breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,756,796 G→T E367* (GAA→TAA)  pykF → pyruvate kinase I
RA 1,757,387 C→G intergenic (+277/‑34) pykF → / → lpp pyruvate kinase I/murein lipoprotein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,312,590 G→A Q54* (CAG→TAG)  ompC ← outer membrane porin protein C
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 3,413,571 T→C intergenic (‑106/‑293) acrS ← / → acrE acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,114,419 G→A A18A (GCC→GCT fpr ← ferredoxin‑NADP reductase; flavodoxin reductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423709–3424234 3424591–3424238 5–883 6 [3] [3] 4 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 15989 =NA (NA)5 (0.220) 4/116 1.9 NA noncoding (603/1345 nt) IS186 repeat region
?NC_000913 16348 = NA (NA)noncoding (962/1345 nt) IS186 repeat region
* ? NC_000913 315401 =NA (NA)6 (0.290) 6/106 1.2 NA noncoding (173/1255 nt) IS3 repeat region
?NC_000913 315627 = NA (NA)noncoding (399/1255 nt) IS3 repeat region
* ? NC_000913 315644 =NA (NA)19 (0.840) 6/116 1.3 NA noncoding (416/1255 nt) IS3 repeat region
?NC_000913 315712 = NA (NA)noncoding (484/1255 nt) IS3 repeat region
* ? NC_000913 = 382141NA (NA)5 (0.200) 3/128 2.4 NA noncoding (882/1331 nt) IS2 repeat region
?NC_000913 = 382258 NA (NA)noncoding (999/1331 nt) IS2 repeat region
* ? NC_000913 = 43815218 (0.700)4 (0.190) 4/106 1.7 22.0% intergenic (‑17/+163) yajO/dxs 2‑carboxybenzaldehyde reductase/1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
?NC_000913 = 438293 14 (0.680)intergenic (‑158/+22) yajO/dxs 2‑carboxybenzaldehyde reductase/1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
* ? NC_000913 1015208 =22 (0.850)7 (0.330) 3/110 2.1 24.4% coding (313/564 nt) pqiC DUF330 family putative lipoprotein
?NC_000913 1015844 = 25 (1.160)coding (130/168 nt) rmf ribosome modulation factor
* ? NC_000913 1207790 =2 (0.080)17 (0.870) 11/100 0.4 90.6% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 2 (0.100)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078052 (0.080)12 (0.620) 8/100 0.7 87.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 2 (0.100)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1307027 =7 (0.270)3 (0.130) 3/114 2.2 22.2% coding (124/330 nt) yciU UPF0263 family protein
?NC_000913 1307124 = 15 (0.670)coding (27/330 nt) yciU UPF0263 family protein
* ? NC_000913 1341858 =14 (0.540)5 (0.210) 4/124 2.0 24.9% intergenic (+131/‑63) yciM/pyrF LPS regulatory protein; putative modulator of LpxC proteolysis/orotidine‑5'‑phosphate decarboxylase
?NC_000913 1341945 = 17 (0.700)coding (25/738 nt) pyrF orotidine‑5'‑phosphate decarboxylase
* ? NC_000913 = 138709820 (0.770)7 (0.300) 3/120 2.3 33.2% coding (379/1542 nt) tyrR aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase
?NC_000913 = 1387248 10 (0.430)coding (529/1542 nt) tyrR aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase
* ? NC_000913 1466676 =14 (0.540)6 (0.260) 3/116 2.2 22.1% pseudogene (1285/2513 nt) ydbA pseudogene, autotransporter homolog; interrupted by IS2 and IS30
?NC_000913 1466937 = 30 (1.320)pseudogene (1546/2513 nt) ydbA pseudogene, autotransporter homolog; interrupted by IS2 and IS30
* ? NC_000913 1633763 =NA (NA)4 (0.170) 3/118 2.2 100% coding (142/591 nt) pinQ Qin prophage; putative site‑specific recombinase
?NC_000913 1633785 = 0 (0.000)coding (164/591 nt) pinQ Qin prophage; putative site‑specific recombinase
* ? NC_000913 1881698 =11 (0.430)3 (0.130) 3/122 2.3 20.6% coding (112/924 nt) dmlR transcriptional activator of dmlA
?NC_000913 1881770 = 13 (0.540)coding (40/924 nt) dmlR transcriptional activator of dmlA
* ? NC_000913 2045242 =16 (0.620)8 (0.380) 4/108 1.7 34.8% coding (305/7077 nt) yeeJ putative adhesin
?NC_000913 2045355 = 17 (0.810)coding (418/7077 nt) yeeJ putative adhesin
* ? NC_000913 = 223656913 (0.500)3 (0.150) 3/102 1.9 21.4% noncoding (36/150 nt) REP156 (repetitive extragenic palindromic) element; contains 3 REP sequences REP156 (repetitive extragenic palindromic) element; contains 3 REP sequences
?NC_000913 = 2236597 12 (0.600)noncoding (64/150 nt) REP156 (repetitive extragenic palindromic) element; contains 3 REP sequences REP156 (repetitive extragenic palindromic) element; contains 3 REP sequences
* ? NC_000913 2632036 =21 (0.810)7 (0.300) 4/118 1.9 33.5% coding (500/1578 nt) guaA GMP synthetase (glutamine aminotransferase)
?NC_000913 2632263 = 9 (0.390)coding (273/1578 nt) guaA GMP synthetase (glutamine aminotransferase)
* ? NC_000913 = 2726279NA (NA)3 (0.130) 3/122 2.3 NA intergenic (‑91/+2) rrfG/rrlG 5S ribosomal RNA of rrnG operon/23S ribosomal RNA of rrnG operon
?NC_000913 = 2726350 NA (NA)noncoding (2835/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
* ? NC_000913 3419891 =39 (1.510)6 (0.300) 3/104 2.0 20.5% pseudogene (785/960 nt) yhdW pseudogene, ABC transporter periplasmic binding protein family;putative transport; Not classified; putative periplasmic binding transport protein; periplasmic‑binding component of ABC superfamily
?NC_000913 3420220 = 16 (0.790)coding (87/1182 nt) yhdX putative amino acid ABC transporter permease
* ? NC_000913 3620066 =NA (NA)3 (0.150) 3/104 2.0 100% coding (875/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3620490 = 0 (0.000)coding (1299/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 379832528 (1.080)14 (0.570) 4/126 2.0 32.7% coding (988/1074 nt) waaU lipopolysaccharide core biosynthesis
?NC_000913 = 3798516 31 (1.260)coding (797/1074 nt) waaU lipopolysaccharide core biosynthesis
* ? NC_000913 = 383104418 (0.700)8 (0.370) 4/110 1.8 33.4% coding (588/1710 nt) yicH putative inner membrane‑anchored periplasmic AsmA family protein
?NC_000913 = 3831092 17 (0.790)coding (636/1710 nt) yicH putative inner membrane‑anchored periplasmic AsmA family protein
* ? NC_000913 = 385488814 (0.540)3 (0.130) 3/114 2.2 21.4% coding (967/1185 nt) emrD multidrug efflux system protein
?NC_000913 = 3854930 10 (0.450)coding (1009/1185 nt) emrD multidrug efflux system protein
* ? NC_000913 3942313 =NA (NA)3 (0.120) 3/126 2.4 NA noncoding (506/1542 nt) rrsC 16S ribosomal RNA of rrnC operon
?NC_000913 3942877 = NA (NA)noncoding (1070/1542 nt) rrsC 16S ribosomal RNA of rrnC operon
* ? NC_000913 4153821 =27 (1.040)5 (0.220) 4/114 1.8 20.3% coding (1027/1152 nt) argE acetylornithine deacetylase
?NC_000913 4154127 = 16 (0.720)coding (721/1152 nt) argE acetylornithine deacetylase
* ? NC_000913 = 454269015 (0.580)7 (0.310) 6/114 1.3 32.6% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 16 (0.720)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)