breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 1,843,935 G→A L301F (CTC→TTC)  gacS ← sensor protein GacS
RA 1,851,914 G→T D320Y (GAC→TAC)  relA → ATP:GTP 3'‑pyrophosphotransferase
RA 4,741,273 +C intergenic (+464/+288) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,283 C→G intergenic (+474/+278) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,285 2 bp→CT intergenic (+476/+275) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947 171405–172965 176278–172969 5–4874 2 [1] [1] 2 [PP_16SA]–[PP_5SA] [PP_16SA],PP_23SA,[PP_5SA]
* * ÷ NC_002947 176676–178395 181678–178399 5–5003 2 [1] [0] 2 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947 1131740 1131806 67 2 [1] [0] 2 PP_0992 hypothetical protein
* * ÷ NC_002947 1370992 1371011 20 2 [0] [1] 2 PP_1196 hypothetical protein
* * ÷ NC_002947 2167109 2167154 46 3 [1] [1] 2 PP_1921 hypothetical protein
* * ÷ NC_002947 3109048 3109133 86 2 [1] [1] 2 PP_2722 hypothetical protein
* * ÷ NC_002947 3160600 3160629 30 3 [1] [1] 2 PP_2775 glycine betaine/L‑proline ABC transporter substrate‑binding protein
* * ÷ NC_002947 3445437 3445467 31 2 [1] [1] 2 PP_3064 tail protein
* * ÷ NC_002947 3676183 3676215 33 2 [1] [0] 2 PP_5592 hypothetical protein
* * ÷ NC_002947 3740990 3741029 40 2 [1] [1] 3 [PP_3305] [PP_3305]
* * ÷ NC_002947 5414289 5414405 117 2 [1] [1] 3 PP_4755/gabP‑V ferrichrome‑iron receptor/gamma‑aminobutyrate permease

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 414338 =13 (0.760)3 (0.180) 3/268 1.9 20.3% coding (153/1050 nt) waaF ADP‑heptose:LPS heptosyltransferase II
?NC_002947 414374 = 11 (0.660)coding (189/1050 nt) waaF ADP‑heptose:LPS heptosyltransferase II
* ? NC_002947 796307 =17 (0.990)4 (0.260) 4/254 1.5 22.5% intergenic (+7/‑156) PP_0680/PP_0681 ATP‑dependent protease‑like protein/hypothetical protein
?NC_002947 796350 = 12 (0.770)intergenic (+50/‑113) PP_0680/PP_0681 ATP‑dependent protease‑like protein/hypothetical protein
* ? NC_002947 1037774 =21 (1.220)4 (0.280) 3/232 1.7 20.8% intergenic (+16/+100) PP_0896/PP_0897 carbon‑nitrogen hydrolase family protein/class 1 fumarate hydratase
?NC_002947 1037852 = 13 (0.910)intergenic (+94/+22) PP_0896/PP_0897 carbon‑nitrogen hydrolase family protein/class 1 fumarate hydratase
* ? NC_002947 = 111489914 (0.820)5 (0.360) 5/228 1.1 28.2% intergenic (‑88/+91) rlmF/valS ribosomal RNA large subunit methyltransferase F/valine‑‑tRNA ligase
?NC_002947 = 1114925 14 (1.000)intergenic (‑114/+65) rlmF/valS ribosomal RNA large subunit methyltransferase F/valine‑‑tRNA ligase
* ? NC_002947 1436018 =16 (0.930)4 (0.240) 4/266 1.6 22.0% intergenic (‑48/+105) PP_1256/PP_1257 alpha‑ketoglutarate semialdehyde dehydrogenase/1‑pyrroline‑4‑hydroxy‑2‑carboxylate deaminase
?NC_002947 1436109 = 13 (0.790)intergenic (‑139/+14) PP_1256/PP_1257 alpha‑ketoglutarate semialdehyde dehydrogenase/1‑pyrroline‑4‑hydroxy‑2‑carboxylate deaminase
* ? NC_002947 1621292 =13 (0.760)4 (0.250) 4/258 1.6 24.2% coding (164/1392 nt) PP_1421 sensor histidine kinase TctE
?NC_002947 1621329 = 13 (0.820)coding (201/1392 nt) PP_1421 sensor histidine kinase TctE
* ? NC_002947 1731205 =13 (0.760)3 (0.220) 3/222 1.6 28.1% intergenic (‑76/+115) dapE/PP_1526 succinyl‑diaminopimelate desuccinylase/beta‑(1‑3)‑glucosyl transferase
?NC_002947 1731297 = 5 (0.370)intergenic (‑168/+23) dapE/PP_1526 succinyl‑diaminopimelate desuccinylase/beta‑(1‑3)‑glucosyl transferase
* ? NC_002947 1967188 =3 (0.170)7 (0.540) 7/212 0.7 86.0% intergenic (+15/+104) PP_1762/yciK hypothetical protein/putative oxidoreductase
?NC_002947 1967284 = 0 (0.000)intergenic (+111/+8) PP_1762/yciK hypothetical protein/putative oxidoreductase
* ? NC_002947 = 19672203 (0.170)7 (0.540) 6/212 0.9 86.0% intergenic (+47/+72) PP_1762/yciK hypothetical protein/putative oxidoreductase
?NC_002947 = 1967250 0 (0.000)intergenic (+77/+42) PP_1762/yciK hypothetical protein/putative oxidoreductase
* ? NC_002947 = 197078914 (0.820)6 (0.370) 4/266 1.6 30.5% intergenic (+40/‑226) mtnA/PP_mr25 methylthioribose‑1‑phosphate isomerase/gyrA regulatory region
?NC_002947 = 1970837 14 (0.850)intergenic (+88/‑178) mtnA/PP_mr25 methylthioribose‑1‑phosphate isomerase/gyrA regulatory region
* ? NC_002947 = 204885312 (0.700)4 (0.270) 4/244 1.5 21.9% intergenic (+49/+90) PP_1821/PP_1822 glutathione S‑transferase family protein/hypothetical protein
?NC_002947 = 2048910 18 (1.200)intergenic (+106/+33) PP_1821/PP_1822 glutathione S‑transferase family protein/hypothetical protein
* ? NC_002947 = 208556212 (0.700)6 (0.370) 4/262 1.6 42.4% intergenic (+228/‑367) PP_1864/PP_1865 hypothetical protein/transposase
?NC_002947 = 2087716 5 (0.310)intergenic (+486/+432) PP_1865/aroH transposase/3‑deoxy‑7‑phosphoheptulonate synthase
* ? NC_002947 2301496 =0 (0.000)17 (1.010) 12/272 0.5 100% intergenic (‑97/‑234) sbcD/PP_5497 exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein
?NC_002947 2925472 = NA (NA)intergenic (‑177/+388) PP_2563/PP_2564 antibiotic biosynthesis protein/quercetin 2,3‑dioxygenase
* ? NC_002947 = 23014970 (0.000)13 (0.770) 13/274 0.4 100% intergenic (‑98/‑233) sbcD/PP_5497 exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein
?NC_002947 5545369 = NA (NA)intergenic (‑461/+416) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
* ? NC_002947 2489400 =10 (0.580)4 (0.240) 4/268 1.6 28.9% coding (354/1578 nt) PP_2184 formate dehydrogenase subunit beta
?NC_002947 2489439 = 10 (0.600)coding (393/1578 nt) PP_2184 formate dehydrogenase subunit beta
* ? NC_002947 2574385 =15 (0.870)5 (0.300) 4/266 1.6 26.1% coding (1098/1566 nt) PP_2257 aerotaxis receptor
?NC_002947 2574435 = 14 (0.850)coding (1148/1566 nt) PP_2257 aerotaxis receptor
* ? NC_002947 = 310892012 (0.700)3 (0.180) 3/266 1.9 21.1% coding (278/465 nt) PP_2722 hypothetical protein
?NC_002947 = 3108938 11 (0.670)coding (260/465 nt) PP_2722 hypothetical protein
* ? NC_002947 3387807 =16 (0.930)5 (0.310) 5/262 1.3 23.2% coding (545/795 nt) PP_2989 short‑chain dehydrogenase/reductase family oxidoreductase
?NC_002947 3387849 = 18 (1.110)coding (587/795 nt) PP_2989 short‑chain dehydrogenase/reductase family oxidoreductase
* ? NC_002947 = 352281312 (0.700)4 (0.240) 4/272 1.6 32.3% intergenic (+557/‑140) PP_5584/PP_3113 hypothetical protein/transposase
?NC_002947 = 3522862 5 (0.300)intergenic (+606/‑91) PP_5584/PP_3113 hypothetical protein/transposase
* ? NC_002947 3974772 =10 (0.580)3 (0.180) 3/268 1.9 23.4% intergenic (‑256/‑85) PP_3503/PP_3504 sigma‑54 dependent transcriptional regulator/hypothetical protein
?NC_002947 3974818 = 10 (0.600)intergenic (‑302/‑39) PP_3503/PP_3504 sigma‑54 dependent transcriptional regulator/hypothetical protein
* ? NC_002947 4586063 =18 (1.050)3 (0.240) 3/202 1.5 26.0% intergenic (+102/+115) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
?NC_002947 4586167 = 4 (0.320)intergenic (+206/+11) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
* ? NC_002947 = 48082243 (0.170)6 (0.410) 4/238 1.4 39.3% intergenic (‑288/‑267) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947 4945248 = 16 (1.090)intergenic (+563/+235) PP_5673/flhB D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein
* ? NC_002947 4893598 =9 (0.520)4 (0.290) 4/224 1.3 31.7% intergenic (+28/‑180) pyk/PP_4302 pyruvate kinase/urea transporter
?NC_002947 4893677 = 10 (0.720)intergenic (+107/‑101) pyk/PP_4302 pyruvate kinase/urea transporter
* ? NC_002947 = 48936248 (0.470)3 (0.220) 3/224 1.6 26.7% intergenic (+54/‑154) pyk/PP_4302 pyruvate kinase/urea transporter
?NC_002947 = 4893649 10 (0.720)intergenic (+79/‑129) pyk/PP_4302 pyruvate kinase/urea transporter
* ? NC_002947 = 489644316 (0.930)9 (0.620) 6/236 1.0 40.4% intergenic (+31/‑177) PP_4304/sbp‑I VIC family cation transporter/sulfate ABC transporter substrate‑binding protein
?NC_002947 = 4896490 13 (0.890)intergenic (+78/‑130) PP_4304/sbp‑I VIC family cation transporter/sulfate ABC transporter substrate‑binding protein
* ? NC_002947 = 50332782 (0.120)5 (0.300) 5/266 1.4 37.2% intergenic (+395/‑15) PP_4435/PP_4437 hypothetical protein/transposase
?NC_002947 6155309 = 15 (0.910)intergenic (‑63/+143) PP_5398/PP_5399 transposase/hypothetical protein
* ? NC_002947 = 51903949 (0.520)3 (0.190) 3/258 1.8 28.1% intergenic (‑81/+93) PP_4570/cysK hypothetical protein/cysteine synthase A
?NC_002947 = 5190411 7 (0.440)intergenic (‑98/+76) PP_4570/cysK hypothetical protein/cysteine synthase A
* ? NC_002947 = 52016638 (0.470)6 (0.480) 6/204 0.8 52.5% intergenic (‑101/‑79) PP_4582/PP_4583 membrane protein/putative Peptidase
?NC_002947 = 5201684 5 (0.400)intergenic (‑122/‑58) PP_4582/PP_4583 membrane protein/putative Peptidase
* ? NC_002947 5288442 =11 (0.640)4 (0.300) 4/218 1.3 37.0% intergenic (‑75/+109) PP_4660/mdaB LysR family transcriptional regulator/drug activity modulator B
?NC_002947 5288536 = 5 (0.370)intergenic (‑169/+15) PP_4660/mdaB LysR family transcriptional regulator/drug activity modulator B
* ? NC_002947 = 567168914 (0.820)7 (0.490) 4/230 1.4 35.4% intergenic (+57/‑113) PP_4978/PP_4979 hypothetical protein/substrate‑binding protein
?NC_002947 = 5671726 14 (0.990)intergenic (+94/‑76) PP_4978/PP_4979 hypothetical protein/substrate‑binding protein
* ? NC_002947 5750393 =15 (0.870)4 (0.240) 4/268 1.6 20.8% coding (1226/1455 nt) thiI tRNA sulfurtransferase
?NC_002947 5750415 = 16 (0.970)coding (1204/1455 nt) thiI tRNA sulfurtransferase
* ? NC_002947 5914213 =20 (1.160)5 (0.370) 5/222 1.1 28.6% intergenic (+49/+104) spuI/argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase
?NC_002947 5914309 = 9 (0.660)intergenic (+145/+8) spuI/argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase
* ? NC_002947 6027949 =23 (1.340)7 (0.480) 6/236 1.0 30.1% intergenic (‑37/+74) PP_5277/kauB MFS transporter/4‑guanidinobutyraldehyde dehydrogenase
?NC_002947 6028006 = 13 (0.890)intergenic (‑94/+17) PP_5277/kauB MFS transporter/4‑guanidinobutyraldehyde dehydrogenase