breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 132,185 G→A 5.6% D560N (GAC→AAC)  fusA → elongation factor G
RA 316,262 C→A 8.0% Q28K (CAA→AAA)  opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA
RA 376,966 A→C 9.2% K781T (AAA→ACA)  srfAA → surfactin non‑ribosomal peptide synthetase SrfAA
RA 438,792 C→A 8.7% intergenic (‑58/‑48) gabR ← / → gabT transcriptional regulator GabR/4‑aminobutyrate‑‑2‑oxoglutarate transaminase
RA 438,810 G→T 6.0% intergenic (‑76/‑30) gabR ← / → gabT transcriptional regulator GabR/4‑aminobutyrate‑‑2‑oxoglutarate transaminase
RA 452,125 T→A 46.3% K146I (AAA→ATA)  EO946_RS02320 ← dienelactone hydrolase family protein
RA 549,057 C→A 5.4% F23L (TTC→TTA EO946_RS02865 → PLP‑dependent aminotransferase family protein
RA 607,260 G→C 6.2% A35P (GCC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,359 A→C 15.6% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,514 T→G 7.1% A119A (GCT→GCG EO946_RS03185 → collagen‑like protein
RA 607,682 G→C 5.3% A175A (GCG→GCC EO946_RS03185 → collagen‑like protein
RA 607,700 A→C 19.4% E181D (GAA→GAC EO946_RS03185 → collagen‑like protein
RA 607,838 A→G 11.9% T227T (ACA→ACG EO946_RS03185 → collagen‑like protein
RA 607,847 G→A 6.9% T230T (ACG→ACA EO946_RS03185 → collagen‑like protein
RA 607,853 C→T 7.5% A232A (GCC→GCT EO946_RS03185 → collagen‑like protein
RA 607,856 A→T 6.1% T233T (ACA→ACT EO946_RS03185 → collagen‑like protein
RA 755,531 A→C 9.9% Q22H (CAA→CAC EO946_RS03880 → rhamnogalacturonan acetylesterase
RA 822,230 T→G 6.2% A252A (GCT→GCG EO946_RS04190 → sodium:alanine symporter family protein
RA 1,046,159 A→G 6.5% H73H (CAT→CAC EO946_RS05410 ← HTH‑type transcriptional regulator Hpr
RA 1,112,969 C→T 100% L1022F (CTT→TTT)  addB → helicase‑exonuclease AddAB subunit AddB
RA 1,151,967 T→G 12.6% K79T (AAA→ACA)  EO946_RS05920 ← GNAT family N‑acetyltransferase
RA 1,450,016 G→C 5.1% P59P (CCG→CCC ccpC → transcriptional regulator CcpC
RA 1,463,824 G→T 10.1% D44Y (GAT→TAT)  EO946_RS07665 → ABC transporter ATP‑binding protein/permease
RA 1,488,158 G→T 9.6% D567Y (GAT→TAT)  ade → adenine deaminase
RA 1,488,371 G→T 16.2% S509* (TCA→TAA)  rnjA ← ribonuclease J1
RA 1,512,543 G→T 5.9% intergenic (‑71/‑42) EO946_RS07905 ← / → typA YlaF family protein/translational GTPase TypA
RA 1,514,881 G→T 7.4% Q62K (CAA→AAA)  EO946_RS07920 ← YlaI family protein
RA 1,570,501 A→T 5.6% A62A (GCA→GCT spoIIGA → sigma‑E processing peptidase SpoIIGA
RA 1,570,504 T→G 5.2% G63G (GGT→GGG spoIIGA → sigma‑E processing peptidase SpoIIGA
RA 1,570,510 G→A 5.2% L65L (CTG→CTA spoIIGA → sigma‑E processing peptidase SpoIIGA
RA 2,214,518 T→G 5.6% K60T (AAA→ACA)  nth ← endonuclease III
RA 2,300,863 T→G 7.0% intergenic (‑194/+202) ribD ← / ← EO946_RS11710 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase RibD/hypothetical protein
RA 2,350,847 G→T 7.9% pseudogene (934/1182 nt) EO946_RS12020 ← IS3 family transposase
RA 2,350,848 A→T 14.2% pseudogene (933/1182 nt) EO946_RS12020 ← IS3 family transposase
RA 2,425,640 G→T 6.3% intergenic (+4/‑17) EO946_RS12410 → / → EO946_RS12415 DEAD/DEAH box helicase/YqhG family protein
RA 2,502,242 T→G 18.3% T274P (ACG→CCG)  hrcA ← heat‑inducible transcriptional repressor HrcA
RA 2,680,400 A→T 10.3% V417D (GTT→GAT)  EO946_RS13875 ← LysM peptidoglycan‑binding domain‑containing protein
RA 2,775,852 A→G 5.4% G145G (GGT→GGC EO946_RS14350 ← TVP38/TMEM64 family protein
RA 2,775,864 A→G 5.6% S141S (AGT→AGC EO946_RS14350 ← TVP38/TMEM64 family protein
RA 2,775,870 A→G 6.3% F139F (TTT→TTC EO946_RS14350 ← TVP38/TMEM64 family protein
RA 2,775,897 T→C 6.1% L130L (TTA→TTG EO946_RS14350 ← TVP38/TMEM64 family protein
RA 2,775,912 A→G 5.9% F125F (TTT→TTC EO946_RS14350 ← TVP38/TMEM64 family protein
RA 2,865,472 C→A 8.3% D116Y (GAT→TAT)  pulA ← type I pullulanase
RA 2,982,236 T→C 27.3% noncoding (284/1550 nt) EO946_RS15435 ← 16S ribosomal RNA
RA 2,991,262 G→T 24.4% intergenic (+63/‑144) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA
RA 3,247,065 C→T 100% intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
RA 3,268,712 G→T 9.5% intergenic (+60/+150) catR → / ← EO946_RS16920 catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase
RA 3,268,725 C→A 10.9% intergenic (+73/+137) catR → / ← EO946_RS16920 catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase
RA 3,280,312 C→A 5.3% L136F (TTG→TTT EO946_RS16965 ← lantibiotic dehydratase
RA 3,280,341 C→A 8.8% E127* (GAG→TAG)  EO946_RS16965 ← lantibiotic dehydratase
RA 3,285,567 C→T 36.6% L278L (CTG→CTA EO946_RS16995 ← IS3 family transposase
RA 3,285,575 T→G 37.8% N276H (AAT→CAT)  EO946_RS16995 ← IS3 family transposase
RA 3,285,580 A→G 39.3% I274T (ATA→ACA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,647 C→A 39.2% V252L (GTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,713 G→A 27.4% L230L (CTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,328,921 G→A 5.1% S258S (TCG→TCA lutP → L‑lactate permease LutP
RA 3,447,038 T→G 10.4% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU
RA 3,496,441 C→G 5.8% L329L (CTG→CTC EO946_RS17995 ← glucosaminidase domain‑containing protein
RA 3,496,459 C→A 6.2% G323G (GGG→GGT EO946_RS17995 ← glucosaminidase domain‑containing protein
RA 3,637,344 T→A 7.6% Y44N (TAC→AAC)  EO946_RS18740 → DUF2529 domain‑containing protein
RA 3,746,962 C→A 6.7% D36Y (GAT→TAT)  EO946_RS19275 ← galactokinase
RA 3,790,764 T→G 19.9% N203H (AAC→CAC)  EO946_RS19525 ← BglG family transcription antiterminator
RA 3,993,028 G→A 6.4% S211L (TCG→TTG)  walK ← cell wall metabolism sensor histidine kinase WalK
RA 3,994,110 C→G 6.0% V89L (GTC→CTC)  walR ← cell wall metabolism DNA‑binding response regulator WalR
RA 3,996,851 A→T 9.0% intergenic (‑157/+49) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein
RA 3,996,891 G→T 8.8% intergenic (‑197/+9) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 91969NA (NA)18 (0.070) 8/250 NT NA intergenic (+131/‑39) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
?NZ_CP034943 91973 = NA (NA)intergenic (+135/‑35) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA