breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC 1,851,823 (TGCG)2→3 coding (867/2241 nt) relA → ATP:GTP 3'‑pyrophosphotransferase
RA 4,741,273 +C intergenic (+464/+288) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,275 A→G intergenic (+466/+286) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,277 C→T intergenic (+468/+284) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,280 C→T intergenic (+471/+281) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,283 C→G intergenic (+474/+278) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,285 2 bp→CT intergenic (+476/+275) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,965,027 C→A E126* (GAA→TAA)  fleQ ← transcriptional regulator FleQ
RA 5,656,850 C→A G299V (GGT→GTT)  tktA ← transketolase A

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947 171391–173337 173337 1–1947 2 [1] [1] 2 [PP_16SA]–[PP_23SA] [PP_16SA],[PP_23SA]
* * ÷ NC_002947 176681–181683 181683 1–5003 2 [1] [1] 2 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947 2016757 2016800 44 3 [0] [1] 2 PP_1795/PP_1797 hypothetical protein/HlyD family secretion protein
* * ÷ NC_002947 2185558 2185606 49 2 [1] [0] 2 PP_1936 membrane protein
* * ÷ NC_002947 2594791 2594828 38 2 [1] [1] 2 PP_2272 hypothetical protein
* * ÷ NC_002947 3053556 3053603 48 2 [1] [1] 2 agmR glycerol metabolism activator
* * ÷ NC_002947 4808205 4808241 37 10 [0] [1] 2 PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 = 174906NA (NA)3 (0.180) 3/268 2.0 NA noncoding (1704/2902 nt) PP_23SA 23S ribosomal RNA
?NC_002947 = 174936 NA (NA)noncoding (1734/2902 nt) PP_23SA 23S ribosomal RNA
* ? NC_002947 = 31602217 (1.000)5 (0.310) 5/268 1.4 30.9% intergenic (+34/+49) cysQ/PP_0262 3'(2'),5'‑bisphosphate nucleotidase CysQ/hypothetical protein
?NC_002947 336297 = 6 (0.370)intergenic (+142/+251) PP_0278/PP_0279 hypothetical protein/hypothetical protein
* ? NC_002947 = 3580871 (0.060)4 (0.310) 4/212 1.3 82.0% intergenic (+52/‑78) tdcG‑I/PP_5441 L‑serine dehydratase/hypothetical protein
?NC_002947 = 358099 1 (0.080)intergenic (+64/‑66) tdcG‑I/PP_5441 L‑serine dehydratase/hypothetical protein
* ? NC_002947 = 65278524 (1.420)7 (0.480) 6/238 1.0 40.5% coding (1961/1962 nt) ctpL methyl‑accepting chemotaxis protein CtpL
?NC_002947 = 4808204 0 (0.000)intergenic (‑268/‑287) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* ? NC_002947 741473 =NA (NA)4 (0.240) 4/270 1.7 22.7% intergenic (+70/‑430) PP_t12/PP_0635 tRNA‑Thr/group II intron‑encoding maturase
?NC_002947 2069206 = 14 (0.830)intergenic (+10/‑464) PP_t19/PP_1846 tRNA‑Ser/group II intron‑encoding maturase
* ? NC_002947 1037774 =9 (0.530)4 (0.280) 3/232 1.7 37.2% intergenic (+16/+100) PP_0896/PP_0897 carbon‑nitrogen hydrolase family protein/class 1 fumarate hydratase
?NC_002947 1037852 = 6 (0.420)intergenic (+94/+22) PP_0896/PP_0897 carbon‑nitrogen hydrolase family protein/class 1 fumarate hydratase
* ? NC_002947 1296580 =NA (NA)5 (0.320) 5/260 1.4 NA intergenic (‑327/‑239) nhaA‑I/PP_1133 Na(+)/H(+) antiporter NhaA/transposase
?NC_002947 1296616 = NA (NA)intergenic (‑363/‑203) nhaA‑I/PP_1133 Na(+)/H(+) antiporter NhaA/transposase
* ? NC_002947 1306459 =11 (0.650)3 (0.190) 3/260 1.9 21.2% intergenic (‑137/+49) livH/livK branched‑chain amino acid ABC transporter permease/branched‑chain amino acids ABC transporter substrate‑binding protein
?NC_002947 1306483 = 12 (0.760)intergenic (‑161/+25) livH/livK branched‑chain amino acid ABC transporter permease/branched‑chain amino acids ABC transporter substrate‑binding protein
* ? NC_002947 1436017 =14 (0.830)4 (0.240) 3/278 2.0 21.6% intergenic (‑47/+106) PP_1256/PP_1257 alpha‑ketoglutarate semialdehyde dehydrogenase/1‑pyrroline‑4‑hydroxy‑2‑carboxylate deaminase
?NC_002947 1436110 = 15 (0.890)intergenic (‑140/+13) PP_1256/PP_1257 alpha‑ketoglutarate semialdehyde dehydrogenase/1‑pyrroline‑4‑hydroxy‑2‑carboxylate deaminase
* ? NC_002947 1443111 =9 (0.530)4 (0.290) 4/228 1.4 46.0% intergenic (‑42/+134) ghrB/PP_1262 2‑ketoaldonate reductase/hydroxypyruvate/glyoxylate reductase/LysR family transcriptional regulator
?NC_002947 1443179 = 2 (0.140)intergenic (‑110/+66) ghrB/PP_1262 2‑ketoaldonate reductase/hydroxypyruvate/glyoxylate reductase/LysR family transcriptional regulator
* ? NC_002947 1499443 =9 (0.530)4 (0.280) 4/232 1.4 41.0% intergenic (+13/‑153) trpS/zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
?NC_002947 1499526 = 4 (0.280)intergenic (+96/‑70) trpS/zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
* ? NC_002947 1690813 =15 (0.890)4 (0.240) 4/274 1.7 21.8% coding (60/1146 nt) ydcS polyamine ABC transporter, periplasmic polyamine‑binding protein
?NC_002947 1690835 = 14 (0.840)coding (38/1146 nt) ydcS polyamine ABC transporter, periplasmic polyamine‑binding protein
* ? NC_002947 = 17801277 (0.410)3 (0.210) 3/240 1.8 35.2% intergenic (+44/+62) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
?NC_002947 = 1780151 5 (0.340)intergenic (+68/+38) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
* ? NC_002947 1967181 =5 (0.300)4 (0.240) 4/270 1.7 42.4% intergenic (+8/+111) PP_1762/yciK hypothetical protein/putative oxidoreductase
?NC_002947 1967291 = 6 (0.370)intergenic (+118/+1) PP_1762/yciK hypothetical protein/putative oxidoreductase
* ? NC_002947 = 205786614 (0.830)5 (0.360) 5/230 1.2 35.0% intergenic (+39/+89) PP_1832/PP_1833 oxidase/hypothetical protein
?NC_002947 = 2057906 7 (0.500)intergenic (+79/+49) PP_1832/PP_1833 oxidase/hypothetical protein
* ? NC_002947 2241317 =4 (0.240)4 (0.270) 4/244 1.5 59.2% intergenic (+21/‑260) gltX/PP_t25 glutamate‑‑tRNA ligase/tRNA‑Ala
?NC_002947 2241387 = 2 (0.130)intergenic (+91/‑190) gltX/PP_t25 glutamate‑‑tRNA ligase/tRNA‑Ala
* ? NC_002947 = 225384410 (0.590)3 (0.180) 3/270 2.0 25.3% coding (120/1083 nt) leuB 3‑isopropylmalate dehydrogenase
?NC_002947 = 2253880 8 (0.490)coding (156/1083 nt) leuB 3‑isopropylmalate dehydrogenase
* ? NC_002947 = 24721833 (0.180)4 (0.280)
+21 bp
4/236 1.5 22.8% intergenic (+44/+105) PP_2166/PP_2167 anti‑anti‑sigma factor/PhoD‑like phosphatase
?NC_002947 = 5574160 29 (1.710)intergenic (+161/+144) rsgA/motB ribosome biogenesis GTPase RsgA/flagellar motor rotation protein
* ? NC_002947 3947957 =7 (0.410)4 (0.300) 4/222 1.4 45.5% coding (136/1797 nt) PP_3483 type II secretion system protein
?NC_002947 3948042 = 4 (0.300)coding (51/1797 nt) PP_3483 type II secretion system protein
* ? NC_002947 4222784 =12 (0.710)4 (0.240) 4/270 1.7 25.3% coding (115/204 nt) PP_3701 hypothetical protein
?NC_002947 4222804 = 12 (0.730)coding (95/204 nt) PP_3701 hypothetical protein
* ? NC_002947 4348362 =NA (NA)4 (0.250) 4/266 1.6 NA coding (318/1422 nt) PP_3820 group II intron‑encoding maturase
?NC_002947 4392757 = NA (NA)coding (302/1422 nt) PP_3868 group II intron‑encoding maturase
* ? NC_002947 4444087 =13 (0.770)4 (0.240) 4/272 1.7 25.2% intergenic (‑62/+147) nicT/nicF metabolite transport protein NicT/maleamate amidohydrolase
?NC_002947 4444116 = 11 (0.660)intergenic (‑91/+118) nicT/nicF metabolite transport protein NicT/maleamate amidohydrolase
* ? NC_002947 = 489644312 (0.710)4 (0.280) 4/236 1.5 37.8% intergenic (+31/‑177) PP_4304/sbp‑I VIC family cation transporter/sulfate ABC transporter substrate‑binding protein
?NC_002947 = 4896490 3 (0.210)intergenic (+78/‑130) PP_4304/sbp‑I VIC family cation transporter/sulfate ABC transporter substrate‑binding protein
* ? NC_002947 5125135 =8 (0.470)3 (0.220) 3/226 1.7 25.5% intergenic (+61/‑121) PP_4511/PP_4512 AraC family transcriptional regulator/DMT superfamily permease
?NC_002947 5125214 = 11 (0.800)intergenic (+140/‑42) PP_4511/PP_4512 AraC family transcriptional regulator/DMT superfamily permease
* ? NC_002947 5162618 =10 (0.590)6 (0.440) 3/226 1.7 45.9% intergenic (+93/+108) PP_4544/PP_4545 hypothetical protein/beta‑ketodecanoyl‑ACP synthase
?NC_002947 5162702 = 6 (0.440)intergenic (+177/+24) PP_4544/PP_4545 hypothetical protein/beta‑ketodecanoyl‑ACP synthase
* ? NC_002947 = 52016636 (0.350)4 (0.320) 3/204 1.5 41.2% intergenic (‑101/‑79) PP_4582/PP_4583 membrane protein/putative Peptidase
?NC_002947 = 5201684 7 (0.560)intergenic (‑122/‑58) PP_4582/PP_4583 membrane protein/putative Peptidase
* ? NC_002947 5333243 =21 (1.240)6 (0.400) 6/248 1.1 24.6% intergenic (+27/‑216) gluQ/PP_5704 glutamyl‑Q tRNA(Asp) synthetase/hypothetical protein
?NC_002947 5333303 = 18 (1.190)intergenic (+87/‑156) gluQ/PP_5704 glutamyl‑Q tRNA(Asp) synthetase/hypothetical protein
* ? NC_002947 = 55941706 (0.350)3 (0.220) 3/222 1.6 38.0% intergenic (‑66/+79) PP_4921/thiC NCS1 family transporter/phosphomethylpyrimidine synthase
?NC_002947 = 5594207 5 (0.370)intergenic (‑103/+42) PP_4921/thiC NCS1 family transporter/phosphomethylpyrimidine synthase
* ? NC_002947 5652989 =11 (0.650)3 (0.210) 3/236 1.7 28.1% intergenic (‑39/+122) PP_4961/PP_4962 lipoprotein/hypothetical protein
?NC_002947 5653045 = 6 (0.420)intergenic (‑95/+66) PP_4961/PP_4962 lipoprotein/hypothetical protein
* ? NC_002947 5743392 =18 (1.060)4 (0.260) 4/252 1.6 20.9% intergenic (‑169/+78) fbp/glgP class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase
?NC_002947 5743446 = 14 (0.910)intergenic (‑223/+24) fbp/glgP class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase
* ? NC_002947 = 57594919 (0.530)4 (0.310) 4/214 1.3 32.1% intergenic (+44/+112) trmL/secB tRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/protein‑export protein SecB
?NC_002947 = 5759519 10 (0.770)intergenic (+72/+84) trmL/secB tRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/protein‑export protein SecB
* ? NC_002947 5897799 =11 (0.650)4 (0.290) 4/224 1.4 35.0% intergenic (‑143/+91) PP_5172/PP_5173 hypothetical protein/RND family transporter
?NC_002947 5897875 = 6 (0.440)intergenic (‑219/+15) PP_5172/PP_5173 hypothetical protein/RND family transporter
* ? NC_002947 6009165 =4 (0.240)4 (0.310) 6/212 0.9 72.4% intergenic (+6/+76) norM/PP_5263 multidrug resistance protein NorM/GGDEF domain‑containing protein
?NC_002947 6009242 = 0 (0.000)coding (1667/1668 nt) PP_5263 GGDEF domain‑containing protein
* ? NC_002947 6032797 =11 (0.650)4 (0.240) 4/270 1.7 23.8% intergenic (+5/+131) dppA‑IV/PP_5284 dipeptide transport protein/hypothetical protein
?NC_002947 6032901 = 15 (0.910)intergenic (+109/+27) dppA‑IV/PP_5284 dipeptide transport protein/hypothetical protein
* ? NC_002947 = 60605009 (0.530)3 (0.200) 3/250 1.8 21.4% intergenic (+80/+101) PP_5311/PP_5312 signal transduction protein/hypothetical protein
?NC_002947 = 6060559 14 (0.920)intergenic (+139/+42) PP_5311/PP_5312 signal transduction protein/hypothetical protein
* ? NC_002947 = 60977869 (0.530)3 (0.190) 3/258 1.9 22.8% coding (844/963 nt) PP_5348 LysR family transcriptional regulator
?NC_002947 = 6097801 12 (0.760)coding (859/963 nt) PP_5348 LysR family transcriptional regulator