breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA CP000731 81 N→G 100% intergenic (–/‑151)  / → repA –/replication protein A
JC CP000731 6,552 +28 bp 100% intergenic (+1069/‑419) cadX → / → USA300HOU_pUSA300HOUMR0010 cadmium efflux regulator/MarR family transcriptional regulator
RA CP000731 25,663 Δ1 bp 100% coding (316/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein
RA USA300TCH1516_ALE 110,651 C→T 8.3% D170D (GAC→GAT USA300HOU_RS00515 → putative lipoprotein
RA USA300TCH1516_ALE 110,660 T→A 13.8% I173I (ATT→ATA USA300HOU_RS00515 → putative lipoprotein
RA USA300TCH1516_ALE 111,512 A→G 31.1% S194S (TCA→TCG USA300HOU_RS00520 → putative lipoprotein
RA USA300TCH1516_ALE 111,516 C→A 30.1% Q196K (CAA→AAA)  USA300HOU_RS00520 → putative lipoprotein
RA USA300TCH1516_ALE 111,560 T→C 7.0% R210R (CGT→CGC USA300HOU_RS00520 → putative lipoprotein
RA USA300TCH1516_ALE 128,994 C→T 6.0% L198L (TTG→TTA spa ← Immunoglobulin G‑binding protein A
RA USA300TCH1516_ALE 151,520 C→A 5.3% S117R (AGC→AGA USA300HOU_RS00690 → hypothetical protein
RA USA300TCH1516_ALE 456,608 A→T 6.7% E55D (GAA→GAT tst_4 → Toxic shock syndrome toxin‑1
RA USA300TCH1516_ALE 486,180 G→T 5.3% intergenic (+56/‑161) USA300TCH1516_00437 → / → mccA hypothetical protein/O‑acetylserine dependent cystathionine beta‑synthase
RA USA300TCH1516_ALE 512,643 A→G 47.0% intergenic (+711/‑6052) recR → / → speA_2 Recombination protein RecR/Arginine decarboxylase
JC USA300TCH1516_ALE 514,457 Δ16 bp 60.4% intergenic (+2525/‑4223) recR → / → speA_2 Recombination protein RecR/Arginine decarboxylase
RA USA300TCH1516_ALE 614,094 T→C 5.5% S802S (AGT→AGC sdrC → Serine‑aspartate repeat‑containing protein C
RA USA300TCH1516_ALE 614,172 T→C 5.1% S828S (AGT→AGC sdrC → Serine‑aspartate repeat‑containing protein C
RA USA300TCH1516_ALE 618,351 T→C 7.7% D1151D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 618,387 T→C 6.0% D1163D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 618,489 T→C 7.3% D1197D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 618,513 T→C 5.4% D1205D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 618,567 T→C 5.3% D1223D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 618,621 T→C 5.1% D1241D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 618,636 G→A 6.8% S1246S (TCG→TCA sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 618,657 T→C 6.9% D1253D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 618,666 T→C 5.3% S1256S (AGT→AGC sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 618,693 T→C 6.8% D1265D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 618,714 G→A 6.4% S1272S (TCG→TCA sdrD → Serine‑aspartate repeat‑containing protein D
RA USA300TCH1516_ALE 622,359 T→C 6.8% D974D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E
RA USA300TCH1516_ALE 622,464 G→A 9.5% S1009S (TCG→TCA sdrE → Serine‑aspartate repeat‑containing protein E
RA USA300TCH1516_ALE 622,479 T→C 5.1% D1014D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E
RA USA300TCH1516_ALE 622,521 T→C 5.8% D1028D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E
RA USA300TCH1516_ALE 622,533 T→C 6.0% D1032D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E
RA USA300TCH1516_ALE 622,536 G→A 6.7% S1033S (TCG→TCA sdrE → Serine‑aspartate repeat‑containing protein E
RA USA300TCH1516_ALE 776,295 G→T 5.2% intergenic (+97/‑124) nagA → / → corC N‑acetylglucosamine‑6‑phosphate deacetylase/Magnesium and cobalt efflux protein CorC
RA USA300TCH1516_ALE 1,141,234 T→A 6.6% S285T (TCA→ACA)  USA300HOU_RS05655 → hypothetical protein
RA USA300TCH1516_ALE 1,333,789 T→G 6.6% Y64D (TAT→GAT)  hflX → GTPase HflX
RA USA300TCH1516_ALE 1,370,154 C→A 5.4% A15D (GCT→GAT)  USA300TCH1516_01280 → hypothetical protein
RA USA300TCH1516_ALE 1,416,458 A→T 100% I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD
RA USA300TCH1516_ALE 1,435,065 C→G 10.3% A141G (GCT→GGT)  dapH → 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
RA USA300TCH1516_ALE 1,435,073 G→A 10.0% G144S (GGT→AGT) ‡ dapH → 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
RA USA300TCH1516_ALE 1,435,075 T→A 6.7% G144G (GGT→GGA) ‡ dapH → 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
