breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 9,907 A→C 5.8% intergenic (+14/+21) mog → / ← satP molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter
RA 9,908 G→T 5.5% intergenic (+15/+20) mog → / ← satP molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter
RA 83,587 A→C 5.6% intergenic (‑58/+35) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 83,590 G→T 5.1% intergenic (‑61/+32) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 164,620 C→T 5.7% intergenic (+86/‑110) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 238,532 C→A 8.7% V69L (GTA→TTA)  ykfM ← lethality reduction protein, putative inner membrane protein
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 357,606 C→T 5.1% intergenic (+152/+185) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 357,775 A→T 6.1% intergenic (+321/+16) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 361,323 G→C 13.3% R180G (CGG→GGG)  lacA ← thiogalactoside acetyltransferase
RA 518,334 T→G 7.2% intergenic (‑55/+6) ybbN ← / ← ybbO DnaK co‑chaperone, thioredoxin‑like protein/putative oxidoreductase
RA 543,091 G→C 5.3% intergenic (+58/+170) glxK → / ← allE glycerate kinase II/S‑ureidoglycine aminohydrolase
RA 543,094 G→C 5.2% intergenic (+61/+167) glxK → / ← allE glycerate kinase II/S‑ureidoglycine aminohydrolase
RA 681,700 T→A 5.3% intergenic (+37/+23) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
RA 702,330 Δ1 bp 10.9% coding (422/1149 nt) nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 710,170 C→T 7.6% intergenic (+54/+30) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 710,173 A→G 7.6% intergenic (+57/+27) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 720,061 C→T 5.1% G134S (GGC→AGC)  speF ← ornithine decarboxylase isozyme, inducible
RA 781,269 C→T 5.5% intergenic (+47/‑100) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 781,668 C→T 7.2% intergenic (+16/‑417) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A
RA 781,670 A→T 7.3% intergenic (+18/‑415) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A
RA 781,672 A→G 7.0% intergenic (+20/‑413) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A
RA 832,378 G→T 5.2% intergenic (+142/+90) rhlE → / ← ybiA ATP‑dependent RNA helicase/DUF1768 family protein
RA 832,379 C→T 5.2% intergenic (+143/+89) rhlE → / ← ybiA ATP‑dependent RNA helicase/DUF1768 family protein
RA 850,993 T→C 5.5% intergenic (+28/+21) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 850,995 T→G 5.4% intergenic (+30/+19) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 850,996 C→A 5.3% intergenic (+31/+18) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 850,998 G→A 5.5% intergenic (+33/+16) ompX → / ← opgE outer membrane protein X/OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 922,314:1 +C 5.8% intergenic (+21/+52) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
RA 1,018,983 A→T 7.4% intergenic (+46/+30) matP → / ← ompA Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
RA 1,018,986 A→T 8.0% intergenic (+49/+27) matP → / ← ompA Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
RA 1,151,874 T→A 5.1% intergenic (+23/‑65) acpP → / → fabF acyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
RA 1,226,115 A→C 6.6% intergenic (‑35/‑485) minC ← / → ycgJ inhibitor of FtsZ ring polymerization/uncharacterized protein
JC JC 1,293,032 IS1 (+) +9 bp 10.0% intergenic (‑110/‑487) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,351,228 G→C 13.9% intergenic (‑189/+179) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,388,283 C→T 6.4% intergenic (+22/+22) tyrR → / ← tpx aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase
RA 1,388,284 A→T 6.7% intergenic (+23/+21) tyrR → / ← tpx aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase
RA 1,388,285 A→G 6.7% intergenic (+24/+20) tyrR → / ← tpx aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase
RA 1,466,144 G→T 5.3% pseudogene (753/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,201 T→C 7.5% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,345 T→C 5.2% pseudogene (954/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,495 C→T 7.5% pseudogene (1104/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,604 G→A 5.2% pseudogene (1213/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,708,814 A→T 7.8% A632A (GCA→GCT rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,864,768 T→C 5.0% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 1,985,112 C→G 5.5% intergenic (‑43/+27) araG ← / ← araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
RA 2,028,158 G→C 5.8% intergenic (‑141/+30) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein
RA 2,028,159 T→A 5.3% intergenic (‑142/+29) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein
RA 2,028,160 G→C 5.7% intergenic (‑143/+28) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein
RA 2,167,278 G→A 5.4% intergenic (+78/+24) cyaR → / ← ogrK sRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth
RA 2,167,279 A→G 5.4% intergenic (+79/+23) cyaR → / ← ogrK sRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth
RA 2,167,280 C→T 5.4% intergenic (+80/+22) cyaR → / ← ogrK sRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth
RA 2,167,281 T→C 5.4% intergenic (+81/+21) cyaR → / ← ogrK sRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,177,234 C→A 16.6% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,177,245 G→T 15.3% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,229,177 A→C 16.4% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C
RA 2,304,649 C→T 7.0% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,013 A→G 5.3% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,085 A→G 6.4% intergenic (+692/+23) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,086 C→G 6.4% intergenic (+693/+22) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,087 C→T 6.4% intergenic (+694/+21) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,390,012 Δ1 bp 7.5% intergenic (+48/+36) yfbP → / ← nuoN TPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N
RA 2,390,016:1 +C 7.5% intergenic (+52/+32) yfbP → / ← nuoN TPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N
RA 2,428,697 A→G 5.7% intergenic (‑71/+24) ubiX ← / ← purF 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase/amidophosphoribosyltransferase
RA 2,428,699 A→T 5.7% intergenic (‑73/+22) ubiX ← / ← purF 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase/amidophosphoribosyltransferase
RA 2,428,701 C→T 5.8% intergenic (‑75/+20) ubiX ← / ← purF 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase/amidophosphoribosyltransferase
RA 2,470,427 C→A 19.6% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,470,431 G→T 15.3% R434I (AGA→ATA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,618,945 A→T 5.6% F406I (TTC→ATC)  uraA ← uracil permease
RA 2,618,946 G→C 5.4% I405M (ATC→ATG) ‡ uraA ← uracil permease
RA 2,618,947 A→T 5.6% I405N (ATC→AAC) ‡ uraA ← uracil permease
RA 2,653,367 G→A 7.9% intergenic (+28/‑488) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
RA 2,653,372 T→C 7.7% intergenic (+33/‑483) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
RA 2,662,480 A→T 7.1% intergenic (‑349/+103) iscR ← / ← trmJ isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
RA 2,753,986 A→T 5.3% intergenic (+40/+22) bamE → / ← ratB lipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein
RA 2,778,123 G→T 6.1% intergenic (+341/+23) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,778,124 A→C 6.4% intergenic (+342/+22) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,817,916 T→G 7.2% intergenic (‑56/+143) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,818,046 A→G 5.5% intergenic (‑186/+13) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,818,158 T→A 5.5% intergenic (‑23/+40) argZ ← / ← argY tRNA‑Arg/tRNA‑Arg
RA 2,900,322 C→T 5.7% intergenic (‑49/‑270) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,900,324 G→T 5.8% intergenic (‑51/‑268) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,900,327 A→C 6.2% intergenic (‑54/‑265) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,972,652 A→T 5.9% intergenic (+15/+17) tas → / ← lplT putative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter
RA 2,972,653 A→T 6.1% intergenic (+16/+16) tas → / ← lplT putative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter
RA 3,004,963 T→A 10.3% E275V (GAG→GTG)  ygeV ← putative sigma‑54‑interacting transcriptional activator
RA 3,046,050 T→G 9.9% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,054,888 A→T 6.2% V186E (GTA→GAA)  ygfB ← UPF0149 family protein
RA 3,087,396 T→G 5.2% F231V (TTC→GTC)  metK → S‑adenosylmethionine synthetase
RA 3,156,590 C→A 7.1% intergenic (+72/‑33) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,156,591 T→G 7.4% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,239,609 T→G 7.9% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,239,678 T→A 7.3% intergenic (+133/‑266) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,269,956 T→C 6.3% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,316,740 A→G 5.3% S263P (TCT→CCT)  nusA ← transcription termination/antitermination L factor
RA 3,324,952 A→G 6.8% intergenic (‑41/+49) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,955 A→G 6.6% intergenic (‑44/+46) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,956 C→T 5.7% intergenic (‑45/+45) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,959 C→T 6.3% intergenic (‑48/+42) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,332,822 G→A 5.7% intergenic (‑87/+40) yhbE ← / ← rpmA EamA family inner membrane putative transporter/50S ribosomal subunit protein L27
RA 3,332,823 T→C 5.7% intergenic (‑88/+39) yhbE ← / ← rpmA EamA family inner membrane putative transporter/50S ribosomal subunit protein L27
RA 3,334,704 G→C 6.9% intergenic (+23/‑205) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator
RA 3,334,705 C→T 6.3% intergenic (+24/‑204) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator
RA 3,377,768 C→T 6.0% intergenic (‑348/+47) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
RA 3,377,769 G→T 6.2% intergenic (‑349/+46) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
RA 3,377,770 A→C 5.9% intergenic (‑350/+45) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
RA 3,377,771 A→G 5.9% intergenic (‑351/+44) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
RA 3,423,531 T→G 6.3% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,458,110 C→A 100% T578T (ACC→ACA gspD → general secretory pathway component, cryptic
RA 3,482,325 G→A 100% R346H (CGC→CAC)  yheS → ABC‑F family protein predicted regulatory ATPase
RA 3,554,283 T→A 5.4% L400Q (CTG→CAG)  malT → mal regulon transcriptional activator
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,608,695 C→G 8.2% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,608,696 T→G 5.3% intergenic (+47/+55) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,642,358 T→A 5.7% intergenic (+27/+22) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
RA 3,642,361 T→A 5.2% intergenic (+30/+19) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
RA 3,708,427 G→A 5.4% intergenic (‑722/+189) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,708,428 T→A 5.7% intergenic (‑723/+188) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,708,429 T→C 6.7% intergenic (‑724/+187) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,728,125 T→G 7.9% Y416S (TAC→TCC)  xylB ← xylulokinase
RA 3,781,022 T→G 7.4% intergenic (+5/‑193) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,131 T→G 6.3% intergenic (+114/‑84) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,158 A→C 9.2% intergenic (+141/‑57) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,810,321 T→C 5.9% intergenic (+17/+22) coaD → / ← mutM pantetheine‑phosphate adenylyltransferase/formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase
RA 3,810,324 G→A 5.5% intergenic (+20/+19) coaD → / ← mutM pantetheine‑phosphate adenylyltransferase/formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,828,980 A→C 9.2% Q12H (CAA→CAC xanP → xanthine permease
RA 3,891,502 C→G 6.2% intergenic (+19/‑113) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,504 T→A 6.2% intergenic (+21/‑111) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,506 C→G 6.3% intergenic (+23/‑109) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,959,481 C→G 7.2% intergenic (+36/+51) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,515 T→A 9.8% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 4,086,965 G→T 6.8% intergenic (+116/‑37) fdhD → / → yiiG formate dehydrogenase formation protein/DUF3829 family lipoprotein
RA 4,171,872 A→C 6.0% intergenic (+116/‑185) rrfB → / → murB 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
RA 4,296,060 C→T 36.8% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,362,528 G→A 5.5% intergenic (‑175/+23) yjdQ ← / ← pheU pseudogene, P4‑like integrase remnant/tRNA‑Phe
RA 4,362,529 T→C 5.5% intergenic (‑176/+22) yjdQ ← / ← pheU pseudogene, P4‑like integrase remnant/tRNA‑Phe
RA 4,473,927 C→A 100% A43S (GCC→TCC)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,520,500 Δ1 bp 6.0% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,520,504:1 +T 5.7% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,614,378 A→G 7.2% intergenic (+118/+302) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 448603 450707 2105 134 [132] [128] 134 [cyoC]–[cyoA] [cyoC],cyoB,[cyoA]
* * ÷ NC_000913 2403875 2404668 794 134 [133] [130] 134 nuoB–[nuoA] nuoB,[nuoA]
* * ÷ NC_000913 3423722–3424558 3424558 1–837 139 [133] [133] 134 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 274384 =NA (NA)6 (0.030) 3/272 NT NA noncoding (766/1195 nt) IS5 repeat region
?NC_000913 274422 = NA (NA)noncoding (728/1195 nt) IS5 repeat region
* ? NC_000913 = 274624NA (NA)3 (0.010) 3/272 NT NA noncoding (526/1195 nt) IS5 repeat region
?NC_000913 = 274693 NA (NA)noncoding (457/1195 nt) IS5 repeat region
* ? NC_000913 = 274695NA (NA)4 (0.020) 3/270 NT NA noncoding (455/1195 nt) IS5 repeat region
?NC_000913 = 274727 NA (NA)noncoding (423/1195 nt) IS5 repeat region
* ? NC_000913 381663 =NA (NA)3 (0.010) 3/264 NT NA noncoding (404/1331 nt) IS2 repeat region
?NC_000913 381703 = NA (NA)noncoding (444/1331 nt) IS2 repeat region
* ? NC_000913 381681 =NA (NA)4 (0.020) 3/260 NT NA noncoding (422/1331 nt) IS2 repeat region
?NC_000913 381753 = NA (NA)noncoding (494/1331 nt) IS2 repeat region
* ? NC_000913 = 699522NA (NA)3 (0.010) 3/264 NT NA noncoding (98/98 nt) RIP61 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP61 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 = 3706035 NA (NA)noncoding (73/98 nt) RIP266 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP266 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 781369 =205 (0.840)30 (0.130)
+GAAG
22/270 NT 14.8% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 151 (0.620)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 781577 =170 (0.700)50 (0.210)
+GAAG
3/270 NT 24.3% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 151 (0.620)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 937227NA (NA)5 (0.020) 4/262 NT NA noncoding (4/36 nt) REP85 (repetitive extragenic palindromic) element; contains 1 REP sequences REP85 (repetitive extragenic palindromic) element; contains 1 REP sequences
?NC_000913 = 937259 NA (NA)noncoding (36/36 nt) REP85 (repetitive extragenic palindromic) element; contains 1 REP sequences REP85 (repetitive extragenic palindromic) element; contains 1 REP sequences
* ? NC_000913 1207790 =114 (0.470)109 (0.500) 65/246 NT 53.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 92 (0.430)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805106 (0.430)129 (0.600) 61/246 NT 58.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 92 (0.430)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 128708490 (0.370)10 (0.050) 5/238 NT 7.7% noncoding (153/171 nt) rttR rtT sRNA, processed from tyrT transcript
?NC_000913 1287557 = 163 (0.780)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 1293008134 (0.570)10 (0.040) 8/270 NT 6.9% intergenic (‑86/‑519) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 1293009 =139 (0.570)14 (0.060) 10/276 NT 9.2% intergenic (‑87/‑518) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 1293124 =179 (0.750)10 (0.040) 5/278 NT 5.1% intergenic (‑202/‑403) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1300693 NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 12994980 (0.000)184 (0.780) 104/270 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785020 (0.000)218 (0.950) 104/262 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 2817942138 (0.570)19 (0.090) 14/238 NT 11.4% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?NC_000913 2818492 = 177 (0.850)noncoding (58/77 nt) argV tRNA‑Arg
* ? NC_000913 = 3620350NA (NA)4 (0.020) 3/266 NT 100% coding (1159/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3620425 0 (0.000)coding (1234/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 3943845 =2 (0.010)11 (0.050) 7/264 NT 85.3% noncoding (142/2904 nt) rrlC 23S ribosomal RNA of rrnC operon
?NC_000913 4037656 = NA (NA)noncoding (138/2905 nt) rrlA 23S ribosomal RNA of rrnA operon