breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 1,844,126 Δ1 bp coding (710/2754 nt) gacS ← sensor protein GacS
RA 1,852,105 G→T K383N (AAG→AAT relA → ATP:GTP 3'‑pyrophosphotransferase
RA 4,741,283 C→G intergenic (+474/+278) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,285 2 bp→CT intergenic (+476/+275) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947 171377–172965 176327–172975 11–4951 5 [4] [4] 7 PP_16SA–[PP_5SA] PP_16SA,PP_23SA,[PP_5SA]
* * ÷ NC_002947 176674–178395 181715–178405 11–5042 5 [3] [4] 5 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947 4808200 4808244 45 5 [4] [4] 5 PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 = 176399NA (NA)12 (0.500)
+CGAAA
8/254 1.7 31.6% intergenic (+43/‑420) PP_5SA/PP_16SB 5S ribosomal RNA/16S ribosomal RNA
?NC_002947 = 182013 27 (1.090)intergenic (+2/+63) PP_5SB/PP_0160 5S ribosomal RNA/ferrioxamine receptor
* ? NC_002947 = 31273122 (0.890)7 (0.320) 7/236 1.7 24.7% coding (483/831 nt) fdhD protein sulfur transferase
?NC_002947 = 312759 23 (1.040)coding (455/831 nt) fdhD protein sulfur transferase
* ? NC_002947 = 38085820 (0.800)5 (0.240) 5/218 2.1 21.1% intergenic (+45/+64) gbcB/PP_0317 glycine‑betaine dioxygenase subunit/methyl‑accepting chemotaxis transducer
?NC_002947 = 380892 21 (1.020)intergenic (+79/+30) gbcB/PP_0317 glycine‑betaine dioxygenase subunit/methyl‑accepting chemotaxis transducer
* ? NC_002947 = 51345516 (0.640)4 (0.170) 4/246 2.7 22.3% intergenic (‑99/‑92) crp/yhfA DNA‑binding transcriptional dual regulator/hypothetical protein
?NC_002947 = 513472 13 (0.560)intergenic (‑116/‑75) crp/yhfA DNA‑binding transcriptional dual regulator/hypothetical protein
* ? NC_002947 1138537 =36 (1.450)7 (0.320) 7/236 1.7 20.8% intergenic (‑62/+103) arcC/arcB carbamate kinase/ornithine carbamoyltransferase
?NC_002947 1138626 = 21 (0.950)intergenic (‑151/+14) arcC/arcB carbamate kinase/ornithine carbamoyltransferase
* ? NC_002947 = 122814021 (0.850)6 (0.260) 5/248 2.4 21.9% coding (1314/4320 nt) PP_1072 leucine‑rich repeat‑containing protein
?NC_002947 = 1228145 23 (0.990)coding (1319/4320 nt) PP_1072 leucine‑rich repeat‑containing protein
* ? NC_002947 = 142159215 (0.600)4 (0.170) 4/252 2.7 21.4% coding (166/1860 nt) PP_1246 hypothetical protein
?NC_002947 = 1421617 15 (0.630)coding (191/1860 nt) PP_1246 hypothetical protein
* ? NC_002947 1427331 =NA (NA)5 (0.210) 5/250 2.4 NA coding (1222/1422 nt) PP_1250 group II intron‑encoding maturase
?NC_002947 1427349 = NA (NA)coding (1240/1422 nt) PP_1250 group II intron‑encoding maturase
* ? NC_002947 = 144313517 (0.680)8 (0.400) 8/214 1.3 32.8% intergenic (‑66/+110) ghrB/PP_1262 2‑ketoaldonate reductase/hydroxypyruvate/glyoxylate reductase/LysR family transcriptional regulator
?NC_002947 = 1443153 19 (0.940)intergenic (‑84/+92) ghrB/PP_1262 2‑ketoaldonate reductase/hydroxypyruvate/glyoxylate reductase/LysR family transcriptional regulator
* ? NC_002947 1487013 =28 (1.130)7 (0.360) 6/204 1.6 30.0% intergenic (+22/+108) yhdZ/degS amino acid ABC transporter ATP‑binding protein/serine endoprotease DegS
?NC_002947 1487103 = 11 (0.570)intergenic (+112/+18) yhdZ/degS amino acid ABC transporter ATP‑binding protein/serine endoprotease DegS
* ? NC_002947 = 152670719 (0.760)7 (0.300) 7/246 1.8 27.6% coding (852/957 nt) ddlB D‑alanine‑‑D‑alanine ligase B
?NC_002947 = 1526720 19 (0.820)coding (865/957 nt) ddlB D‑alanine‑‑D‑alanine ligase B
* ? NC_002947 = 178012719 (0.760)5 (0.240) 5/226 2.1 21.6% intergenic (+44/+62) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
?NC_002947 = 1780151 20 (0.940)intergenic (+68/+38) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
* ? NC_002947 1967188 =13 (0.520)5 (0.270) 5/198 1.8 31.5% intergenic (+15/+104) PP_1762/yciK hypothetical protein/putative oxidoreductase
?NC_002947 1967284 = 12 (0.640)intergenic (+111/+8) PP_1762/yciK hypothetical protein/putative oxidoreductase
* ? NC_002947 = 205786620 (0.800)4 (0.200) 4/216 2.4 20.3% intergenic (+39/+89) PP_1832/PP_1833 oxidase/hypothetical protein
?NC_002947 = 2057906 15 (0.740)intergenic (+79/+49) PP_1832/PP_1833 oxidase/hypothetical protein
* ? NC_002947 2063203 =26 (1.050)5 (0.250) 5/214 2.0 25.6% intergenic (+17/‑190) PP_1838/PP_1839 membrane protein/hypothetical protein
?NC_002947 2063297 = 8 (0.400)intergenic (+111/‑96) PP_1838/PP_1839 membrane protein/hypothetical protein
* ? NC_002947 2271258 =24 (0.970)8 (0.370) 7/232 1.7 29.0% intergenic (+36/‑184) PP_2002/PP_t31 oxidoreductase/tRNA‑Val
?NC_002947 2271306 = 18 (0.820)intergenic (+84/‑136) PP_2002/PP_t31 oxidoreductase/tRNA‑Val
* ? NC_002947 = 232947124 (0.970)7 (0.300) 5/250 2.4 23.8% coding (1016/1239 nt) PP_2047 3‑hydroxyacyl‑CoA dehydrogenase family protein
?NC_002947 = 2329476 22 (0.930)coding (1021/1239 nt) PP_2047 3‑hydroxyacyl‑CoA dehydrogenase family protein
* ? NC_002947 = 370141916 (0.640)5 (0.210) 4/254 2.7 21.6% coding (116/1053 nt) PP_3266 hypothetical protein
?NC_002947 = 3701458 21 (0.880)coding (77/1053 nt) PP_3266 hypothetical protein
* ? NC_002947 3920062 =15 (0.600)6 (0.250) 6/258 2.2 26.9% intergenic (+34/+59) mobA/acs molybdenum cofactor guanylyltransferase/acyl‑CoA synthetase
?NC_002947 3920118 = 18 (0.740)intergenic (+90/+3) mobA/acs molybdenum cofactor guanylyltransferase/acyl‑CoA synthetase
* ? NC_002947 3968919 =8 (0.320)6 (0.250) 5/256 2.4 28.7% coding (1003/1179 nt) PP_3498 transposase
?NC_002947 3969010 = 22 (0.910)coding (1094/1179 nt) PP_3498 transposase
* ? NC_002947 4021060 =19 (0.760)5 (0.210) 5/254 2.4 21.1% coding (1727/2067 nt) PP_3546 two‑component system sensor histidine kinase/response regulator
?NC_002947 4021082 = 19 (0.790)coding (1749/2067 nt) PP_3546 two‑component system sensor histidine kinase/response regulator
* ? NC_002947 4524439 =22 (0.890)10 (0.450) 8/236 1.5 34.1% intergenic (+18/‑175) idh/PP_4013 isocitrate dehydrogenase/MutT/nudix family protein
?NC_002947 4524474 = 19 (0.860)intergenic (+53/‑140) idh/PP_4013 isocitrate dehydrogenase/MutT/nudix family protein
* ? NC_002947 4586063 =5 (0.200)4 (0.230) 4/188 2.0 54.9% intergenic (+102/+115) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
?NC_002947 4586167 = 3 (0.170)intergenic (+206/+11) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
* ? NC_002947 = 45861005 (0.200)3 (0.170) 3/188 2.4 47.8% intergenic (+139/+78) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
?NC_002947 = 4586128 3 (0.170)intergenic (+167/+50) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
* ? NC_002947 = 46476417 (0.280)4 (0.230) 4/186 2.0 40.1% intergenic (+48/+83) fusB/PP_4112 elongation factor G 2/sulfate transporter
?NC_002947 = 4647671 7 (0.400)intergenic (+78/+53) fusB/PP_4112 elongation factor G 2/sulfate transporter
* ? NC_002947 4726014 =27 (1.090)6 (0.250) 6/250 2.1 21.6% intergenic (‑136/+100) PP_4183/brnQ membrane protein/branched‑chain amino acid transporter
?NC_002947 4726042 = 18 (0.760)intergenic (‑164/+72) PP_4183/brnQ membrane protein/branched‑chain amino acid transporter
* ? NC_002947 = 520166314 (0.560)7 (0.390) 5/190 1.8 38.8% intergenic (‑101/‑79) PP_4582/PP_4583 membrane protein/putative Peptidase
?NC_002947 = 5201684 12 (0.670)intergenic (‑122/‑58) PP_4582/PP_4583 membrane protein/putative Peptidase
* ? NC_002947 = 538248017 (0.680)3 (0.160) 3/194 2.5 20.3% intergenic (+52/+81) smpB/pdhR SsrA‑binding protein/DNA‑binding transcriptional regulator LldR
?NC_002947 = 5382508 11 (0.600)intergenic (+80/+53) smpB/pdhR SsrA‑binding protein/DNA‑binding transcriptional regulator LldR
* ? NC_002947 5415552 =16 (0.640)4 (0.170) 4/256 2.8 21.9% coding (273/1392 nt) gabP‑V gamma‑aminobutyrate permease
?NC_002947 5415582 = 13 (0.540)coding (243/1392 nt) gabP‑V gamma‑aminobutyrate permease
* ? NC_002947 = 5454649NA (NA)8 (0.330) 4/254 2.7 NA coding (1260/1329 nt) PP_4791 transposase
?NC_002947 5545214 = NA (NA)intergenic (‑306/+571) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
* ? NC_002947 = 553710518 (0.720)4 (0.200) 4/210 2.3 20.3% intergenic (+34/+69) nadE/PP_4870 NH(3)‑dependent NAD(+) synthetase/azurin
?NC_002947 = 5537127 17 (0.860)intergenic (+56/+47) nadE/PP_4870 NH(3)‑dependent NAD(+) synthetase/azurin
* ? NC_002947 5743392 =23 (0.930)7 (0.310) 7/238 1.7 24.2% intergenic (‑169/+78) fbp/glgP class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase
?NC_002947 5743446 = 23 (1.030)intergenic (‑223/+24) fbp/glgP class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase
* ? NC_002947 5759460 =30 (1.210)4 (0.210) 4/200 2.2 20.1% intergenic (+13/+143) trmL/secB tRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/protein‑export protein SecB
?NC_002947 5759552 = 9 (0.480)intergenic (+105/+51) trmL/secB tRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/protein‑export protein SecB
* ? NC_002947 5914213 =27 (1.090)5 (0.260) 5/208 2.0 22.1% intergenic (+49/+104) spuI/argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase
?NC_002947 5914309 = 14 (0.720)intergenic (+145/+8) spuI/argA glutamylpolyamine synthetase/amino acid N‑acetyltransferase
* ? NC_002947 6006595 =30 (1.210)11 (0.610) 11/192 0.7 47.0% intergenic (+7/+92) ydcJ/PP_5261 metalloprotein/LysR family transcriptional regulator
?NC_002947 6006679 = 3 (0.170)intergenic (+91/+8) ydcJ/PP_5261 metalloprotein/LysR family transcriptional regulator