breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 52,254 T→C 5.7% intergenic (+15/‑196) prtG → / → EO946_RS00280 sporulation‑specific protease PrtG/Veg family protein
RA 52,263 T→G 5.7% intergenic (+24/‑187) prtG → / → EO946_RS00280 sporulation‑specific protease PrtG/Veg family protein
RA 112,591 A→T 9.2% intergenic (+269/‑35) gltX → / → cysE glutamate‑‑tRNA ligase/serine O‑acetyltransferase
RA 177,053 C→A 23.2% intergenic (+226/‑46) EO946_RS01020 → / → EO946_RS01025 alcohol dehydrogenase/SulP family inorganic anion transporter
RA 177,101 G→T 6.5% M1F (TTG→TTT) † EO946_RS01025 → SulP family inorganic anion transporter
RA 240,174 T→G 8.7% N120H (AAC→CAC)  EO946_RS01360 ← hypothetical protein
RA 240,207 G→T 12.9% Q109K (CAA→AAA)  EO946_RS01360 ← hypothetical protein
RA 443,396 G→T 6.4% G261V (GGA→GTA)  gdh → glucose 1‑dehydrogenase
RA 607,359 A→C 16.1% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,413 A→C 9.4% T86P (ACC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,671 C→G 6.5% P172A (CCA→GCA) ‡ EO946_RS03185 → collagen‑like protein
RA 607,673 A→G 5.8% P172P (CCA→CCG) ‡ EO946_RS03185 → collagen‑like protein
RA 607,674 A→C 6.8% T173P (ACC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,682 G→C 14.6% A175A (GCG→GCC EO946_RS03185 → collagen‑like protein
RA 607,688 A→G 12.3% G177G (GGA→GGG EO946_RS03185 → collagen‑like protein
RA 607,700 A→C 27.4% E181D (GAA→GAC EO946_RS03185 → collagen‑like protein
RA 607,702 C→T 19.9% A182V (GCC→GTC)  EO946_RS03185 → collagen‑like protein
RA 607,838 A→G 8.8% T227T (ACA→ACG EO946_RS03185 → collagen‑like protein
RA 704,348 Δ1 bp 100% intergenic (+170/‑14) EO946_RS03650 → / → EO946_RS03655 CamS family sex pheromone protein/phosphotransferase enzyme family protein
RA 755,531 A→C 11.6% Q22H (CAA→CAC EO946_RS03880 → rhamnogalacturonan acetylesterase
RA 767,777 C→G 5.3% G63A (GGC→GCC)  EO946_RS03930 ← hypothetical protein
RA 828,787 G→A 100% E77K (GAA→AAA)  treC → alpha,alpha‑phosphotrehalase
RA 886,754 A→C 10.4% V45G (GTT→GGT)  EO946_RS04490 ← MFS transporter
RA 1,035,865 G→T 9.8% C200F (TGC→TTC)  EO946_RS05350 → ABC transporter ATP‑binding protein
RA 1,040,035 G→C 6.0% D429H (GAT→CAT)  EO946_RS05365 → AAA family ATPase
RA 1,111,632 T→G 8.4% L576R (CTT→CGT)  addB → helicase‑exonuclease AddAB subunit AddB
RA 1,111,643 C→A 9.6% L580I (CTA→ATA)  addB → helicase‑exonuclease AddAB subunit AddB
RA 1,152,026 G→C 7.8% G59G (GGC→GGG EO946_RS05920 ← GNAT family N‑acetyltransferase
RA 1,263,953 G→T 9.7% I77I (ATC→ATA fetB ← iron export ABC transporter permease subunit FetB
RA 1,514,854 T→G 9.1% intergenic (+58/+1) EO946_RS07915 → / ← EO946_RS07920 YlaH‑like family protein/YlaI family protein
RA 1,514,935 G→T 5.5% Q44K (CAA→AAA)  EO946_RS07920 ← YlaI family protein
RA 1,936,618 G→C 6.4% L3545V (CTG→GTG)  EO946_RS09735 ← non‑ribosomal peptide synthetase
RA 1,950,238 G→A 6.5% I2995I (ATC→ATT EO946_RS09740 ← non‑ribosomal peptide synthetase
RA 1,968,161 G→T 17.6% D78Y (GAT→TAT)  EO946_RS09780 → hypothetical protein
RA 2,214,528 G→T 5.6% Q57K (CAA→AAA)  nth ← endonuclease III
RA 2,350,852 G→T 5.7% pseudogene (929/1182 nt) EO946_RS12020 ← IS3 family transposase
RA 2,379,799 T→G 6.3% K382T (AAG→ACG)  EO946_RS12160 ← sigma‑54‑dependent Fis family transcriptional regulator
RA 2,491,058 T→G 6.0% E271D (GAA→GAC floA ← flotillin‑like protein FloA
RA 2,502,212 T→G 6.4% N284H (AAC→CAC)  hrcA ← heat‑inducible transcriptional repressor HrcA
RA 2,680,491 A→C 6.3% F387V (TTC→GTC)  EO946_RS13875 ← LysM peptidoglycan‑binding domain‑containing protein
RA 2,812,790 T→G 8.4% E199D (GAA→GAC EO946_RS14520 ← SDR family oxidoreductase
RA 2,901,671 A→C 14.6% K411T (AAA→ACA)  EO946_RS14945 → extracellular solute‑binding protein
RA 2,978,569 C→T 14.9% noncoding (2232/2928 nt) EO946_RS15430 ← 23S ribosomal RNA
RA 2,979,834 C→T 10.8% noncoding (967/2928 nt) EO946_RS15430 ← 23S ribosomal RNA
RA 2,980,160 G→A 29.2% noncoding (641/2928 nt) EO946_RS15430 ← 23S ribosomal RNA
RA 2,982,236 T→C 46.2% noncoding (284/1550 nt) EO946_RS15435 ← 16S ribosomal RNA
RA 2,991,245 G→T 18.0% intergenic (+46/‑161) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA
RA 3,217,687 T→C 7.2% K166K (AAA→AAG sigO ← RNA polymerase sigma factor SigO
RA 3,217,702 A→C 7.3% L161L (CTT→CTG sigO ← RNA polymerase sigma factor SigO
RA 3,217,708 T→C 6.8% K159K (AAA→AAG sigO ← RNA polymerase sigma factor SigO
RA 3,219,740 C→A 7.9% H360N (CAT→AAT)  oxdC → oxalate decarboxylase
RA 3,219,794 G→T 7.3% V378F (GTT→TTT)  oxdC → oxalate decarboxylase
RA 3,247,065 C→T 100% intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
RA 3,280,377 G→T 7.0% Q115K (CAA→AAA)  EO946_RS16965 ← lantibiotic dehydratase
RA 3,285,567 C→T 30.7% L278L (CTG→CTA EO946_RS16995 ← IS3 family transposase
RA 3,285,575 T→G 31.4% N276H (AAT→CAT)  EO946_RS16995 ← IS3 family transposase
RA 3,285,580 A→G 34.1% I274T (ATA→ACA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,647 C→A 34.0% V252L (GTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,713 G→A 24.4% L230L (CTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,319,761 T→G 7.2% pseudogene (544/1292 nt) EO946_RS17170 ← arabinogalactan endo‑beta‑1,4‑galactanase
RA 3,369,578 C→A 16.4% G110W (GGG→TGG)  EO946_RS17410 ← HAMP domain‑containing histidine kinase
RA 3,447,038 T→G 11.2% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU
RA 3,496,387 G→T 9.2% S347R (AGC→AGA EO946_RS17995 ← glucosaminidase domain‑containing protein
RA 3,496,423 G→T 8.8% F335L (TTC→TTA EO946_RS17995 ← glucosaminidase domain‑containing protein
RA 3,584,291 G→T 5.5% S324S (TCG→TCT EO946_RS18420 → N‑acetylneuraminate synthase family protein
RA 3,584,300 G→C 7.0% Q327H (CAG→CAC EO946_RS18420 → N‑acetylneuraminate synthase family protein
RA 3,584,316 G→C 5.6% E333Q (GAA→CAA)  EO946_RS18420 → N‑acetylneuraminate synthase family protein
RA 3,719,864 T→G 7.0% S79R (AGC→CGC)  EO946_RS19145 ← YwdI family protein
RA 3,769,576 T→G 8.3% I281S (ATT→AGT)  sacX → SacY negative regulator SacX
RA 3,851,237 G→T 9.0% I46I (ATC→ATA EO946_RS19820 ← hypothetical protein
RA 3,851,254 G→C 5.1% L41V (CTT→GTT)  EO946_RS19820 ← hypothetical protein
RA 3,906,067 A→C 14.3% I42S (ATT→AGT)  EO946_RS20100 ← DUF3255 family protein
RA 3,996,857 C→A 7.1% intergenic (‑163/+43) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein
RA 3,996,927 C→A 7.2% M131I (ATG→ATT EO946_RS20530 ← VOC family protein
RA 4,022,597 G→T 5.5% V607F (GTT→TTT)  EO946_RS20675 → thioredoxin domain‑containing protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 91969NA (NA)11 (0.040) 5/250 NT NA intergenic (+131/‑39) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
?NZ_CP034943 91973 = NA (NA)intergenic (+135/‑35) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA