breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,756,796 G→T E367* (GAA→TAA)  pykF → pyruvate kinase I
RA 1,757,387 C→G intergenic (+277/‑34) pykF → / → lpp pyruvate kinase I/murein lipoprotein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,312,590 G→A Q54* (CAG→TAG)  ompC ← outer membrane porin protein C
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
JC JC 3,311,380 IS4 (+) +12 bp noncoding (156‑167/174 nt) psrO → novel sRNA, function unknown
RA 3,413,571 T→C intergenic (‑106/‑293) acrS ← / → acrE acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,114,419 G→A A18A (GCC→GCT fpr ← ferredoxin‑NADP reductase; flavodoxin reductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423709–3424234 3424589–3424238 5–881 10 [2] [1] 3 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3781151 3781181 31 3 [1] [2] 3 lldD/trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
* * ÷ NC_000913 4136576 4136581 6 7 [2] [0] 6 yijE EamA‑like transporter family protein
* * ÷ NC_000913 4296120–4296378 4296397 20–278 4 [2] [2] 5 gltP/yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 225456 =NA (NA)3 (0.170) 3/114 1.7 NA noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 225477 = NA (NA)intergenic (+20/‑23) ileV/alaV tRNA‑Ile/tRNA‑Ala
* ? NC_000913 = 24300912 (0.600)4 (0.220) 3/118 1.8 24.5% coding (295/2445 nt) fadE acyl coenzyme A dehydrogenase
?NC_000913 = 243192 14 (0.790)coding (112/2445 nt) fadE acyl coenzyme A dehydrogenase
* ? NC_000913 315401 =NA (NA)7 (0.440) 6/106 0.8 NA noncoding (173/1255 nt) IS3 repeat region
?NC_000913 315627 = NA (NA)noncoding (399/1255 nt) IS3 repeat region
* ? NC_000913 = 381375NA (NA)4 (0.220) 3/118 1.8 NA noncoding (116/1331 nt) IS2 repeat region
?NC_000913 = 381732 NA (NA)noncoding (473/1331 nt) IS2 repeat region
* ? NC_000913 = 381480NA (NA)5 (0.260) 3/126 1.9 NA noncoding (221/1331 nt) IS2 repeat region
?NC_000913 = 381788 NA (NA)noncoding (529/1331 nt) IS2 repeat region
* ? NC_000913 = 382153NA (NA)6 (0.330) 3/120 1.8 NA noncoding (894/1331 nt) IS2 repeat region
?NC_000913 = 382246 NA (NA)noncoding (987/1331 nt) IS2 repeat region
* ? NC_000913 = 71556322 (1.100)5 (0.300) 4/112 1.4 24.0% coding (152/495 nt) ybfP lipoprotein
?NC_000913 = 715719 13 (0.770)coding (308/495 nt) ybfP lipoprotein
* ? NC_000913 726474 =11 (0.550)3 (0.160) 3/122 1.8 22.8% coding (563/2049 nt) kdpB potassium translocating ATPase, subunit B
?NC_000913 3621273 = NA (NA)coding (2082/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 857638 =NA (NA)13 (0.730) 5/118 1.2 36.1% noncoding (23/98 nt) RIP79 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP79 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 857687 = 23 (1.290)noncoding (72/98 nt) RIP79 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP79 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 = 99235314 (0.700)3 (0.170) 3/116 1.7 22.8% coding (1732/2613 nt) pepN aminopeptidase N
?NC_000913 = 992429 8 (0.460)coding (1808/2613 nt) pepN aminopeptidase N
* ? NC_000913 = 120780522 (1.100)5 (0.330) 5/100 1.0 26.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 11 (0.730)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)26 (1.390) 19/124 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1362001 =20 (1.000)6 (0.350) 4/114 1.4 28.4% coding (91/558 nt) puuR repressor for the divergent puu operons, putrescine inducible
?NC_000913 1362051 = 13 (0.760)coding (141/558 nt) puuR repressor for the divergent puu operons, putrescine inducible
* ? NC_000913 1683535 =20 (1.000)4 (0.230) 3/114 1.7 24.1% coding (654/1302 nt) rstB sensory histidine kinase of RstAB two‑component system
?NC_000913 1683597 = 8 (0.470)coding (716/1302 nt) rstB sensory histidine kinase of RstAB two‑component system
* ? NC_000913 = 179260016 (0.800)6 (0.340) 3/118 1.8 27.7% coding (132/465 nt) nlpC putative C40 clan peptidase lipoprotein
?NC_000913 = 1792651 17 (0.950)coding (81/465 nt) nlpC putative C40 clan peptidase lipoprotein
* ? NC_000913 2077808 =13 (0.650)5 (0.310) 4/108 1.3 20.2% coding (240/375 nt) cbtA CP4‑44 prophage; toxin of the YeeV‑YeeU toxin‑antitoxin system
?NC_000913 2077982 = 29 (1.780)pseudogene (43/168 nt) yeeW CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related
* ? NC_000913 = 307142414 (0.700)5 (0.290) 3/116 1.7 23.6% intergenic (‑180/+35) fbaA/pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
?NC_000913 = 3071467 20 (1.140)coding (1156/1164 nt) pgk phosphoglycerate kinase
* ? NC_000913 3091309 =11 (0.550)4 (0.220) 3/118 1.8 25.1% coding (176/732 nt) rsmE 16S rRNA m(3)U1498 methyltransferase, SAM‑dependent
?NC_000913 3091468 = 14 (0.790)coding (335/732 nt) rsmE 16S rRNA m(3)U1498 methyltransferase, SAM‑dependent
* ? NC_000913 = 340887012 (0.600)4 (0.230) 4/116 1.4 32.6% coding (1264/1452 nt) panF pantothenate:sodium symporter
?NC_000913 = 3408895 6 (0.340)coding (1289/1452 nt) panF pantothenate:sodium symporter
* ? NC_000913 = 361460417 (0.850)5 (0.280) 3/118 1.8 23.7% coding (938/1575 nt) nikA nickel/heme ABC transporter periplasmic binding protein
?NC_000913 = 3615035 17 (0.950)coding (1369/1575 nt) nikA nickel/heme ABC transporter periplasmic binding protein
* ? NC_000913 = 3621973NA (NA)4 (0.230) 3/114 1.7 NA coding (2782/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3622055 NA (NA)coding (2864/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 3625629 =NA (NA)4 (0.220) 4/120 1.5 40.0% noncoding (41/82 nt) RIP260 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP260 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 3625675 = 6 (0.330)intergenic (+161/+4) yhhI/yhhJ putative transposase/putative ABC transporter permease
* ? NC_000913 = 379832521 (1.050)8 (0.420) 4/126 1.6 26.7% coding (988/1074 nt) waaU lipopolysaccharide core biosynthesis
?NC_000913 = 3798516 24 (1.260)coding (797/1074 nt) waaU lipopolysaccharide core biosynthesis
* ? NC_000913 4341778 =16 (0.800)5 (0.350) 3/96 1.4 39.1% intergenic (‑150/‑133) melR/melA melibiose operon transcriptional regulator; autoregulator/alpha‑galactosidase, NAD(P)‑binding
?NC_000913 4341834 = 4 (0.280)intergenic (‑206/‑77) melR/melA melibiose operon transcriptional regulator; autoregulator/alpha‑galactosidase, NAD(P)‑binding