breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 44,865 T→A 100% intergenic (‑46/‑455) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
RA 44,870 2 bp→TC 100% intergenic (‑51/‑449) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
RA 132,185 G→A 100% D560N (GAC→AAC)  fusA → elongation factor G
RA 177,101 G→T 9.1% M1F (TTG→TTT) † EO946_RS01025 → SulP family inorganic anion transporter
RA 240,198 G→T 14.9% Q112K (CAA→AAA)  EO946_RS01360 ← hypothetical protein
RA 240,207 G→T 10.5% Q109K (CAA→AAA)  EO946_RS01360 ← hypothetical protein
RA 240,218 C→A 5.6% G105V (GGA→GTA)  EO946_RS01360 ← hypothetical protein
RA 293,858 A→C 7.4% N79T (AAT→ACT)  EO946_RS01645 → aldo/keto reductase
RA 316,300 G→C 8.9% L40L (CTG→CTC opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA
RA 607,410 C→G 5.7% P85A (CCA→GCA)  EO946_RS03185 → collagen‑like protein
JC 607,424 27 bp→108 bp 44.4% coding (267‑293/1125 nt) EO946_RS03185 → collagen‑like protein
RA 607,700 A→C 12.2% E181D (GAA→GAC EO946_RS03185 → collagen‑like protein
RA 607,838 A→G 8.6% T227T (ACA→ACG EO946_RS03185 → collagen‑like protein
RA 634,052 T→G 17.4% E573D (GAA→GAC EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein
RA 648,130 A→T 7.0% L435L (CTA→CTT EO946_RS03395 → MFS transporter
RA 755,531 A→C 10.5% Q22H (CAA→CAC EO946_RS03880 → rhamnogalacturonan acetylesterase
RA 892,198 G→A 5.4% P283P (CCG→CCA EO946_RS04510 → iron ABC transporter permease
RA 1,170,453 A→C 5.5% K312N (AAA→AAC EO946_RS06025 → carbamoyl phosphate synthase large subunit
RA 1,215,179 A→C 5.7% K81N (AAA→AAC thiF → thiazole biosynthesis adenylyltransferase ThiF
RA 1,243,010 T→A 5.0% V650E (GTG→GAG)  manP → PTS mannose transporter subunit IIABC
RA 1,243,026 A→T 5.6% intergenic (+12/‑4) manP → / → manA PTS mannose transporter subunit IIABC/mannose‑6‑phosphate isomerase, class I
RA 1,263,998 C→A 11.0% L62F (TTG→TTT fetB ← iron export ABC transporter permease subunit FetB
RA 1,488,182 G→T 6.8% A575S (GCA→TCA)  ade → adenine deaminase
RA 1,720,712 C→A 14.3% T212T (ACC→ACA spoIIIE → DNA translocase SpoIIIE
RA 1,753,584 G→T 12.6% intergenic (+275/‑230) fabD → / → EO946_RS09090 ACP S‑malonyltransferase/acyltransferase domain‑containing protein
RA 1,753,622 C→A 7.0% intergenic (+313/‑192) fabD → / → EO946_RS09090 ACP S‑malonyltransferase/acyltransferase domain‑containing protein
RA 2,098,642 C→A 6.5% V297F (GTC→TTC)  EO946_RS10535 ← solute:sodium symporter family transporter
RA 2,179,638 G→T 7.2% L212F (TTG→TTT EO946_RS11020 → queuosine precursor transporter
RA 2,214,528 G→T 6.2% Q57K (CAA→AAA)  nth ← endonuclease III
RA 2,502,212 T→G 11.7% N284H (AAC→CAC)  hrcA ← heat‑inducible transcriptional repressor HrcA
RA 2,900,793 C→A 6.5% P118P (CCC→CCA EO946_RS14945 → extracellular solute‑binding protein
RA 2,979,248 T→C 27.6% noncoding (1553/2928 nt) EO946_RS15430 ← 23S ribosomal RNA
RA 2,980,160 G→A 15.6% noncoding (641/2928 nt) EO946_RS15430 ← 23S ribosomal RNA
RA 3,052,465 G→A 6.1% T111T (ACG→ACA EO946_RS15780 → Na+/H+ antiporter subunit E
RA 3,169,618 G→A 5.3% I63I (ATC→ATT EO946_RS16390 ← carboxymuconolactone decarboxylase family protein
RA 3,169,675 T→C 5.3% Q44Q (CAA→CAG EO946_RS16390 ← carboxymuconolactone decarboxylase family protein
RA 3,219,734 C→A 7.1% Q358K (CAA→AAA)  oxdC → oxalate decarboxylase
RA 3,247,065 C→T 100% intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
RA 3,285,491 T→C 5.5% I304V (ATT→GTT)  EO946_RS16995 ← IS3 family transposase
RA 3,285,567 C→T 32.2% L278L (CTG→CTA EO946_RS16995 ← IS3 family transposase
RA 3,285,575 T→G 32.3% N276H (AAT→CAT)  EO946_RS16995 ← IS3 family transposase
RA 3,285,580 A→G 36.0% I274T (ATA→ACA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,647 C→A 37.9% V252L (GTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,713 G→A 28.6% L230L (CTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,446,995 C→A 6.3% V197V (GTG→GTT degU ← two‑component system response regulator DegU
RA 3,447,038 T→G 9.8% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU
RA 3,562,015 A→C 11.2% intergenic (‑661/‑137) EO946_RS18300 ← / → EO946_RS21105 response regulator/HAMP domain‑containing histidine kinase
RA 3,791,922 T→G 5.7% E126D (GAA→GAC EO946_RS19535 ← DNA‑3‑methyladenine glycosylase
RA 3,843,776 C→G 7.4% V161L (GTT→CTT)  EO946_RS19775 ← hypothetical protein
RA 3,843,812 C→A 9.9% E149* (GAG→TAG)  EO946_RS19775 ← hypothetical protein
RA 3,851,230 G→T 5.7% Q49K (CAG→AAG)  EO946_RS19820 ← hypothetical protein
RA 3,851,237 G→T 6.9% I46I (ATC→ATA EO946_RS19820 ← hypothetical protein
RA 3,906,067 A→C 12.0% I42S (ATT→AGT)  EO946_RS20100 ← DUF3255 family protein
RA 3,996,926 T→A 7.2% N132Y (AAT→TAT)  EO946_RS20530 ← VOC family protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 91969NA (NA)5 (0.020) 5/250 NT NA intergenic (+131/‑39) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
?NZ_CP034943 91973 = NA (NA)intergenic (+135/‑35) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA