breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 18,243 A→G 10.5% E252G (GAG→GGG)  nhaA → sodium‑proton antiporter
RA 37,255 A→C 18.1% G190G (GGT→GGG caiC ← putative crotonobetaine/carnitine‑CoA ligase
RA 52,342 A→C 9.5% V30G (GTG→GGG)  rsmA ← 16S rRNA m(6)A1518, m(6)A1519 dimethyltransferase, SAM‑dependent
RA 57,545 T→G 12.1% V61G (GTG→GGG)  djlA → membrane‑anchored DnaK co‑chaperone, DNA‑binding protein
RA 173,223 A→G 9.3% S588G (AGT→GGT)  fhuB → iron(3+)‑hydroxamate import ABC transporter permease
RA 199,049 T→G 12.4% G374G (GGT→GGG bamA → BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
RA 230,100 A→C 8.8% V261G (GTG→GGG)  yafC ← LysR family putative transcriptional regulator
RA 243,044 T→C 9.2% E87G (GAG→GGG)  fadE ← acyl coenzyme A dehydrogenase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 300,989 A→G 9.5% L300P (CTC→CCC)  paoB ← PaoABC aldehyde oxidoreductase, FAD‑containing subunit
RA 334,255 T→C 9.9% E60G (GAG→GGG)  yahB ← putative DNA‑bindng transcriptional regulator
RA 335,249 G→A 8.1% intergenic (‑227/‑31) yahC ← / → yahD putative inner membrane protein/ankyrin repeat protein
RA 380,139 A→C 11.7% V235G (GTG→GGG)  yaiO ← outer membrane protein
RA 386,014 T→G 11.1% G261G (GGT→GGG tauA → taurine ABC transporter periplasmic binding protein
RA 388,319 C→G 17.4% R175R (CGC→CGG tauD → taurine dioxygenase, 2‑oxoglutarate‑dependent
RA 388,772 T→C 11.7% E319G (GAG→GGG)  hemB ← 5‑aminolevulinate dehydratase (porphobilinogen synthase)
RA 429,830 T→G 14.1% V109G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit
RA 433,649 T→G 35.8% G65G (GGT→GGG ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
JC JC 458,790 IS186 (–) +6 bp :: Δ1 bp 62.8% intergenic (+90/‑93) clpX → / → lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
RA 462,674 A→G 9.6% S254G (AGC→GGC)  ppiD → periplasmic folding chaperone, has an inactive PPIase domain
RA 511,938 T→C 10.1% S100P (TCA→CCA)  glsA → glutaminase 1
RA 540,546 C→T 10.1% intergenic (+38/‑19) allB → / → ybbY allantoinase/putative uracil/xanthine transporter
RA 540,600 A→T 11.0% L12F (TTA→TTT ybbY → putative uracil/xanthine transporter
RA 540,610 C→T 9.7% Q16* (CAG→TAG)  ybbY → putative uracil/xanthine transporter
RA 540,632 G→T 13.2% C23F (TGC→TTC)  ybbY → putative uracil/xanthine transporter
RA 693,429 T→A 10.7% intergenic (‑51/+102) ybeZ ← / ← miaB heat shock protein, putative NTPase; PhoH‑like protein/tRNA‑i(6)A37 methylthiotransferase
RA 706,050 Δ1 bp 6.0% intergenic (+160/‑43) nagE → / → glnS N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components/glutamyl‑tRNA synthetase
RA 740,177 A→G 9.9% E224G (GAG→GGG)  phr → deoxyribodipyrimidine photolyase, FAD‑binding
RA 753,618 T→G 10.3% H284P (CAC→CCC)  gltA ← citrate synthase
RA 758,596 G→C 17.0% intergenic (+191/‑110) sdhB → / → sucA succinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 806,020 A→C 8.5% V421G (GTG→GGG)  ybhC ← acyl‑CoA thioesterase, lipoprotein
RA 816,752 T→A 11.6% intergenic (‑105/‑292) ybhK ← / → moaA putative CofD superfamily transferase/molybdopterin biosynthesis protein A
RA 845,227 A→C 19.3% V85G (GTG→GGG)  ybiO ← mechanosensitive channel protein, intermediate conductance
RA 861,215 G→C 12.9% R464R (CGC→CGG ybiW ← putative pyruvate formate lyase
RA 910,397 T→C 13.6% E218G (GAG→GGG)  poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
RA 910,811 T→G 22.8% H80P (CAC→CCC)  poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
RA 921,692 T→G 13.7% V449G (GTG→GGG)  macB → macrolide ABC transporter peremase/ATPase
RA 954,388 C→T 23.8% A27T (GCA→ACA)  focA ← formate channel