RA USA300TCH1516_ALE 1,435,081 T→A 8.7% N146K (AAT→AAA dapH → 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑acetyltransferase
RA USA300TCH1516_ALE 1,472,936 A→T 6.4% A9696A (GCT→GCA ebh_1 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 1,473,069 C→T 5.4% G9652D (GGC→GAC)  ebh_1 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_RS07375 → hypothetical protein
RA USA300TCH1516_ALE 1,646,189 G→C 100% A155G (GCA→GGA)  ypdF ← Aminopeptidase YpdF
RA USA300TCH1516_ALE 1,744,428 T→C 7.5% K82E (AAA→GAA)  apt ← Adenine phosphoribosyltransferase
RA USA300TCH1516_ALE 1,857,785 A→T 12.2% S197T (TCA→ACA)  USA300HOU_RS09235 ← hypothetical protein
RA USA300TCH1516_ALE 1,857,867 C→T 12.0% L169L (TTG→TTA USA300HOU_RS09235 ← hypothetical protein
RA USA300TCH1516_ALE 1,857,944 A→T 8.6% S144T (TCA→ACA)  USA300HOU_RS09235 ← hypothetical protein
RA USA300TCH1516_ALE 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_RS10125 ← Putative multidrug export ATP‑binding/permease protein
RA USA300TCH1516_ALE 2,018,582 T→A 6.9% K113* (AAA→TAA)  mgt ← Monofunctional glycosyltransferase
RA USA300TCH1516_ALE 2,018,819 G→T 5.6% P34T (CCG→ACG)  mgt ← Monofunctional glycosyltransferase
RA USA300TCH1516_ALE 2,028,124 G→A 69.7% P174L (CCG→CTG)  USA300HOU_RS10230 ← hypothetical protein
RA USA300TCH1516_ALE 2,028,652 C→T 9.6% intergenic (‑8/+7) USA300HOU_RS10230 ← / ← USA300HOU_RS10235 hypothetical protein/hypothetical protein
RA USA300TCH1516_ALE 2,124,510 C→A 7.3% S213* (TCA→TAA)  lexA_2 → LexA repressor
RA USA300TCH1516_ALE 2,124,529 C→A 8.0% D219E (GAC→GAA lexA_2 → LexA repressor
RA USA300TCH1516_ALE 2,211,305 A→G 5.5% intergenic (+298/+515) USA300HOU_RS11275 → / ← yidC hypothetical protein/Membrane protein insertase YidC
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,281,993 A→T 7.4% N180K (AAT→AAA ebh_3 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,358,670 T→C 6.7% K83R (AAA→AGA)  rpoA_1 ← DNA‑directed RNA polymerase subunit alpha
RA USA300TCH1516_ALE 2,372,751 A→T 68.8% L342* (TTA→TAA)  pbuG ← Guanine/hypoxanthine permease PbuG
RA USA300TCH1516_ALE 2,373,531 G→T 8.2% A82E (GCG→GAG)  pbuG ← Guanine/hypoxanthine permease PbuG
RA USA300TCH1516_ALE 2,373,714 C→T 5.7% G21D (GGT→GAT)  pbuG ← Guanine/hypoxanthine permease PbuG
RA USA300TCH1516_ALE 2,462,758 G→A 61.7% A70T (GCA→ACA)  USA300HOU_RS12680 → Putative 3‑methyladenine DNA glycosylase
RA USA300TCH1516_ALE 2,532,298 A→T 100% T15T (ACA→ACT femA_3 → Aminoacyltransferase FemA
RA USA300TCH1516_ALE 2,774,816 C→T 6.8% S790S (TCG→TCA clfB ← Clumping factor B
RA USA300TCH1516_ALE 2,774,855 A→G 5.4% D777D (GAT→GAC clfB ← Clumping factor B
RA USA300TCH1516_ALE 2,774,930 C→T 6.4% S752S (TCG→TCA clfB ← Clumping factor B
RA USA300TCH1516_ALE 2,774,981 A→G 6.5% D735D (GAT→GAC clfB ← Clumping factor B
RA USA300TCH1516_ALE 2,775,041 A→G 5.6% D715D (GAT→GAC clfB ← Clumping factor B
RA USA300TCH1516_ALE 2,775,089 A→G 7.7% D699D (GAT→GAC clfB ← Clumping factor B
RA USA300TCH1516_ALE 2,775,287 A→G 5.7% D633D (GAT→GAC clfB ← Clumping factor B

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 52495 =282 (0.770)342 (0.950)
+T
9/258 NT 53.3% intergenic (+105/‑568) USA300HOU_RS00215/USA300HOU_RS00220 hypothetical protein/hypothetical protein
?USA300TCH1516_ALE = 52988 321 (0.880)intergenic (+598/‑75) USA300HOU_RS00215/USA300HOU_RS00220 hypothetical protein/hypothetical protein
* ? USA300TCH1516_ALE 512620 =394 (1.080)278 (1.100)
+40 bp
108/180 NT 50.6% intergenic (+688/‑6075) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE 1998278 = NA (NA)intergenic (‑6/+3329) USA300TCH1516_01882/USA300TCH1516_01883 tRNA‑Val/tRNA‑Ala
* ? USA300TCH1516_ALE 895785 =318 (0.870)29 (0.090) 23/226 NT 9.5% coding (806/822 nt) metQ_2 Methionine‑binding lipoprotein MetQ
?USA300TCH1516_ALE = 909740 276 (0.870)intergenic (‑663/‑371) USA300HOU_RS04500/csbD_1 hypothetical protein/Stress response protein CsbD
* ? USA300TCH1516_ALE 2085296 =302 (0.830)25 (0.080) 20/232 NT 8.2% coding (173/201 nt) hlb_1 Phospholipase C
?USA300TCH1516_ALE = 2128343 294 (0.900)coding (1/825 nt) hlb_2 Phospholipase C