mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | ||||||
|---|---|---|---|---|---|---|
| evidence | position | mutation | freq | annotation | gene | description |
| RA | 112,531 | A→C | 6.3% | intergenic (+209/‑95) | gltX → / → cysE | glutamate‑‑tRNA ligase/serine O‑acetyltransferase |
| RA | 132,152 | G→A | 73.3% | E549K (GAA→AAA) | fusA → | elongation factor G |
| RA | 132,185 | G→A | 20.0% | D560N (GAC→AAC) | fusA → | elongation factor G |
| RA | 135,117 | G→T | 11.0% | intergenic (+164/‑74) | EO946_RS00715 → / → rpsJ | alpha/beta hydrolase/30S ribosomal protein S10 |
| RA | 240,190 | G→T | 7.6% | F114L (TTC→TTA) | EO946_RS01360 ← | hypothetical protein |
| RA | 240,211 | G→T | 9.3% | A107A (GCC→GCA) | EO946_RS01360 ← | hypothetical protein |
| RA | 316,262 | C→A | 10.4% | Q28K (CAA→AAA) | opuAA → | glycine/proline betaine ABC transporter ATP‑binding protein OpuAA |
| RA | 376,966 | A→C | 5.2% | K781T (AAA→ACA) | srfAA → | surfactin non‑ribosomal peptide synthetase SrfAA |
| RA | 452,125 | T→A | 10.8% | K146I (AAA→ATA) | EO946_RS02320 ← | dienelactone hydrolase family protein |
| RA | 485,075 | C→A | 7.4% | H453N (CAC→AAC) | kimA → | potassium transporter KimA |
| RA | 549,106 | T→A | 6.3% | S40T (TCC→ACC) | EO946_RS02865 → | PLP‑dependent aminotransferase family protein |
| RA | 549,112 | A→C | 9.0% | R42R (AGA→CGA) | EO946_RS02865 → | PLP‑dependent aminotransferase family protein |
| RA | 607,359 | A→C | 12.7% | T68P (ACC→CCC) | EO946_RS03185 → | collagen‑like protein |
| RA | 607,413 | A→C | 9.4% | T86P (ACC→CCC) | EO946_RS03185 → | collagen‑like protein |
| RA | 607,501 | A→C | 7.2% | E115A (GAA→GCA) | EO946_RS03185 → | collagen‑like protein |
| RA | 607,554 | C→G | 6.8% | P133A (CCA→GCA) | EO946_RS03185 → | collagen‑like protein |
| RA | 607,628 | A→C | 33.1% | E157D (GAA→GAC) | EO946_RS03185 → | collagen‑like protein |
| RA | 607,630 | C→T | 23.4% | A158V (GCC→GTC) | EO946_RS03185 → | collagen‑like protein |
| RA | 607,674 | A→C | 9.0% | T173P (ACC→CCC) | EO946_RS03185 → | collagen‑like protein |
| RA | 607,699 | A→C | 8.4% | E181A (GAA→GCA) ‡ | EO946_RS03185 → | collagen‑like protein |
| RA | 607,700 | A→C | 8.6% | E181D (GAA→GAC) ‡ | EO946_RS03185 → | collagen‑like protein |
| RA | 607,783 | T→C | 6.0% | V209A (GTC→GCC) | EO946_RS03185 → | collagen‑like protein |
| RA | 607,838 | A→G | 15.4% | T227T (ACA→ACG) | EO946_RS03185 → | collagen‑like protein |
| RA | 767,718 | T→G | 7.4% | S83R (AGC→CGC) | EO946_RS03930 ← | hypothetical protein |
| RA | 767,723 | A→C | 8.8% | I81S (ATT→AGT) | EO946_RS03930 ← | hypothetical protein |
| RA | 845,015 | T→A | 5.4% | L316L (CTA→CTT) | yfkAB ← | radical SAM/CxCxxxxC motif protein YfkAB |
| RA | 845,024 | T→G | 5.5% | T313T (ACA→ACC) | yfkAB ← | radical SAM/CxCxxxxC motif protein YfkAB |
| RA | 845,030 | G→A | 5.3% | G311G (GGC→GGT) | yfkAB ← | radical SAM/CxCxxxxC motif protein YfkAB |
| RA | 845,033 | A→G | 5.1% | F310F (TTT→TTC) | yfkAB ← | radical SAM/CxCxxxxC motif protein YfkAB |
| RA | 1,014,009 | G→T | 8.1% | R56R (CGC→CGA) | EO946_RS05230 ← | polysaccharide deacetylase |
| RA | 1,112,969 | C→T | 10.2% | L1022F (CTT→TTT) | addB → | helicase‑exonuclease AddAB subunit AddB |
| RA | 1,176,173 | G→T | 10.8% | G135V (GGT→GTT) | med → | transcriptional regulator Med |
| RA | 1,366,028 | A→C | 7.0% | T176P (ACG→CCG) | EO946_RS07135 → | PepSY domain‑containing protein |
| RA | 2,056,112 | G→T | 5.0% | intergenic (‑208/+32) | EO946_RS10315 ← / ← EO946_RS10320 | CPBP family intramembrane metalloprotease/glutamine amidotransferase |
| RA | 2,073,387 | A→T | 5.3% | G302G (GGT→GGA) | EO946_RS10385 ← | NTTRR‑F1 domain |
| RA | 2,103,816 | C→A | 8.5% | intergenic (‑33/+118) | azoJ ← / ← EO946_RS10560 | FMN‑dependent NADH‑azoreductase AzoJ/TraR/DksA family transcriptional regulator |
| RA | 2,139,480 | A→C | 8.0% | A119A (GCT→GCG) | deoD ← | purine‑nucleoside phosphorylase |
| RA | 2,387,114 | C→A | 6.8% | V338L (GTA→TTA) | EO946_RS12195 ← | acetyl‑CoA C‑acetyltransferase |
| RA | 2,644,019 | A→C | 6.5% | V322V (GTT→GTG) | ruvB ← | Holliday junction branch migration DNA helicase RuvB |
| RA | 2,649,600 | T→C | 6.6% | intergenic (+3/+43) | EO946_RS13705 → / ← EO946_RS13710 | hypothetical protein/hypothetical protein |
| RA | 2,649,964 | C→A | 6.0% | intergenic (‑199/‑75) | EO946_RS13710 ← / → EO946_RS13715 | hypothetical protein/alanine:cation symporter family protein |
| RA | 2,680,491 | A→C | 8.4% | F387V (TTC→GTC) | EO946_RS13875 ← | LysM peptidoglycan‑binding domain‑containing protein |
| RA | 2,979,248 | T→C | 26.1% | noncoding (1553/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
| RA | 2,980,160 | G→A | 25.0% | noncoding (641/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
| RA | 2,980,283 | T→G | 18.7% | noncoding (518/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
| RA | 2,982,148 | G→T | 18.2% | noncoding (372/1550 nt) | EO946_RS15435 ← | 16S ribosomal RNA |
| RA | 2,991,252 | C→A | 26.2% | intergenic (+53/‑154) | gbsR → / → bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
| RA | 3,184,726 | A→C | 5.3% | intergenic (+22/+15) | mdtP → / ← EO946_RS16485 | multidrug efflux MFS transporter MdtP/tautomerase family protein |
| RA | 3,184,761 | T→G | 5.4% | R122R (AGG→CGG) | EO946_RS16485 ← | tautomerase family protein |
| RA | 3,200,979 | G→T | 7.3% | G200* (GGA→TGA) | EO946_RS16570 → | spore germination protein |
| RA | 3,201,057 | C→G | 6.1% | L226V (CTG→GTG) | EO946_RS16570 → | spore germination protein |
| RA | 3,247,065 | C→T | 100% | intergenic (‑11/+110) | bdbD ← / ← cadA | disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase |
| RA | 3,268,712 | G→T | 5.2% | intergenic (+60/+150) | catR → / ← EO946_RS16920 | catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase |
| RA | 3,280,377 | G→T | 9.2% | Q115K (CAA→AAA) | EO946_RS16965 ← | lantibiotic dehydratase |
| RA | 3,285,567 | C→T | 37.8% | L278L (CTG→CTA) | EO946_RS16995 ← | IS3 family transposase |
| RA | 3,285,575 | T→G | 36.6% | N276H (AAT→CAT) | EO946_RS16995 ← | IS3 family transposase |
| RA | 3,285,580 | A→G | 40.4% | I274T (ATA→ACA) | EO946_RS16995 ← | IS3 family transposase |
| RA | 3,285,647 | C→A | 42.2% | V252L (GTA→TTA) | EO946_RS16995 ← | IS3 family transposase |
| RA | 3,285,713 | G→A | 33.2% | L230L (CTA→TTA) | EO946_RS16995 ← | IS3 family transposase |
| RA | 3,294,893 | T→G | 5.9% | N293H (AAC→CAC) | eno ← | phosphopyruvate hydratase |
| RA | 3,447,038 | T→G | 9.8% | N183T (AAC→ACC) | degU ← | two‑component system response regulator DegU |
| RA | 3,496,459 | C→A | 5.8% | G323G (GGG→GGT) | EO946_RS17995 ← | glucosaminidase domain‑containing protein |
| RA | 3,637,344 | T→A | 14.7% | Y44N (TAC→AAC) | EO946_RS18740 → | DUF2529 domain‑containing protein |
| RA | 3,851,230 | G→T | 7.8% | Q49K (CAG→AAG) | EO946_RS19820 ← | hypothetical protein |
| RA | 3,851,237 | G→T | 10.4% | I46I (ATC→ATA) | EO946_RS19820 ← | hypothetical protein |
| RA | 3,976,114 | G→T | 6.4% | A31E (GCA→GAA) | rlmH ← | 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH |
| RA | 3,976,149 | G→T | 8.1% | G19G (GGC→GGA) | rlmH ← | 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH |
| RA | 3,992,777 | G→T | 81.5% | L295M (CTG→ATG) | walK ← | cell wall metabolism sensor histidine kinase WalK |
| RA | 3,996,838 | G→T | 7.3% | intergenic (‑144/+62) | purA ← / ← EO946_RS20530 | adenylosuccinate synthase/VOC family protein |
| RA | 3,996,857 | C→A | 9.1% | intergenic (‑163/+43) | purA ← / ← EO946_RS20530 | adenylosuccinate synthase/VOC family protein |
| RA | 4,022,597 | G→T | 5.3% | V607F (GTT→TTT) | EO946_RS20675 → | thioredoxin domain‑containing protein |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NZ_CP034943 | 607623 = | 109 (0.430) | 40 (0.170) +AGTAGCCCCT |
20/232 | NT | 17.3% | coding (466/1125 nt) | EO946_RS03185 | collagen‑like protein |
| ? | NZ_CP034943 | = 607846 | 307 (1.200) | coding (689/1125 nt) | EO946_RS03185 | collagen‑like protein | |||||