RA 964,532 A→G 5.9% G71G (GGA→GGG ycaI → ComEC family inner membrane protein
RA 973,698 A→G 13.4% G54G (GGA→GGG smtA → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
RA 974,068 C→A 11.1% R178R (CGG→AGG)  smtA → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
RA 1,030,269 C→T 17.6% S69F (TCT→TTT)  yccX → weak acylphosphatase
RA 1,058,486 T→C 16.8% S135P (TCG→CCG)  torC → trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
RA 1,078,659 T→G 17.8% H75P (CAC→CCC)  putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
RA 1,136,671 T→G 12.7% V133G (GTG→GGG)  flgI → putative flagellar basal body protein
RA 1,196,305 C→T 63.9% R395C (CGT→TGT)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,213,890 A→T 12.2% I57K (ATA→AAA)  bluR ← repressor of blue light‑responsive genes
RA 1,239,855 A→G 17.6% E326G (GAG→GGG)  dadX → alanine racemase, catabolic, PLP‑binding
RA 1,241,337 A→C 19.5% G245G (GGT→GGG cvrA ← putative cation/proton antiporter
RA 1,251,973 T→C 15.9% L303P (CTC→CCC)  dhaR → dhaKLM operon transcription activator
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,365,542 T→G 9.7% intergenic (+30/‑8) puuB → / → puuE gamma‑glutamylputrescine oxidoreductase/4‑aminobutyrate aminotransferase, PLP‑dependent
RA 1,366,268 T→G 13.2% V240G (GTG→GGG)  puuE → 4‑aminobutyrate aminotransferase, PLP‑dependent
RA 1,445,981 T→G 22.0% G34G (GGT→GGG ydbL → DUF1318 family protein
RA 1,455,846 A→C 14.5% H228P (CAC→CCC)  paaC → ring 1,2‑phenylacetyl‑CoA epoxidase subunit
RA 1,467,068 T→G 16.1% pseudogene (1677/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,531,410 C→A 7.7% pseudogene (86/315 nt) yncI → pseudogene
RA 1,537,275 T→C 12.1% E12G (GAG→GGG)  narW ← nitrate reductase 2 (NRZ), delta subunit (assembly subunit)
RA 1,543,530 A→C 15.0% S177R (AGT→AGG narU ← nitrate/nitrite transporter
RA 1,614,336 T→C 14.7% E123G (GAG→GGG)  sad ← succinate semialdehyde dehydrogenase, NAD(P)+‑dependent
RA 1,668,252 T→C 10.3% S105G (AGC→GGC)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor
RA 1,706,790 C→A 9.9% T148K (ACG→AAG)  rsxB → SoxR iron‑sulfur cluster reduction factor component; putative iron‑sulfur protein
RA 1,710,100 A→T 8.4% Y320F (TAT→TTT)  rsxD → SoxR iron‑sulfur cluster reduction factor component; putative membrane protein of electron transport complex
RA 1,810,544 A→G 16.2% S112G (AGC→GGC)  ydjM → inner membrane protein regulated by LexA
RA 1,823,068 A→C 12.2% H204P (CAC→CCC)  nadE → NAD synthetase, NH3/glutamine‑dependent
RA 1,825,130 G→T 20.8% intergenic (‑193/+10) ves ← / ← spy cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
RA 1,842,798 T→G 17.0% V143G (GTG→GGG)  gdhA → glutamate dehydrogenase, NADP‑specific
RA 1,861,244 A→C 10.7% V30G (GTG→GGG)  ydjL ← putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 1,879,473 A→C 21.0% V38G (GTG→GGG)  yoaG ← uncharacterized protein
RA 1,923,379 C→T 16.7% E110K (GAA→AAA)  yebY ← DUF2511 family protein
RA 1,955,112 G→T 10.9% G632G (GGC→GGA torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit
RA 1,961,224 T→G 11.1% V388G (GTG→GGG)  argS → arginyl‑tRNA synthetase
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,041,503 T→G 12.1% G43G (GGT→GGG zinT → zinc and cadmium binding protein, periplasmic
RA 2,097,266 C→A 12.3% intergenic (+41/+55) hisI → / ← wzzB phosphoribosyl‑AMP cyclohydrolase and phosphoribosyl‑ATP pyrophosphatase/regulator of length of O‑antigen component of lipopolysaccharide chains
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,193,510 A→C 11.1% V220G (GTG→GGG)  mrp ← antiporter inner membrane protein
RA 2,195,709 C→G 6.7% R470R (CGC→CGG metG → methionyl‑tRNA synthetase
RA 2,253,949 A→C 10.2% V66G (GTG→GGG)  nupX ← nucleoside permease
RA 2,274,349 T→G 21.8% G57G (GGT→GGG yejB → microcin C ABC transporter permease
RA 2,282,167 A→C 13.6% T512T (ACA→ACC yejH → putative ATP‑dependent DNA or RNA helicase
RA 2,285,462 T→C 9.3% S363P (TCG→CCG)  yejM → essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor
RA 2,303,243 A→G 14.3% S85S (TCT→TCC napF ← ferredoxin‑type protein, role in electron transfer to periplasmic nitrate reductase NapA
RA 2,321,376 T→C 7.4% L445P (CTC→CCC)  atoS → sensory histidine kinase in two‑component regulatory system with AtoC
RA 2,398,640 A→C 11.3% V509G (GTG→GGG)  nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,503,025 C→A 9.8% L487F (TTG→TTT fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,527,397 T→G 16.1% V151G (GTG→GGG)  yfeH → putative inorganic ion transporter
JC JC 2,536,312 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp 26.0% coding (479‑484/510 nt) crr → glucose‑specific enzyme IIA component of PTS
RA 2,608,253 C→G 26.2% A446G (GCG→GGG)  hyfF → hydrogenase 4, membrane subunit
RA 2,659,475 T→C 10.3% intergenic (‑8/+88) hscB ← / ← iscA HscA co‑chaperone, J domain‑containing protein Hsc56; IscU‑specific chaperone HscAB/FeS cluster assembly protein
RA 2,714,233 T→C 10.3% intergenic (+3/+206) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,777,459 A→C 11.3% T3P (ACT→CCT)  ypjF → CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system
RA 2,778,040 T→C 12.5% intergenic (+258/+106) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,793,675 T→G 15.2% V314G (GTG→GGG)  gabT → 4‑aminobutyrate aminotransferase, PLP‑dependent
RA 2,794,877 A→G 9.5% I209V (ATC→GTC)  gabP → gamma‑aminobutyrate transporter
RA 2,815,007 T→C 13.4% E478G (GAG→GGG)  gshA ← glutamate‑cysteine ligase
RA 2,820,043 T→C 15.8% S657G (AGC→GGC)  alaS ← alanyl‑tRNA synthetase
RA 2,828,506 T→G 22.1% V257G (GTG→GGG)  srlD → sorbitol‑6‑phosphate dehydrogenase
RA 2,853,016 A→C 10.2% H294P (CAC→CCC)  hypD → hydrogenase maturation protein
RA 2,880,919 G→A 6.1% L44F (CTT→TTT)  casD ← CRISP RNA (crRNA) containing Cascade antiviral complex protein
RA 2,936,843 T→C 9.5% S420S (TCT→TCC fucI → L‑fucose isomerase
RA 2,975,093 T→G 18.4% T308P (ACT→CCT)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
RA 2,978,166 T→A 26.2% Y245F (TAT→TTT)  lysA ← diaminopimelate decarboxylase, PLP‑binding
RA 2,978,476 G→C 11.9% R142G (CGC→GGC)  lysA ← diaminopimelate decarboxylase, PLP‑binding
RA 2,999,610 A→T 14.1% R94R (CGT→CGA ygeR ← LysM domain‑containing M23 family putative peptidase; septation lipoprotein
RA 3,003,095 A→G 7.9% E161G (GAG→GGG)  xdhB → xanthine dehydrogenase, FAD‑binding subunit
RA 3,027,731 A→G 14.6% E204G (GAG→GGG)  ghxQ → guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
RA 3,135,441 T→G 11.5% T311P (ACC→CCC)  pitB ← phosphate transporter
RA 3,141,102 T→C 11.6% D62G (GAT→GGT)  hybD ← maturation protease for hydrogenase 2
RA 3,141,137 G→C 14.3% G50G (GGC→GGG hybD ← maturation protease for hydrogenase 2
RA 3,160,891 T→C 10.8% S86G (AGC→GGC)  ygiQ ← Radical SAM superfamily protein
RA 3,162,372 A→G 9.1% S100P (TCA→CCA)  ftsP ← septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)
RA 3,184,576 T→C 15.3% intergenic (‑110/‑264) ribB ← / → yqiC 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/BMFP family putative fusogenic protein
RA 3,209,974 A→C 12.9% G190G (GGT→GGG tsaD ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; glycation binding protein
RA 3,220,858 T→G 14.7% S455R (AGT→AGG patA → putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
RA 3,277,426 G→A 11.3% E30E (GAG→GAA yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
RA 3,300,864 T→C 13.6% E138G (GAG→GGG)  yhbT ← SCP‑2 sterol transfer family protein
RA 3,315,740 A→C 7.9% V92G (GTG→GGG)  infB ← translation initiation factor IF‑2
RA 3,338,869 T→C 12.9% E127G (GAG→GGG)  mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
RA 3,373,350 A→G 11.7% L76P (CTC→CCC)  nanA ← N‑acetylneuraminate lyase
RA 3,437,054 A→C 11.6% P179P (CCA→CCC trkA → NAD‑binding component of TrK potassium transporter
RA 3,487,702 A→C 13.4% A304A (GCA→GCC yhfK → putative transporter, FUSC superfamily inner membrane protein
RA 3,499,609 A→C 22.9% A54A (GCA→GCC yhfL → small lipoprotein
RA 3,532,535 G→A 8.4% intergenic (‑96/‑283) yhgE ← / → pck DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase [ATP]
RA 3,532,555 G→A 9.7% intergenic (‑116/‑263) yhgE ← / → pck DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase [ATP]
RA 3,534,880 A→C 10.4% V330G (GTG→GGG)  envZ ← sensory histidine kinase in two‑component regulatory system with OmpR
RA 3,551,917 A→C 18.3% V186G (GTG→GGG)  malP ← maltodextrin phosphorylase
RA 3,553,285 T→G 15.3% G67G (GGT→GGG malT → mal regulon transcriptional activator
RA 3,560,455:1 +G 91.9% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,561,266 G→C 8.9% R63G (CGT→GGT)  glpG ← rhomboid intramembrane serine protease
RA 3,563,346 A→G 13.3% E445G (GAG→GGG)  glpD → sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
RA 3,697,740 T→G 15.7% V428G (GTG→GGG)  bcsE → cellulose production protein
RA 3,741,500 T→C 6.6% E28G (GAG→GGG)  ysaA ← putative hydrogenase, 4Fe‑4S ferredoxin‑type component
RA 3,784,900 A→C 13.2% V39G (GTG→GGG)  yibN ← putative rhodanese‑related sulfurtransferase
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,820,320 G→C 13.3% A284G (GCG→GGG)  ligB ← DNA ligase, NAD(+)‑dependent
RA 3,820,372 A→C 8.4% W267G (TGG→GGG)  ligB ← DNA ligase, NAD(+)‑dependent
RA 3,833,715 T→G 12.9% T275P (ACT→CCT)  yicI ← putative alpha‑glucosidase
RA 3,885,168 T→C 8.6% P31P (CCT→CCC yidC → membrane protein insertase
RA 3,932,230 C→T 12.5% I305I (ATC→ATT kup → potassium transporter
RA 3,932,686 T→C 10.6% S457S (TCT→TCC kup → potassium transporter
RA 3,949,065 T→C 13.4% E302G (GAG→GGG)  yifB ← magnesium chelatase family protein and putative transcriptional regulator
RA 3,952,893 A→G 11.5% E137G (GAG→GGG)  ilvE → branched‑chain amino acid aminotransferase
MC JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,011,490 A→G 9.2% T139A (ACT→GCT)  yigM → putative inner membrane EamA‑like transporter
RA 4,011,698 T→G 12.1% V208G (GTG→GGG)  yigM → putative inner membrane EamA‑like transporter
RA 4,022,323 A→G 12.5% I36V (ATT→GTT)  tatB → TatABCE protein translocation system subunit
RA 4,022,863 A→C 7.4% H43P (CAC→CCC)  tatC → TatABCE protein translocation system subunit
RA 4,065,580 T→A 14.1% T63S (ACC→TCC)  yihO ← putative sulphoquinovose importer
RA 4,073,924 T→G 12.8% G62G (GGT→GGG yihV → 6‑deoxy‑6‑sulphofructose kinase
RA 4,101,255 C→T 9.8% S149F (TCT→TTT)  sodA → superoxide dismutase, Mn
RA 4,103,607 T→C 15.4% S457G (AGT→GGT)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR
RA 4,140,198 T→G 12.2% H675P (CAC→CCC)  frwA ← putative PTS enzyme: Hpr, enzyme I and II components
RA 4,158,653 T→G 15.2% V55G (GTG→GGG)  oxyR → oxidative and nitrosative stress transcriptional regulator
RA 4,159,201 A→C 11.4% T238P (ACT→CCT)  oxyR → oxidative and nitrosative stress transcriptional regulator
RA 4,160,854 C→T 44.0% intergenic (‑64/‑270) sthA ← / → fabR pyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,195,535 T→C 9.1% S189G (AGC→GGC)  thiC ← phosphomethylpyrimidine synthase
