breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 33,060 G→A 5.6% A748A (GCG→GCA carB → carbamoyl‑phosphate synthase large subunit
RA 37,454 T→A 7.5% Q124L (CAA→CTA)  caiC ← putative crotonobetaine/carnitine‑CoA ligase
RA 59,299 G→T 5.1% intergenic (+20/+388) yabP → / ← rluA pseudogene, pentapeptide repeats‑containing/dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent
RA 59,301 A→C 5.2% intergenic (+22/+386) yabP → / ← rluA pseudogene, pentapeptide repeats‑containing/dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM‑dependent
RA 78,830 T→A 6.8% intergenic (+31/+18) setA → / ← leuD broad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit
RA 78,831 T→A 6.5% intergenic (+32/+17) setA → / ← leuD broad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit
RA 87,602 T→C 5.1% V82V (GTT→GTC ilvH → acetolactate synthase 3, small subunit, valine‑sensitive
RA 92,965 T→C 5.3% V518A (GTT→GCT) ‡ ftsI → transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3
RA 92,966 T→A 5.6% V518V (GTT→GTA) ‡ ftsI → transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3
RA 92,967 G→A 5.4% A519T (GCG→ACG)  ftsI → transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3
RA 120,154 Δ1 bp 5.3% intergenic (+19/+24) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
RA 120,158:1 +A 5.4% intergenic (+23/+20) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
RA 151,519 A→C 6.4% I27M (ATT→ATG yadK ← putative fimbrial‑like adhesin protein
RA 151,522 G→T 6.5% A26A (GCC→GCA yadK ← putative fimbrial‑like adhesin protein
RA 164,620 C→T 5.3% intergenic (+86/‑110) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 164,621 A→G 5.3% intergenic (+87/‑109) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 167,842:1 +T 71.1% coding (359/2244 nt) fhuA → ferrichrome outer membrane transporter
RA 168,284 C→A 23.2% Y267* (TAC→TAA fhuA → ferrichrome outer membrane transporter
RA 184,180 C→A 5.3% intergenic (‑85/+77) yaeH ← / ← yaeI UPF0325 family protein/phosphodiesterase with model substrate bis‑pNPP
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 289,256 G→C 6.2% intergenic (‑94/+45) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
RA 289,257 T→A 6.2% intergenic (‑95/+44) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
RA 317,566 G→A 5.4% pseudogene (128/129 nt) ykgQ → pseudogene, putative dehydrogenase
RA 317,571 T→C 5.2% intergenic (+4/+155) ykgQ → / ← rclC pseudogene, putative dehydrogenase/reactive chlorine species (RCS) stress resistance inner membrane protein
RA 326,474 T→C 5.2% A258A (GCA→GCG betA ← choline dehydrogenase, a flavoprotein
RA 355,892 G→A 5.5% W324* (TGG→TAG)  codB → cytosine transporter
RA 383,897 C→T 5.4% M13I (ATG→ATA) ‡ yaiP ← putative family 2 glycosyltransferase
RA 383,898 A→G 5.4% M13T (ATG→ACG) ‡ yaiP ← putative family 2 glycosyltransferase
RA 400,634 G→A 5.0% P97L (CCG→CTG)  ddlA ← D‑alanine‑D‑alanine ligase A
RA 453,467 G→A 5.0% P27S (CCG→TCG)  ampG ← muropeptide transporter
RA 454,853 T→C 5.9% intergenic (+64/‑280) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
RA 454,856 G→A 5.9% intergenic (+67/‑277) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
RA 470,313 C→G 7.2% I481M (ATC→ATG mdlA → putative multidrug ABC transporter ATPase
RA 481,225 T→G 6.3% intergenic (‑517/+29) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
RA 481,228 C→A 5.3% intergenic (‑520/+26) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
RA 483,206 G→A 5.9% L400F (CTC→TTC)  acrB ← multidrug efflux system protein
RA 517,379 C→A 5.1% intergenic (+17/+46) fetB → / ← ybbN iron export ABC transporter permease; peroxide resistance protein/DnaK co‑chaperone, thioredoxin‑like protein
RA 517,380 A→T 5.1% intergenic (+18/+45) fetB → / ← ybbN iron export ABC transporter permease; peroxide resistance protein/DnaK co‑chaperone, thioredoxin‑like protein
RA 517,384 T→G 5.1% intergenic (+22/+41) fetB → / ← ybbN iron export ABC transporter permease; peroxide resistance protein/DnaK co‑chaperone, thioredoxin‑like protein
RA 522,634 G→A 5.0% G740D (GGC→GAC)  ybbP → putative ABC transporter permease
RA 551,678 T→C 5.0% D306G (GAC→GGC)  purK ← N5‑carboxyaminoimidazole ribonucleotide synthase
RA 556,640 T→A 11.2% intergenic (‑87/+21) ybcI ← / ← ybcJ DUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
RA 556,641 G→T 8.2% intergenic (‑88/+20) ybcI ← / ← ybcJ DUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
RA 556,642 A→C 8.1% intergenic (‑89/+19) ybcI ← / ← ybcJ DUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
RA 556,643 T→A 9.4% intergenic (‑90/+18) ybcI ← / ← ybcJ DUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
RA 717,518 G→A 5.8% P250S (CCA→TCA)  potE ← putrescine/proton symporter: putrescine/ornithine antiporter
RA 790,928 C→T 6.2% Q34Q (CAG→CAA) ‡ galT ← galactose‑1‑phosphate uridylyltransferase
RA 790,929 T→G 6.1% Q34P (CAG→CCG) ‡ galT ← galactose‑1‑phosphate uridylyltransferase
RA 790,932 C→A 6.3% W33L (TGG→TTG) ‡ galT ← galactose‑1‑phosphate uridylyltransferase
RA 790,933 A→G 6.3% W33R (TGG→CGG) ‡ galT ← galactose‑1‑phosphate uridylyltransferase
RA 837,162 G→A 5.9% C271Y (TGC→TAC)  ybiC → putative dehydrogenase
RA 869,724 A→C 5.7% N105T (AAC→ACC)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 869,735 G→T 5.5% V109L (GTG→TTG)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 909,422 G→A 76.6% A543V (GCC→GTC)  poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
JC JC 911,086 IS2 (–) +5 bp 68.6% intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
RA 911,148 C→T 8.5% intergenic (‑99/+34) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
RA 922,314:1 +C 5.5% intergenic (+21/+52) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
RA 940,751 T→C 5.3% intergenic (+31/‑208) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
RA 980,167 A→T 5.6% E1281V (GAA→GTA)  mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit
RA 1,018,249 G→C 5.7% S17R (AGC→AGG) ‡ ycbZ ← putative peptidase
RA 1,018,251 T→G 5.5% S17R (AGC→CGC) ‡ ycbZ ← putative peptidase
RA 1,018,254 C→A 5.6% D16Y (GAT→TAT)  ycbZ ← putative peptidase
RA 1,018,256 G→C 5.6% T15S (ACC→AGC)  ycbZ ← putative peptidase
RA 1,153,219 G→A 5.4% intergenic (+39/‑81) fabF → / → pabC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II/4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
RA 1,163,979 G→A 5.2% L240L (CTG→CTA thiK → thiamine kinase
RA 1,188,406 C→T 13.7% E457E (GAG→GAA phoQ ← sensory histidine kinase in two‑component regulatory system with PhoP
RA 1,196,220 C→T 15.4% H366H (CAC→CAT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,232 C→T 12.8% T370T (ACC→ACT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,245 T→C 12.3% L375L (TTA→CTA) ‡ icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,247 A→G 12.2% L375L (TTA→TTG) ‡ icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,280 G→A 5.8% A386A (GCG→GCA icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,228,183 C→T 5.1% S35S (AGC→AGT ycgM → putative isomerase/hydrolase
RA 1,240,000 T→C 7.3% intergenic (+51/+335) dadX → / ← cvrA alanine racemase, catabolic, PLP‑binding/putative cation/proton antiporter
RA 1,271,320 A→T 6.3% I177N (ATT→AAT)  chaA ← calcium/sodium:proton antiporter
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,335,681 T→C 5.1% intergenic (+223/‑150) yciX → / → acnA uncharacterized protein/aconitate hydratase 1; aconitase A
