breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 114,506 G→T 5.2% intergenic (+19/+16) guaC → / ← hofC GMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein
RA 114,507 A→T 5.1% intergenic (+20/+15) guaC → / ← hofC GMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein
RA 126,060 A→G 5.2% E122E (GAA→GAG aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,075 T→G 6.5% V127V (GTT→GTG aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,081 T→C 7.4% D129D (GAT→GAC aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,093 T→C 5.6% A133A (GCT→GCC aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,392 C→A 6.6% A233D (GCC→GAC)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,405 A→G 8.8% S237S (TCA→TCG aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 177,405 G→T 5.8% L74M (CTG→ATG)  yadS ← UPF0126 family inner membrane protein
RA 218,544 C→A 5.9% D78Y (GAT→TAT)  proS ← prolyl‑tRNA synthetase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 394,473 G→T 5.0% intergenic (+55/‑33) yaiT → / → yaiV pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein/putative transcriptional regulator
RA 464,354 C→A 6.5% intergenic (+46/‑48) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 464,356 A→T 6.1% intergenic (+48/‑46) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 464,358 T→G 5.7% intergenic (+50/‑44) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
RA 657,533 A→T 5.6% intergenic (+32/+22) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
RA 756,150 C→T 100% Q82* (CAG→TAG)  sdhA → succinate dehydrogenase, flavoprotein subunit
RA 852,857 A→C 5.0% intergenic (‑260/+12) opgE ← / ← mntS OPG biosynthetic transmembrane phosphoethanolamine transferase/Mn(2)‑response protein, MntR‑repressed
RA 857,576 T→C 6.0% intergenic (+21/+220) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 857,578 C→G 6.2% intergenic (+23/+218) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 892,455 G→C 7.4% V163V (GTG→GTC rimK → ribosomal protein S6 modification protein
RA 892,457 T→A 7.5% I164N (ATT→AAT)  rimK → ribosomal protein S6 modification protein
RA 892,459 G→C 7.4% D165H (GAC→CAC)  rimK → ribosomal protein S6 modification protein
RA 909,610 G→T 7.0% T480T (ACC→ACA poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
RA 922,314:1 +C 6.3% intergenic (+21/+52) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
RA 925,581 A→C 5.2% intergenic (+41/+303) clpA → / ← serW ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity/tRNA‑Ser
RA 1,217,126 C→A 9.0% intergenic (+130/‑202) ymgC → / → ycgG Blue light, low temperature and stress induced protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 1,286,793 A→G 8.8% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,287,420 G→C 5.3% intergenic (‑92/+118) tyrV ← / ← tyrT tRNA‑Tyr/tRNA‑Tyr
RA 1,314,682 A→T 7.7% intergenic (+24/+36) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
RA 1,314,683 T→A 7.5% intergenic (+25/+35) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
RA 1,314,684 A→T 7.1% intergenic (+26/+34) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
RA 1,324,837 A→C 5.2% Y31S (TAT→TCT) ‡ yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,324,838 T→A 5.3% Y31* (TAT→TAA) ‡ yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,324,839 G→T 5.1% E32* (GAA→TAA)  yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,466,138 G→A 7.1% pseudogene (747/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,144 G→T 8.8% pseudogene (753/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,201 T→C 6.6% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,517,055 C→A 5.2% intergenic (+53/+44) patD → / ← yncL gamma‑aminobutyraldehyde dehydrogenase/stress‑induced small inner membrane enterobacterial protein
RA 1,552,191 C→A 21.7% intergenic (+200/+207) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
RA 1,567,466 G→C 6.2% intergenic (‑326/+38) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
RA 1,567,467 G→T 5.4% intergenic (‑327/+37) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
RA 1,567,469 A→T 5.5% intergenic (‑329/+35) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
RA 1,567,471 A→C 5.9% intergenic (‑331/+33) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
RA 1,567,472 G→C 5.5% intergenic (‑332/+32) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
RA 1,736,097 T→A 5.2% intergenic (+138/+24) sodB → / ← ydhP superoxide dismutase, Fe/putative MFS transporter, inner membrane protein
RA 1,864,763 A→T 5.4% intergenic (+29/+19) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,928,799 A→C 6.2% L14R (CTT→CGT)  ptrB ← protease II
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,001,927 G→C 5.0% intergenic (‑138/+183) fliA ← / ← fliC RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
RA 2,041,173 C→A 5.1% intergenic (+55/‑202) yedZ → / → zinT inner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic
RA 2,160,882 A→C 10.0% T833P (ACG→CCG)  mdtC → multidrug efflux system, subunit C
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,357,665 G→A 9.4% G254G (GGG→GGA yfaD → transposase_31 family protein
RA 2,405,389 G→A 6.4% intergenic (‑317/+314) nuoA ← / ← lrhA NADH:ubiquinone oxidoreductase, membrane subunit A/transcriptional repressor of flagellar, motility and chemotaxis genes
RA 2,474,881 G→A 5.8% intergenic (+25/‑103) yfdQ → / → yfdR CPS‑53 (KpLE1) prophage; uncharacterized protein/CPS‑53 (KpLE1) prophage; conserved protein
RA 2,477,022 T→G 7.0% D203A (GAC→GCC)  dsdC ← dsd operon activator; autorepressor
RA 2,483,900 T→A 5.5% V49D (GTC→GAC)  evgA → response regulator in two‑component regulatory system with EvgS
RA 2,483,912 T→A 5.2% V53D (GTC→GAC)  evgA → response regulator in two‑component regulatory system with EvgS
RA 2,519,222 T→G 6.4% intergenic (+17/+35) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
RA 2,519,225 T→A 5.4% intergenic (+20/+32) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
RA 2,519,228 C→A 5.7% intergenic (+23/+29) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
RA 2,654,142 T→A 6.3% noncoding (288/304 nt) ryfA → novel sRNA, function unknown
RA 2,654,143 T→A 6.2% noncoding (289/304 nt) ryfA → novel sRNA, function unknown
RA 2,654,144 T→A 5.7% noncoding (290/304 nt) ryfA → novel sRNA, function unknown
RA 2,768,609 T→C 5.6% intergenic (+36/‑56) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
RA 2,768,615 G→T 5.0% intergenic (+42/‑50) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
RA 2,768,620 G→A 5.0% intergenic (+47/‑45) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
RA 2,776,132 A→C 5.6% S72R (AGC→CGC)  yfjX → CP4‑57 prophage; putative antirestriction protein
RA 2,788,676 A→T 5.4% intergenic (+27/‑309) ygaQ → / → csiD uncharacterized protein; putative enzyme/carbon starvation protein
RA 2,962,964 T→G 5.2% T65P (ACG→CCG)  ygdB ← DUF2509 family protein
RA 3,067,320 A→T 7.6% intergenic (‑147/+20) ygfI ← / ← yggE putative DNA‑binding transcriptional regulator/oxidative stress defense protein
RA 3,067,323 A→T 8.1% intergenic (‑150/+17) ygfI ← / ← yggE putative DNA‑binding transcriptional regulator/oxidative stress defense protein
RA 3,107,876 A→C 5.5% I427S (ATT→AGT)  speC ← ornithine decarboxylase, constitutive
RA 3,250,989 G→C 5.6% intergenic (+35/‑35) yqjF → / → yqjG putative quinol oxidase subunit/putative S‑transferase
RA 3,250,991 A→T 5.2% intergenic (+37/‑33) yqjF → / → yqjG putative quinol oxidase subunit/putative S‑transferase
RA 3,251,392 C→A 10.3% A123A (GCC→GCA yqjG → putative S‑transferase
RA 3,266,132 C→A 5.2% G312C (GGT→TGT)  tdcA ← tdc operon transcriptional activator
RA 3,423,531 T→G 5.3% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,423,621 C→G 5.3% noncoding (35/76 nt) thrV ← tRNA‑Thr
RA 3,458,110 C→A 100% T578T (ACC→ACA gspD → general secretory pathway component, cryptic
RA 3,469,050 G→A 5.7% N268N (AAC→AAT chiA ← periplasmic endochitinase
RA 3,482,325 G→A 100% R346H (CGC→CAC)  yheS → ABC‑F family protein predicted regulatory ATPase
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,659,467 A→C 7.2% E79A (GAA→GCA) ‡ mdtE → anaerobic multidrug efflux transporter, ArcA‑regulated
RA 3,659,468 A→C 7.6% E79D (GAA→GAC) ‡ mdtE → anaerobic multidrug efflux transporter, ArcA‑regulated
RA 3,659,469 G→T 7.5% G80C (GGC→TGC) ‡ mdtE → anaerobic multidrug efflux transporter, ArcA‑regulated
RA 3,659,470 G→T 7.1% G80V (GGC→GTC) ‡ mdtE → anaerobic multidrug efflux transporter, ArcA‑regulated
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,966,862 G→A 24.2% R149H (CGT→CAT)  rho → transcription termination factor
RA 3,966,877 C→A 9.1% T154N (ACT→AAT)  rho → transcription termination factor
RA 3,967,036 T→A 6.3% L207Q (CTG→CAG)  rho → transcription termination factor
RA 3,967,449 C→T 19.4% L345F (CTC→TTC)  rho → transcription termination factor
RA 4,094,489 G→A 5.3% intergenic (‑217/+234) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,296,060 C→T 35.4% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,473,927 C→A 100% A43S (GCC→TCC)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,510,336 A→C 6.7% intergenic (+203/+354) yjhV → / ← fecE pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 448611 450722 2112 67 [64] [65] 66 [cyoC]–[cyoA] [cyoC],cyoB,[cyoA]
* * ÷ NC_000913 1166010 1167443 1434 67 [64] [65] 66 ndh ndh
* * ÷ NC_000913 3423685–3424234 3424569–3424238 5–885 68 [65] [64] 66 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 16653NA (NA)4 (0.030) 4/264 NT NA noncoding (1267/1345 nt) IS186 repeat region
?NC_000913 = 16691 NA (NA)noncoding (1305/1345 nt) IS186 repeat region
* ? NC_000913 34719 =NA (NA)3 (0.020) 3/272 NT NA intergenic (+24/+62) caiF/caiE cai operon transcriptional activator/stimulator of CaiD and CaiB enzyme activities
?NC_000913 34767 = NA (NA)intergenic (+72/+14) caiF/caiE cai operon transcriptional activator/stimulator of CaiD and CaiB enzyme activities
* ? NC_000913 274620 =NA (NA)6 (0.050) 3/260 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 = 274695NA (NA)3 (0.030) 3/270 NT NA noncoding (455/1195 nt) IS5 repeat region
?NC_000913 = 274727 NA (NA)noncoding (423/1195 nt) IS5 repeat region
* ? NC_000913 = 316201NA (NA)6 (0.050) 4/260 NT NA noncoding (973/1255 nt) IS3 repeat region
?NC_000913 = 316220 NA (NA)noncoding (992/1255 nt) IS3 repeat region
* ? NC_000913 = 382323NA (NA)3 (0.030) 3/268 NT NA noncoding (1064/1331 nt) IS2 repeat region
?NC_000913 = 382348 NA (NA)noncoding (1089/1331 nt) IS2 repeat region
* ? NC_000913 = 60104492 (0.750)7 (0.060) 6/260 NT 7.4% coding (2331/3144 nt) cusA copper/silver efflux system, membrane component
?NC_000913 = 601061 90 (0.780)coding (2348/3144 nt) cusA copper/silver efflux system, membrane component
* ? NC_000913 1207790 =29 (0.230)37 (0.340) 34/246 NT 51.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 45 (0.410)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780529 (0.230)60 (0.550) 49/246 NT 62.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 45 (0.410)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)131 (1.090) 93/270 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1468798 =NA (NA)3 (0.030) 3/264 NT NA noncoding (443/1331 nt) IS2 repeat region
?NC_000913 1468838 = NA (NA)noncoding (403/1331 nt) IS2 repeat region
* ? NC_000913 2729475 =70 (0.570)5 (0.040) 5/258 NT 8.3% intergenic (‑31/+141) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
?NC_000913 = 4168363 46 (0.400)intergenic (+163/‑9) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
* ? NC_000913 = 29475753 (0.020)5 (0.060)
+42 bp
5/194 NT 9.6% intergenic (+2/‑32) metW/metV tRNA‑Met/tRNA‑Met
?NC_000913 = 2947757 133 (1.080)coding (1254/1254 nt) amiC N‑acetylmuramoyl‑L‑alanine amidase
* ? NC_000913 = 3620350NA (NA)3 (0.030) 3/266 NT 100% coding (1159/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3620425 0 (0.000)coding (1234/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 4168332 =66 (0.530)5 (0.040) 3/258 NT 8.5% intergenic (+132/‑40) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
?NC_000913 4168374 = 46 (0.400)noncoding (3/76 nt) gltT tRNA‑Glu
* ? NC_000913 = 4394806110 (0.890)6 (0.050) 5/266 NT 5.3% coding (741/1548 nt) nnr bifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
?NC_000913 = 4394826 109 (0.920)coding (761/1548 nt) nnr bifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
* ? NC_000913 4542682 =105 (0.850)16 (0.140) 15/260 NT 13.4% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 109 (0.940)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 = 4542690102 (0.830)17 (0.150) 15/260 NT 14.3% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 109 (0.940)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)