RA 4,246,567 A→G 10.7% intergenic (‑148/‑217) malE ← / → malK maltose transporter subunit/maltose ABC transportor ATPase
RA 4,277,560 A→T 14.1% Y31F (TAT→TTT)  soxR → redox‑sensitive transcriptional activator of soxS; autorepressor
RA 4,295,700 T→G 26.9% V407G (GTG→GGG)  gltP → glutamate/aspartate:proton symporter
RA 4,296,060 C→T 20.2% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,319,404 A→C 19.4% V68G (GTG→GGG)  phnJ ← carbon‑phosphorus lyase, SAM‑dependent
RA 4,353,194 T→G 11.7% intergenic (+113/+6) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
RA 4,382,744 A→G 13.3% G34G (GGA→GGG epmA → Elongation Factor P Lys34 lysyltransferase
RA 4,437,248 A→G 13.1% E165G (GAG→GGG)  cysQ → 3'(2'),5'‑bisphosphate nucleotidase
RA 4,440,952 G→A 10.5% F88F (TTC→TTT ytfL ← UPF0053 family inner membrane protein
RA 4,443,934 T→G 11.2% V518G (GTG→GGG)  tamA → translocation and assembly module for autotransporter export, outer membrane subunit
RA 4,444,674 T→G 13.3% V188G (GTG→GGG)  tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,449,045 T→G 20.4% intergenic (+3/+77) chpB → / ← ppa toxin of the ChpB‑ChpS toxin‑antitoxin system/inorganic pyrophosphatase
RA 4,452,852 A→G 30.3% G94G (GGA→GGG ytfT → putative sugar ABC transporter permease
RA 4,455,978 T→G 10.6% V65G (GTG→GGG)  mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
RA 4,458,424 A→C 19.7% T156P (ACC→CCC)  pmbA → putative antibiotic peptide MccB17 maturation peptidase
RA 4,481,799 T→C 14.1% E680G (GAG→GGG)  valS ← valyl‑tRNA synthetase
RA 4,485,026 T→C 12.8% E309G (GAG→GGG)  pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
RA 4,522,897 A→G 11.3% S400P (TCC→CCC)  yjhG ← putative dehydratase
RA 4,542,117 A→G 10.8% R27R (AGA→AGG fimE → tyrosine recombinase/inversion of on/off regulator of fimA
RA 4,542,272 A→G 8.8% E79G (GAG→GGG)  fimE → tyrosine recombinase/inversion of on/off regulator of fimA
RA 4,549,430 G→T 10.7% C208F (TGT→TTT)  fimH → minor component of type 1 fimbriae
RA 4,576,032 T→C 7.0% pseudogene (2114/2937 nt) yjiV → pseudogene; conserved hypothetical protein
RA 4,606,902 A→G 20.2% S267P (TCC→CCC)  rsmC ← 16S rRNA m(2)G1207 methyltransferase, SAM‑dependent
RA 4,633,067 T→G 11.0% V103G (GTG→GGG)  trpR → transcriptional repressor, tryptophan‑binding
RA 4,638,873 A→T 10.3% L232F (TTA→TTT creD → inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 954468 954800 333 32 [31] [27] 32 focA/ycaO formate channel/ribosomal protein S12 methylthiotransferase accessory factor
* * ÷ NC_000913 2470080 2470506 427 34 [28] [30] 32 [gtrS] [gtrS]
* * ÷ NC_000913 4233739 4235491 1753 33 [31] [31] 33 pgi pgi

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =22 (0.210)90 (0.920) 59/394 NT 83.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 16 (0.160)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780523 (0.220)106 (1.080) 60/394 NT 85.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 16 (0.160)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)22 (0.210) 20/418 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785020 (0.000)45 (0.440) 37/410 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 4110716NA (NA)3 (0.030) 3/408 NT NA noncoding (7/19 nt) REP303 (repetitive extragenic palindromic) element; contains 1 REP sequences REP303 (repetitive extragenic palindromic) element; contains 1 REP sequences
?NC_000913 = 4110721 NA (NA)noncoding (12/19 nt) REP303 (repetitive extragenic palindromic) element; contains 1 REP sequences REP303 (repetitive extragenic palindromic) element; contains 1 REP sequences