breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA AM260479 160,041 G→C G102A (GGG→GCG)  h16_A0146 → conserved hypothetical protein
RA AM260479 160,043 G→C V103L (GTA→CTA)  h16_A0146 → conserved hypothetical protein
RA AM260479 208,932 A→C H322Q (CAT→CAG h16_A0195 ← Putative GTPase (G3E family)
RA AM260479 208,953 A→C V315V (GTT→GTG h16_A0195 ← Putative GTPase (G3E family)
RA AM260479 245,288 T→G L247V (TTG→GTG)  betA2 → choline dehydrogenase
RA AM260479 275,969 +C coding (268/558 nt) h16_A0264 ← Hypothetical protein
RA AM260479 342,407 T→C L180L (TTG→CTG)  amtB → ammonium transporter
RA AM260479 406,488 G→T L70I (CTC→ATC)  h16_A0388 ← ABC‑type transporter, ATPase component: HAAT family
RA AM260479 452,997 Δ1 bp intergenic (‑22/+116) h16_A0432 ← / ← coxF Uncharacterized MobA‑related protein/xanthine and CO dehydrogenase, maturation factor
RA AM260479 500,512 G→A W23* (TGG→TAG)  h16_A0473 → ABC‑type transporter, permease component: PAAT family
RA AM260479 518,612 G→C intergenic (+17/‑48) h16_A0492 → / → pgam2 Phospholipid‑binding protein/phosphoglycerate mutase 2 protein
RA AM260479 547,818 +C intergenic (‑10/+27) glyS ← / ← h16_A0522 Glycyl‑tRNA synthetase beta subunit/conserved hypothetical protein
RA AM260479 547,839 Δ1 bp intergenic (‑31/+6) glyS ← / ← h16_A0522 Glycyl‑tRNA synthetase beta subunit/conserved hypothetical protein
RA AM260479 547,879 2 bp→GA coding (595‑596/630 nt) h16_A0522 ← conserved hypothetical protein
RA AM260479 561,006 +G intergenic (+9/+38) h16_A0532 → / ← h16_A0533 conserved hypothetical protein/universal stress protein, UspA family
RA AM260479 564,366 C→G P73A (CCC→GCC)  h16_A0537 → Uncharacterized protein conserved in bacteria
RA AM260479 581,821 +C coding (117/1734 nt) h16_A0555 → zinc metalloprotease
RA AM260479 582,589 T→G H295Q (CAT→CAG h16_A0555 → zinc metalloprotease
RA AM260479 582,759 G→T C352F (TGT→TTT)  h16_A0555 → zinc metalloprotease
RA AM260479 619,859 G→T H108N (CAC→AAC)  acrA1 ← Cation/multidrug efflux system, mebrane‑fusion component
RA AM260479 628,642 Δ1 bp coding (756/1206 nt) h16_A0595 ← Uncharacterized Fe‑S protein
RA AM260479 628,654 +C coding (744/1206 nt) h16_A0595 ← Uncharacterized Fe‑S protein
RA AM260479 637,111 +G intergenic (‑164/+218) adk ← / ← kdsB adenylate kinase/3‑Deoxy‑manno‑octulosonate cytidylyltransferase
RA AM260479 724,768 +C coding (82/669 nt) lysE → lysine efflux permease
RA AM260479 735,524 +G intergenic (‑409/+175) h16_A0685 ← / ← h16_A0686 hypothetical membrane spanning protein/Predictedpermease/CBS‑domain‑containing membrane protein
RA AM260479 782,330 +G intergenic (‑680/‑42) h16_A0723 ← / → h16_A0724 hypothetical membrane protein/hypothetical membrane protein
RA AM260479 782,333 +A intergenic (‑683/‑39) h16_A0723 ← / → h16_A0724 hypothetical membrane protein/hypothetical membrane protein
RA AM260479 787,769 +T intergenic (‑549/+743) h16_A0728 ← / ← h16_A0729 response regulator containing CheY‑like receiver, AAA‑type ATPase, and DNA‑binding domains/transcriptional regulator, LysR‑family
RA AM260479 787,882 +T intergenic (‑662/+630) h16_A0728 ← / ← h16_A0729 response regulator containing CheY‑like receiver, AAA‑type ATPase, and DNA‑binding domains/transcriptional regulator, LysR‑family
RA AM260479 916,512 T→C V341A (GTG→GCG)  h16_A0836 → predicted exporter of the RND superfamily
MC JC AM260479 973,838 Δ1,320 bp [h16_A0881]h16_A0882 [h16_A0881], h16_A0882
RA AM260479 1,042,407 T→G K131N (AAA→AAC h16_A0947 ← Coproporphyrinogen III oxidase, Fe‑S oxidoreductase
RA AM260479 1,056,947 +G intergenic (+211/‑15) h16_A0964 → / → h16_A0965 conserved hypothetical protein/conserved hypothetical protein
RA AM260479 1,071,935 +G coding (626/627 nt) h16_A0979 → Hypothetical protein
RA AM260479 1,071,996 +G intergenic (+60/‑123) h16_A0979 → / → h16_A0980 Hypothetical protein/Hypothetical protein
RA AM260479 1,076,499 C→T P267L (CCG→CTG)  cpaF3 → flp pilus assembly ATPase CpaF
RA AM260479 1,076,517 C→T P273L (CCG→CTG)  cpaF3 → flp pilus assembly ATPase CpaF
RA AM260479 1,081,436 +C coding (437/447 nt) h16_A0989 → Hypothetical protein
RA AM260479 1,081,530 T→C L30L (TTG→CTG)  h16_A0990 → outer membrane protein and related peptidoglycan‑associated (lipo)proteins
RA AM260479 1,089,440 Δ1 bp intergenic (+115/‑17) h16_A0996 → / → h16_A0997 FAD/FMN‑containing dehydrogenase/conserved hypothetical protein
RA AM260479 1,112,158 Δ1 bp coding (2295/2469 nt) xdhB1 ← xanthine dehydrogenase, subunit B
RA AM260479 1,167,791 T→C intergenic (+238/‑365) h16_A1070 → / → cyoA1 acyl dehydratase/bo3‑type quinol oxidase, subunit II
RA AM260479 1,191,426 Δ1 bp coding (350/390 nt) h16_A1095 ← Hypothetical protein
RA AM260479 1,191,771 Δ1 bp coding (5/390 nt) h16_A1095 ← Hypothetical protein
RA AM260479 1,231,117 Δ1 bp coding (2606/2652 nt) glnE → glutamine synthetase adenylyltransferase
RA AM260479 1,231,142 Δ1 bp coding (2631/2652 nt) glnE → glutamine synthetase adenylyltransferase
RA AM260479 1,231,143 C→G P878A (CCC→GCC)  glnE → glutamine synthetase adenylyltransferase
RA AM260479 1,231,177 Δ1 bp intergenic (+14/‑201) glnE → / → h16_A1128 glutamine synthetase adenylyltransferase/Membrane protein
RA AM260479 1,231,226 2 bp→CA intergenic (+63/‑151) glnE → / → h16_A1128 glutamine synthetase adenylyltransferase/Membrane protein
RA AM260479 1,240,057 +T intergenic (‑57/‑218) h16_A1136 ← / → grpE conserved hypothetical protein/molecular chaperone GrpE
RA AM260479 1,354,645 +C intergenic (+98/‑15) h16_A1248 → / → h16_A1249 predicted signal transduction protein containingCHASE, PAS, GGDEF and EAL domains/Hypothetical protein
RA AM260479 1,402,540 C→G G893A (GGC→GCC)  h16_A1292 ← ATP‑dependent transcriptional regulator,MalT‑family
RA AM260479 1,464,365 Δ1 bp coding (49/552 nt) h16_A1354 → conserved hypothetical protein
RA AM260479 1,464,433 A→G Q39Q (CAA→CAG h16_A1354 → conserved hypothetical protein
RA AM260479 1,464,435 T→C L40P (CTG→CCG)  h16_A1354 → conserved hypothetical protein
RA AM260479 1,464,441 T→C V42A (GTG→GCG)  h16_A1354 → conserved hypothetical protein
RA AM260479 1,492,923 T→C L191L (TTG→CTG)  pdhB → dihydrolipoamide acetyltransferase (E2) component of pyruvate dehydrogenase complex
RA AM260479 1,492,953 T→G S201A (TCA→GCA)  pdhB → dihydrolipoamide acetyltransferase (E2) component of pyruvate dehydrogenase complex
RA AM260479 1,493,307 T→G F319V (TTC→GTC)  pdhB → dihydrolipoamide acetyltransferase (E2) component of pyruvate dehydrogenase complex
RA AM260479 1,744,688 G→C G211A (GGC→GCC)  h16_A1608 → conserved hypothetical protein containing von Willebrand factor (vWF) type A
RA AM260479 1,841,200 +G intergenic (‑2736/‑2680) h16_A1684 ← / → ycaC conserved hypothetical protein/YcaC related amidohydrolase
RA AM260479 2,016,501 T→C V678V (GTT→GTC h16_A1857 → Uncharacterized protein involved in exopolysaccharide biosynthesis
RA AM260479 2,111,013 +G coding (553/1089 nt) h16_A1942 → Hypothetical protein
RA AM260479 2,111,023 +G coding (563/1089 nt) h16_A1942 → Hypothetical protein
RA AM260479 2,253,193 +C coding (243/258 nt) h16_A2071 → Hypothetical protein
RA AM260479 2,274,430 C→G G565G (GGG→GGC h16_A2092 ← Lysophospholipase, contains weak SAM‑dependent methyltransferase domain
RA AM260479 2,303,559 A→C S217R (AGT→AGG h16_A2116 ← Putative DNA polymerase bacteriophage‑type,uracil‑DNA glycosylase superfamily
RA AM260479 2,517,378 T→G P77A (CCA→GCC zntA ← putative heavy metal transporting P‑type ATPase
RA AM260479 2,517,380 G→C P77A (CCA→GCC)  zntA ← putative heavy metal transporting P‑type ATPase
RA AM260479 2,517,386 A→G Y75H (TAT→CAT)  zntA ← putative heavy metal transporting P‑type ATPase
RA AM260479 2,579,212 G→C A121A (GCC→GCG h16_A2374 ← Metallopeptidase, M23B subfamily
RA AM260479 2,579,243 A→G I111T (ATT→ACT)  h16_A2374 ← Metallopeptidase, M23B subfamily
RA AM260479 2,580,039 A→G R155R (CGT→CGC h16_A2375 ← Protein‑L‑isoaspartate carboxylmethyltransferase
RA AM260479 2,594,082 T→C Q124Q (CAA→CAG cR ← Recombinational DNA repair protein (RecF pathway)
RA AM260479 2,594,128 Δ1 bp coding (326/957 nt) cR ← Recombinational DNA repair protein (RecF pathway)
RA AM260479 2,616,505 C→G A45G (GCC→GGC)  h16_A2404 → predicted esterase of the alpha‑beta hydrolase superfamily
RA AM260479 2,787,923 +C intergenic (+165/‑55) cafA2 → / → moaA1 Ribonuclease G and E/molybdopterin biosynthesis protein
RA AM260479 2,907,305 2 bp→AT coding (181‑182/1221 nt) h16_A2693 → conserved hypothetical protein
RA AM260479 2,907,313 Δ1 bp coding (189/1221 nt) h16_A2693 → conserved hypothetical protein
RA AM260479 2,907,329 Δ1 bp coding (205/1221 nt) h16_A2693 → conserved hypothetical protein
RA AM260479 2,907,333 Δ1 bp coding (209/1221 nt) h16_A2693 → conserved hypothetical protein
RA AM260479 2,955,197 Δ1 bp coding (1034/1071 nt) h16_A2735 → phenylpropionate dioxygenase or related ring‑hydroxylating dioxygenase, large terminal subunit
RA AM260479 3,247,959 2 bp→CA coding (723‑724/813 nt) h16_A3001 → transcriptional regulator, LysR‑family
RA AM260479 3,247,987 Δ1 bp coding (751/813 nt) h16_A3001 → transcriptional regulator, LysR‑family
RA AM260479 3,247,992 Δ1 bp coding (756/813 nt) h16_A3001 → transcriptional regulator, LysR‑family
RA AM260479 3,247,999 Δ1 bp coding (763/813 nt) h16_A3001 → transcriptional regulator, LysR‑family
RA AM260479 3,248,007 Δ1 bp coding (771/813 nt) h16_A3001 → transcriptional regulator, LysR‑family
RA AM260479 3,248,010 C→G A258A (GCC→GCG h16_A3001 → transcriptional regulator, LysR‑family
RA AM260479 3,248,045 Δ1 bp coding (809/813 nt) h16_A3001 → transcriptional regulator, LysR‑family
RA AM260479 3,248,064 +A intergenic (+15/‑203) h16_A3001 → / → hemK1 transcriptional regulator, LysR‑family/methylase of polypeptide chain release factors
RA AM260479 3,248,092 G→C intergenic (+43/‑175) h16_A3001 → / → hemK1 transcriptional regulator, LysR‑family/methylase of polypeptide chain release factors
RA AM260479 3,285,635 G→C G181R (GGC→CGC)  nadB → L‑Aspartate oxidase
RA AM260479 3,301,576 +G intergenic (+45/‑146) lspA → / → dfp lipoprotein signal peptidase II/phosphopantothenoylcysteine synthetase/decarboxylase
RA AM260479 3,302,920 +C coding (1199/1251 nt) dfp → phosphopantothenoylcysteine synthetase/decarboxylase
RA AM260479 3,333,763 +G coding (5/615 nt) serB2 → phosphoserine phosphatase
MC JC AM260479 3,399,144 Δ1,321 bp [leuS]h16_A3140 [leuS], h16_A3140
RA AM260479 3,461,712 +G coding (823/969 nt) h16_A3204 ← transcriptional regulator, LysR‑family