RA 1,335,684 G→A 5.6% intergenic (+226/‑147) yciX → / → acnA uncharacterized protein/aconitate hydratase 1; aconitase A
RA 1,368,966 C→T 5.5% R56C (CGT→TGT)  pspB → psp operon transcription co‑activator
RA 1,440,441 T→C 5.0% H115R (CAT→CGT)  pfo ← pyruvate‑flavodoxin oxidoreductase
RA 1,465,671 C→G 5.1% pseudogene (280/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,549,000 A→T 5.5% T534S (ACC→TCC)  fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,552,078 G→A 5.5% intergenic (+87/+320) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
RA 1,552,079 C→A 5.5% intergenic (+88/+319) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
RA 1,570,252 A→T 6.5% S80T (TCA→ACA)  gadC ← glutamate:gamma‑aminobutyric acid antiporter
RA 1,570,610 G→A 6.0% intergenic (‑121/+35) gadC ← / ← gadB glutamate:gamma‑aminobutyric acid antiporter/glutamate decarboxylase B, PLP‑dependent
RA 1,570,611 G→A 5.9% intergenic (‑122/+34) gadC ← / ← gadB glutamate:gamma‑aminobutyric acid antiporter/glutamate decarboxylase B, PLP‑dependent
RA 1,570,612 T→C 5.7% intergenic (‑123/+33) gadC ← / ← gadB glutamate:gamma‑aminobutyric acid antiporter/glutamate decarboxylase B, PLP‑dependent
RA 1,570,613 T→C 5.7% intergenic (‑124/+32) gadC ← / ← gadB glutamate:gamma‑aminobutyric acid antiporter/glutamate decarboxylase B, PLP‑dependent
RA 1,571,721 G→A 8.0% H109Y (CAT→TAT)  gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,698,585 T→G 6.1% E199A (GAA→GCA)  malI ← transcriptional repressor of Mal regulon
RA 1,698,587 T→A 6.2% A198A (GCA→GCT) ‡ malI ← transcriptional repressor of Mal regulon
RA 1,698,589 C→A 6.3% A198S (GCA→TCA) ‡ malI ← transcriptional repressor of Mal regulon
RA 1,708,805 T→A 19.7% R629R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,743,598 G→A 5.3% A48T (GCT→ACT)  mdtK → multidrug efflux system transporter
RA 1,789,497 T→G 5.8% intergenic (+16/‑116) aroH → / → ydiE 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/hemin uptake protein HemP homolog
RA 1,789,498 G→T 6.2% intergenic (+17/‑115) aroH → / → ydiE 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/hemin uptake protein HemP homolog
RA 1,789,499 A→C 6.4% intergenic (+18/‑114) aroH → / → ydiE 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/hemin uptake protein HemP homolog
RA 1,789,500 C→A 5.5% intergenic (+19/‑113) aroH → / → ydiE 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/hemin uptake protein HemP homolog
RA 1,796,340 G→A 28.3% S535S (AGC→AGT pheT ← phenylalanine tRNA synthetase, beta subunit
RA 1,810,062 T→C 9.0% intergenic (+14/‑149) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,810,065 T→G 9.7% intergenic (+17/‑146) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,810,066 C→A 8.8% intergenic (+18/‑145) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,810,069 G→A 9.0% intergenic (+21/‑142) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,835,576 G→A 5.5% R21H (CGT→CAT)  ynjA → carboxymuconolactone decarboxylase family protein
RA 1,840,795 G→A 8.3% L203L (CTC→CTT ynjF ← CDP‑alcohol phosphatidyltransferase family inner membrane protein
RA 1,840,798 C→T 10.5% S202S (TCG→TCA) ‡ ynjF ← CDP‑alcohol phosphatidyltransferase family inner membrane protein
RA 1,840,799 G→C 8.8% S202W (TCG→TGG) ‡ ynjF ← CDP‑alcohol phosphatidyltransferase family inner membrane protein
RA 1,840,800 A→G 8.9% S202P (TCG→CCG) ‡ ynjF ← CDP‑alcohol phosphatidyltransferase family inner membrane protein
RA 1,840,803 T→C 9.1% K201E (AAG→GAG)  ynjF ← CDP‑alcohol phosphatidyltransferase family inner membrane protein
RA 1,849,031 T→C 5.6% G65G (GGT→GGC sppA → protease IV (signal peptide peptidase)
RA 1,916,621 T→C 5.1% F122L (TTT→CTT)  yebS → inner membrane PqiA domain protein
RA 1,919,474 T→C 5.3% Y655Y (TAT→TAC yebT → MCE domain protein
RA 1,926,882 C→A 7.4% G653V (GGC→GTC)  ptrB ← protease II
RA 1,926,894 T→G 6.2% D649A (GAC→GCC)  ptrB ← protease II
RA 1,936,091 T→C 5.3% D75G (GAT→GGT)  zwf ← glucose‑6‑phosphate 1‑dehydrogenase
RA 1,959,047 C→T 6.0% A74T (GCC→ACC)  cutC ← copper homeostasis protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 1,981,762 A→C 5.1% V209G (GTT→GGT)  otsB ← trehalose‑6‑phosphate phosphatase, biosynthetic
RA 1,987,857 C→T 6.6% intergenic (‑99/+16) yecJ ← / ← azuC DUF2766 family protein/acid‑inducible small membrane‑associated protein
RA 1,987,858 G→C 7.5% intergenic (‑100/+15) yecJ ← / ← azuC DUF2766 family protein/acid‑inducible small membrane‑associated protein
RA 1,987,859 G→C 6.7% intergenic (‑101/+14) yecJ ← / ← azuC DUF2766 family protein/acid‑inducible small membrane‑associated protein
RA 1,987,860 A→G 6.7% intergenic (‑102/+13) yecJ ← / ← azuC DUF2766 family protein/acid‑inducible small membrane‑associated protein
RA 2,008,719 C→G 5.9% A148G (GCC→GGC)  yedE → UPF0394 family sulphur transport domain‑containing inner membrane protein
RA 2,045,401 A→G 5.2% D155G (GAT→GGT)  yeeJ → putative adhesin
RA 2,066,248 C→T 8.0% intergenic (‑94/+57) yeeH ← / ← insH1 pseudogene/IS5 transposase and trans‑activator
RA 2,079,004 G→C 6.1% intergenic (+73/+28) yoeF → / ← yeeX pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related/UPF0265 family protein
RA 2,120,198 T→C 5.2% T453A (ACG→GCG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase
RA 2,139,898 A→G 5.2% L572P (CTC→CCC)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,216,157 A→C 5.2% V107G (GTT→GGT)  yehW ← putative ABC transporter permease
RA 2,217,066 G→A 5.5% I114I (ATC→ATT yehX ← putative ABC transporter ATPase
RA 2,236,657 G→A 7.0% intergenic (+157/+86) preA → / ← mglC dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C/methyl‑galactoside transporter subunit
RA 2,236,658 C→A 7.0% intergenic (+158/+85) preA → / ← mglC dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C/methyl‑galactoside transporter subunit
RA 2,236,664 G→T 6.2% intergenic (+164/+79) preA → / ← mglC dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C/methyl‑galactoside transporter subunit
RA 2,236,665 A→C 6.2% intergenic (+165/+78) preA → / ← mglC dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C/methyl‑galactoside transporter subunit
RA 2,236,671 T→G 5.9% intergenic (+171/+72) preA → / ← mglC dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C/methyl‑galactoside transporter subunit
RA 2,236,672 T→C 6.0% intergenic (+172/+71) preA → / ← mglC dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C/methyl‑galactoside transporter subunit
RA 2,286,311 C→G 8.6% intergenic (+24/+79) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,286,313 T→A 8.4% intergenic (+26/+77) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,286,315 C→G 8.5% intergenic (+28/+75) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,305,085 A→G 8.0% intergenic (+692/+23) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,086 C→G 7.9% intergenic (+693/+22) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,087 C→T 8.0% intergenic (+694/+21) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,394,036 G→T 5.6% T337T (ACC→ACA nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L
RA 2,411,925 A→T 5.3% F55L (TTT→TTA) ‡ yfbT ← sugar phosphatas
RA 2,411,926 A→T 5.3% F55Y (TTT→TAT) ‡ yfbT ← sugar phosphatas
RA 2,411,927 A→T 5.3% F55I (TTT→ATT) ‡ yfbT ← sugar phosphatas
RA 2,416,375 G→T 17.8% M543I (ATG→ATT pta → phosphate acetyltransferase
RA 2,448,750 C→T 11.1% Q49* (CAG→TAG)  smrB → putative DNA endonuclease
RA 2,454,260 G→A 5.5% pseudogene (742/2646 nt) yfcU ← pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein
RA 2,474,878 T→C 5.8% intergenic (+22/‑106) yfdQ → / → yfdR CPS‑53 (KpLE1) prophage; uncharacterized protein/CPS‑53 (KpLE1) prophage; conserved protein
RA 2,521,344 C→T 5.7% intergenic (+16/+249) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB
RA 2,521,346 A→T 5.1% intergenic (+18/+247) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB
RA 2,521,348 A→G 5.2% intergenic (+20/+245) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB
RA 2,613,332 C→T 5.5% P478L (CCG→CTG)  hyfR → hydrogenase‑4 transcriptional activator
RA 2,684,083 C→T 8.1% intergenic (+27/+171) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,684,084 G→T 6.1% intergenic (+28/+170) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,684,085 A→C 6.1% intergenic (+29/+169) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,684,086 A→G 6.0% intergenic (+30/+168) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,707,320 A→T 5.6% intergenic (‑196/+2) lepA ← / ← rseC back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component
RA 2,707,321 A→T 5.6% intergenic (‑197/+1) lepA ← / ← rseC back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component
RA 2,720,413 C→T 25.6% S154L (TCG→TTG)  pka → protein lysine acetyltransferase
JC 2,720,855 +GCGCTG 68.5% coding (903/2661 nt) pka → protein lysine acetyltransferase
RA 2,729,465 G→A 9.9% intergenic (‑21/+151) gltW ← / ← rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
RA 2,729,466 T→A 9.8% intergenic (‑22/+150) gltW ← / ← rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
RA 2,729,467 T→C 10.0% intergenic (‑23/+149) gltW ← / ← rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
RA 2,801,056 A→T 7.3% N31I (AAT→ATT)  nrdI → NrdEF cluster assembly flavodoxin
RA 2,866,487 G→C 7.1% intergenic (+22/+72) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor
RA 2,866,488 G→C 7.2% intergenic (+23/+71) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor
RA 2,900,322 C→T 5.2% intergenic (‑49/‑270) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,900,324 G→T 6.5% intergenic (‑51/‑268) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,900,327 A→C 5.4% intergenic (‑54/‑265) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
RA 2,906,607 C→T 7.4% intergenic (+24/+36) ygcG → / ← eno TPM domain protein, putative phosphatase/enolase
RA 2,906,608 G→C 7.1% intergenic (+25/+35) ygcG → / ← eno TPM domain protein, putative phosphatase/enolase
RA 2,906,609 G→C 7.1% intergenic (+26/+34) ygcG → / ← eno TPM domain protein, putative phosphatase/enolase
RA 2,987,103 C→T 5.7% intergenic (+27/‑433) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator
RA 2,987,104 T→A 5.7% intergenic (+28/‑432) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator
RA 2,987,105 A→G 5.5% intergenic (+29/‑431) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator
RA 3,020,510 G→T 9.3% intergenic (+21/‑30) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase
RA 3,020,511 C→G 9.4% intergenic (+22/‑29) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase
RA 3,020,512 A→C 9.3% intergenic (+23/‑28) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase
RA 3,068,929 A→C 6.3% intergenic (‑121/+18) argO ← / ← mscS arginine transporter/mechanosensitive channel protein, small conductance
RA 3,087,890 C→G 7.7% intergenic (+30/‑394) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter
RA 3,087,891 T→C 7.9% intergenic (+31/‑393) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter
RA 3,087,892 C→G 6.9% intergenic (+32/‑392) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter
RA 3,087,893 G→A 8.0% intergenic (+33/‑391) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter
RA 3,087,894 C→G 7.8% intergenic (+34/‑390) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter
RA 3,109,464 C→T 5.6% intergenic (‑309/‑89) speC ← / → yqgA ornithine decarboxylase, constitutive/DUF554 family putative inner membrane protein
RA 3,169,260 Δ1 bp 5.7% intergenic (‑29/+24) ygiV ← / ← ygiW transcriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein
RA 3,169,265:1 +T 5.5% intergenic (‑34/+19) ygiV ← / ← ygiW transcriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein
RA 3,206,675 T→G 5.0% C71W (TGT→TGG ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,269,956 T→C 8.4% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,288,032 C→A 5.1% intergenic (+22/‑58) yraH → / → yraI putative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone
RA 3,291,109 A→T 5.9% K766* (AAA→TAA)  yraJ → putative outer membrane protein
RA 3,313,207 G→A 7.9% intergenic (‑29/+135) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2
RA 3,313,208 T→A 8.1% intergenic (‑30/+134) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2
RA 3,313,209 T→C 8.1% intergenic (‑31/+133) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2
RA 3,334,703 A→G 6.1% intergenic (+22/‑206) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator
RA 3,334,704 G→C 6.1% intergenic (+23/‑205) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator
RA 3,334,705 C→T 6.1% intergenic (+24/‑204) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator
RA 3,368,219 G→A 5.0% T131T (ACG→ACA yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,377,923 A→G 5.2% R95R (CGT→CGC rpsI ← 30S ribosomal subunit protein S9
RA 3,477,878 T→A 6.3% intergenic (+20/+29) slyX → / ← slyD phi X174 lysis protein/FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)
RA 3,488,983 C→A 7.6% V400L (GTG→TTG)  argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
RA 3,488,989 G→T 8.2% H398N (CAC→AAC)  argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,755,082 T→A 5.4% Y477F (TAC→TTC)  aldB ← aldehyde dehydrogenase B
RA 3,768,131 A→T 100% pseudogene (911/945 nt) rhsJ → Rhs‑family protein
RA 3,923,717 G→A 5.3% intergenic (‑37/+27) rsmG ← / ← mnmG 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,923,718 G→C 5.3% intergenic (‑38/+26) rsmG ← / ← mnmG 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,923,719 G→C 5.3% intergenic (‑39/+25) rsmG ← / ← mnmG 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,923,720 T→C 5.2% intergenic (‑40/+24) rsmG ← / ← mnmG 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,959,480 A→T 5.1% intergenic (+35/+52) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,964,929 C→T 100% E234E (GAG→GAA rhlB ← ATP‑dependent RNA helicase
RA 4,035,280 G→A 10.6% intergenic (+127/‑251) hemG → / → rrsA protoporphyrin oxidase, flavoprotein/16S ribosomal RNA of rrnA operon
RA 4,035,287 C→A 13.3% intergenic (+134/‑244) hemG → / → rrsA protoporphyrin oxidase, flavoprotein/16S ribosomal RNA of rrnA operon
RA 4,035,288 T→G 13.8% intergenic (+135/‑243) hemG → / → rrsA protoporphyrin oxidase, flavoprotein/16S ribosomal RNA of rrnA operon
RA 4,094,327 A→G 5.4% intergenic (‑55/+396) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,094,328 A→C 5.4% intergenic (‑56/+395) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,094,334 T→G 5.9% intergenic (‑62/+389) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,094,335 C→A 5.5% intergenic (‑63/+388) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,118,828 T→G 6.6% intergenic (+68/+17) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
RA 4,118,829 T→C 6.5% intergenic (+69/+16) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
RA 4,118,830 G→A 6.5% intergenic (+70/+15) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
RA 4,118,831 C→A 7.2% intergenic (+71/+14) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
RA 4,183,261 C→T 20.0% H673Y (CAC→TAC)  rpoB → RNA polymerase, beta subunit
RA 4,184,540 A→C 50.7% N1099T (AAC→ACC)  rpoB → RNA polymerase, beta subunit
RA 4,296,060 C→T 30.6% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,335,654 T→A 5.7% intergenic (‑64/+40) eptA ← / ← adiC lipid A phosphoethanolamine transferase/arginine:agmatine antiporter
RA 4,335,655 T→A 5.6% intergenic (‑65/+39) eptA ← / ← adiC lipid A phosphoethanolamine transferase/arginine:agmatine antiporter
RA 4,338,130 G→C 6.9% intergenic (‑201/+124) adiY ← / ← adiA adi system transcriptional activator/arginine decarboxylase
RA 4,376,280 G→A 5.7% intergenic (+15/‑37) efp → / → ecnA polyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B
RA 4,376,291 T→C 5.7% intergenic (+26/‑26) efp → / → ecnA polyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B
RA 4,560,590 A→G 5.2% F31L (TTT→CTT)  yjiH ← nucleoside recognition pore and gate family putative inner membrane transporter
RA 4,627,879 C→T 6.0% H189Y (CAT→TAT)  nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 123049 127564 4516 38 [37] [37] 38 [aceE]–[aceF] [aceE],[aceF]
* * ÷ NC_000913 3423753–3424234 3424614–3424238 5–862 38 [34] [37] 39 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3619318–3621083 3688673 67591–69356 38 [34] [2] 106 [rhsB]–[bcsC] [rhsB],yhhH,yrhC,yhhI,yhhJ,rbbA,yhiI,yhiJ,yhiL,yhiL,yhiM,yhiN,pitA,uspB,uspA,dtpB,rsmJ,prlC,rlmJ,gor,dinQ,agrA,agrB,arsR,arsB,arsC,yhiS,insH1,yhiS,slp,dctR,yhiD,hdeB,hdeA,hdeD,arrS,gadE,mdtE,mdtF,gadW,gadY,gadX,gadA,yhjA,treF,yhjB,yhjC,yhjD,yhjE,yhjG,yhjH,kdgK,yhjJ,dctA,yhjK,[bcsC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 15518 =NA (NA)3 (0.030) 3/240 NT NA noncoding (132/1345 nt) IS186 repeat region
?NC_000913 15620 = NA (NA)noncoding (234/1345 nt) IS186 repeat region
* ? NC_000913 = 11450584 (0.830)6 (0.070) 6/234 NT 7.0% intergenic (+18/+17) guaC/hofC GMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein
?NC_000913 = 114511 84 (0.920)intergenic (+24/+11) guaC/hofC GMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein
* ? NC_000913 225078 =NA (NA)15 (0.160) 13/244 NT NA noncoding (1308/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 225100 = NA (NA)noncoding (1330/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 = 225281NA (NA)11 (0.110) 10/248 NT NA noncoding (1511/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 = 225293 NA (NA)noncoding (1523/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 = 227444NA (NA)5 (0.050) 5/248 NT NA noncoding (1686/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 = 227459 NA (NA)noncoding (1701/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 = 315572NA (NA)3 (0.030) 3/238 NT NA noncoding (344/1255 nt) IS3 repeat region
?NC_000913 = 315588 NA (NA)noncoding (360/1255 nt) IS3 repeat region
* ? NC_000913 1115624 =NA (NA)5 (0.050) 5/236 NT NA intergenic (‑134/+38) mdtG/lpxL putative drug efflux system protein/lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase
?NC_000913 1115655 = NA (NA)intergenic (‑165/+7) mdtG/lpxL putative drug efflux system protein/lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase
* ? NC_000913 = 112349690 (0.890)6 (0.060) 4/248 NT 6.1% coding (559/648 nt) grxB glutaredoxin 2 (Grx2)
?NC_000913 = 1123525 98 (1.010)coding (530/648 nt) grxB glutaredoxin 2 (Grx2)
* ? NC_000913 1205159 =71 (0.710)4 (0.040) 4/238 NT 5.6% pseudogene (756/1134 nt) aaaE pseudogene, phage terminase protein A family, e14 prophage;Phage or Prophage Related
?NC_000913 1205208 = 70 (0.750)pseudogene (805/1134 nt) aaaE pseudogene, phage terminase protein A family, e14 prophage;Phage or Prophage Related
* ? NC_000913 1207790 =35 (0.350)18 (0.200) 17/226 NT 32.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 43 (0.490)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780536 (0.360)15 (0.170) 15/226 NT 28.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 43 (0.490)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 168123680 (0.790)5 (0.050) 4/246 NT 6.8% coding (261/723 nt) folM dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme
?NC_000913 = 1681277 61 (0.640)coding (302/723 nt) folM dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme
* ? NC_000913 1784702 =99 (0.980)6 (0.060) 5/242 NT 6.1% intergenic (+25/+32) fadK/ppsA short chain acyl‑CoA synthetase, anaerobic/phosphoenolpyruvate synthase
?NC_000913 1784727 = 93 (0.980)intergenic (+50/+7) fadK/ppsA short chain acyl‑CoA synthetase, anaerobic/phosphoenolpyruvate synthase
* ? NC_000913 2032326 =77 (0.760)4 (0.040) 4/258 NT 5.0% intergenic (‑9/+58) dcm/yedJ DNA cytosine methyltransferase/putative HD superfamily phosphohydrolase
?NC_000913 2032347 = 75 (0.740)intergenic (‑30/+37) dcm/yedJ DNA cytosine methyltransferase/putative HD superfamily phosphohydrolase
* ? NC_000913 = 217667864 (0.640)4 (0.040) 4/246 NT 5.7% coding (527/855 nt) gatY D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit
?NC_000913 = 2176710 71 (0.740)coding (495/855 nt) gatY D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit
* ? NC_000913 2231069 =79 (0.780)4 (0.040) 4/244 NT 5.2% coding (51/696 nt) yohK LrgB family inner membrane protein
?NC_000913 2231091 = 70 (0.740)coding (73/696 nt) yohK LrgB family inner membrane protein
* ? NC_000913 2729443 =42 (0.420)7 (0.080) 5/238 NT 13.2% noncoding (2/76 nt) gltW tRNA‑Glu
?NC_000913 = 4168331 53 (0.570)intergenic (+131/‑41) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
* ? NC_000913 2878074 =82 (0.810)5 (0.050) 5/244 NT 6.0% intergenic (+456/+495) iap/ygbF aminopeptidase in alkaline phosphatase isozyme conversion/CRISPR adaptation ssRNA endonuclease
?NC_000913 2878092 = 80 (0.840)intergenic (+474/+477) iap/ygbF aminopeptidase in alkaline phosphatase isozyme conversion/CRISPR adaptation ssRNA endonuclease
* ? NC_000913 = 306892174 (0.730)4 (0.040) 5/240 NT 5.2% intergenic (‑113/+26) argO/mscS arginine transporter/mechanosensitive channel protein, small conductance
?NC_000913 = 3068929 76 (0.810)intergenic (‑121/+18) argO/mscS arginine transporter/mechanosensitive channel protein, small conductance
* ? NC_000913 3087880 =NA (NA)6 (0.060) 5/238 NT NA intergenic (+20/‑404) metK/galP S‑adenosylmethionine synthetase/D‑galactose transporter
?NC_000913 3087905 = NA (NA)intergenic (+45/‑379) metK/galP S‑adenosylmethionine synthetase/D‑galactose transporter
* ? NC_000913 = 333937963 (0.630)5 (0.050) 5/242 NT 7.4% coding (687/810 nt) mlaF ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
?NC_000913 = 3339417 66 (0.700)coding (649/810 nt) mlaF ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
* ? NC_000913 3688674 =2 (0.020)90 (0.900) 67/256 NT 67.7% coding (500/3474 nt) bcsC cellulose synthase subunit
?NC_000913 = 4506221 84 (0.840)coding (935/1119 nt) yjhC GFO/IDH/MOCA family putative oxidoreductase. NAD(P)‑dependent