breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 48,837 Δ1 bp coding (1069/1863 nt) kefC → K(+) : H(+) antiporter KefC
RA 49,873 A→G E17E (GAA→GAG folA → dihydrofolate reductase
RA 146,550 C→T V49I (GTC→ATC)  panD ← aspartate 1‑decarboxylase proenzyme
RA 158,868 C→T A87T (GCC→ACC)  pcnB ← poly(A) polymerase I
RA 170,285 T→C L170L (TTG→CTG)  fhuC → iron(III) hydroxamate ABC transporter ATP binding subunit
RA 205,987 A→G T288A (ACC→GCC)  dnaE → DNA polymerase III subunit alpha
RA 269,158 C→T intergenic (‑198/+131) ykfA ← / ← perR CP4‑6 prophage; putative GTP‑binding protein YkfA/putative transcriptional regulator PerR
RA 297,700 C→T R31H (CGT→CAT)  yagP ← putative LysR family substrate binding domain‑containing protein YagP
RA 299,053 C→T G628S (GGC→AGC)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 325,767 A→G V494A (GTA→GCA)  betA ← choline dehydrogenase
RA 329,556 A→G T32A (ACG→GCG)  betT → choline:H(+) symporter
RA 338,135 C→T A453V (GCG→GTG)  yahF → putative acyl‑CoA synthetase YahF
RA 342,600 A→G intergenic (+93/‑284) yahJ → / → yahK putative deaminase with metallo‑dependent hydrolase domain/aldehyde reductase, NADPH‑dependent
RA 343,539 C→T A219V (GCC→GTC)  yahK → aldehyde reductase, NADPH‑dependent
RA 360,114 C→T T41T (ACC→ACT cynX → cyanate transporter
RA 375,067 A→G intergenic (+186/‑392) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 375,087 C→T intergenic (+206/‑372) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 385,090 A→G intergenic (‑474/‑142) yaiS ← / → tauA putative deacetylase YaiS/taurine ABC transporter periplasmic binding protein
RA 402,583 C→T G279G (GGC→GGT phoA → alkaline phosphatase
RA 456,497 (T)8→7 intergenic (+66/‑180) tig → / → clpP trigger factor/ATP‑dependent Clp protease proteolytic subunit
RA 465,107 A→G S147S (AGT→AGC queC ← 7‑cyano‑7‑deazaguanine synthase
RA 543,318 A→G R243R (CGT→CGC allE ← (S)‑ureidoglycine aminohydrolase
RA 570,475 A→G intergenic (+47/‑418) ybcK → / → ybcL DLP12 prophage; putative recombinase/DLP12 prophage; periplasmic protein YbcL
RA 576,733 A→G pseudogene (93/1040 nt) nmpC ← DLP12 prophage; putative outer membrane porin NmpC
RA 581,068 C→T P79S (CCA→TCA)  nohD → DLP12 prophage; putative DNA‑packaging protein NohD
RA 588,320 C→T A879T (GCC→ACC)  nfrA ← bacteriophage N4 receptor, outer membrane protein
RA 591,967 C→T M404I (ATG→ATA nfrB ← bacteriophage N4 receptor, inner membrane subunit
RA 621,820 C→T A124T (GCC→ACC)  fepD ← ferric enterobactin ABC transporter membrane subunit FebD
RA 636,230 C→T E114K (GAA→AAA)  ybdN ← putative PAPS reductase/DUF3440 domain‑containing protein YbdN
RA 827,694 A→G Y430H (TAT→CAT)  ybhF ← putative ABC exporter ATP binding subunit
RA 834,929 C→T S620S (AGC→AGT dinG → ATP‑dependent DNA helicase DinG
RA 852,384 C→T A72T (GCG→ACG)  opgE ← phosphoethanolamine transferase
RA 906,867 T→C E472E (GAA→GAG ybjT ← putative NAD(P)‑dependent oxidoreductase YbjT
RA 915,225 (C)5→4 intergenic (‑368/+127) lysO ← / ← aqpZ L‑lysine exporter/water channel AqpZ
RA 974,415 C→T L33L (CTG→TTG)  mukF → chromosome partitioning protein MukF
RA 1,013,461 A→G D68G (GAC→GGC)  pqiB → intermembrane transport protein PqiB
RA 1,057,599 C→T R119Q (CGA→CAA)  torR ← DNA‑binding transcriptional dual regulator TorR
RA 1,097,218 (G)7→6 intergenic (+389/+347) ycdU → / ← serX uncharacterized protein YcdU/tRNA‑Ser
RA 1,142,249 T→C T707A (ACC→GCC)  rne ← ribonuclease E
RA 1,171,768 G→A R733C (CGC→TGC)  mfd ← transcription‑repair coupling factor
RA 1,175,049 T→C L39L (TTA→TTG ycfT ← inner membrane protein YcfT
RA 1,175,732 A→G V102V (GTA→GTG lolC → lipoprotein release complex ‑ inner membrane subunit
RA 1,193,565 T→C H70R (CAC→CGC)  mnmA ← tRNA‑specific 2‑thiouridylase
RA 1,207,235 C→T A108V (GCC→GTC)  ymfQ → e14 prophage; conserved protein YmfQ
RA 1,209,141 T→C pseudogene (515/537 nt) stfE ← e14 prophage; putative side tail fiber protein fragment
RA 1,226,411 (T)6→7 intergenic (‑331/‑189) minC ← / → ycgJ Z‑ring positioning protein MinC/PF05666 family protein YcgJ
RA 1,239,890 A→G T338A (ACG→GCG)  dadX → alanine racemase 2
RA 1,261,695 C→T A61T (GCC→ACC)  prs ← ribose‑phosphate diphosphokinase
RA 1,300,904 A→G intergenic (+357/‑278) insH21 → / → oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
RA 1,342,046 T→C R42R (CGT→CGC pyrF → orotidine‑5'‑phosphate decarboxylase
RA 1,351,196 (G)7→6 intergenic (‑157/+211) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
RA 1,352,436 T→C T67A (ACC→GCC)  sapF ← putrescine ABC exporter ATP binding protein SapF
RA 1,354,511 T→C Y3C (TAC→TGC) 
L320L (TTA→TTG
sapC ←
sapB ←
putrescine ABC exporter membrane protein SapC
putrescine ABC exporter membrane subunit SapB
RA 1,379,443 A→G L520L (CTA→CTG ycjT → kojibiose phosphorylase
RA 1,439,814 T→C D324G (GAC→GGC)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,440,577 C→T A70T (GCT→ACT)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,457,623 A→G T43A (ACC→GCC)  paaF → putative 2,3‑dehydroadipyl‑CoA hydratase
RA 1,474,680 G→A G154S (GGC→AGC)  pdxI → pyridoxine 4‑dehydrogenase
RA 1,479,687 (G)6→7 coding (550/1758 nt) ynbC → hydrolase/methyltransferase domain‑containing protein YnbC
RA 1,482,301 G→A A187V (GCA→GTA)  azoR ← FMN dependent NADH:quinone oxidoreductase
RA 1,555,170 C→T G167D (GGC→GAC)  maeA ← malate dehydrogenase, NAD‑requiring
RA 1,594,132 (C)8→9 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,601,705 C→T G72G (GGC→GGT lsrA → Autoinducer‑2 ABC transporter ATP binding subunit
RA 1,620,807 G→A R111* (CGA→TGA)  eamA ← cysteine/O‑acetylserine exporter EamA
RA 1,640,401 T→C E62G (GAA→GGA)  essQ ← Qin prophage; putative S lysis protein
RA 1,650,575 G→A pseudogene (498/921 nt) ydfE → Qin prophage; protein YdfE
RA 1,686,708 (A)7→6 intergenic (‑120/+23) fumC ← / ← fumA fumarase C/fumarase A
RA 1,710,491 G→A A96A (GCG→GCA rsxG → SoxR [2Fe‑2S] reducing system protein RsxG
RA 1,712,544 G→A intergenic (+386/‑225) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,742,293 T→C V294A (GTA→GCA)  cfa → cyclopropane fatty acyl phospholipid synthase
RA 1,752,145 C→T W562* (TGG→TGA ydhV ← putative oxidoreductase
RA 1,761,354 T→C Q146Q (CAA→CAG sufD ← Fe‑S cluster scaffold complex subunit SufD
RA 1,761,579 T→C P71P (CCA→CCG sufD ← Fe‑S cluster scaffold complex subunit SufD
RA 1,785,435 T→C T560A (ACT→GCT)  ppsA ← phosphoenolpyruvate synthetase
RA 1,789,588 G→T intergenic (+107/‑25) aroH → / → ydiE 3‑deoxy‑7‑phosphoheptulonate synthase, Trp‑sensitive/PF10636 family protein YdiE
RA 1,871,832 (G)6→7 coding (1448/1476 nt) cdgI → putative c‑di‑GMP binding protein CdgI
JC JC 1,873,031 IS1 (–) +9 bp coding (991‑999/1491 nt) dgcJ → putative diguanylate cyclase DgcJ
RA 1,873,800 A→G Q76R (CAG→CGG)  yeaK → mischarged aminoacyl‑tRNA deacylase
RA 1,880,710 G→A A17V (GCC→GTC)  leuE ← leucine exporter
RA 1,938,052 A→G Q135R (CAG→CGG)  pykA → pyruvate kinase II
RA 1,953,492 A→T S17S (TCA→TCT cmoB → tRNA U34 carboxymethyltransferase
RA 1,963,935 G→A I372I (ATC→ATT flhA ← flagellar biosynthesis protein FlhA
RA 1,981,422 G→A A64V (GCC→GTC)  otsA ← trehalose‑6‑phosphate synthase
RA 1,981,526 (C)5→6 coding (87/1425 nt) otsA ← trehalose‑6‑phosphate synthase
RA 2,013,366 (C)5→4 coding (138/1659 nt) fliF → flagellar basal‑body MS‑ring and collar protein
RA 2,027,014 T→C D335G (GAC→GGC)  dgcQ ← putative diguanylate cyclase DgcQ
RA 2,051,895 T→C Y2320H (TAT→CAT)  yeeJ → inverse autotransporter adhesin
RA 2,061,706 T→C E76E (GAA→GAG nac ← DNA‑binding transcriptional dual regulator Nac
RA 2,108,168 A→G G54G (GGT→GGC glf ← UDP‑galactopyranose mutase
RA 2,116,933 A→G P65P (CCT→CCC wcaL ← putative colanic biosynthesis glycosyl transferase
RA 2,130,945 T→C T376A (ACC→GCC)  wcaC ← putative colanic acid biosynthesis glycosyl transferase
RA 2,148,233 A→G D187G (GAC→GGC)  yegD → HSP70 family protein YegD
RA 2,165,573 (C)6→5 intergenic (+52/‑95) yegP → / → yegQ DUF1508 domain‑containing protein YegP/putative peptidase YegQ
RA 2,256,630 A→G F236L (TTC→CTC)  psuT ← putative pseudouridine transporter
RA 2,274,409 C→T G77G (GGC→GGT yejB → putative oligopeptide ABC transporter membrane subunit YejB
RA 2,279,670 Δ1 bp coding (91/1191 nt) bcr ← multidrug efflux pump Bcr
RA 2,314,061 G→A A192T (GCC→ACC)  rcsD → RcsD phosphotransferase
RA 2,350,598 C→T M138I (ATG→ATA glpQ ← glycerophosphoryl diester phosphodiesterase
RA 2,388,781 A→G E49E (GAA→GAG yfbO → uncharacterized protein YfbO
RA 2,401,052 T→C E168E (GAA→GAG nuoF ← NADH:quinone oxidoreductase subunit F
RA 2,457,607 T→C H518R (CAC→CGC)  fadJ ← 3‑hydroxyacyl‑CoA dehydrogenase FadJ
RA 2,469,931 C→T A267A (GCC→GCT yfdI → CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
RA 2,478,945 (G)5→4 coding (1099/1338 nt) dsdX → D‑serine transporter
RA 2,486,481 (A)7→8 coding (2108/3594 nt) evgS → sensory histidine kinase EvgS
RA 2,527,749 T→C G268G (GGT→GGC yfeH → putative solute:Na(+) symporter
RA 2,560,020 T→C I388T (ATT→ACT)  intZ → CPZ‑55 prophage; putative phage integrase IntZ
RA 2,565,832 (C)5→6 coding (1053/1404 nt) eutA ← reactivating factor for ethanolamine ammonia‑lyase EutBC
RA 2,589,059 A→G T489A (ACT→GCT)  acrD → multidrug efflux pump RND permease AcrD
RA 2,665,067 T→C A226A (GCT→GCC yfhR → putative peptidase
RA 2,667,370 G→A I159I (ATC→ATT hcaT ← putative 3‑phenylpropionate transporter
RA 2,703,772 G→A P98S (CCA→TCA)  rnc ← RNase III
RA 2,749,014 G→A G81S (GGC→AGC)  yfjD → putative inner membrane protein
RA 2,765,694 G→A A28V (GCA→GTA)  yfjM ← CP4‑57 prophage; protein YfjM
RA 2,777,543 G→A E31K (GAA→AAA)  ypjF → CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system
RA 2,809,113 G→A pseudogene (798/1178 nt) ygaY → putative transporter YgaY, N‑terminal fragment
RA 2,833,866 G→A C464Y (TGC→TAC)  norV → anaerobic nitric oxide reductase flavorubredoxin
RA 2,876,347 A→G intergenic (‑18/‑234) cysD ← / → iap sulfate adenylyltransferase subunit 2/alkaline phosphatase isozyme conversion protein
RA 2,877,743 G→A intergenic (+125/+826) iap → / ← cas2 alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
RA 2,900,470 A→G intergenic (‑197/‑122) ygcW ← / → yqcE putative deoxygluconate dehydrogenase/putative transport protein YqcE
RA 2,903,704 C→T intergenic (+330/+1043) ygcE → / ← queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
RA 2,935,179 T→C G315G (GGT→GGC fucP → L‑fucose:H(+) symporter
RA 2,974,350 G→A R555R (CGC→CGT aas ← acyltransferase
RA 2,977,660 A→C L414V (TTA→GTA)  lysA ← diaminopimelate decarboxylase
RA 2,990,649 T→C pseudogene (96/490 nt) ygeF → protein YgeF
RA 3,009,831 C→T G356G (GGC→GGT ygeY → putative peptidase YgeY
RA 3,017,921 G→A C621Y (TGC→TAC)  ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,020,004 T→C F282L (TTC→CTC)  ssnA → putative aminohydrolase
RA 3,020,184 G→A A342T (GCC→ACC)  ssnA → putative aminohydrolase
RA 3,038,422 A→G L134P (CTG→CCG)  dsbC ← protein disulfide isomerase DsbC
RA 3,068,404 G→A S135S (AGC→AGT argO ← L‑arginine exporter
RA 3,080,655 G→A R327R (CGC→CGT tktA ← transketolase 1
RA 3,161,930 T→C N247S (AAC→AGC)  ftsP ← cell division protein required during stress conditions
RA 3,181,406 G→A G329D (GGC→GAC)  ygiC → putative enzyme with ATPase activity
RA 3,245,135 (T)8→9 intergenic (‑394/‑89) uxaC ← / → exuT D‑glucoronate/D‑galacturonate isomerase/hexuronate transporter
RA 3,250,546 C→T intergenic (+170/‑16) yqjK → / → yqjF conserved protein YqjK/DoxX family protein
RA 3,274,540 C→T G123D (GGC→GAC)  garP ← galactarate/glucarate/glycerate transporter GarP
RA 3,310,748 A→G S141P (TCT→CCT)  pnp ← polynucleotide phosphorylase
RA 3,339,507 T→C I187V (ATT→GTT)  mlaF ← intermembrane phospholipid transport system, ATP binding subunit MlaF
RA 3,341,494 C→A P77T (CCT→ACT)  kdsD → D‑arabinose 5‑phosphate isomerase KdsD
RA 3,372,795 T→C Y261C (TAT→TGT)  nanA ← N‑acetylneuraminate lyase
RA 3,405,323 T→C intergenic (+865/‑113) yhdH → / → accB acrylyl‑CoA reductase/biotin carboxyl carrier protein
RA 3,432,176 C→A V97L (GTG→TTG)  smg ← DUF494 domain‑containing protein Smg
RA 3,484,088 (C)6→7 coding (887/1023 nt) yheT → putative hydrolase YheT
RA 3,492,250 A→G S16S (TCT→TCC ppiA ← peptidyl‑prolyl cis‑trans isomerase A
RA 3,494,457 C→T A149A (GCC→GCT nirB → nitrite reductase catalytic subunit NirB
RA 3,521,219 T→C P77P (CCA→CCG hofO ← DNA utilization protein HofO
RA 3,530,370 A→G E199E (GAA→GAG hslO → molecular chaperone Hsp33
RA 3,584,362 A→G intergenic (+53/‑42) yrhA → / → yrhD putative uncharacterized protein YrhA/uncharacterized protein YrhD
RA 3,596,545 C→T G339E (GGG→GAG)  livK ← L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein
RA 3,607,955 T→C V502A (GTC→GCC)  zntA → Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
RA 3,656,479 T→C T88A (ACT→GCT)  hdeA ← HdeA monomer, chaperone active form
RA 3,664,111 (T)8→7 coding (508/729 nt) gadW ← DNA‑binding transcriptional dual regulator GadW
RA 3,694,890 T→C Y116C (TAC→TGC)  bcsA ← cellulose synthase catalytic subunit
RA 3,698,595 G→A A128T (GCC→ACC)  bcsG → cellulose phosphoethanolamine transferase
RA 3,733,248 G→A G350S (GGC→AGC)  xylG → xylose ABC transporter ATP binding subunit
RA 3,759,734 G→A A43V (GCC→GTC)  selB ← selenocysteyl‑tRNA‑specific translation elongation factor
RA 3,794,218 T→C C78R (TGC→CGC)  rfaD → ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase
RA 3,799,022 T→C E97E (GAA→GAG waaU ← putative ADP‑heptose:LPS heptosyltransferase 4
RA 3,879,747 C→T A125T (GCC→ACC)  gyrB ← DNA gyrase subunit B
RA 3,917,420 (C)5→6 coding (846/864 nt) atpG ← ATP synthase F1 complex subunit gamma
RA 3,999,034 C→T A351V (GCC→GTC)  uvrD → ssDNA translocase and dsDNA helicase ‑ DNA helicase II
RA 4,082,606 A→G W52R (TGG→CGG)  fdoH ← formate dehydrogenase O subunit beta
RA 4,133,750 C→T intergenic (+244/‑85) metF → / → katG 5,10‑methylenetetrahydrofolate reductase/hydroperoxidase I
RA 4,158,512 A→G Y8C (TAC→TGC)  oxyR → DNA‑binding transcriptional dual regulator OxyR
RA 4,178,981 A→G T35A (ACT→GCT)  rplA → 50S ribosomal subunit protein L1
RA 4,179,794 C→T intergenic (+211/‑202) rplA → / → rplJ 50S ribosomal subunit protein L1/50S ribosomal subunit protein L10
RA 4,240,162 A→G intergenic (‑107/‑163) yjbT ← / → psiE PF17089 family protein YjbT/putative phosphate starvation‑inducible protein
RA 4,242,488 A→G intergenic (‑234/+138) xylE ← / ← malG D‑xylose:H(+) symporter/maltose ABC transporter membrane subunit MalG
RA 4,262,865 G→A G9D (GGC→GAC)  pspG → phage shock protein G
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,329,894 (G)6→7 coding (535/879 nt) yjcZ → uncharacterized protein YjcZ
RA 4,340,409 Δ1 bp coding (113/2268 nt) adiA ← arginine decarboxylase, degradative
RA 4,392,261 (A)8→9 intergenic (+112/‑99) orn → / → glyV oligoribonuclease/tRNA‑Gly
RA 4,393,081 A→G S329S (AGT→AGC queG ← epoxyqueuosine reductase
RA 4,410,312 T→C S60S (AGT→AGC yjfI → conserved protein YjfI
RA 4,416,734 A→G F13L (TTT→CTT)  bsmA ← DUF1471 domain‑containing putative lipoprotein BsmA
RA 4,424,457 (A)8→9 intergenic (+71/+59) ulaF → / ← yjfY L‑ribulose‑5‑phosphate 4‑epimerase UlaF/DUF1471 domain‑containing protein YjfY
RA 4,440,749 C→T G156D (GGC→GAC)  ytfL ← putative inner membrane protein
RA 4,443,868 C→T A496V (GCC→GTC)  tamA → translocation and assembly module subunit TamA
RA 4,468,399 G→A G259S (GGT→AGT)  mgtA → Mg(2(+)) importing P‑type ATPase
RA 4,492,583 (G)6→7 intergenic (‑58/+4) idnT ← / ← idnO L‑idonate/5‑ketogluconate/gluconate transporter/5‑keto‑D‑gluconate 5‑reductase
RA 4,509,873 A→G pseudogene (70/330 nt) yjhV → KpLE2 phage‑like element; putative uncharacterized protein YjhV
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,627,705 T→C F131L (TTT→CTT)  nadR → DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
RA 4,633,405 A→G V114A (GTG→GCG)  yjjX ← ITPase/XTPase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 339828–339934 340046–339980 47–219 162 [157] [157] 163 yahH putative uncharacterized protein YahH
* * ÷ NC_000913 361201 361928 728 159 [155] [158] 159 lacA–[lacY] lacA,[lacY]
* * ÷ NC_000913 2856977 2857095 119 160 [158] [0] 275 [mutS] [mutS]
* * ÷ NC_000913 3423691–3424234 3424613–3424238 5–923 161 [154] [157] 160 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 70046 =239 (0.930)259 (1.030) 140/188 0.1 69.1% coding (3/1701 nt) araB ribulokinase
?NC_000913 2857096 = 0 (0.000)coding (4/2562 nt) mutS DNA mismatch repair protein MutS