RA AM260479 3,515,018 C→G noncoding (32/75 nt) tRNA‑Pro (CGG) → tRNA‑Pro
RA AM260479 3,515,314 Δ1 bp intergenic (+253/‑570) tRNA‑Pro (CGG) → / → pilB tRNA‑Pro/Type IV pilus assembly protein PilB
RA AM260479 3,515,428 +C intergenic (+367/‑456) tRNA‑Pro (CGG) → / → pilB tRNA‑Pro/Type IV pilus assembly protein PilB
RA AM260479 3,529,526 +G intergenic (‑40/‑264) secA ← / → h16_A3265 preprotein translocase subunit SecA/Hypothetical protein
RA AM260479 3,529,607 +C intergenic (‑121/‑183) secA ← / → h16_A3265 preprotein translocase subunit SecA/Hypothetical protein
RA AM260479 3,602,642 +A intergenic (‑39/+87) h16_A3328 ← / ← h16_A3329 conserved hypothetical membrane protein/unassigned peptidase, M61 subfamily (glycyl aminopeptidase)
RA AM260479 3,602,688 (G)6→5 intergenic (‑85/+41) h16_A3328 ← / ← h16_A3329 conserved hypothetical membrane protein/unassigned peptidase, M61 subfamily (glycyl aminopeptidase)
RA AM260479 3,615,716 +C intergenic (+42/‑147) prfA → / → h16_A3341 protein chain release factor A (RF‑1)/Hypothetical protein
RA AM260479 3,615,722 +A intergenic (+48/‑141) prfA → / → h16_A3341 protein chain release factor A (RF‑1)/Hypothetical protein
RA AM260479 3,720,402 G→T D170Y (GAC→TAC)  h16_A3441 → periplasmic multimodular transpeptidase/transglycosylase
RA AM260479 3,905,524 G→C A1285G (GCC→GGC)  putA ← bifunctional proline dehydrogenase and proline oxidase
RA AM260479 3,972,184 +C intergenic (‑136/‑395) h16_A3682 ← / → h16_A3683 putative methyltransferase/Hypothetical protein
RA AM260479 3,983,831 C→T D23N (GAC→AAC)  ntrX ← response regulator containing a Fis‑type DNA‑binding domain, NtrX
RA AM260479 3,998,635 +C intergenic (‑221/+527) h16_A3705 ← / ← h16_A3706 transcriptional regulator, LysR‑family/conserved hypothetical protein
RA AM260480 69,908 +G intergenic (+89/‑184) h16_B0060 → / → h16_B0061 conserved hypothetical membrane protein/ABC‑type transporter, periplasmic component
RA AM260480 153,689 +G intergenic (‑67/+93) h16_B0133 ← / ← h16_B0134 conserved hypothetical protein/outer membrane protein (porin)
RA AM260480 157,096 +G intergenic (‑26/+41) h16_B0136 ← / ← h16_B0137 cysteine desulfurase/conserved hypothetical membrane protein
RA AM260480 159,381 +G intergenic (‑13/‑342) h16_B0138 ← / → h16_B0139 transcriptional regulator, IclR‑family/conserved hypothetical transmembrane protein
RA AM260480 159,389 +G intergenic (‑21/‑334) h16_B0138 ← / → h16_B0139 transcriptional regulator, IclR‑family/conserved hypothetical transmembrane protein
RA AM260480 164,760 C→T A15V (GCC→GTC)  acoR → transcriptional regulator of acetoin catabolism,PspF‑family
RA AM260480 274,470 (G)10→9 intergenic (+18/‑626) h16_B0246 → / → fhaB Hypothetical protein/filamentous hemagglutinin / adhesin
RA AM260480 293,407 T→A Y759N (TAC→AAC)  flhF → Flagellar GTP‑binding protein
RA AM260480 433,189 A→C R133R (CGA→CGC h16_B0381 → Acetyl‑CoA acetyltransferase
RA AM260480 507,425 +A coding (27/564 nt) h16_B0448 → fragment of ABC‑type transport system,periplasmic component: APC family
RA AM260480 635,877 T→C E259E (GAA→GAG fliM ← flagellar motor switch protein
RA AM260480 753,133 T→C K37E (AAG→GAG)  h16_B0675 ← Acyl‑CoA dehydrogenase,short‑chain specific
RA AM260480 919,006 T→G C159G (TGC→GGC)  h16_B0812 → hydrolase or acyltransferase (alpha/beta hydrolase superfamily)
RA AM260480 951,073 +G intergenic (‑29/+230) h16_B0838 ← / ← h16_B0839 conserved hypothetical protein/transcriptional regulator, CRP‑family
RA AM260480 963,917 Δ1 bp intergenic (+84/‑3) h16_B0849 → / → h16_B0850 Acyl‑CoA dehydrogenase/Acyl‑CoA dehydrogenase, short‑chain specific
RA AM260480 978,518 A→G S117G (AGC→GGC)  h16_B0862 → cytosine deaminase or related metal‑dependent hydrolase
RA AM260480 1,166,676 +G intergenic (+150/‑109) h16_B1032 → / → h16_B1033 conserved hypothetical protein/probable extra‑cytoplasmic solute receptor
RA AM260480 1,192,562 +C coding (163/594 nt) cccA → cytochrome c, mono‑or diheme variant
RA AM260480 1,326,746 +G intergenic (+47/‑123) h16_B1172 → / → h16_B1173 Amidase/MFS transporter
RA AM260480 1,326,751 +G intergenic (+52/‑118) h16_B1172 → / → h16_B1173 Amidase/MFS transporter
RA AM260480 1,326,761 +G intergenic (+62/‑108) h16_B1172 → / → h16_B1173 Amidase/MFS transporter
RA AM260480 2,018,102 +C intergenic (+111/+98) h16_B1778 → / ← h16_B1779 putative peptidase/putative peptidase, S33 family
RA AM260480 2,135,859 +C intergenic (‑237/‑97) h16_B1889 ← / → h16_B1890 transcriptional regulator, IclR‑family/APC transporter, AAT family
RA AM260480 2,295,879 T→A Q603L (CAG→CTG)  h16_B2042 ← Hypothetical protein
RA AM260480 2,308,233 (C)5→4 intergenic (+176/‑161) h16_B2050 → / → h16_B2051 response regulator containing a GGDEF effector domain/conserved hypothetical protein
RA AM260480 2,431,093 Δ1 bp coding (2380/2469 nt) h16_B2148 → membrane carboxypeptidase
RA AM260480 2,737,499 A→G intergenic (‑216/+28) h16_B2423 ← / ← h16_B2424 periplasmic solute binding protein/Hypothetical protein
RA AM260480 2,737,505 Δ1 bp intergenic (‑222/+22) h16_B2423 ← / ← h16_B2424 periplasmic solute binding protein/Hypothetical protein
RA AM260480 2,737,510 T→G intergenic (‑227/+17) h16_B2423 ← / ← h16_B2424 periplasmic solute binding protein/Hypothetical protein
RA AM260480 2,843,580 +G intergenic (‑1/+68) pip ← / ← h16_B2516 prolyl aminopeptidase/transcriptional regulator, LysR‑family
MC JC AM260480 2,912,452 Δ39 bp intergenic (+243/–) h16_B2579 → /  cold shock protein, DNA‑binding/–
RA AY305378 682 C→A P190T (CCG→ACG)  hoxK → membrane‑bound [NiFe] hydrogenase small subunit
JC JC AY305378 84,810 ISAE1 (+) +8 bp [hoxW] [hoxW]

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ AM260479 24065 24083 19 3 [1] [0] 2 h16_A0015 conserved hypothetical protein
* * ÷ AM260479 55287 55326 40 5 [1] [0] 2 h16_A0040 predicted membrane protein
* * ÷ AM260479 156371 156440 70 2 [0] [1] 5 [h16_A0142] [h16_A0142]
* * ÷ AM260479 381495 381639 145 2 [1] [0] 2 ftsY signal recognition particle receptor FtsY
* * ÷ AM260479 442932 443001 70 2 [1] [0] 2 h16_A0423 multidrug resistance efflux pump
* * ÷ AM260479 522675 522685 11 2 [0] [0] 2 ruvB holliday junction resolvasome, helicase subunit RuvB
* * ÷ AM260479 570430 570516 87 2 [1] [1] 2 h16_A0542/h16_A0543 signal transduction histidine kinase/response regulator, OmpR‑family
* * ÷ AM260479 602913 602962 50 3 [1] [1] 5 [sua] [sua]
* * ÷ AM260479 625248 625286 39 2 [1] [1] 3 [h16_A0591] [h16_A0591]
* * ÷ AM260479 663334 663376 43 4 [0] [0] 2 h16_A0625 outer membrane protein (porin)
* * ÷ AM260479 718129 718167 39 2 [0] [0] 4 h16_A0667 Sodium:sulfate symporter transmembrane region
* * ÷ AM260479 791374 791394 21 5 [1] [0] 2 [h16_A0732] [h16_A0732]
* * ÷ AM260479 791431 791528 98 2 [0] [1] 4 h16_A0732 Beta‑propeller domains of methanol dehydrogenase‑type (homolog)
* * ÷ AM260479 805394 805414 21 2 [0] [0] 3 h16_A0741 conserved hypothetical protein
* * ÷ AM260479 922796 922819 24 2 [0] [0] 2 paaJ 3‑Ketoacyl‑CoA‑thiolase P‑44/SCP2
* * ÷ AM260479 1017300 1017334 35 2 [0] [0] 2 h16_A0921 Aerobic‑type carbon monoxide dehydrogenase homolog, small subunit
* * ÷ AM260479 1109813 1109875 63 3 [1] [0] 2 [add] [add]
* * ÷ AM260479 1129299 1129380 82 2 [1] [0] 2 [h16_A1031] [h16_A1031]
* * ÷ AM260479 1228098 1228119 22 2 [0] [0] 3 [h16_A1126] [h16_A1126]
* * ÷ AM260479 1381183 1381246 64 3 [1] [1] 2 h16_A1272 conserved hypothetical protein
* * ÷ AM260479 1639401 1639403 3 2 [0] [0] 4 gatA3/h16_A1510 Glutamyl/Aspartyl‑tRNA(Gln/Asp) amidotransferasesubunit A/predicted transcription regulator, contains HTH domain (MarR family)
* * ÷ AM260479 1646997 1647056 60 5 [1] [0] 2 [h16_A1515]–[ispZ] [h16_A1515],[ispZ]
* * ÷ AM260479 1723450 1723519 70 3 [1] [1] 2 [h16_A1589] [h16_A1589]
* * ÷ AM260479 1741042 1741064 23 2 [0] [0] 2 h16_A1604 MoxR‑like ATPase
* * ÷ AM260479 2154180 2154194 15 2 [0] [0] 2 h16_A1978/h16_A1979 conserved hypothetical protein/conserved hypothetical protein
* * ÷ AM260479 2158457 2158464 8 2 [0] [0] 2 h16_A1984 Hypothetical protein
* * ÷ AM260479 2208795 2208832 38 2 [0] [1] 2 h16_A2032 hypothetical membrane associated protein
* * ÷ AM260479 2253282 2253359 78 3 [1] [1] 4 h16_A2072 predicted permease
* * ÷ AM260479 2426137 2426150 14 2 [0] [1] 2 [lytT] [lytT]
* * ÷ AM260479 2449856 2449876 21 2 [0] [1] 2 h16_A2254 ABC‑type transporter, permease component
* * ÷ AM260479 2463471 2463536 66 6 [1] [1] 2 h16_A2268/h16_A2269 uncharacterized conserved protein/bacterioferritin comigratory protein
* * ÷ AM260479 2546034 2546153 120 2 [0] [1] 4 argAB acetylglutamate kinase and acetyltransferase
* * ÷ AM260479 2651960 2652002 43 6 [0] [0] 6 ppk1 polyphosphate kinase
* * ÷ AM260479 2685658 2685658 1 2 [0] [0] 2 gloB2 hydroxyacylglutathione hydrolase
* * ÷ AM260479 2723559 2723624 66 2 [0] [1] 2 h16_A2508 Glycerol‑3‑phosphate dehydrogenase
* * ÷ AM260479 3026541 3026587 47 2 [1] [1] 2 [asmA] [asmA]
* * ÷ AM260479 3032753 3032777 25 2 [1] [1] 3 h16_A2804 conserved hypothetical protein
* * ÷ AM260479 3041920 3041920 1 2 [0] [0] 2 h16_A2814 predicted metal‑dependent hydrolase/Putative cyclase
* * ÷ AM260479 3341373 3341417 45 3 [1] [0] 2 [h16_A3087] [h16_A3087]
* * ÷ AM260479 3360881 3360917 37 2 [0] [0] 2 h16_A3107 4‑Hydroxybenzoate octaprenyltransferase
* * ÷ AM260479 3365812 3365835 24 2 [1] [0] 2 cG ATP‑dependent DNA helicase
* * ÷ AM260479 3452013 3452031 19 2 [1] [0] 2 [h16_A3193] [h16_A3193]
* * ÷ AM260479 3462503 3462557 55 3 [1] [0] 2 [h16_A3204] [h16_A3204]
* * ÷ AM260479 3472992 3473005 14 2 [0] [0] 2 h16_A3218/h16_A3219 transcriptional regulator, TetR/AcrR‑family/Hypothetical protein
* * ÷ AM260479 3482494 3482519 26 2 [0] [0] 4 h16_A3225/h16_A3226 ABC‑type transporter, ATPase and permease components/Hypothetical protein
* * ÷ AM260479 3548181 3548215 35 3 [0] [0] 2 [ftsI1]–[ftsL] [ftsI1],[ftsL]
* * ÷ AM260479 3554446 3554532 87 2 [1] [1] 6 h16_A3287 Hypothetical protein
* * ÷ AM260479 3609115 3609186 72 3 [1] [1] 3 [h16_A3334] [h16_A3334]
* * ÷ AM260479 3671716 3671761 46 2 [0] [1] 2 h16_A3390/h16_A3391 signal transduction histidine kinase/Hypothetical protein related to helicases
* * ÷ AM260479 3722896 3722979 84 4 [1] [0] 2 h16_A3442/lysA2 protein implicated in iron transport, frataxin homolog/diaminopimelate decarboxylase
* * ÷ AM260479 3857089 3857160 72 3 [1] [1] 2 h16_A3590 C4‑dicarboxylate transport protein
* * ÷ AM260479 3871540 3871608 69 2 [0] [1] 2 [h16_A3603] [h16_A3603]
* * ÷ AM260479 3910001 3910038 38 2 [0] [0] 2 priA Primosomal protein N'
* * ÷ AM260479 4004528 4004553 26 2 [0] [1] 3 h16_A3710 response regulator, NarL‑family
* * ÷ AM260480 81706 81725 20 6 [1] [1] 4 fmdA1/h16_B0073 Formamidase/ABC‑type transporter
* * ÷ AM260480 174665–175127 179995–175130 4–5331 3 [2] [1] 4 rrsD–[rrfD] rrsD,tRNA‑Ile (GAT),tRNA‑Ala (TGC),rrlD,[rrfD]
* * ÷ AM260480 193566 193615 50 4 [2] [1] 5 h16_B0168 putative multidrug resistance protein
* * ÷ AM260480 237928 237954 27 4 [2] [1] 3 [h16_B0211] [h16_B0211]
* * ÷ AM260480 334552 334605 54 4 [2] [2] 4 h16_B0298/h16_B0299 transcriptional regulator, LysR‑family/putative glucose/sorbosone dehydrogenase
* * ÷ AM260480 496899 496920 22 4 [0] [2] 3 [nhaA] [nhaA]
* * ÷ AM260480 545502 545541 40 3 [2] [2] 3 [h16_B0481]–[h16_B0482] [h16_B0481],[h16_B0482]
* * ÷ AM260480 571266 571296 31 6 [0] [0] 8 h16_B0507 Hypothetical protein
* * ÷ AM260480 665349 665360 12 4 [2] [1] 4 h16_B0592 conserved hypothetical protein
* * ÷ AM260480 751998 752045 48 3 [1] [2] 3 h16_B0674/h16_B0675 conserved hypothetical protein/Acyl‑CoA dehydrogenase,short‑chain specific
* * ÷ AM260480 757301 757350 50 3 [1] [1] 3 [h16_B0678] [h16_B0678]
* * ÷ AM260480 867731–872735 872735 1–5005 4 [2] [2] 4 [rrsE]–[rrfE] [rrsE],tRNA‑Ile (GAT),tRNA‑Ala (TGC),rrlE,[rrfE]
* * ÷ AM260480 1152682 1152731 50 3 [2] [2] 3 h16_B1021 Glycine‑rich protein
* * ÷ AM260480 1201096 1201165 70 8 [2] [2] 4 dnaE2 DNA polymerase III alpha subunit
* * ÷ AM260480 1208494 1208527 34 3 [0] [2] 4 h16_B1068 conserved hypothetical protein
* * ÷ AM260480 1222279 1222299 21 5 [0] [1] 23 h16_B1080/tyrB2 Hypothetical protein/tyrosine aminotransferase
* * ÷ AM260480 1319239 1319264 26 3 [1] [2] 3 h16_B1167/h16_B1168 Hypothetical protein/conserved hypothetical protein
* * ÷ AM260480 1434783 1434858 76 3 [1] [2] 4 h16_B1277 hypothetical membrane associated protein
* * ÷ AM260480 1465490 1465533 44 3 [2] [2] 3 h16_B1311 Hypothetical protein
* * ÷ AM260480 1482266 1482295 30 3 [2] [1] 5 h16_B1335 Acyl‑CoA synthetase (AMP‑forming)/AMP‑acid ligase II
* * ÷ AM260480 1632945 1633020 76 3 [2] [2] 4 [h16_B1458] [h16_B1458]
* * ÷ AM260480 1861305 1861354 50 4 [2] [2] 3 h16_B1648 Hypothetical protein
* * ÷ AM260480 2226939 2226959 21 3 [2] [1] 6 h16_B1971 Sensor protein rcsC
* * ÷ AM260480 2459863 2459903 41 4 [2] [2] 3 h16_B2171 Glycosyltransferase, probably involved in cell wall biogenesis
* * ÷ AM260480 2476820 2476867 48 3 [2] [2] 3 copS signal transduction histidine kinase, copper sensor
* * ÷ AM260480 2532171 2532212 42 4 [2] [1] 3 bkdA1 2‑Oxoisovalerate dehydrogenase E1 component,alpha subunit
* * ÷ AM260480 2608826 2608872 47 5 [2] [2] 4 [h16_B2307] [h16_B2307]
* * ÷ AM260480 2647116 2647170 55 5 [2] [2] 3 h16_B2337 conserved hypothetical protein
* * ÷ AM260480 2866443 2866463 21 4 [2] [0] 3 h16_B2539 conserved hypothetical protein
* * ÷ AY305378 85326 85346 21 2 [0] [1] 4 [hoxI] [hoxI]
* * ÷ AY305378 100104 100226 123 3 [1] [1] 3 kfrA1 putative KfrA protein
* * ÷ AY305378 145727 145748 22 2 [0] [0] 2 PHG148/PHG149 conserved hypothetical protein/hypothetical protein
* * ÷ AY305378 151827 151896 70 2 [0] [0] 3 [PHG153] [PHG153]
* * ÷ AY305378 155981 156001 21 2 [1] [0] 2 PHG154/PHG155 hypothetical protein/hypothetical protein
* * ÷ AY305378 156558 156626 69 2 [0] [0] 2 kfrA2 putative KfrA‑like protein
* * ÷ AY305378 156828 156838 11 3 [1] [0] 2 PHG157 conserved hypothetical protein
* * ÷ AY305378 235078 235093 16 3 [1] [0] 9 ccmF cytochrome C‑type biogenesis protein
* * ÷ AY305378 451823 451867 45 2 [0] [0] 2 cbbLp/– ribulose‑1,5‑bisphosphate carboxylase/oxygenase large subunit/–

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AM260479 = 43310716 (0.600)4 (0.160) 4/198 2.0 21.7% coding (285/624 nt) pilV3 Type IV pilus assembly protein PilV
?AM260479 433120 = 14 (0.570)coding (298/624 nt) pilV3 Type IV pilus assembly protein PilV
* ? AM260479 553951 =12 (0.450)4 (0.160) 4/206 2.0 27.1% coding (444/999 nt) phoH phosphate starvation‑inducible protein PhoH,predicted ATPase
?AM260479 553972 = 10 (0.390)coding (423/999 nt) phoH phosphate starvation‑inducible protein PhoH,predicted ATPase
* ? AM260479 1230588 =14 (0.530)5 (0.200) 5/198 1.7 29.4% coding (2077/2652 nt) glnE glutamine synthetase adenylyltransferase
?AM260479 1230615 = 11 (0.450)coding (2104/2652 nt) glnE glutamine synthetase adenylyltransferase
* ? AM260479 3395698 =24 (0.900)7 (0.310) 6/182 1.4 24.8% coding (138/1059 nt) holA DNA polymerase III delta subunit
?AM260479 3395714 = 22 (0.970)coding (122/1059 nt) holA DNA polymerase III delta subunit
* ? AM260479 = 361201615 (0.560)4 (0.160) 4/198 2.0 23.6% coding (133/828 nt) pfs 5‑Methylthioadenosine nucleosidase
?AM260479 = 3612064 12 (0.490)coding (181/828 nt) pfs 5‑Methylthioadenosine nucleosidase
* ? AM260479 = 387515511 (0.410)3 (0.120) 3/194 2.2 24.0% coding (2277/3144 nt) acrB11 Cation/multidrug efflux pump
?AM260479 = 3875215 9 (0.370)coding (2337/3144 nt) acrB11 Cation/multidrug efflux pump
* ? AM260479 = 401411920 (0.750)7 (0.280) 7/198 1.3 23.9% coding (2400/3198 nt) acrB7a Cation/multidrug efflux pump
?AM260479 = 4014127 26 (1.060)coding (2408/3198 nt) acrB7a Cation/multidrug efflux pump
* ? AM260480 203141 =23 (0.780)5 (0.180) 5/204 2.0 21.8% coding (1119/1413 nt) h16_B0179 Hemagglutinin‑related transmembrane protein
?AM260480 203228 = 14 (0.500)coding (1032/1413 nt) h16_B0179 Hemagglutinin‑related transmembrane protein
* ? AM260480 = 34462135 (1.190)9 (0.370) 8/178 1.2 25.7% coding (922/984 nt) h16_B0308 transcriptional regulator, AraC‑family
?AM260480 = 344603 23 (0.940)coding (904/984 nt) h16_B0308 transcriptional regulator, AraC‑family
* ? AM260480 = 188552122 (0.750)8 (0.290) 7/198 1.5 31.2% coding (74/1026 nt) h16_B1674 hypothetical membrane spanning protein
?AM260480 = 1885547 15 (0.550)coding (100/1026 nt) h16_B1674 hypothetical membrane spanning protein
* ? AM260480 = 200788717 (0.580)6 (0.210)
+GC
4/210 2.3 20.6% coding (105/918 nt) h16_B1766 transcriptional regulator, LysR‑family
?AM260480 2007935 = 30 (1.020)coding (57/918 nt) h16_B1766 transcriptional regulator, LysR‑family
* ? AM260480 2658131 =16 (0.540)4 (0.150) 4/198 2.2 20.6% intergenic (‑101/‑256) h16_B2348/h16_B2349 response regulator containing sigma54 activator and Fis‑type DNA‑binding domains/response regulator‑like protein containing sigma54 activator and Fis‑type DNA‑binding domains
?AM260480 2658157 = 16 (0.590)intergenic (‑127/‑230) h16_B2348/h16_B2349 response regulator containing sigma54 activator and Fis‑type DNA‑binding domains/response regulator‑like protein containing sigma54 activator and Fis‑type DNA‑binding domains
* ? AY305378 = 44927513 (0.690)4 (0.230) 4/200 1.4 25.7% coding (1249/1461 nt) cbbLp ribulose‑1,5‑bisphosphate carboxylase/oxygenase large subunit
?AY305378 = 449286 11 (0.620)coding (1238/1461 nt) cbbLp ribulose‑1,5‑bisphosphate carboxylase/oxygenase large subunit