breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 8,332 C→T 5.8% A32V (GCC→GTC)  talB → transaldolase B
RA 194,942 Δ1 bp 8.7% coding (40/762 nt) ispU → undecaprenyl pyrophosphate synthase
RA 211,354 C→A 6.1% S559Y (TCT→TAT)  ldcC → lysine decarboxylase 2, constitutive
RA 222,958 Δ1 bp 100% coding (126/576 nt) gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase
RA 226,248 C→A 7.3% noncoding (490/2904 nt) rrlH → 23S ribosomal RNA of rrnH operon
RA 226,262 A→T 5.3% noncoding (504/2904 nt) rrlH → 23S ribosomal RNA of rrnH operon
RA 226,278 G→A 5.8% noncoding (520/2904 nt) rrlH → 23S ribosomal RNA of rrnH operon
RA 226,280 A→G 6.7% noncoding (522/2904 nt) rrlH → 23S ribosomal RNA of rrnH operon
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 344,938 G→T 6.0% G255C (GGT→TGT)  yahL → uncharacterized protein
RA 377,374 T→C 5.1% intergenic (+63/+161) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 459,667 G→T 11.1% A260A (GCG→GCT lon → DNA‑binding ATP‑dependent protease La
RA 459,751 G→T 12.7% A288A (GCG→GCT lon → DNA‑binding ATP‑dependent protease La
RA 513,920 C→A 5.6% intergenic (+52/‑73) ybaT → / → cueR putative amino acid transporter/copper‑responsive regulon transcriptional regulator
RA 566,173 C→G 15.3% pseudogene (204/279 nt) exoD ← pseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related
RA 566,205 T→C 17.3% pseudogene (172/279 nt) exoD ← pseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related
RA 566,245 G→A 17.4% pseudogene (132/279 nt) exoD ← pseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related
RA 566,323 C→T 8.9% pseudogene (54/279 nt) exoD ← pseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related
RA 566,326 T→C 8.9% pseudogene (51/279 nt) exoD ← pseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related
RA 566,332 T→G 8.8% pseudogene (45/279 nt) exoD ← pseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related
RA 590,608 C→A 5.9% R116L (CGA→CTA)  nfrA ← bacteriophage N4 receptor, outer membrane subunit
RA 699,468 A→C 6.5% intergenic (‑291/+106) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 702,583 C→T 5.1% D57N (GAT→AAT)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 845,660 C→A 5.6% intergenic (‑180/+81) ybiO ← / ← glnQ mechanosensitive channel protein, intermediate conductance/glutamine transporter subunit
RA 874,183 G→A 12.5% C402Y (TGT→TAT)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 961,268 A→T 9.1% K23M (AAG→ATG)  cmk → cytidylate kinase
RA 1,012,153 C→T 9.3% P51P (CCC→CCT pqiA → paraquat‑inducible, SoxRS‑regulated inner membrane protein
RA 1,155,411 G→A 6.9% V96V (GTG→GTA tmk → thymidylate kinase
RA 1,173,753 G→A 6.5% A71V (GCG→GTG)  mfd ← transcription‑repair coupling factor
RA 1,220,860 G→T 6.7% pseudogene (1260/2574 nt) ycgH → pseudogene; putative ATP‑binding component of a transport system
RA 1,270,419 T→C 8.6% noncoding (27/68 nt) rdlC → sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC
RA 1,287,187 A→T 7.5% noncoding (50/171 nt) rttR ← rtT sRNA, processed from tyrT transcript
RA 1,287,202 A→G 9.8% noncoding (35/171 nt) rttR ← rtT sRNA, processed from tyrT transcript
RA 1,328,032 C→A 5.8% W109L (TGG→TTG)  btuR ← cob(I)yrinic acid a,c‑diamide adenosyltransferase
RA 1,331,036 G→C 9.3% intergenic (‑368/‑12) yciN ← / → topA DUF2498 protein YciN/DNA topoisomerase I, omega subunit
RA 1,381,217 G→T 8.9% pseudogene (397/957 nt) ycjV → pseudogene; putative ATP‑binding component of a transport system
RA 1,405,543 C→A 8.7% P268Q (CCG→CAG)  abgR → putative DNA‑binding transcriptional regulator of abgABT operon
RA 1,428,541 A→G 5.5% R62R (CGT→CGC insH1 ← IS5 transposase and trans‑activator
RA 1,466,858 T→C 7.3% pseudogene (1467/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,478,386 C→A 7.6% S54* (TCA→TAA)  ynbB → putative CDP‑diglyceride synthase
RA 1,493,666 G→A 9.1% E399E (GAG→GAA trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
RA 1,527,358 A→G 6.0% intergenic (+206/‑544) yncH → / → rhsE IPR020099 family protein/pseudogene, Rhs family
RA 1,549,077 G→T 9.9% P559P (CCG→CCT fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,549,091 C→T 7.7% P564L (CCG→CTG)  fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,596,524 C→A 9.1% pseudogene (1464/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,670,751 C→T 6.4% I351I (ATC→ATT ynfM → putative arabinose efflux transporter
RA 1,790,389 G→T 13.9% Q286K (CAA→AAA)  ydiU ← UPF0061 family protein
RA 1,872,469 C→T 8.2% Y143Y (TAC→TAT yeaJ → putative diguanylate cyclase
RA 1,907,682 G→A 6.1% intergenic (‑91/+579) yobF ← / ← yebO DUF2527 family heat‑induced protein/putative inner membrane protein
RA 1,914,813 T→C 12.5% N2D (AAC→GAC)  prc ← carboxy‑terminal protease for penicillin‑binding protein 3
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 1,994,311 G→T 7.1% F132L (TTC→TTA uvrC ← excinuclease UvrABC, endonuclease subunit
RA 1,999,315 C→A 8.9% E56* (GAA→TAA)  dcyD ← D‑cysteine desulfhydrase, PLP‑dependent
RA 2,016,908 C→A 6.0% R119S (CGC→AGC)  fliI → flagellum‑specific ATP synthase
RA 2,019,349 G→A 6.1% G322S (GGC→AGC)  fliK → flagellar hook‑length control protein
RA 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein
RA 2,037,789 C→A 9.5% D122Y (GAT→TAT)  yedV ← putative sensory kinase in two‑component regulatory system with YedW
RA 2,046,314 G→A 10.8% L459L (CTG→CTA yeeJ → putative adhesin
RA 2,088,950 G→T 5.3% S60Y (TCT→TAT)  yeeZ ← putative epimerase
RA 2,089,011 C→A 10.5% A40S (GCC→TCC)  yeeZ ← putative epimerase
RA 2,128,645 C→A 7.5% D82Y (GAT→TAT)  wcaF ← putative acyl transferase
RA 2,158,616 C→T 7.4% V77V (GTC→GTT mdtC → multidrug efflux system, subunit C
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,192,944 C→A 8.0% intergenic (‑148/+115) yehE ← / ← mrp DUF2574 family protein/antiporter inner membrane protein
RA 2,358,043 C→A 7.5% *268L (TGA→TTA)  rhmA ← 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
JC JC 2,375,828 IS186 (+) +4 bp 100% coding (132‑135/963 nt) menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,581,618 G→A 7.5% V661V (GTG→GTA tktB → transketolase 2, thiamine triphosphate‑binding
RA 2,587,172 C→T 5.5% A481V (GCG→GTG)  narQ → sensory histidine kinase in two‑component regulatory system with NarP
RA 2,595,174 G→T 5.5% S229* (TCA→TAA)  tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase
RA 2,637,133 G→T 9.2% S75* (TCG→TAG)  der ← GTPase; multicopy suppressor of ftsJ
RA 2,767,845 G→A 7.3% G46R (GGG→AGG)  yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,776,388 G→T 8.5% M1M (ATG→ATT) † yfjY → CP4‑57 prophage; putative DNA repair protein
RA 2,778,170 T→C 14.6% V1519V (GTA→GTG ypjA ← adhesin‑like autotransporter
RA 2,818,046 A→G 12.9% intergenic (‑186/+13) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,818,049 T→A 7.3% intergenic (‑189/+10) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,830,202 G→T 6.0% L130L (CTG→CTT srlQ → D‑arabinose 5‑phosphate isomerase
RA 2,832,097 C→A 5.7% G65C (GGT→TGT)  norR ← anaerobic nitric oxide reductase DNA‑binding transcriptional activator
RA 2,843,836 G→T 5.1% L125L (CTC→CTA hycG ← hydrogenase 3 and formate hydrogenase complex, HycG subunit
RA 2,888,575 G→T 8.5% R509S (CGC→AGC)  cysI ← sulfite reductase, beta subunit, NAD(P)‑binding, heme‑binding
RA 2,904,212 G→A 100% intergenic (+838/+535) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 3,036,288 G→T 8.3% intergenic (‑6/+85) prfB ← / ← recJ peptide chain release factor RF‑2/ssDNA exonuclease, 5' ‑‑> 3'‑specific
RA 3,142,565 A→G 6.3% L142P (CTG→CCG)  hybC ← hydrogenase 2, large subunit
RA 3,173,553 C→A 6.1% R615L (CGC→CTC)  parE ← DNA topoisomerase IV, subunit B
RA 3,200,251 G→T 6.0% H239N (CAT→AAT)  ygiF ← inorganic triphosphatase
RA 3,258,115 G→T 6.8% intergenic (‑105/+170) yhaO ← / ← tdcG putative transporter/L‑serine dehydratase 3, anaerobic
RA 3,274,579 C→A 7.2% W110L (TGG→TTG)  garP ← putative (D)‑galactarate transporter
RA 3,365,487 C→T 5.1% pseudogene (303/367 nt) yhcE → pseudogene fragment; interrupted by IS5
RA 3,389,825 G→T 9.8% M102I (ATG→ATT aaeR → transcriptional regulator for aaeXAB operon
RA 3,420,607 G→T 5.7% V158V (GTG→GTT yhdX → putative amino acid ABC transporter permease
RA 3,423,531 T→G 8.7% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,440,923 C→A 100% G36V (GGC→GTC)  rpoA ← RNA polymerase, alpha subunit
RA 3,449,344 A→G 5.3% A180A (GCT→GCC rpsC ← 30S ribosomal subunit protein S3
RA 3,504,861 T→A 11.8% intergenic (+78/+74) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,504,867 T→A 16.4% intergenic (+84/+68) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,504,873 T→A 19.1% intergenic (+90/+62) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,504,875 T→A 15.5% intergenic (+92/+60) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,533,137 G→T 7.7% G107V (GGC→GTC)  pck → phosphoenolpyruvate carboxykinase [ATP]
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,593,141 C→A 5.3% E99D (GAG→GAT livF ← branched‑chain amino acid ABC transporter ATPase
JC JC 3,890,075 IS5 (–) +4 bp 100% coding (1346‑1349/1416 nt) tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,928,424 C→T 6.9% D394N (GAT→AAT)  viaA ← stimulator of RavA ATPase activity; von Willebrand factor domain protein
RA 3,936,090 T→A 5.4% L268Q (CTG→CAG)  rbsC → D‑ribose ABC transporter permease
RA 3,957,410 G→T 5.6% V137V (GTC→GTA ilvY ← transcriptional activator of ilvC; autorepressor
RA 3,957,432 G→T 5.1% A130E (GCA→GAA)  ilvY ← transcriptional activator of ilvC; autorepressor
RA 3,982,860 C→A 7.1% intergenic (+49/‑98) proM → / → aslB tRNA‑Pro/putative AslA‑specific sulfatase‑maturating enzyme
RA 4,003,800 G→T 8.8% R127R (CGG→AGG)  rarD ← putative chloramphenical resistance permease
RA 4,082,381 C→A 6.2% D127Y (GAC→TAC)  fdoH ← formate dehydrogenase‑O, Fe‑S subunit
RA 4,266,437 C→A 7.0% P219Q (CCG→CAG)  alr → alanine racemase, biosynthetic, PLP‑binding
RA 4,296,060 C→T 8.2% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,310,158 C→A 5.4% D275Y (GAC→TAC)  alsA ← D‑allose ABC transporter ATPase
RA 4,397,505 G→A 8.6% E32K (GAA→AAA)  mutL → methyl‑directed mismatch repair protein
RA 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
RA 4,433,483 A→G 6.0% V181A (GTG→GCG)  qorB ← NAD(P)H:quinone oxidoreductase
RA 4,484,773 A→G 5.6% T393T (ACT→ACC pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
RA 4,484,779 A→G 5.8% H391H (CAT→CAC pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
RA 4,518,816 A→G 6.7% pseudogene (96/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,545,735 G→T 9.5% G214W (GGG→TGG)  fimD → fimbrial usher outer membrane porin protein; FimCD chaperone‑usher
RA 4,559,943 C→A 6.9% G20C (GGC→TGC)  yjiG ← SpmB family inner membrane protein
RA 4,568,118 T→C 5.3% K134K (AAA→AAG mdtM ← multidrug efflux system protein
RA 4,633,743 C→T 100% M1M (ATG→ATA) † yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase
JC JC 4,640,551 IS1 (–) +9 bp 100% intergenic (+9/‑383) yjjY → / → yjtD uncharacterized protein/putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 773047 774161 1115 11 [0] [10] 11 cydB cytochrome d terminal oxidase, subunit II
* * ÷ NC_000913 1037724 1039292 1569 14 [9] [10] 11 cbdA cbdA
* * ÷ NC_000913 1166069 1167371 1303 11 [10] [0] 13 [ndh] [ndh]
* * ÷ NC_000913 3423782–3424234 3424518–3424238 5–737 12 [10] [9] 17 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 375000 =65 (1.110)8 (0.140) 3/230 NT 11.0% noncoding (71/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?NC_000913 377353 = NA (NA)noncoding (19/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? NC_000913 375202 =64 (1.160)6 (0.110) 4/218 NT 8.6% noncoding (273/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?NC_000913 377353 = NA (NA)noncoding (19/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? NC_000913 375219 =59 (1.040)5 (0.100) 4/196 NT 8.8% noncoding (290/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?NC_000913 377335 = NA (NA)noncoding (1/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? NC_000913 566098 =74 (1.140)6 (0.110)
+GAGAGGAGGATTACAAA
5/222 NT 7.6% pseudogene (279/279 nt) exoD pseudogene, DLP12 prophage; phage‑type exonuclease family;Phage or Prophage Related
?NC_000913 4397412 = 95 (1.460)coding (1/1848 nt) mutL methyl‑directed mismatch repair protein
* ? NC_000913 1207790 =11 (0.170)15 (0.260) 13/224 NT 57.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 13 (0.230)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078058 (0.120)24 (0.420) 13/224 NT 70.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 13 (0.230)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)37 (0.590) 22/248 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 246638488 (1.350)9 (0.160) 7/224 NT 13.6% intergenic (+1/‑161) argW/intS tRNA‑Arg/CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase
?NC_000913 2476600 = 37 (0.650)intergenic (+2/+94) pawZ/dsdC tRNA‑OTHER/dsd operon activator; autorepressor
* ? NC_000913 4373286 =43 (0.820)31 (0.590) 4/206 NT 41.9% noncoding (90/99 nt) RIP328 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP328 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 4374508 = NA (NA)noncoding (11/99 nt) RIP329 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP329 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site