breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 5,439 A→C 12.8% H69P (CAC→CCC)  Y75_RS00030 → DUF2502 domain‑containing protein
RA 12,484 C→A 15.9% Q108K (CAG→AAG)  dnaK → molecular chaperone DnaK
RA 46,798 A→G 9.5% D331G (GAT→GGT)  Y75_RS00235 → MFS transporter
RA 117,417 T→G 12.0% T45P (ACC→CCC)  ppdD ← prepilin peptidase‑dependent pilin
RA 131,380 T→G 13.8% intergenic (‑120/‑235) Y75_RS00595 ← / → acnB DUF3300 domain‑containing protein/bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA 220,098 G→A 10.4% intergenic (‑103/+15) rcsF ← / ← metQ Rcs stress response system protein RcsF/methionine ABC transporter substrate‑binding lipoprotein MetQ
RA 249,538 T→G 30.4% H187P (CAC→CCC)  flhA ← flagellar type III secretion system protein FlhA
RA 432,873 T→G 25.5% G65G (GGT→GGG ribD → bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase RibD
RA 456,415 G→T 14.0% G172V (GGT→GTT)  clpP → ATP‑dependent Clp endopeptidase proteolytic subunit ClpP
RA 547,694 A→G 100% E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,831:1 +G 100% coding (251/261 nt) Y75_RS02655 → hypothetical protein
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
RA 623,178 G→T 13.8% Q186K (CAA→AAA)  fepB ← Fe2+‑enterobactin ABC transporter substrate‑binding protein
MC JC 654,214 Δ1,199 bp 100% Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
JC 694,571 Δ88 bp 85.4% coding (720‑807/1425 nt) miaB ← tRNA (N6‑isopentenyl adenosine(37)‑C2)‑methylthiotransferase MiaB
RA 776,480 T→G 9.0% G70G (GGT→GGG tolR → colicin uptake protein TolR
RA 802,540 A→C 15.5% T78P (ACC→CCC)  Y75_RS04020 → anion permease
RA 954,796 C→G 9.6% P31P (CCG→CCC focA ← formate transporter FocA
RA 954,817 G→A 11.6% V24V (GTC→GTT focA ← formate transporter FocA
RA 1,046,494 A→C 11.5% V625G (GTA→GGA)  Y75_RS05125 ← YjbH domain‑containing protein
RA 1,063,614 A→C 13.8% V195G (GTG→GGG)  cbpA ← curved DNA‑binding protein
RA 1,065,700 C→T 100% R415C (CGT→TGT)  Y75_RS05220 → YccE family protein
RA 1,093,686 T→C 100% V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
RA 1,239,439 A→C 10.3% T98P (ACC→CCC)  Y75_RS06220 → D‑amino acid dehydrogenase
RA 1,249,435 A→C 9.7% G419G (GGT→GGG dhaM ← dihydroxyacetone kinase subunit DhaM
RA 1,288,141 T→G 9.9% intergenic (+38/+146) narI → / ← Y75_RS06440 respiratory nitrate reductase subunit gamma/RtT sRNA
RA 1,303,712 A→G 100% S273G (AGC→GGC)  oppA → oligopeptide ABC transporter substrate‑binding protein OppA
RA 1,327,694 A→T 17.8% Y412F (TAC→TTC)  Y75_RS06660 → DUF2207 domain‑containing protein
RA 1,327,721 C→G 7.9% A421G (GCA→GGA)  Y75_RS06660 → DUF2207 domain‑containing protein
RA 1,378,854 A→C 17.9% K103N (AAA→AAC Y75_RS06935 → Gfo/Idh/MocA family oxidoreductase
RA 1,385,432 C→A 12.2% E82* (GAA→TAA)  Y75_RS06960 ← LacI family transcriptional regulator
MC JC 1,413,639 Δ23,060 bp 100% [ttcA]Y75_RS24865 33 genes
