolism‑linked hydrolase
RA 3,011,010 A→G E531G (GAA→GGA)  ygfK → putative selenate reductase subunit YgfK
RA 3,098,336 Δ1 bp coding (545/1080 nt) mltC → membrane‑bound lytic murein transglycosylase MltC
RA 3,103,871 (A)7→6 intergenic (+71/+78) BW25113_RS15410 → / ← yghD tRNA‑Phe/GspM family type II secretion system protein YghD
RA 3,113,649 T→G K330N (AAA→AAC glcA ← glycolate permease GlcA
RA 3,179,271 G→A A167T (GCC→ACC)  ygiL → fimbrial‑like protein
RA 3,273,721 A→G E49G (GAA→GGA)  agaV → PTS N‑acetylgalactosamine transporter subunit IIB
RA 3,318,468 A→C A609A (GCT→GCG ftsH ← ATP‑dependent zinc metalloprotease FtsH
RA 3,347,716 A→G intergenic (‑307/‑368) yhcC ← / → gltB TIGR01212 family radical SAM protein/glutamate synthase large subunit
RA 3,458,386 A→G E369G (GAA→GGA)  gspL → type II secretion system protein GspL
RA 3,475,701 T→C S352P (TCG→CCG)  yheS → ABC transporter ATP‑binding protein
RA 3,505,986 T→C intergenic (‑283/+7) BW25113_RS17530 ← / ← trpS GntR family transcriptional regulator YhfZ/tryptophan‑‑tRNA ligase
RA 3,535,339 T→C F525S (TTC→TCC)  feoB → Fe(2+) transporter permease subunit FeoB
RA 3,576,634 A→G R79R (CGA→CGG BW25113_RS17825 → YrhA family protein
RA 3,579,168 T→C E339G (GAA→GGA)  ggt ← gamma‑glutamyltransferase
RA 3,624,386 T→C E522G (GAA→GGA)  yhiJ ← YhiJ/YhiL family protein
RA 3,728,024 A→C S315S (TCA→TCC xylH → xylose ABC transporter permease XylH
RA 3,785,687 G→C G75G (GGC→GGG kbl ← glycine C‑acetyltransferase
RA 3,786,467 T→C I193V (ATT→GTT)  yibB ← protein YibB
RA 3,786,991 T→C D18G (GAT→GGT)  yibB ← protein YibB
RA 3,791,182 A→C Y292S (TAC→TCC)  waaL → O‑antigen ligase
RA 3,798,416 (T)8→7 coding (61/936 nt) rfaS ← LPS core biosynthesis protein RfaS
RA 3,848,051 T→G F257C (TTT→TGT)  emrD → multidrug efflux MFS transporter EmrD
RA 3,852,078 T→G T467P (ACG→CCG)  yidK ← solute:sodium symporter family transporter
RA 3,896,621 A→G D147D (GAT→GAC bglB ← 6‑phospho‑beta‑glucosidase BglB
RA 3,898,828 (T)5→4 coding (130/1878 nt) bglF ← PTS beta‑glucoside transporter subunit IIABC
RA 3,901,121 A→G D202D (GAT→GAC pstB ← phosphate ABC transporter ATP‑binding protein PstB
RA 3,931,448 G→T L265F (TTG→TTT rbsK → ribokinase
RA 3,934,995 +C intergenic (‑308/‑173) yieP ← / → BW25113_RS19520 FadR/GntR family transcriptional regulator/16S ribosomal RNA
RA 4,034,572 T→G Q74H (CAA→CAC mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein MobB
RA 4,064,564 A→G Y185H (TAT→CAT)  yihS ← sulfoquinovose isomerase
RA 4,073,163 A→T D26V (GAT→GTT)  BW25113_RS20185 → hypothetical protein
RA 4,084,653 A→G G358G (GGT→GGC frvB ← PTS fructose‑like transporter subunit IIBC
RA 4,108,830 A→G H143H (CAT→CAC rraA ← ribonuclease E activity regulator RraA
RA 4,117,071 T→C F44S (TTC→TCC)  rpmE → 50S ribosomal protein L31
RA 4,153,816 T→C S84P (TCA→CCA)  btuB → TonB‑dependent vitamin B12 receptor BtuB
RA 4,180,586 (A)7→6 intergenic (+333/+77) yjaZ → / ← thiH heat‑shock protein C/2‑iminoacetate synthase ThiH
RA 4,211,170 (T)8→7 coding (1246/2187 nt) espL4 ← type III secretion system effector EspL4
RA 4,215,724 T→G W657G (TGG→GGG)  metH → methionine synthase
RA 4,306,692 A→T I115N (ATC→AAC)  phnM ← alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase
RA 4,350,381 G→C G457G (GGC→GGG cadC ← lysine decarboxylation/transport transcriptional activator CadC
RA 4,367,563 T→C intergenic (‑24/+65) blc ← / ← ampC lipocalin Blc/BlaEC family class C beta‑lactamase
RA 4,385,675 T→G F92V (TTT→GTT)  tsaE → tRNA (adenosine(37)‑N6)‑threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE
RA 4,409,638 T→C intergenic (‑196/‑159) ulaG ← / → ulaA L‑ascorbate 6‑phosphate lactonase/PTS ascorbate transporter subunit IIC
RA 4,494,539 T→C F222S (TTC→TCC)  nanX → MFS transporter
RA 4,527,151 T→C Q87Q (CAA→CAG nanS ← 9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
RA 4,543,103 A→G E129G (GAA→GGA)  uxuB → fructuronate reductase
RA 4,593,447 T→C S52P (TCC→CCC)  yjjQ → response regulator transcription factor
RA 4,613,222 C→G R238P (CGC→CCC)  lplA ← lipoate‑‑protein ligase LplA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 71434 71457 24 2 [1] [1] 2 thiB thiamine ABC transporter substrate binding subunit
* * ÷ NZ_CP009273 198214 198224 11 4 [1] [1] 2 lpxD UDP‑3‑O‑(3‑hydroxymyristoyl)glucosamine N‑acyltransferase
* * ÷ NZ_CP009273 201444 201468 25 5 [1] [1] 2 rnhB ribonuclease HII
* * ÷ NZ_CP009273 246782 246796 15 2 [1] [1] 2 lafU putative lateral flagellar export/assembly protein LafU
* * ÷ NZ_CP009273 356931 357578 648 2 [1] [1] 2 lacA lacA
* * ÷ NZ_CP009273 428506 428558 53 2 [1] [1] 3 nrdR transcriptional regulator NrdR
* * ÷ NZ_CP009273 463296 463326 31 2 [1] [1] 2 cof HMP‑PP phosphatase
* * ÷ NZ_CP009273 488156 488168 13 2 [0] [1] 2 dnaX DNA polymerase III subunit gamma/tau
* * ÷ NZ_CP009273 525883 525914 32 3 [1] [1] 2 mnmH tRNA 2‑selenouridine(34) synthase MnmH
* * ÷ NZ_CP009273 553091 553106 16 2 [0] [1] 2 folD bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
* * ÷ NZ_CP009273 600896 600980 85 2 [1] [1] 3 [ybdF] [ybdF]
* * ÷ NZ_CP009273 622441 622455 15 3 [1] [1] 2 entE (2,3‑dihydroxybenzoyl)adenylate synthase EntE
* * ÷ NZ_CP009273 650400 650483 84 2 [1] [1] 3 dcuC anaerobic C4‑dicarboxylate transporter DcuC
* * ÷ NZ_CP009273 703506 703577 72 2 [1] [0] 2 glnS/chiP glutamine‑‑tRNA ligase/chitoporin
* * ÷ NZ_CP009273 726354 726381 28 2 [1] [1] 2 rhsC rhs element protein RhsC
* * ÷ NZ_CP009273 748306 748325 20 2 [1] [1] 2 ybgD/gltA fimbrial protein/citrate synthase
* * ÷ NZ_CP009273 748528 748582 55 2 [1] [1] 2 ybgD/gltA fimbrial protein/citrate synthase
* * ÷ NZ_CP009273 772196 772388 193 2 [1] [1] 2 tolA cell envelope integrity protein TolA
* * ÷ NZ_CP009273 787654 787672 19 2 [1] [1] 3 galE/modF UDP‑glucose 4‑epimerase GalE/molybdate ABC transporter ATP‑binding protein ModF
* * ÷ NZ_CP009273 828256 828271 16 2 [0] [1] 2 ybiA N‑glycosidase YbiA
* * ÷ NZ_CP009273 880862 880934 73 2 [1] [1] 2 ybjI 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
* * ÷ NZ_CP009273 902716 902717 2 2 [0] [0] 2 ybjT SDR family oxidoreductase
* * ÷ NZ_CP009273 902983 903094 112 2 [1] [1] 2 ybjT SDR family oxidoreductase
* * ÷ NZ_CP009273 916834 916852 19 2 [1] [1] 3 macB macrolide ABC transporter ATP‑binding protein/permease MacB
* * ÷ NZ_CP009273 961196 961226 31 2 [1] [1] 2 ycaI ComEC family protein
* * ÷ NZ_CP009273 964413 964420 8 3 [1] [1] 2 lpxK tetraacyldisaccharide 4'‑kinase
* * ÷ NZ_CP009273 964858 964873 16 2 [1] [1] 2 ycaQ winged helix DNA‑binding protein YcaQ
* * ÷ NZ_CP009273 988941 988982 42 3 [1] [1] 2 ssuB aliphatic sulfonates ABC transporter ATP‑binding protein
* * ÷ NZ_CP009273 991697 991728 32 3 [1] [1] 2 ssuA aliphatic sulfonate ABC transporter substrate‑binding protein SsuA
* * ÷ NZ_CP009273 1000080 1000086 7 2 [1] [1] 2 ycbF fimbrial chaperone
* * ÷ NZ_CP009273 1023286 1023308 23 3 [1] [0] 3 yccT/yccU DUF2057 family protein/CoA‑binding protein
* * ÷ NZ_CP009273 1061006 1061017 12 2 [1] [0] 4 yccE/agp YccE family protein/bifunctional glucose‑1‑phosphatase/inositol phosphatase
* * ÷ NZ_CP009273 1070173 1070239 67 3 [1] [1] 2 rutR HTH‑type transcriptional regulator RutR
* * ÷ NZ_CP009273 1103862 1103951 90 2 [0] [1] 2 mdoC glucans biosynthesis protein MdoC
* * ÷ NZ_CP009273 1114563 1114595 33 2 [1] [1] 2 yceJ/BW25113_RS05515 cytochrome b/YceO family protein
* * ÷ NZ_CP009273 1137761 1137853 93 2 [0] [1] 2 rne ribonuclease E
* * ÷ NZ_CP009273 1137981 1137994 14 2 [0] [1] 2 rne ribonuclease E
* * ÷ NZ_CP009273 1139321 1139360 40 3 [0] [1] 2 rne ribonuclease E
* * ÷ NZ_CP009273 1151756 1151768 13 2 [1] [1] 2 holB DNA polymerase III subunit delta'
* * ÷ NZ_CP009273 1158176 1158227 52 2 [1] [1] 2 lpoB penicillin‑binding protein activator LpoB
* * ÷ NZ_CP009273 1161553 1161570 18 2 [1] [1] 3 ndh NADH‑quinone dehydrogenase
* * ÷ NZ_CP009273 1167420 1167461 42 2 [1] [1] 2 mfd transcription‑repair coupling factor
* * ÷ NZ_CP009273 1168113 1168156 44 2 [1] [1] 2 mfd transcription‑repair coupling factor
* * ÷ NZ_CP009273 1180374 1180412 39 2 [1] [1] 2 potA spermidine/putrescine ABC transporter ATP‑binding protein PotA
* * ÷ NZ_CP009273 1207571 1207590 20 2 [0] [1] 2 iraM/BW25113_RS25985 anti‑adapter protein IraM/protein YmgK
* * ÷ NZ_CP009273 1211123 1211145 23 3 [0] [1] 2 bluF/ycgZ blue light‑responsive regulator BluF/regulatory protein YcgZ
* * ÷ NZ_CP009273 1215901 1215937 37 2 [1] [1] 2 BW25113_RS25990 autotransporter outer membrane beta‑barrel domain‑containing protein
* * ÷ NZ_CP009273 1223583 1223627 45 2 [1] [1] 3 ycgM fumarylacetoacetate hydrolase family protein
* * ÷ NZ_CP009273 1230543 1230582 40 4 [1] [1] 2 fadR fatty acid metabolism transcriptional regulator FadR
* * ÷ NZ_CP009273 1240560 1240627 68 2 [1] [1] 2 [BW25113_RS06225] [BW25113_RS06225]
* * ÷ NZ_CP009273 1245425 1245453 29 2 [1] [1] 2 dhaK dihydroxyacetone kinase subunit DhaK
* * ÷ NZ_CP009273 1246718 1246730 13 2 [1] [1] 3 dhaR dihydroxyacetone kinase operon transcriptional regulator DhaR
* * ÷ NZ_CP009273 1267965 1268008 44 2 [1] [1] 2 chaC glutathione‑specific gamma‑glutamylcyclotransferase
* * ÷ NZ_CP009273 1273664 1273680 17 2 [1] [1] 3 narK nitrate transporter NarK
* * ÷ NZ_CP009273 1281009 1281040 32 2 [1] [1] 2 narJ nitrate reductase molybdenum cofactor assembly chaperone
* * ÷ NZ_CP009273 1294198 1294218 21 2 [1] [1] 3 ychE NAAT family transporter YchE
* * ÷ NZ_CP009273 1306796 1306808 13 2 [1] [1] 2 yciB septation protein A
* * ÷ NZ_CP009273 1313869 1313869 1 2 [0] [0] 2 trpCF bifunctional indole‑3‑glycerol‑phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF
* * ÷ NZ_CP009273 1318350 1318418 69 2 [1] [1] 3 [yciV]–[yciO] [yciV],[yciO]
* * ÷ NZ_CP009273 1318528 1318554 27 3 [1] [1] 2 yciO L‑threonylcarbamoyladenylate synthase
* * ÷ NZ_CP009273 1320801 1320879 79 2 [1] [1] 2 yciQ DUF2207 domain‑containing protein
* * ÷ NZ_CP009273 1334148 1334218 71 2 [1] [1] 2 pgpB phosphatidylglycerophosphatase B
* * ÷ NZ_CP009273 1350604 1350621 18 2 [1] [0] 2 sapA peptide ABC transporter substrate‑binding protein SapA
* * ÷ NZ_CP009273 1362443 1362534 92 2 [1] [1] 2 pspA phage shock protein PspA
* * ÷ NZ_CP009273 1373225 1373260 36 2 [0] [1] 2 ycjT kojibiose phosphorylase
* * ÷ NZ_CP009273 1393444 1393491 48 2 [1] [0] 3 fnr fumarate/nitrate reduction transcriptional regulator Fnr
* * ÷ NZ_CP009273 1418205 1418214 10 2 [1] [1] 2 trkG Trk system potassium transporter TrkG
* * ÷ NZ_CP009273 1418815 1418824 10 2 [1] [1] 3 trkG Trk system potassium transporter TrkG
* * ÷ NZ_CP009273 1450949 1450962 14 3 [1] [1] 2 paaK phenylacetate‑CoA oxygenase/reductase subunit PaaK
* * ÷ NZ_CP009273 1481000 1481025 26 2 [1] [1] 2 hrpA ATP‑dependent RNA helicase HrpA
* * ÷ NZ_CP009273 1484563 1484568 6 2 [1] [0] 2 gap type I glyceraldehyde‑3‑phosphate dehydrogenase
* * ÷ NZ_CP009273 1509220 1509267 48 3 [1] [1] 2 ydcV ABC transporter permease
* * ÷ NZ_CP009273 1521470 1521475 6 2 [0] [1] 2 yncH/BW25113_RS07645 YncH family protein/RHS repeat protein
* * ÷ NZ_CP009273 1684717 1684747 31 2 [1] [1] 2 ydgA YdgA family protein
* * ÷ NZ_CP009273 1686600 1686610 11 5 [0] [1] 2 uidC glucuronide uptake porin UidC
* * ÷ NZ_CP009273 1687108 1687158 51 2 [1] [1] 2 [uidC]–[uidB] [uidC],[uidB]
* * ÷ NZ_CP009273 1789497 1789526 30 2 [1] [1] 3 [ihfA] [ihfA]
* * ÷ NZ_CP009273 1807553 1807583 31 2 [0] [1] 2 BW25113_RS09050/cedA hypothetical protein/cell division activator CedA
* * ÷ NZ_CP009273 1818576 1818610 35 3 [1] [0] 2 cho excinuclease Cho
* * ÷ NZ_CP009273 1929080 1929100 21 3 [1] [1] 2 [zwf] [zwf]
* * ÷ NZ_CP009273 1967650 1967659 10 2 [0] [1] 3 cheA chemotaxis protein CheA
* * ÷ NZ_CP009273 2010188 2010271 84 2 [1] [1] 2 fliI flagellar protein export ATPase FliI
* * ÷ NZ_CP009273 2042763 2042774 12 2 [1] [0] 3 yeeJ inverse autotransporter adhesin YeeJ
* * ÷ NZ_CP009273 2099582 2099619 38 2 [0] [1] 2 wzy O16 family O‑antigen polymerase
* * ÷ NZ_CP009273 2140745 2140845 101 2 [1] [1] 2 alkA DNA‑3‑methyladenine glycosylase 2
* * ÷ NZ_CP009273 2149054 2149110 57 2 [1] [1] 2 mdtB multidrug efflux RND transporter permease subunit MdtB
* * ÷ NZ_CP009273 2151941 2151944 4 2 [1] [0] 2 mdtC multidrug efflux RND transporter permease subunit MdtC
* * ÷ NZ_CP009273 2212393 2212414 22 3 [1] [0] 2 osmF glycine betaine ABC transporter substrate‑binding protein OsmF
* * ÷ NZ_CP009273 2224885 2224938 54 2 [1] [1] 3 yohK CidB/LrgB family autolysis modulator
* * ÷ NZ_CP009273 2466865 2466937 73 2 [1] [1] 3 BW25113_RS26000 helix‑turn‑helix domain‑containing protein
* * ÷ NZ_CP009273 2563823 2563854 32 2 [1] [1] 2 eutE aldehyde dehydrogenase EutE
* * ÷ NZ_CP009273 2823971 2824035 65 2 [1] [1] 2 gutQ arabinose‑5‑phosphate isomerase GutQ
* * ÷ NZ_CP009273 3178644 3178673 30 2 [0] [1] 2 ubiK/ygiL ubiquinone biosynthesis accessory factor UbiK/fimbrial‑like protein
* * ÷ NZ_CP009273 3492602 3492628 27 3 [1] [1] 2 cysG/yhfL siroheme synthase CysG/YhfL family protein
* * ÷ NZ_CP009273 3623885 3623933 49 3 [0] [1] 2 yhiI HlyD family efflux transporter periplasmic adaptor subunit
* * ÷ NZ_CP009273 3661067 3661074 8 2 [0] [1] 2 gadA/ccp glutamate decarboxylase/cytochrome c peroxidase
* * ÷ NZ_CP009273 4221720 4221730 11 2 [1] [0] 3 yjbD/lysC DUF3811 domain‑containing protein/lysine‑sensitive aspartokinase 3
* * ÷ NZ_CP009273 4272450 4272458 9 2 [0] [1] 3 espX5 T3SS effector pentapeptide repeat protein EspX5
* * ÷ NZ_CP009273 4294358 4294387 30 2 [1] [1] 2 ytcA/yjcS YtcA family lipoprotein/alkyl sulfatase YjcS
* * ÷ NZ_CP009273 4303906 4303934 29 2 [1] [1] 2 yjdP protein YjdP
* * ÷ NZ_CP009273 4587913 4587934 22 2 [1] [1] 3 opgB phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)23 (1.790) 15/170 0.1 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 19727511 (0.080)22 (1.710) 16/170 0.1 95.7% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP