breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 46,798 A→G 10.7% D331G (GAT→GGT)  Y75_RS00235 → MFS transporter
RA 65,583 T→G 8.1% T66T (ACA→ACC polB ← DNA polymerase II
RA 65,670 T→G 17.0% A37A (GCA→GCC polB ← DNA polymerase II
RA 99,078 A→T 9.1% I226F (ATC→TTC)  ftsW → cell division protein FtsW
RA 109,454 T→G 9.0% G392G (GGT→GGG secA → preprotein translocase subunit SecA
RA 161,619 A→G 15.5% I138T (ATC→ACC)  thpR ← RNA 2',3'‑cyclic phosphodiesterase
RA 228,688 Δ1 bp 100% intergenic (+9/‑70) Y75_RS01035 → / → rrf 23S ribosomal RNA/5S ribosomal RNA
RA 249,538 T→G 28.1% H187P (CAC→CCC)  flhA ← flagellar type III secretion system protein FlhA
RA 282,321 T→C 12.3% S274P (TCC→CCC)  yagE → 2‑keto‑3‑deoxygluconate aldolase
RA 359,586 T→G 18.9% V133G (GTG→GGG)  cynX → cyanate transporter CynX
RA 432,873 T→G 25.8% G65G (GGT→GGG ribD → bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase RibD
RA 438,431 A→T 20.3% I324N (ATC→AAC)  dxs ← 1‑deoxy‑D‑xylulose‑5‑phosphate synthase
RA 438,444 T→C 15.9% S320G (AGC→GGC)  dxs ← 1‑deoxy‑D‑xylulose‑5‑phosphate synthase
RA 463,430 T→G 5.5% Y90* (TAT→TAG Y75_RS02275 → hypothetical protein
RA 547,694 A→G 100% E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,831:1 +G 100% coding (251/261 nt) Y75_RS02655 → hypothetical protein
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
MC JC 654,214 Δ1,199 bp 100% Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
RA 696,963 T→G 9.1% intergenic (‑37/+1) Y75_RS03440 ← / ← Y75_RS03445 tRNA‑Gln/tRNA‑Gln
JC 705,229 +CCG 95.0% coding (864/1947 nt) nagE → PTS N‑acetyl glucosamine transporter subunit IIABC
RA 949,176 G→T 18.9% P272Q (CCA→CAA)  Y75_RS04685 ← LysR family transcriptional regulator
RA 954,796 C→G 15.6% P31P (CCG→CCC focA ← formate transporter FocA
RA 954,805 C→T 11.1% T28T (ACG→ACA focA ← formate transporter FocA
RA 954,815 T→A 17.2% Y25F (TAT→TTT)  focA ← formate transporter FocA
RA 1,065,700 C→T 100% R415C (CGT→TGT)  Y75_RS05220 → YccE family protein
RA 1,093,686 T→C 100% V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
RA 1,303,712 A→G 100% S273G (AGC→GGC)  oppA → oligopeptide ABC transporter substrate‑binding protein OppA
MC JC 1,413,639 Δ23,060 bp 100% [ttcA]Y75_RS24865 33 genes
RA 1,531,018 A→C 12.8% H389P (CAC→CCC)  Y75_RS07695 → RHS repeat protein
RA 1,703,339 A→G 10.4% G223G (GGA→GGG malY → bifunctional maltose regulon transcriptional repressor/cystathionine beta‑lyase MalY
RA 1,710,438 G→A 11.2% K602K (AAG→AAA rsxC → electron transport complex subunit RsxC
RA 1,710,440 T→G 7.3% L603R (CTG→CGG)  rsxC → electron transport complex subunit RsxC
RA 1,739,298 A→G 100% intergenic (‑39/‑260) ynhF ← / → purR stress response protein YnhF/HTH‑type transcriptional repressor PurR
RA 1,892,079 T→A 91.1% A56A (GCA→GCT yeaY ← Slp family lipoprotein YeaY
RA 2,152,044 A→C 16.8% V360G (GTG→GGG)  yegI ← protein kinase YegI
MC JC 2,320,795 Δ1,336 bp 100% Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,362,339 C→A 10.2% Q256K (CAA→AAA)  Y75_RS11795 → ISNCY family transposase
RA 2,366,007 A→C 14.1% G26G (GGT→GGG rhmD ← L‑rhamnonate dehydratase
RA 2,530,688 C→A 10.2% T39N (ACC→AAC)  Y75_RS12650 → outer membrane protein
RA 2,792,331 T→G 8.6% V314G (GTG→GGG)  Y75_RS13895 → 4‑aminobutyrate‑‑2‑oxoglutarate transaminase
RA 2,797,637 C→A 18.0% intergenic (‑486/‑183) stpA ← / → alaE DNA‑binding protein StpA/L‑alanine exporter AlaE
RA 2,824,894 A→C 14.5% N136T (AAC→ACC)  srlA → PTS glucitol/sorbitol transporter subunit IIC
RA 2,866,109 C→A 100% pseudogene (99/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 2,877,643 G→T 13.1% A262A (GCC→GCA cas1e ← type I‑E CRISPR‑associated endonuclease Cas1
RA 2,906,975 C→G 100% D450H (GAC→CAC)  pyrG ← CTP synthase (glutamine hydrolyzing)
RA 2,910,761 A→G 100% L516P (CTG→CCG)  Y75_RS14515 ← GTP pyrophosphokinase
RA 2,973,749 T→G 18.5% T308P (ACT→CCT)  aas ← bifunctional acyl‑ACP‑‑phospholipid O‑acyltransferase/long‑chain‑fatty‑acid‑‑ACP ligase
RA 3,063,461 G→A 11.4% E2K (GAA→AAA)  scpC → propionyl‑CoA:succinate CoA transferase
RA 3,205,094 G→A 15.4% intergenic (‑182/‑25) ttdR ← / → ttdA DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
RA 3,205,127 C→G 8.9% S3R (AGC→AGG ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,132 G→A 11.6% S5N (AGT→AAT)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,140 C→A 15.8% Q8K (CAA→AAA)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,143 C→A 22.4% Q9K (CAG→AAG)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,221,478 A→C 10.6% T64P (ACC→CCC)  ebgA → beta‑galactosidase subunit alpha
RA 3,448,513 G→A 91.0% A940V (GCG→GTG)  rpoC ← DNA‑directed RNA polymerase subunit beta'
RA 3,493,183 T→C 12.2% G323G (GGA→GGG Y75_RS17350 ← PTS fructose transporter subunit EIIC
RA 3,614,369 A→C 10.2% V32G (GTA→GGA)  tatB ← Sec‑independent protein translocase subunit TatB
RA 3,645,110 A→T 100% L140Q (CTG→CAG)  cyaA ← class I adenylate cyclase
RA 3,656,455 C→T 100% V91I (GTT→ATT)  rffM ← lipopolysaccharide N‑acetylmannosaminouronosyltransferase
RA 3,661,298 T→G 10.4% T80P (ACC→CCC)  rffA ← dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA 3,741,125 A→G 19.1% M352V (ATG→GTG)  Y75_RS18545 → adenine permease AdeP
RA 3,741,129 T→G 17.7% V353G (GTG→GGG)  Y75_RS18545 → adenine permease AdeP
RA 3,741,162 A→T 9.8% Y364F (TAC→TTC)  Y75_RS18545 → adenine permease AdeP
MC JC 3,742,936 Δ1,336 bp 100% Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
MC JC 3,746,911 Δ1,199 bp 100% Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
RA 3,764,882 A→C 6.5% H32Q (CAT→CAG Y75_RS18670 ← hypothetical protein
RA 3,764,886 G→C 8.7% S31C (TCC→TGC)  Y75_RS18670 ← hypothetical protein
RA 3,764,893 G→C 8.0% L29V (CTG→GTG)  Y75_RS18670 ← hypothetical protein
RA 3,775,819 A→G 6.2% L233P (CTC→CCC)  Y75_RS18730 ← GntR family transcriptional regulator
RA 3,777,745 A→T 29.3% I312F (ATT→TTT)  Y75_RS18735 → PTS transporter subunit EIIC
RA 3,777,753 A→C 21.8% A314A (GCA→GCC Y75_RS18735 → PTS transporter subunit EIIC
RA 3,777,754 A→C 34.0% T315P (ACC→CCC)  Y75_RS18735 → PTS transporter subunit EIIC
RA 3,777,758 T→A 10.0% L316* (TTA→TAA)  Y75_RS18735 → PTS transporter subunit EIIC
RA 3,797,639 C→G 6.7% G338G (GGC→GGG Y75_RS18845 → adenine permease AdeQ
RA 3,810,059 A→T 9.8% intergenic (‑101/+20) Y75_RS18905 ← / ← xanP AsmA family protein/xanthine/proton symporter XanP
RA 3,810,061 C→T 13.1% intergenic (‑103/+18) Y75_RS18905 ← / ← xanP AsmA family protein/xanthine/proton symporter XanP
RA 3,810,064 A→T 19.5% intergenic (‑106/+15) Y75_RS18905 ← / ← xanP AsmA family protein/xanthine/proton symporter XanP
RA 3,814,325 A→C 7.3% V294G (GTG→GGG)  recG ← ATP‑dependent DNA helicase RecG
RA 3,819,310 T→C 9.7% C22C (TGT→TGC ligB → NAD‑dependent DNA ligase LigB
RA 3,821,079 C→A 6.5% A155S (GCC→TCC)  Y75_RS18950 ← trimeric intracellular cation channel family protein
RA 3,824,921 A→C 7.5% D92A (GAC→GCC)  pyrE → orotate phosphoribosyltransferase
RA 3,849,617 C→G 6.5% A183G (GCG→GGG)  tdh → L‑threonine 3‑dehydrogenase
RA 3,857,203 A→T 6.3% L150F (TTA→TTT gpsA → NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
RA 3,857,518 C→A 8.2% D255E (GAC→GAA gpsA → NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
RA 3,858,449 T→G 15.6% G199G (GGT→GGG cysE → serine O‑acetyltransferase
RA 3,858,461 C→G 9.2% G203G (GGC→GGG cysE → serine O‑acetyltransferase
RA 3,878,107 T→C 15.6% G42G (GGA→GGG Y75_RS19225 ← RHS element protein RhsA
RA 3,880,022 T→G 12.9% V286G (GTC→GGC)  selA → L‑seryl‑tRNA(Sec) selenium transferase
RA 3,880,031 T→G 20.9% V289G (GTG→GGG)  selA → L‑seryl‑tRNA(Sec) selenium transferase
RA 3,880,033 A→G 23.3% S290G (AGT→GGT)  selA → L‑seryl‑tRNA(Sec) selenium transferase
RA 3,880,074 A→G 16.5% G303G (GGA→GGG selA → L‑seryl‑tRNA(Sec) selenium transferase
RA 3,895,373 T→G 26.4% D80A (GAC→GCC)  yiaN ← 2,3‑diketo‑L‑gulonate transporter large permease YiaN
RA 3,908,677 T→G 16.4% N203T (AAC→ACC)  Y75_RS19370 ← D‑xylose ABC transporter substrate‑binding protein
RA 3,908,712 G→A 7.2% G191G (GGC→GGT Y75_RS19370 ← D‑xylose ABC transporter substrate‑binding protein
RA 4,096,128 T→G 15.7% V186G (GTC→GGC)  bioH → pimeloyl‑ACP methyl ester esterase BioH
RA 4,200,347 T→A 100% W321R (TGG→AGG)  rpoA → DNA‑directed RNA polymerase subunit alpha
RA 4,262,217 A→C 12.5% T296P (ACC→CCC)  dinF → MATE family efflux transporter DinF
RA 4,348,548 A→T 10.9% N164Y (AAT→TAT)  melB → melibiose:sodium transporter MelB
RA 4,371,271 Δ2 bp 100% pseudogene (182‑183/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter
RA 4,497,444 A→T 10.2% T196T (ACT→ACA idnO ← gluconate 5‑dehydrogenase
RA 4,611,582 A→G 21.5% S267P (TCC→CCC)  rsmC ← 16S rRNA (guanine(1207)‑N(2))‑methyltransferase RsmC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 573976–575013 581786 6774–7811 50 [49] [0] 135 [Y75_RS02845]–[Y75_RS02900] [Y75_RS02845],Y75_RS02850,essD,Y75_RS02860,rzoD,bor,Y75_RS02875,Y75_RS02880,Y75_RS02885,Y75_RS02890,Y75_RS02895,[Y75_RS02900]
* * ÷ NC_007779 1301041–1302370 1302407 38–1367 132 [0] [0] 99 Y75_RS06530–Y75_RS06530 Y75_RS06530,Y75_RS06530
* * ÷ NC_007779 1397877–1398957 1406267 7311–8391 51 [48] [0] 113 [Y75_RS07025]–[Y75_RS07065] [Y75_RS07025],Y75_RS07030,uspE,Y75_RS07040,ogt,abgT,Y75_RS07060,[Y75_RS07065]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 273180 =NA (NA)125 (0.840) 77/402 NT 100% intergenic (+3/+145) Y75_RS01330/Y75_RS01335 hypothetical protein/IS5‑like element IS5 family transposase
?NC_007779 701988 = 0 (0.000)coding (29/1221 nt) nagC DNA‑binding transcriptional regulator NagC
* ? NC_007779 567950 =55 (0.370)4 (0.030) 4/380 NT 8.6% coding (33/150 nt) Y75_RS24780 hypothetical protein
?NC_007779 568069 = 33 (0.240)intergenic (+2/‑56) Y75_RS24780/Y75_RS02795 hypothetical protein/recombinase family protein
* ? NC_007779 = 575008NA (NA)134 (0.900) 83/404 NT 100% coding (1041/1068 nt) Y75_RS02850 porin
?NC_007779 581787 = 0 (0.000)coding (215/627 nt) Y75_RS02900 class I SAM‑dependent methyltransferase
* ? NC_007779 = 689467NA (NA)137 (0.920) 79/404 NT 100% intergenic (‑32/+298) Y75_RS03405/lnt IS5‑like element IS5 family transposase/apolipoprotein N‑acyltransferase
?NC_007779 = 701992 0 (0.000)coding (25/1221 nt) nagC DNA‑binding transcriptional regulator NagC
* ? NC_007779 1106360 =NA (NA)16 (0.110) 4/398 NT 55.5% coding (187/914 nt) Y75_RS05455 IS3‑like element IS2 family transposase
?NC_007779 1106752 = 13 (0.090)coding (155/361 nt) Y75_RS05455 IS3‑like element IS2 family transposase
* ? NC_007779 1209367 =70 (0.470)78 (0.570) 54/372 NT 58.8% coding (290/630 nt) Y75_RS06020 hypothetical protein
?NC_007779 1211196 = 45 (0.330)intergenic (‑6/‑66) Y75_RS06035/pinE tail fiber protein/site‑specific DNA recombinase
* ? NC_007779 = 120938271 (0.480)71 (0.520) 50/372 NT 56.3% coding (305/630 nt) Y75_RS06020 hypothetical protein
?NC_007779 = 1211179 45 (0.330)coding (12/495 nt) Y75_RS06035 tail fiber protein
* ? NC_007779 1294844 =0 (0.000)152 (1.020) 85/402 NT 100% intergenic (‑345/‑260) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 = 1398951 NA (NA)intergenic (+145/‑128) Y75_RS07025/Y75_RS07030 IS5‑like element IS5 family transposase/DUF2534 family protein
* ? NC_007779 = 12948480 (0.000)163 (1.090) 91/404 NT 100% intergenic (‑349/‑256) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 1397758 = NA (NA)intergenic (‑122/‑32) ynaI/Y75_RS07025 low conductance mechanosensitive channel YnaI/IS5‑like element IS5 family transposase
* ? NC_007779 = 13010400 (0.000)92 (0.720)
+28 bp
66/348 NT 100% intergenic (+218/‑45) ychE/Y75_RS06530 NAAT family transporter YchE/IS3‑like element IS2 family transposase
?NC_007779 = 4311564 NA (NA)pseudogene (914/921 nt) alsK allose kinase
* ? NC_007779 1302408 =0 (0.000)93 (0.620) 59/404 NT 100% intergenic (+50/‑488) Y75_RS06530/oppA IS3‑like element IS2 family transposase/oligopeptide ABC transporter substrate‑binding protein OppA
?NC_007779 1397758 = NA (NA)intergenic (‑122/‑32) ynaI/Y75_RS07025 low conductance mechanosensitive channel YnaI/IS5‑like element IS5 family transposase
* ? NC_007779 1406268 =0 (0.000)111 (0.740) 72/404 NT 100% coding (12/1311 nt) Y75_RS07065 p‑aminobenzoyl‑glutamate hydrolase subunit AbgA
?NC_007779 1980209 = NA (NA)intergenic (‑298/+146) flhD/Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
* ? NC_007779 1885208 =0 (0.000)124 (0.830) 75/404 NT 100% coding (307/1446 nt) Y75_RS09485 BCCT family transporter
?NC_007779 2411458 = NA (NA)intergenic (‑147/+15) lrhA/Y75_RS12035 transcriptional regulator LrhA/IS1‑like element IS1A family transposase
* ? NC_007779 = 18852153 (0.020)139 (0.930) 87/404 NT 97.9% coding (314/1446 nt) Y75_RS09485 BCCT family transporter
?NC_007779 = 2412225 NA (NA)intergenic (‑55/‑782) Y75_RS12035/Y75_RS12040 IS1‑like element IS1A family transposase/alanine transaminase AlaA
* ? NC_007779 = 21769820 (0.000)165 (1.130) 88/394 NT 100% intergenic (‑2/‑33) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
?NC_007779 2178182 = 0 (0.000)pseudogene (202/207 nt) gatA PTS galactitol transporter subunit IIA
* ? NC_007779 2380495 =16 (0.110)89 (0.600) 55/400 NT 84.9% coding (138/963 nt) menC o‑succinylbenzoate synthase
?NC_007779 2519723 = NA (NA)intergenic (+33/‑54) nupC/Y75_RS12570 nucleoside permease NupC/IS4‑like element IS421 family transposase
* ? NC_007779 = 238050612 (0.080)149 (1.010) 83/398 NT 92.6% coding (127/963 nt) menC o‑succinylbenzoate synthase
?NC_007779 = 2521059 NA (NA)intergenic (+170/+30) Y75_RS12570/Y75_RS12575 IS4‑like element IS421 family transposase/sensor domain‑containing phosphodiesterase
* ? NC_007779 = 32611330 (0.000)158 (1.080) 73/396 NT 100% intergenic (‑59/‑33) tdcE/Y75_RS16205 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase/IS5‑like element IS5 family transposase
?NC_007779 3262333 = 0 (0.000)pseudogene (1183/1185 nt) tdcD propionate kinase
* ? NC_007779 3748316 =0 (0.000)346 (2.320) 144/404 NT 100% pseudogene (665/873 nt) tnaB low affinity tryptophan permease TnaB
?NC_007779 = 4056987 NA (NA)coding (391/417 nt) Y75_RS20060 hypothetical protein
* ? NC_007779 = 374832415 (0.100)127 (0.850) 67/404 NT 89.4% pseudogene (657/873 nt) tnaB low affinity tryptophan permease TnaB
?NC_007779 4056220 = NA (NA)intergenic (‑209/+15) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
* ? NC_007779 = 37505900 (0.000)443 (3.030) 132/396 NT 100% intergenic (‑104/‑33) tnaA/Y75_RS18600 tryptophanase/IS5‑like element IS5 family transposase
?NC_007779 3751790 = 0 (0.000)intergenic (+151/+116) Y75_RS18600/tnaC IS5‑like element IS5 family transposase/tryptophanase leader peptide
* ? NC_007779 = 3949333155 (1.040)168 (1.130) 88/404 NT 52.0% coding (1526/2340 nt) bcsB cellulose biosynthesis cyclic di‑GMP‑binding regulatory protein BcsB
?NC_007779 4056220 = NA (NA)intergenic (‑209/+15) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
* ? NC_007779 4056221 =NA (NA)132 (0.890) 84/402 NT 96.4% intergenic (‑210/+14) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
?NC_007779 4370787 = 5 (0.030)pseudogene (667/1304 nt) Y75_RS21600 anaerobic C4‑dicarboxylate transporter
* ? NC_007779 = 4056987NA (NA)176 (1.180) 100/404 NT 98.9% coding (391/417 nt) Y75_RS20060 hypothetical protein
?NC_007779 = 4370795 2 (0.010)pseudogene (659/1304 nt) Y75_RS21600 anaerobic C4‑dicarboxylate transporter
* ? NC_007779 = 43103620 (0.000)146 (1.000) 64/396 NT 100% intergenic (‑286/‑33) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 4311562 = 0 (0.000)pseudogene (916/921 nt) alsK allose kinase