RA 1,521,793 C→G 10.7% intergenic (+15/‑183) curA → / → Y75_RS07665 NADPH‑dependent curcumin/dihydrocurcumin reductase/DNA‑binding transcriptional regulator McbR
RA 1,555,745 C→G 14.9% A547P (GCC→CCC)  Y75_RS07805 ← malate dehydrogenase
RA 1,719,676 C→T 9.4% E16K (GAA→AAA)  pdxH ← pyridoxamine 5'‑phosphate oxidase
RA 1,739,298 A→G 100% intergenic (‑39/‑260) ynhF ← / → purR stress response protein YnhF/HTH‑type transcriptional repressor PurR
RA 2,016,354 C→A 16.1% A330E (GCA→GAA)  fliF → flagellar basal body M‑ring protein FliF
RA 2,120,992 A→T 85.3% L435Q (CTG→CAG)  wzxC ← M‑antigen undecaprenyl disphosphate flippase
RA 2,153,826 G→T 24.9% intergenic (+43/+22) Y75_RS10870 → / ← pphC YegJ family protein/protein‑serine/threonine phosphatase PphC
RA 2,171,103 A→C 14.1% I85I (ATA→ATC yegS → lipid kinase YegS
MC JC 2,176,983 Δ1,199 bp 100% Y75_RS10980[gatA] Y75_RS10980, [gatA]
RA 2,208,987 C→T 11.3% A353V (GCT→GTT)  Y75_RS11115 → MoxR family ATPase
MC JC 2,320,795 Δ1,336 bp 100% Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,418,598 T→C 15.5% intergenic (‑20/‑318) Y75_RS12065 ← / → ackA DUF412 domain‑containing protein/acetate kinase
RA 2,583,716 A→G 8.3% S156P (TCC→CCC)  aegA ← formate‑dependent uric acid utilization protein AegA
RA 2,606,887 A→G 21.0% M439V (ATG→GTG)  hyfF → hydrogenase 4 subunit F
RA 2,660,468 T→A 100% H107L (CAC→CTC)  iscR ← Fe‑S cluster assembly transcriptional regulator IscR
RA 2,676,389 T→C 9.0% E210G (GAA→GGA)  Y75_RS13325 ← sugar ABC transporter ATP‑binding protein
RA 2,787,258 C→T 11.0% pseudogene (961/1008 nt) Y75_RS24085 → alpha amylase
RA 2,866,109 C→A 100% pseudogene (99/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 2,877,655 A→T 7.8% S258R (AGT→AGA cas1e ← type I‑E CRISPR‑associated endonuclease Cas1
RA 2,906,975 C→G 100% D450H (GAC→CAC)  pyrG ← CTP synthase (glutamine hydrolyzing)
RA 2,911,891 T→G 100% L139F (TTA→TTC Y75_RS14515 ← GTP pyrophosphokinase
RA 2,939,008 A→C 8.7% C298G (TGC→GGC)  rlmM ← 23S rRNA (cytidine(2498)‑2'‑O)‑methyltransferase RlmM
RA 2,976,822 T→A 11.7% Y245F (TAT→TTT)  lysA ← diaminopimelate decarboxylase
RA 3,063,461 G→A 12.5% E2K (GAA→AAA)  scpC → propionyl‑CoA:succinate CoA transferase
RA 3,205,094 G→A 17.1% intergenic (‑182/‑25) ttdR ← / → ttdA DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
RA 3,205,127 C→G 8.8% S3R (AGC→AGG ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,132 G→A 14.3% S5N (AGT→AAT) ‡ ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,133 T→A 18.7% S5R (AGT→AGA) ‡ ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,139 G→A 14.8% K7K (AAG→AAA ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,140 C→A 16.8% Q8K (CAA→AAA)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,147 C→A 14.5% A10E (GCA→GAA)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,162 C→A 12.4% T15K (ACA→AAA)  ttdA → L(+)‑tartrate dehydratase subunit alpha
MC JC 3,261,134 Δ1,199 bp 100% Y75_RS16205[tdcD] Y75_RS16205, [tdcD]
RA 3,447,067 A→C 9.4% intergenic (‑172/+41) Y75_RS17145 ← / ← rpoC heat‑shock protein C/DNA‑directed RNA polymerase subunit beta'
RA 3,448,400 G→A 15.9% R978C (CGT→TGT)  rpoC ← DNA‑directed RNA polymerase subunit beta'
RA 3,545,595 T→G 25.4% P427P (CCT→CCG Y75_RS17610 → PTS fructose‑like transporter subunit IIBC
RA 3,560,804 T→G 11.5% T109P (ACC→CCC)  yihX ← glucose‑1‑phosphatase
RA 3,609,469 G→C 13.2% A229G (GCG→GGG)  fre ← NAD(P)H‑flavin reductase
RA 3,609,535 G→C 7.5% A207G (GCG→GGG)  fre ← NAD(P)H‑flavin reductase
RA 3,645,110 A→T 100% L140Q (CTG→CAG)  cyaA ← class I adenylate cyclase
RA 3,656,455 C→T 100% V91I (GTT→ATT)  rffM ← lipopolysaccharide N‑acetylmannosaminouronosyltransferase
RA 3,657,819 T→G 12.3% T88P (ACC→CCC)  wzyE ← O‑antigen assembly polymerase
RA 3,741,196 C→G 10.8% R375R (CGC→CGG Y75_RS18545 → adenine permease AdeP
MC JC 3,742,936 Δ1,336 bp 100% Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
MC JC 3,746,911 Δ1,199 bp 100% Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,750,591 Δ1,199 bp 100% Y75_RS18600 Y75_RS18600
RA 3,857,502 T→G 20.4% V250G (GTG→GGG)  gpsA → NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
RA 3,878,107 T→C 26.2% G42G (GGA→GGG Y75_RS19225 ← RHS element protein RhsA
RA 3,942,639 T→C 5.9% D440G (GAT→GGT)  bcsE ← cellulose biosynthesis protein BcsE
RA 4,044,279 A→G 6.1% S90G (AGT→GGT)  livH → high‑affinity branched‑chain amino acid ABC transporter permease LivH
RA 4,119,568 C→A 7.9% Q58K (CAA→AAA)  hofP → DNA utilization protein HofP
RA 4,154,031 G→T 8.5% A89E (GCG→GAG)  crp ← cAMP‑activated global transcriptional regulator CRP
RA 4,245,517 T→A 9.0% T110S (ACC→TCC)  Y75_RS21020 ← D‑xylose transporter XylE
RA 4,371,271 Δ2 bp 100% pseudogene (182‑183/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter
RA 4,446,876 T→A 9.6% intergenic (‑20/‑186) msrA ← / → tamA peptide‑methionine (S)‑S‑oxide reductase MsrA/autotransporter assembly complex protein TamA
RA 4,458,032 A→G 9.0% E260G (GAG→GGG)  Y75_RS22070 → ABC transporter permease
RA 4,479,381 C→A 8.9% T193N (ACT→AAT)  Y75_RS22170 → TetR/AcrR family transcriptional regulator
RA 4,495,938 A→G 24.8% I423T (ATT→ACT)  idnT ← gnt‑II system L‑idonate transporter
RA 4,527,463 T→G 14.3% T438P (ACC→CCC)  Y75_RS22420 ← xylonate dehydratase YjhG
RA 4,611,582 A→G 16.0% S267P (TCC→CCC)  rsmC ← 16S rRNA (guanine(1207)‑N(2))‑methyltransferase RsmC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1301041–1302370 1302407 38–1367 165 [0] [0] 168 Y75_RS06530–Y75_RS06530 Y75_RS06530,Y75_RS06530
* * ÷ NC_007779 1397879–1398957 1406267 7311–8389 53 [51] [0] 130 [Y75_RS07025]–[Y75_RS07065] [Y75_RS07025],Y75_RS07030,uspE,Y75_RS07040,ogt,abgT,Y75_RS07060,[Y75_RS07065]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 273180 =NA (NA)154 (1.000) 85/380 NT 100% intergenic (+3/+145) Y75_RS01330/Y75_RS01335 hypothetical protein/IS5‑like element IS5 family transposase
?NC_007779 701988 = 0 (0.000)coding (29/1221 nt) nagC DNA‑binding transcriptional regulator NagC
* ? NC_007779 = 57358179 (0.510)26 (0.170) 24/382 NT 24.8% intergenic (+19/+379) quuD/Y75_RS02845 antitermination protein QuuD/IS5‑like element IS5 family transposase
?NC_007779 573814 = NA (NA)intergenic (+252/+146) quuD/Y75_RS02845 antitermination protein QuuD/IS5‑like element IS5 family transposase
* ? NC_007779 607256 =NA (NA)10 (0.080) 8/330 NT 6.7% intergenic (+45/‑32) Y75_RS03010/Y75_RS03015 type I toxin‑antitoxin system toxin HokE/IS4‑like element IS421 family transposase
?NC_007779 608549 = 161 (1.040)intergenic (+149/+133) Y75_RS03015/entD IS4‑like element IS421 family transposase/enterobactin synthase subunit EntD
* ? NC_007779 = 689467NA (NA)137 (0.890) 76/382 NT 100% intergenic (‑32/+298) Y75_RS03405/lnt IS5‑like element IS5 family transposase/apolipoprotein N‑acyltransferase
?NC_007779 = 701992 0 (0.000)coding (25/1221 nt) nagC DNA‑binding transcriptional regulator NagC
* ? NC_007779 1106373 =NA (NA)16 (0.110) 5/360 NT 73.9% coding (174/914 nt) Y75_RS05455 IS3‑like element IS2 family transposase
?NC_007779 1106739 = 6 (0.040)coding (168/361 nt) Y75_RS05455 IS3‑like element IS2 family transposase
* ? NC_007779 1209367 =95 (0.620)91 (0.640) 64/350 NT 58.1% coding (290/630 nt) Y75_RS06020 hypothetical protein
?NC_007779 1211196 = 44 (0.310)intergenic (‑6/‑66) Y75_RS06035/pinE tail fiber protein/site‑specific DNA recombinase
* ? NC_007779 = 120938295 (0.620)75 (0.530) 53/350 NT 53.4% coding (305/630 nt) Y75_RS06020 hypothetical protein
?NC_007779 = 1211179 44 (0.310)coding (12/495 nt) Y75_RS06035 tail fiber protein
* ? NC_007779 1294773 =161 (1.040)67 (0.430) 50/382 NT 29.4% intergenic (‑274/‑331) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 = 1398952 NA (NA)intergenic (+146/‑127) Y75_RS07025/Y75_RS07030 IS5‑like element IS5 family transposase/DUF2534 family protein
* ? NC_007779 = 1294776163 (1.060)53 (0.340) 39/382 NT 24.5% intergenic (‑277/‑328) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 1397758 = NA (NA)intergenic (‑122/‑32) ynaI/Y75_RS07025 low conductance mechanosensitive channel YnaI/IS5‑like element IS5 family transposase
* ? NC_007779 = 13010400 (0.000)114 (0.870)
+28 bp
60/326 NT 100% intergenic (+218/‑45) ychE/Y75_RS06530 NAAT family transporter YchE/IS3‑like element IS2 family transposase
?NC_007779 = 4311564 NA (NA)pseudogene (914/921 nt) alsK allose kinase
* ? NC_007779 1302408 =0 (0.000)158 (1.020) 96/382 NT 100% intergenic (+50/‑488) Y75_RS06530/oppA IS3‑like element IS2 family transposase/oligopeptide ABC transporter substrate‑binding protein OppA
?NC_007779 1397758 = NA (NA)intergenic (‑122/‑32) ynaI/Y75_RS07025 low conductance mechanosensitive channel YnaI/IS5‑like element IS5 family transposase
* ? NC_007779 1406268 =0 (0.000)124 (0.800) 78/382 NT 100% coding (12/1311 nt) Y75_RS07065 p‑aminobenzoyl‑glutamate hydrolase subunit AbgA
?NC_007779 1980209 = NA (NA)intergenic (‑298/+146) flhD/Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
* ? NC_007779 1885208 =2 (0.010)157 (1.020) 83/382 NT 98.7% coding (307/1446 nt) Y75_RS09485 BCCT family transporter
?NC_007779 2411458 = NA (NA)intergenic (‑147/+15) lrhA/Y75_RS12035 transcriptional regulator LrhA/IS1‑like element IS1A family transposase
* ? NC_007779 = 18852155 (0.030)158 (1.020) 90/382 NT 96.9% coding (314/1446 nt) Y75_RS09485 BCCT family transporter
?NC_007779 = 2412225 NA (NA)intergenic (‑55/‑782) Y75_RS12035/Y75_RS12040 IS1‑like element IS1A family transposase/alanine transaminase AlaA
* ? NC_007779 1909106 =0 (0.000)180 (1.170) 88/382 NT 100% coding (44/210 nt) cspE transcription antiterminator/RNA stability regulator CspE
?NC_007779 2176983 = NA (NA)intergenic (‑3/‑32) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
* ? NC_007779 = 19091090 (0.000)114 (0.740) 79/382 NT 100% coding (41/210 nt) cspE transcription antiterminator/RNA stability regulator CspE
?NC_007779 1980209 = NA (NA)intergenic (‑298/+146) flhD/Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
* ? NC_007779 2279166 =70 (0.450)6 (0.040) 6/366 NT 7.9% coding (560/1026 nt) Y75_RS11435 ABC transporter permease
?NC_007779 2279202 = 73 (0.490)coding (596/1026 nt) Y75_RS11435 ABC transporter permease
* ? NC_007779 = 2293446NA (NA)11 (0.070) 8/380 NT 6.0% intergenic (‑31/‑388) Y75_RS11495/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
?NC_007779 2477405 = 173 (1.120)coding (104/519 nt) Y75_RS12365 hypothetical protein
* ? NC_007779 3748316 =0 (0.000)168 (1.090) 84/382 NT 100% pseudogene (665/873 nt) tnaB low affinity tryptophan permease TnaB
?NC_007779 = 4056987 NA (NA)coding (391/417 nt) Y75_RS20060 hypothetical protein
* ? NC_007779 = 37483243 (0.020)118 (0.770) 70/382 NT 97.5% pseudogene (657/873 nt) tnaB low affinity tryptophan permease TnaB
?NC_007779 4056220 = NA (NA)intergenic (‑209/+15) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
* ? NC_007779 4056221 =NA (NA)124 (0.810) 82/380 NT 97.6% intergenic (‑210/+14) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
?NC_007779 4370787 = 3 (0.020)pseudogene (667/1304 nt) Y75_RS21600 anaerobic C4‑dicarboxylate transporter
* ? NC_007779 = 4056987NA (NA)182 (1.180) 104/382 NT 97.8% coding (391/417 nt) Y75_RS20060 hypothetical protein
?NC_007779 = 4370795 4 (0.030)pseudogene (659/1304 nt) Y75_RS21600 anaerobic C4‑dicarboxylate transporter
* ? NC_007779 = 43103620 (0.000)207 (1.370) 96/374 NT 100% intergenic (‑286/‑33) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 4311562 = 0 (0.000)pseudogene (916/921 nt) alsK allose kinase