breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 5,525 C→T R98C (CGC→TGC)  yaaX → DUF2502 domain‑containing protein YaaX
RA 7,485 T→C S159G (AGC→GGC)  yaaJ ← putative transporter YaaJ
RA 60,693 A→G F858L (TTT→CTT)  rapA ← RNA polymerase‑binding ATPase and RNAP recycling factor
RA 110,484 C→T R736C (CGT→TGT)  secA → protein translocation ATPase
RA 112,822 C→T S133N (AGC→AAC)  coaE ← dephospho‑CoA kinase
RA 126,095 A→G E134G (GAA→GGA)  aceF → pyruvate dehydrogenase, E2 subunit
RA 135,603 A→G S288P (TCC→CCC)  speE ← spermidine synthase
RA 178,897 A→G V86A (GTG→GCG)  mtn ← 5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
RA 187,027 T→C N542D (AAC→GAC)  glnD ← PII uridylyltransferase/uridylyl removing enzyme
RA 232,233 G→A G103D (GGT→GAT)  yafE → putative S‑adenosylmethionine‑dependent methyltransferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 308,798 C→T V179I (GTC→ATC)  ecpC ← putative fimbrial usher protein EcpC
RA 346,213 A→G V42A (GTA→GCA)  yahN ← putative amino acid exporter
RA 378,007 A→G I121T (ATC→ACC)  frmB ← S‑formylglutathione hydrolase FrmB
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 442,297 A→G E250G (GAA→GGA)  thiI → tRNA uridine 4‑sulfurtransferase
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 626,907 C→T P280S (CCA→TCA)  entE → 2,3‑dihydroxybenzoate‑AMP ligase
RA 692,847 C→T G178S (GGT→AGT)  ybeZ ← PhoH‑like protein
RA 693,757 A→G L400P (CTT→CCT)  miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 696,480 T→C noncoding (25/75 nt) glnX ← tRNA‑Gln
RA 705,972 (T)9→8 intergenic (+82/‑121) nagE → / → glnS N‑acetylglucosamine specific PTS enzyme IIABC component/glutamine‑‑tRNA ligase
RA 727,833 C→T W300* (TGG→TGA kdpA ← K(+) transporting P‑type ATPase subunit KdpA
RA 740,236 A→G T244A (ACG→GCG)  phr → deoxyribodipyrimidine photolyase (photoreactivation)
RA 741,817 C→T G247D (GGC→GAC)  dtpD ← dipeptide:H(+) symporter DtpD
RA 746,405 A→G L157L (TTA→TTG nei → endonuclease VIII
RA 761,926 C→T T135T (ACC→ACT sucB → dihydrolipoyltranssuccinylase
RA 773,688 C→T A216V (GCA→GTA)  cydB → cytochrome bd‑I ubiquinol oxidase subunit II
RA 868,422 (C)8→7 coding (903/1872 nt) gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 869,757 C→T A116V (GCC→GTC)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 873,432 G→A A152T (GCT→ACT)  pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 879,588 (T)6→7 coding (847/1116 nt) yliI → aldose sugar dehydrogenase YliI
RA 886,986 A→G G118G (GGT→GGC ybjJ ← inner membrane protein YbjJ
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,046,011 (A)5→4 coding (1935/2097 nt) gfcD ← putative lipoprotein GfcD
RA 1,058,611 T→C G176G (GGT→GGC torC → cytochrome c menaquinol dehydrogenase TorC
RA 1,103,757 A→G intergenic (‑561/‑194) csgD ← / → csgB DNA‑binding transcriptional dual regulator CsgD/curlin, minor subunit
RA 1,109,564 C→T P77L (CCA→CTA)  opgG → osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
RA 1,128,274 C→T L146L (CTG→TTG)  murJ → putative lipid II flippase MurJ
RA 1,132,112 A→G S87G (AGC→GGC)  flgD → flagellar biosynthesis, initiation of hook assembly
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,165,892 (T)6→7 intergenic (+207/‑193) ycfP → / → ndh UPF0227 protein YcfP/NADH:quinone oxidoreductase II
RA 1,167,058 (G)5→7 coding (974/1305 nt) ndh → NADH:quinone oxidoreductase II
RA 1,181,216 C→T A18T (GCA→ACA)  ycfZ ← putative inner membrane protein
RA 1,190,062 A→G L129P (CTC→CCC)  phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,204,860 A→G T231A (ACC→GCC) 
pseudogene (460/1137 nt)
ymfN →
aaaE →
e14 prophage; chimeric replication protein/phage terminase YmfN
phage terminase
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,241,020 A→G V351A (GTG→GCG)  cvrA ← putative K(+):H(+) antiporter
RA 1,260,895 A→C intergenic (‑92/+33) dauA ← / ← prs aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase
RA 1,261,174 T→C E234E (GAA→GAG prs ← ribose‑phosphate diphosphokinase
RA 1,286,729 A→G L7L (TTA→TTG ychS → putative uncharacterized protein YchS
RA 1,306,353 T→C V197A (GTG→GCG)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,351,196 (G)7→6 intergenic (‑157/+211) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
RA 1,409,811 A→G T101A (ACC→GCC)  dbpA → ATP‑dependent RNA helicase DbpA
RA 1,431,864 2 bp→TT coding (2816‑2817/3363 nt) stfR → Rac prophage; putative membrane protein
RA 1,450,766 G→A A195V (GCG→GTG)  tynA ← copper‑containing amine oxidase
RA 1,458,126 C→T R210R (CGC→CGT paaF → putative 2,3‑dehydroadipyl‑CoA hydratase
RA 1,465,714 A→G pseudogene (323/2518 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,494,083 T→C G538G (GGT→GGC trg → methyl‑accepting chemotaxis protein Trg
RA 1,495,469 C→T A61V (GCG→GTG)  ydcJ → DUF1338 domain‑containing protein YdcJ
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,541,687 T→C T302A (ACT→GCT)  narZ ← nitrate reductase Z subunit alpha
RA 1,554,860 G→A D270D (GAC→GAT maeA ← malate dehydrogenase, NAD‑requiring
RA 1,565,239 A→G F165F (TTT→TTC dosP ← oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
RA 1,594,248 T→C pseudogene (3839/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,666,566 A→G S418G (AGC→GGC) 
A218A (GCT→GCC
clcB →
ynfK ←
putative chloride:H(+) antiporter ClcB
putative dethiobiotin synthetase
RA 1,669,419 C→T L58L (TTG→TTA ynfL ← putative DNA‑binding transcriptional regulator
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
RA 1,717,884 C→T A42T (GCT→ACT)  pdxH ← pyridoxal 5‑phosphate synthase
RA 1,728,630 C→T A95V (GCG→GTG)  rnt → RNase T
RA 1,758,128 T→C S200G (AGC→GGC)  ldtE ← L,D‑transpeptidase LdtE
RA 1,769,478 (G)7→8 coding (405/1113 nt) ydiK → putative transporter YdiK
RA 1,794,316 G→A C279C (TGC→TGT btuC ← vitamin B12 ABC transporter membrane subunit
RA 1,809,491 (G)5→6 coding (112/669 nt) hxpB → hexitol phosphatase B
RA 1,818,550 (C)7→9 intergenic (‑50/+55) chbF ← / ← chbR monoacetylchitobiose‑6‑phosphate hydrolase/DNA‑binding transcriptional dual regulator ChbR
RA 1,860,673 T→C E220E (GAA→GAG ydjL ← putative zinc‑binding dehydrogenase YdjL
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,894,349 (A)8→9 coding (217/300 nt) yoaC → DUF1889 domain‑containing protein YoaC
RA 1,910,008 Δ1 bp coding (92/792 nt) kdgR ← DNA‑binding transcriptional repressor KdgR
RA 1,940,023 T→C R57R (CGA→CGG lpxM ← myristoyl‑acyl carrier protein‑dependent acyltransferase
RA 1,940,663 T→C T325A (ACG→GCG)  mepM ← peptidoglycan DD‑endopeptidase MepM
RA 1,957,484 T→C T217A (ACC→GCC)  torY ← cytochrome c quinol dehydrogenase TorY
RA 1,962,324 T→C V118A (GTT→GCT)  yecT → protein YecT
RA 1,967,136 G→A A101V (GCG→GTG)  cheY ← chemotaxis protein CheY
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,068,622 A→G intergenic (‑1301/‑13) insH‑6 ← / → yoeA CP4‑44 prophage; IS5 transposase and trans‑activator/CP4‑44 prophage; TonB‑dependent receptor plug domain‑containing protein YoeA
RA 2,095,848 T→C W242R (TGG→CGG) 
L2P (CTG→CCG) 
hisA →
hisF →
1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
imidazole glycerol phosphate synthase subunit HisF
RA 2,099,367 T→C T83A (ACC→GCC)  ugd ← UDP‑glucose 6‑dehydrogenase
RA 2,160,491 G→A A702A (GCG→GCA mdtC → multidrug efflux pump RND permease subunit MdtC
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,225,211 Δ1 bp coding (421/588 nt) yohC ← putative inner membrane protein
RA 2,235,140 A→G T370A (ACC→GCC)  preT → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
RA 2,241,486 G→A T61T (ACC→ACT galS ← DNA‑binding transcriptional dual regulator GalS
RA 2,338,351 T→C H357R (CAC→CGC)  gyrA ← DNA gyrase subunit A
RA 2,339,161 T→C D87G (GAC→GGC)  gyrA ← DNA gyrase subunit A
RA 2,344,855 T→C intergenic (‑118/‑10) ypaB ← / → nrdA protein YpaB/ribonucleoside‑diphosphate reductase 1 subunit alpha
RA 2,363,540 G→A H32Y (CAT→TAT)  yfaY ← IPR008135 CinA family protein YfaY
RA 2,445,720 T→C E222G (GAG→GGG)  mepA ← peptidoglycan DD‑endopeptidase/peptidoglycan LD‑endopeptidase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,448,886 C→T P94L (CCG→CTG)  smrB → putative endonuclease SmrB
RA 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,514,208 T→C S389S (AGT→AGC nupC → nucleoside:H(+) symporter NupC
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,579,484 T→C A273A (GCT→GCC talA → transaldolase A
RA 2,627,231 C→T A570T (GCT→ACT)  pdeF ← cyclic di‑GMP phosphodiesterase PdeF
RA 2,750,687 T→C T8A (ACG→GCG)  grpE ← nucleotide exchange factor GrpE
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,826,281 T→C G150G (GGT→GGC srlA → sorbitol‑specific PTS enzyme IIC2 component
RA 2,849,765 G→A H29Y (CAC→TAC)  hycB ← formate hydrogenlyase subunit HycB
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,884,854 T→A D789V (GAT→GTT)  cas3 ← CRISPR‑associated endonuclease/helicase Cas3
RA 2,945,208 T→C Y212C (TAC→TGC)  tcdA ← tRNA threonylcarbamoyladenosine dehydratase
RA 2,946,849 G→A A110A (GCC→GCT mltA ← membrane‑bound lytic murein transglycosylase A
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,981,582 T→C S201G (AGC→GGC)  araE ← arabinose:H(+) symporter
RA 2,988,052 G→A A173T (GCT→ACT)  yqeH → putative LuxR family transcriptional regulator YqeH
RA 3,001,304 (G)7→8 coding (960/2259 nt) xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,093,323 T→C G129G (GGT→GGC yqgE → DUF179 domain‑containing protein YqgE
RA 3,100,556 A→G V58A (GTT→GCT)  ansB ← L‑asparaginase 2
RA 3,101,039 (C)7→8 coding (585/720 nt) yggN ← putative EcfF
RA 3,123,348 G→A A153V (GCG→GTG)  glcB ← malate synthase G
RA 3,129,750 T→C T156A (ACA→GCA)  yghO ← putative DNA‑binding transcriptional regulator YghO
RA 3,208,832 G→A R270H (CGC→CAC)  ttdT → L‑tartrate:succinate antiporter
RA 3,247,457 G→A intergenic (+29/‑316) exuR → / → yqjA DNA‑binding transcriptional repressor ExuR/DedA family protein YqjA
RA 3,269,956 T→C intergenic (+354/+260) yhaC → / ← rnpB uncharacterized protein YhaC/RNase P catalytic RNA component
RA 3,311,564 C→T G41S (GGC→AGC)  rpsO ← 30S ribosomal subunit protein S15
RA 3,327,342 G→A A108V (GCA→GTA)  rlmE ← 23S rRNA 2'‑O‑ribose U2552 methyltransferase
RA 3,350,184 (C)7→8 coding (276/654 nt) elbB ← low activity glyoxalase ElbB
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,471,380 T→C intergenic (‑51/+20) tufA ← / ← fusA translation elongation factor Tu 1/elongation factor G
RA 3,483,399 T→C P66P (CCT→CCC yheT → putative hydrolase YheT
RA 3,495,499 T→C F497L (TTC→CTC)  nirB → nitrite reductase catalytic subunit NirB
RA 3,546,924 G→A L122L (CTG→CTA gntT → high‑affinity gluconate transporter
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,590,504 C→T L136L (CTG→CTA ugpA ← sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA
RA 3,610,787 G→A A316V (GCC→GTC)  yhhS ← putative transporter YhhS
RA 3,612,522 (G)5→6 coding (658/1050 nt) yhhT → putative transporter YhhT
RA 3,708,172 (A)7→8 intergenic (‑467/+444) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,728,118 (C)8→9 coding (1254/1455 nt) xylB ← xylulokinase
RA 3,795,971 C→T A348V (GCC→GTC)  waaF → ADP‑heptose‑‑LPS heptosyltransferase 2
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,819,902 T→C K423K (AAA→AAG ligB ← DNA ligase B
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,988,542 C→T P175P (CCG→CCA hemX ← PF04375 family protein HemX
RA 4,020,399 A→G R58R (CGA→CGG ubiB → ubiquinone biosynthesis protein UbiB
RA 4,037,157 T→C noncoding (17/77 nt) ileT → tRNA‑Ile
RA 4,060,333 A→G intergenic (+103/‑114) bipA → / → yihL ribosome‑dependent GTPase, ribosome assembly factor/putative transcriptional regulator YihL
RA 4,073,963 A→G E75E (GAA→GAG yihV → 6‑deoxy‑6‑sulfofructose kinase
RA 4,078,054 (A)5→4 coding (606/990 nt) yiiD → putative acetyltransferase YiiD
MC JC 4,180,672 (CTG)4→3 coding (113‑115/366 nt) rplL → 50S ribosomal subunit protein L12
RA 4,193,779 T→C A142A (GCA→GCG thiE ← thiamine phosphate synthase
RA 4,225,841 A→G T672A (ACC→GCC)  metH → cobalamin‑dependent methionine synthase
RA 4,227,040 A→G A1071A (GCA→GCG metH → cobalamin‑dependent methionine synthase
RA 4,287,910 T→C H49H (CAT→CAC nrfA → cytochrome c552 nitrite reductase
RA 4,291,056 C→T A194V (GCG→GTG)  nrfD → putative menaquinol‑cytochrome c reductase subunit NrfD
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,309,833 A→G V383A (GTT→GCT)  alsA ← D‑allose ABC transporter ATP binding subunit
RA 4,365,583 A→G G397G (GGT→GGC dcuA ← C4‑dicarboxylate transporter DcuA
RA 4,375,232 A→G R142R (CGT→CGC epmB ← lysine 2,3‑aminomutase
RA 4,403,541 C→T T81I (ACC→ATC)  hflC → regulator of FtsH protease
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,465,733 A→G Y150H (TAC→CAC)  treB ← trehalose‑specific PTS enzyme IIBC component
RA 4,469,600 A→G D659G (GAC→GGC)  mgtA → Mg(2(+)) importing P‑type ATPase
RA 4,557,577 G→A A238V (GCC→GTC)  hypT ← DNA‑binding transcriptional dual regulator HypT

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1432379–1432653 1434251–1433375 723–1873 36 [34] [34] 38 [stfR]–ynaE [stfR],tfaR,pinR,ynaE
* * ÷ NC_000913 3423759–3424550 3424550 1–792 36 [33] [33] 35 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)103 (0.940) 63/268 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =186 (1.650)183 (1.630) 89/274 0.0 66.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)132 (1.170) 75/276 0.1 98.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =94 (0.830)39 (0.370) 31/258 2.1 30.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 93 (0.880)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269094 (0.830)33 (0.310) 25/258 2.9 26.7% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 93 (0.880)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit