breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 5,439 A→C 13.6% H69P (CAC→CCC)  Y75_RS00030 → DUF2502 domain‑containing protein
RA 12,484 C→A 21.1% Q108K (CAG→AAG)  dnaK → molecular chaperone DnaK
RA 53,165 G→A 27.5% V84V (GTC→GTT pdxA ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase PdxA
RA 82,766 A→G 9.3% L255P (CTC→CCC)  leuA ← 2‑isopropylmalate synthase
RA 210,994 T→C 31.0% V439A (GTC→GCC)  ldcC → lysine decarboxylase LdcC
RA 226,042 T→C 24.7% noncoding (293/2930 nt) Y75_RS01035 → 23S ribosomal RNA
RA 228,688 Δ1 bp 100% intergenic (+9/‑70) Y75_RS01035 → / → rrf 23S ribosomal RNA/5S ribosomal RNA
RA 249,538 T→G 20.9% H187P (CAC→CCC)  flhA ← flagellar type III secretion system protein FlhA
RA 249,587 T→G 13.2% T171P (ACC→CCC)  flhA ← flagellar type III secretion system protein FlhA
RA 282,321 T→C 10.9% S274P (TCC→CCC)  yagE → 2‑keto‑3‑deoxygluconate aldolase
RA 284,748 A→G 10.0% I44V (ATT→GTT)  Y75_RS01395 → MFS transporter
RA 346,895 G→C 10.6% A258G (GCG→GGG)  prpR ← propionate catabolism operon regulatory protein PrpR
RA 359,586 T→G 16.4% V133G (GTG→GGG)  cynX → cyanate transporter CynX
RA 380,685 G→A 9.8% R52R (CGG→CGA Y75_RS01860 → IS3‑like element IS2 family transposase
RA 380,686 C→T 10.2% Q53* (CAA→TAA)  Y75_RS01860 → IS3‑like element IS2 family transposase
RA 386,965 A→G 26.2% E257E (GAA→GAG tauC → taurine ABC transporter permease TauC
RA 387,473 A→G 15.2% E152G (GAG→GGG)  tauD → taurine dioxygenase
RA 411,312 T→C 17.1% M132V (ATG→GTG)  araJ ← MFS transporter AraJ
RA 421,455 A→T 10.6% I416F (ATC→TTC)  proY → proline‑specific permease ProY
RA 432,873 T→G 27.3% G65G (GGT→GGG ribD → bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase RibD
RA 442,627 T→C 9.5% E80G (GAG→GGG)  yajL ← protein deglycase YajL
RA 449,374 T→C 27.4% E164E (GAA→GAG cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 471,229 A→G 11.2% E457G (GAG→GGG)  Y75_RS02310 → SmdB family multidrug efflux ABC transporter permease/ATP‑binding protein
RA 479,721 T→C 25.1% K71R (AAG→AGG)  tomB ← Hha toxicity modulator TomB
RA 502,111 T→A 12.4% I118F (ATC→TTC)  Y75_RS02460 ← Kef family K(+) transporter
RA 525,193 A→G 23.8% A903A (GCA→GCG Y75_RS02555 → rhs element protein RhsD
RA 539,770 C→T 8.0% intergenic (+38/‑19) allB → / → Y75_RS02625 allantoinase AllB/uracil/xanthine transporter
RA 539,835 A→T 8.1% Q16L (CAG→CTG)  Y75_RS02625 → uracil/xanthine transporter
RA 547,694 A→G 100% E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,831:1 +G 100% coding (251/261 nt) Y75_RS02655 → hypothetical protein
RA 550,454 A→G 11.8% S265G (AGT→GGT)  Y75_RS02670 → carbamate kinase
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
RA 582,170 A→G 22.8% pseudogene (73/261 nt) Y75_RS02905 → tail fiber assembly protein
RA 591,407 A→G 26.8% I332T (ATC→ACC)  nrfB ← phage adsorption protein NrfB
RA 629,331 T→G 9.8% V72G (GTG→GGG)  cstA → carbon starvation protein CstA
RA 629,410 A→G 7.0% G98G (GGA→GGG cstA → carbon starvation protein CstA
RA 632,320 T→C 25.5% T127T (ACA→ACG hcxA ← hydroxycarboxylate dehydrogenase HcxA
RA 642,258 T→C 18.4% G316G (GGT→GGC Y75_RS03155 → glutathione‑dependent formaldehyde dehydrogenase
MC JC 654,214 Δ1,199 bp 100% Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
RA 801,248 A→G 29.6% H23R (CAT→CGT)  Y75_RS04015 → 4‑oxalomesaconate tautomerase
RA 837,020 T→C 12.8% L83P (CTC→CCC)  Y75_RS04175 → hydroxycarboxylate dehydrogenase HcXB
RA 864,182 A→G 10.3% D40G (GAT→GGT)  fsa → fructose‑6‑phosphate aldolase
RA 916,131 G→A 21.9% A113A (GCC→GCT aqpZ ← aquaporin Z
RA 926,681 A→T 66.5% L62Q (CTG→CAG)  infA ← translation initiation factor IF‑1
RA 954,782 T→A 12.5% Y36F (TAT→TTT)  focA ← formate transporter FocA
RA 954,817 G→A 10.3% V24V (GTC→GTT focA ← formate transporter FocA
RA 1,037,257 T→C 11.1% C29C (TGT→TGC Y75_RS05085 → hydrogenase expression/formation protein
RA 1,042,668 A→C 5.7% S655R (AGT→AGG etk ← tyrosine‑protein kinase
RA 1,065,700 C→T 100% R415C (CGT→TGT)  Y75_RS05220 → YccE family protein
RA 1,093,686 T→C 100% V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
RA 1,116,546 T→A 9.7% V174V (GTA→GTT mdtG ← multidrug efflux MFS transporter MdtG
RA 1,146,870 T→G 12.9% G118G (GGT→GGG rluC → 23S rRNA pseudouridine(955/2504/2580) synthase RluC
RA 1,158,744 A→G 35.4% T131A (ACC→GCC)  Y75_RS05755 → metal‑dependent hydrolase
RA 1,163,039 T→G 10.2% L30F (TTA→TTC fhuE ← ferric‑rhodotorulic acid/ferric‑coprogen receptor FhuE
RA 1,239,439 A→C 9.9% T98P (ACC→CCC)  Y75_RS06220 → D‑amino acid dehydrogenase
RA 1,256,357 T→C 12.1% G391G (GGA→GGG Y75_RS06285 ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA 1,303,712 A→G 100% S273G (AGC→GGC)  oppA → oligopeptide ABC transporter substrate‑binding protein OppA
RA 1,305,677 A→G 8.8% I44V (ATA→GTA)  oppC → oligopeptide ABC transporter permease OppC
RA 1,324,092 C→T 11.7% S190N (AGC→AAC)  trpE ← anthranilate synthase component I
JC 1,328,313 Δ36 bp 12.0% coding (1854‑1889/1896 nt) Y75_RS06660 → DUF2207 domain‑containing protein
RA 1,335,007 C→T 24.9% A749V (GCA→GTA)  topA → type I DNA topoisomerase
RA 1,349,026 C→T 29.7% R534H (CGT→CAT)  rnb ← exoribonuclease II
RA 1,350,440 C→T 31.8% V63M (GTG→ATG)  rnb ← exoribonuclease II
RA 1,378,785 A→C 12.9% S80S (TCA→TCC Y75_RS06935 → Gfo/Idh/MocA family oxidoreductase
RA 1,379,403 T→A 8.9% D286E (GAT→GAA Y75_RS06935 → Gfo/Idh/MocA family oxidoreductase
RA 1,392,432 G→A 23.8% intergenic (‑120/+7) mpaA ← / ← Y75_RS06995 murein tripeptide amidase MpaA/NAD‑dependent dehydratase
RA 1,406,309 A→G 21.7% intergenic (‑30/‑146) Y75_RS07065 ← / → Y75_RS07070 p‑aminobenzoyl‑glutamate hydrolase subunit AbgA/LysR family transcriptional regulator
MC JC 1,413,639 Δ23,060 bp 100% [ttcA]Y75_RS24865 33 genes
RA 1,440,143 A→G 26.6% F786L (TTC→CTC)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,447,718 T→G 10.5% V42G (GTT→GGT)  Y75_RS07310 → YdbL family protein
RA 1,513,839 A→G 25.5% T158A (ACG→GCG)  Y75_RS07610 → ABC transporter substrate‑binding protein
RA 1,515,716 A→T 9.4% T58S (ACT→TCT)  Y75_RS07620 → ABC transporter permease
RA 1,516,725 A→G 21.9% T84A (ACG→GCG)  Y75_RS07625 → ABC transporter permease
RA 1,521,798 G→A 11.0% intergenic (+20/‑178) curA → / → Y75_RS07665 NADPH‑dependent curcumin/dihydrocurcumin reductase/DNA‑binding transcriptional regulator McbR
RA 1,530,091 A→G 27.0% Y80C (TAC→TGC)  Y75_RS07695 → RHS repeat protein
RA 1,535,967 A→T 10.6% E77V (GAG→GTG)  nhoA → N‑hydroxyarylamine O‑acetyltransferase
RA 1,562,499 T→A 15.9% I49F (ATC→TTC)  Y75_RS07840 ← ABC transporter permease
RA 1,569,576 T→C 31.4% Y321C (TAC→TGC)  Y75_RS07865 ← glycoside hydrolase family 10 protein
RA 1,578,891 A→G 20.8% V148A (GTG→GCG)  Y75_RS07890 ← TonB‑dependent receptor plug domain‑containing protein
RA 1,600,253 C→T 34.9% intergenic (‑453/+78) Y75_RS07965 ← / ← lsrK autotransporter barrel domain‑containing lipoprotein/autoinducer‑2 kinase
RA 1,613,633 C→A 8.2% intergenic (‑65/+47) Y75_RS08030 ← / ← Y75_RS08035 GGDEF domain‑containing protein/DUF4186 domain‑containing protein
RA 1,629,848 A→G 26.8% A206A (GCA→GCG ydfG → bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG
RA 1,648,781 T→C 24.4% intergenic (+330/+107) Y75_RS08260 → / ← Y75_RS08265 protein FlxA/hypothetical protein
RA 1,667,148 T→C 25.1% S52S (AGT→AGC clcB → voltage‑gated ClC‑type chloride channel ClcB
RA 1,668,443 A→G 27.0% L164P (CTG→CCG)  bioD ← ATP‑dependent dethiobiotin synthetase BioD
RA 1,718,276 A→G 26.5% F221L (TTT→CTT)  tyrS ← tyrosine‑‑tRNA ligase
RA 1,739,298 A→G 100% intergenic (‑39/‑260) ynhF ← / → purR stress response protein YnhF/HTH‑type transcriptional repressor PurR
RA 1,756,553 G→A 20.9% intergenic (‑362/+93) Y75_RS08815 ← / ← fumD ferredoxin‑like protein/fumarate hydratase FumD
RA 1,757,345 G→A 32.1% intergenic (‑490/‑67) fumD ← / → Y75_RS08825 fumarate hydratase FumD/pyruvate kinase PykF
RA 1,775,399 T→C 23.9% Y392H (TAT→CAT)  Y75_RS08905 → MFS transporter
RA 1,802,255 T→C 28.7% Q33R (CAG→CGG)  infC ← translation initiation factor IF‑3
RA 1,813,070 T→C 25.3% G141G (GGT→GGC Y75_RS09105 → cystine/sulfocysteine:cation symporter
RA 1,823,202 A→C 68.0% A44A (GCT→GCG chbB ← PTS N,N'‑diacetylchitobiose transporter subunit IIB
RA 1,824,414 A→G 20.9% V81V (GTA→GTG nadE → ammonia‑dependent NAD(+) synthetase
RA 1,825,992 C→A 14.7% T255K (ACA→AAA)  cho → excinuclease Cho
RA 1,845,615 T→C 29.5% H324R (CAC→CGC)  Y75_RS09275 ← DUF1266 domain‑containing protein
RA 1,858,002 T→C 18.1% D214G (GAC→GGC)  Y75_RS09335 ← carbohydrate kinase family protein
RA 1,859,322 A→G 25.3% G54G (GGT→GGC Y75_RS09340 ← ketose‑bisphosphate aldolase
RA 1,889,234 A→C 7.8% Y154D (TAT→GAT)  rnd ← ribonuclease D
RA 1,905,809 T→C 25.5% R67R (CGT→CGC Y75_RS09580 → PTS mannose transporter subunit IID
RA 1,908,490 A→C 13.0% G95G (GGT→GGG rlmA ← 23S rRNA (guanine(745)‑N(1))‑methyltransferase
RA 1,920,690 T→G 15.0% G489G (GGT→GGG yebT → lipid‑binding membrane homeostasis protein YebT
RA 1,928,151 G→A 24.6% L106L (TTG→TTA exoX → exodeoxyribonuclease X
RA 1,930,726 C→T 5.1% intergenic (‑173/+36) ptrB ← / ← Y75_RS09735 oligopeptidase B/tellurite resistance TerB family protein
RA 1,932,029 T→C 27.1% T26A (ACC→GCC)  yebF ← protein YebF
RA 1,951,939 T→C 32.5% T100A (ACT→GCT)  aspS ← aspartate‑‑tRNA ligase
RA 1,986,282 T→G 15.0% T309P (ACC→CCC)  Y75_RS10005 ← arabinose ABC transporter ATP binding protein AraG
RA 1,991,081 T→C 18.5% F77L (TTT→CTT)  ftnA → non‑heme ferritin
RA 2,022,089 T→C 26.6% V112A (GTA→GCA)  fliL → flagellar basal body‑associated protein FliL
RA 2,036,246 A→G 20.8% pseudogene (59/1193 nt) Y75_RS24930 → porin
RA 2,042,264 T→C 17.2% L217P (CTC→CCC)  msrP → protein‑methionine‑sulfoxide reductase catalytic subunit MsrP
RA 2,042,280 A→G 20.8% V222V (GTA→GTG msrP → protein‑methionine‑sulfoxide reductase catalytic subunit MsrP
RA 2,054,843 T→C 26.1% pseudogene (623/1053 nt) Y75_RS10400 ← glycosyltransferase family 9 protein
RA 2,074,477 A→G 20.8% T268A (ACT→GCT)  ag43 → autotransporter adhesin Ag43
RA 2,081,558 C→T 32.8% intergenic (‑60/+112) Y75_RS10545 ← / ← Y75_RS10550 DUF496 family protein/FUSC family protein
RA 2,128,340 T→C 22.9% D7G (GAC→GGC)  Y75_RS10765 ← GDP‑mannose mannosyl hydrolase
RA 2,135,997 G→A 23.1% P598L (CCG→CTG)  wzc ← tyrosine‑protein kinase Wzc
RA 2,147,193 T→G 8.4% G597G (GGT→GGG Y75_RS10845 → diguanylate cyclase
RA 2,152,044 A→C 26.5% V360G (GTG→GGG)  yegI ← protein kinase YegI
RA 2,153,826 G→T 33.9% intergenic (+43/+22) Y75_RS10870 → / ← pphC YegJ family protein/protein‑serine/threonine phosphatase PphC
RA 2,153,827 T→C 38.1% intergenic (+44/+21) Y75_RS10870 → / ← pphC YegJ family protein/protein‑serine/threonine phosphatase PphC
MC JC 2,176,983 Δ1,199 bp 100% Y75_RS10980[gatA] Y75_RS10980, [gatA]
RA 2,199,942 T→C 32.7% pseudogene (135/3796 nt) Y75_RS24960 → WGR and DUF4132 domain‑containing protein
RA 2,202,338 A→G 7.6% pseudogene (2531/3796 nt) Y75_RS24960 → WGR and DUF4132 domain‑containing protein
RA 2,212,091 A→G 17.6% D264G (GAC→GGC)  Y75_RS11125 → VWA domain‑containing protein
RA 2,213,155 T→C 9.8% pseudogene (722/2001 nt) Y75_RS11130 → dipeptidase
RA 2,237,845 G→A 30.6% G160D (GGT→GAT)  Y75_RS11255 → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
RA 2,248,798 G→A 7.4% R436C (CGT→TGT)  cirA ← catecholate siderophore receptor CirA
RA 2,258,308 A→G 23.3% I71I (ATT→ATC rihB ← ribosylpyrimidine nucleosidase
RA 2,315,451 A→C 15.4% G211G (GGT→GGG ompC ← porin OmpC
MC JC 2,320,795 Δ1,336 bp 100% Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,328,463 G→A 25.8% G116D (GGT→GAT)  atoD → acetate CoA‑transferase subunit alpha
RA 2,355,566 G→A 23.0% A39V (GCC→GTC)  glpQ ← glycerophosphodiester phosphodiesterase
RA 2,372,009 A→C 19.4% H90P (CAC→CCC)  arnC → undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase
RA 2,372,048 T→C 20.8% L103P (CTC→CCC)  arnC → undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase
RA 2,471,949 T→C 19.8% intergenic (+120/‑42) Y75_RS12330 → / → intS tRNA‑Arg/prophage integrase IntS
RA 2,472,663 A→G 25.0% T225A (ACC→GCC)  intS → prophage integrase IntS
RA 2,509,498 A→G 11.6% I145T (ATC→ACC)  ptsP ← phosphoenolpyruvate‑‑protein phosphotransferase
RA 2,512,955 G→A 23.9% P129L (CCG→CTG)  Y75_RS12530 ← PTS fructose transporter subunit IIC
RA 2,527,901 T→A 5.0% D8V (GAT→GTT)  xapR ← DNA‑binding transcriptional regulator XapR
RA 2,579,066 G→A 24.2% A259T (GCG→ACG)  tkt → transketolase
RA 2,606,887 A→G 21.4% M439V (ATG→GTG)  hyfF → hydrogenase 4 subunit F
RA 2,645,910 T→C 24.7% A24A (GCA→GCG pbpC ← peptidoglycan glycosyltransferase PbpC
RA 2,647,907 T→C 28.5% S1013G (AGT→GGT)  yfhM ← alpha2‑macroglobulin
RA 2,660,496 A→T 73.9% C98S (TGC→AGC)  iscR ← Fe‑S cluster assembly transcriptional regulator IscR
RA 2,664,592 A→G 8.2% T168A (ACG→GCG)  csiE → stationary phase inducible protein CsiE
RA 2,673,614 T→C 21.1% G279G (GGA→GGG Y75_RS13315 ← alcohol dehydrogenase catalytic domain‑containing protein
RA 2,676,704 A→C 12.2% V105G (GTG→GGG)  Y75_RS13325 ← sugar ABC transporter ATP‑binding protein
RA 2,746,219 A→G 21.2% V78A (GTC→GCC)  ffh ← signal recognition particle protein
RA 2,763,433 T→C 22.7% N126S (AAC→AGC)  Y75_RS13760 ← anti‑bacteriophage protein AbpA
RA 2,789,650 T→C 18.7% L338P (CTC→CCC)  Y75_RS13885 → L‑2‑hydroxyglutarate oxidase
RA 2,792,331 T→G 9.5% V314G (GTG→GGG)  Y75_RS13895 → 4‑aminobutyrate‑‑2‑oxoglutarate transaminase
RA 2,793,721 T→C 30.3% G271G (GGT→GGC gabP → GABA permease
RA 2,813,572 T→C 25.2% E508E (GAA→GAG gshA ← glutamate‑‑cysteine ligase
RA 2,813,659 C→T 24.8% P479P (CCG→CCA gshA ← glutamate‑‑cysteine ligase
RA 2,837,365 T→C 27.6% T187A (ACC→GCC)  Y75_RS14155 ← DNA‑binding transcriptional regulator AscG
RA 2,855,834 T→A 22.3% L29Q (CTG→CAG)  mutS → DNA mismatch repair protein MutS
RA 2,866,109 C→A 100% pseudogene (99/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 2,877,551 A→G 25.8% L293P (CTT→CCT)  cas1e ← type I‑E CRISPR‑associated endonuclease Cas1
RA 2,906,975 C→G 100% D450H (GAC→CAC)  pyrG ← CTP synthase (glutamine hydrolyzing)
RA 2,908,235 C→A 13.8% E30* (GAA→TAA)  pyrG ← CTP synthase (glutamine hydrolyzing)
JC 2,910,136 Δ12 bp 34.3% coding (2161‑2172/2235 nt) Y75_RS14515 ← GTP pyrophosphokinase
RA 2,910,944 A→T 70.5% L455Q (CTG→CAG)  Y75_RS14515 ← GTP pyrophosphokinase
RA 2,962,177 C→T 22.2% G66S (GGT→AGT)  Y75_RS14725 ← prepilin peptidase‑dependent protein
RA 2,963,338 T→C 10.0% K158K (AAA→AAG thyA ← thymidylate synthase
RA 2,973,749 T→G 10.7% T308P (ACT→CCT)  aas ← bifunctional acyl‑ACP‑‑phospholipid O‑acyltransferase/long‑chain‑fatty‑acid‑‑ACP ligase
RA 3,013,385 A→G 32.0% V110A (GTG→GCG)  yqeC ← putative selenium‑dependent hydroxylase accessory protein YqeC
RA 3,131,026 G→C 9.8% G13G (GGC→GGG Y75_RS15535 ← lipopolysaccharide biosynthesis protein
RA 3,134,422 G→T 8.3% G202G (GGC→GGA pitB ← inorganic phosphate transporter PitB
RA 3,184,681 A→G 6.0% intergenic (+60/‑117) Y75_RS15835 → / → Y75_RS15845 fimbrial‑like protein/IS3‑like element IS2 family transposase
RA 3,205,094 G→A 19.1% intergenic (‑182/‑25) ttdR ← / → ttdA DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
RA 3,205,127 C→G 10.6% S3R (AGC→AGG ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,133 T→A 19.0% S5R (AGT→AGA ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,140 C→A 14.4% Q8K (CAA→AAA)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,143 C→A 13.7% Q9K (CAG→AAG)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,162 C→A 14.2% T15K (ACA→AAA)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,206,583 G→A 21.1% R186H (CGC→CAC)  ttdB → L(+)‑tartrate dehydratase subunit beta
MC JC 3,261,134 Δ1,199 bp 100% Y75_RS16205[tdcD] Y75_RS16205, [tdcD]
RA 3,305,174 T→G 6.0% T167P (ACC→CCC)  mtr ← tryptophan permease
RA 3,306,710 T→C 25.7% T336A (ACC→GCC)  Y75_RS16445 ← ATP‑dependent RNA helicase DeaD
RA 3,329,674 T→G 12.5% V286G (GTA→GGA)  dacB → serine‑type D‑Ala‑D‑Ala carboxypeptidase
RA 3,331,036 T→A 10.5% T192S (ACC→TCC)  cgtA ← Obg family GTPase CgtA
RA 3,336,758 C→T 36.8% intergenic (‑100/+60) ibaG ← / ← mlaB BolA family iron metabolism protein IbaG/lipid asymmetry maintenance protein MlaB
RA 3,365,322 G→A 21.3% pseudogene (283/369 nt) Y75_RS16720 → hypothetical protein
RA 3,372,994 T→C 25.1% K146K (AAA→AAG nanA ← N‑acetylneuraminate lyase
RA 3,441,668 T→G 13.0% C363G (TGC→GGC)  thiC → phosphomethylpyrimidine synthase ThiC
RA 3,450,775 T→C 24.8% Q186R (CAG→CGG)  rpoC ← DNA‑directed RNA polymerase subunit beta'
RA 3,485,352 A→C 26.4% A590A (GCA→GCC ppc → phosphoenolpyruvate carboxylase
RA 3,510,452 T→G 14.0% G194G (GGT→GGG priA → primosomal protein N'
RA 3,521,463 A→G 10.0% E121G (GAG→GGG)  glpX → class II fructose‑bisphosphatase
RA 3,602,373 T→C 23.0% A388A (GCA→GCG trkH ← Trk system potassium transporter TrkH
RA 3,606,626 T→C 26.6% L306P (CTC→CCC)  fadB → fatty acid oxidation complex subunit alpha FadB
RA 3,616,272 G→T 9.9% Q62K (CAA→AAA)  ubiB ← ubiquinone biosynthesis regulatory protein kinase UbiB
RA 3,620,608 A→C 11.7% T33P (ACC→CCC)  Y75_RS17970 → dienelactone hydrolase family protein
RA 3,645,110 A→T 100% L140Q (CTG→CAG)  cyaA ← class I adenylate cyclase
RA 3,645,220 G→A 30.4% S103S (AGC→AGT cyaA ← class I adenylate cyclase
RA 3,648,106 T→C 22.0% S164S (AGT→AGC hemX → uroporphyrinogen‑III C‑methyltransferase
RA 3,648,353 C→A 9.4% Q247K (CAA→AAA)  hemX → uroporphyrinogen‑III C‑methyltransferase
RA 3,656,455 C→T 100% V91I (GTT→ATT)  rffM ← lipopolysaccharide N‑acetylmannosaminouronosyltransferase
RA 3,690,546 T→C 28.6% noncoding (2441/2925 nt) Y75_RS18325 ← 23S ribosomal RNA
RA 3,708,445 A→G 30.6% K456K (AAA→AAG viaA → ATPase RavA stimulator ViaA
RA 3,715,545 T→G 63.5% G18G (GGT→GGG atpE → F0F1 ATP synthase subunit C
RA 3,741,125 A→G 23.0% M352V (ATG→GTG)  Y75_RS18545 → adenine permease AdeP
MC JC 3,742,936 Δ1,336 bp 100% Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
RA 3,745,991 T→C 33.8% I138V (ATC→GTC)  mdtL ← multidrug efflux MFS transporter MdtL
MC JC 3,746,911 Δ1,199 bp 100% Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,750,591 Δ1,199 bp 100% Y75_RS18600 Y75_RS18600
RA 3,777,745 A→T 31.0% I312F (ATT→TTT)  Y75_RS18735 → PTS transporter subunit EIIC
RA 3,777,754 A→C 32.5% T315P (ACC→CCC)  Y75_RS18735 → PTS transporter subunit EIIC
RA 3,790,458 G→A 19.8% W60* (TGG→TGA uhpB → signal transduction histidine‑protein kinase/phosphatase UhpB
RA 3,797,639 C→G 6.4% G338G (GGC→GGG Y75_RS18845 → adenine permease AdeQ
RA 3,810,061 C→T 13.2% intergenic (‑103/+18) Y75_RS18905 ← / ← xanP AsmA family protein/xanthine/proton symporter XanP
RA 3,814,831 A→C 7.8% G125G (GGT→GGG recG ← ATP‑dependent DNA helicase RecG
RA 3,820,133 G→A 31.2% A297T (GCA→ACA)  ligB → NAD‑dependent DNA ligase LigB
RA 3,831,076 A→G 34.3% V267A (GTA→GCA)  waaA ← 3‑deoxy‑D‑manno‑octulosonic acid transferase
RA 3,857,502 T→G 18.9% V250G (GTG→GGG)  gpsA → NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
RA 3,894,855 T→G 13.0% T253P (ACA→CCA)  yiaN ← 2,3‑diketo‑L‑gulonate transporter large permease YiaN
RA 3,908,726 G→T 14.9% Q187K (CAA→AAA)  Y75_RS19370 ← D‑xylose ABC transporter substrate‑binding protein
RA 3,935,497 A→G 11.1% G291G (GGA→GGG dppB → dipeptide ABC transporter permease DppB
RA 3,953,876 A→G 10.0% S879G (AGC→GGC)  bcsC → cellulose biosynthesis protein BcsC
RA 3,955,895 T→C 23.1% F367L (TTC→CTC)  hmsP → biofilm formation regulator HmsP
RA 3,994,332 A→C 13.3% R233R (CGT→CGG Y75_RS19765 ← 23S rRNA (adenine(2030)‑N(6))‑methyltransferase
RA 4,002,969 A→G 29.1% intergenic (‑196/‑36) pitA ← / → Y75_RS19800 inorganic phosphate transporter PitA/NAD(P)/FAD‑dependent oxidoreductase
RA 4,017,560 G→A 29.9% P1222S (CCG→TCG)  Y75_RS19860 ← rhs element protein RhsB
RA 4,024,550 T→G 11.4% T209P (ACC→CCC)  nikB ← nickel ABC transporter permease subunit NikB
RA 4,041,774 A→G 26.4% T340A (ACC→GCC)  Y75_RS19970 → branched chain amino acid ABC transporter substrate‑binding protein LivJ
RA 4,069,227 A→G 23.0% E44E (GAA→GAG glgX → glycogen debranching protein GlgX
RA 4,071,173 T→G 14.9% R29R (CGT→CGG glgC → glucose‑1‑phosphate adenylyltransferase
RA 4,071,644 C→A 14.6% N186K (AAC→AAA glgC → glucose‑1‑phosphate adenylyltransferase
RA 4,095,881 A→G 22.0% T104A (ACC→GCC)  bioH → pimeloyl‑ACP methyl ester esterase BioH
RA 4,140,820 C→A 14.6% C50F (TGC→TTC)  Y75_RS20420 ← YhfL family protein
RA 4,199,333 A→G 26.3% H198R (CAC→CGC)  rpsD → 30S ribosomal protein S4
RA 4,248,243 G→T 9.8% G141G (GGC→GGA malF ← maltose ABC transporter permease MalF
RA 4,259,643 G→C 12.1% G138G (GGC→GGG plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase PlsB
RA 4,275,018 T→C 26.5% D815G (GAC→GGC)  uvrA ← excinuclease ABC subunit UvrA
RA 4,278,433 T→G 9.2% T67P (ACC→CCC)  Y75_RS21180 ← membrane protein
RA 4,290,120 T→A 11.5% Y281F (TAT→TTT)  acs ← acetate‑‑CoA ligase
MC JC 4,310,363 Δ1,199 bp 100% Y75_RS21310[alsK] Y75_RS21310, [alsK]
RA 4,323,045 T→C 11.0% E133G (GAG→GGG)  phnK ← phosphonate C‑P lyase system protein PhnK
RA 4,341,575 T→C 17.2% G45G (GGA→GGG adiC ← arginine/agmatine antiporter
RA 4,371,271 Δ2 bp 100% pseudogene (182‑183/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter
RA 4,372,300 A→G 30.0% G236G (GGT→GGC aspA ← aspartate ammonia‑lyase
RA 4,373,555 A→G 17.0% E71G (GAG→GGG)  fxsA → membrane protein FxsA
RA 4,425,872 A→G 14.8% S405G (AGT→GGT)  ulaA → PTS ascorbate transporter subunit IIC
RA 4,425,882 T→G 15.8% M408R (ATG→AGG)  ulaA → PTS ascorbate transporter subunit IIC
RA 4,468,554 T→G 7.6% H279P (CAC→CCC)  treC ← alpha,alpha‑phosphotrehalase
RA 4,486,479 T→C 8.5% E680G (GAG→GGG)  valS ← valine‑‑tRNA ligase
RA 4,535,841 T→G 10.3% N164T (AAC→ACC)  Y75_RS22465 ← M42 family metallopeptidase
RA 4,561,746 T→C 23.3% V25A (GTA→GCA)  iraD → anti‑adapter protein IraD
RA 4,583,511 A→G 33.1% C169C (TGT→TGC Y75_RS22715 ← 5‑methylcytosine‑specific restriction endonuclease subunit McrB
RA 4,595,570 A→C 14.5% G130G (GGT→GGG btsT ← pyruvate/proton symporter BtsT
RA 4,603,422 C→T 23.6% A234T (GCC→ACC)  opgB ← phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
RA 4,609,066 G→T 12.2% C76F (TGC→TTC)  bglJ → DNA‑binding transcriptional activator BglJ
RA 4,611,582 A→G 18.8% S267P (TCC→CCC)  rsmC ← 16S rRNA (guanine(1207)‑N(2))‑methyltransferase RsmC
RA 4,632,259 A→C 23.6% S89R (AGC→CGC)  nadR → multifunctional transcriptional regulator/nicotinamide‑nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1301041–1302370 1302407 38–1367 150 [0] [0] 184 Y75_RS06530–Y75_RS06530 Y75_RS06530,Y75_RS06530
* * ÷ NC_007779 1397873–1398957 1406267 7311–8395 67 [66] [0] 138 [Y75_RS07025]–[Y75_RS07065] [Y75_RS07025],Y75_RS07030,uspE,Y75_RS07040,ogt,abgT,Y75_RS07060,[Y75_RS07065]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 228016 =NA (NA)6 (0.040) 6/410 NT NA noncoding (2267/2930 nt) Y75_RS01035 23S ribosomal RNA
?NC_007779 228041 = NA (NA)noncoding (2292/2930 nt) Y75_RS01035 23S ribosomal RNA
* ? NC_007779 273180 =NA (NA)180 (1.110) 103/422 NT 100% intergenic (+3/+145) Y75_RS01330/Y75_RS01335 hypothetical protein/IS5‑like element IS5 family transposase
?NC_007779 701988 = 0 (0.000)coding (29/1221 nt) nagC DNA‑binding transcriptional regulator NagC
* ? NC_007779 = 3806977 (0.040)16 (0.100) 5/402 NT 70.7% coding (168/361 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 = 381063 NA (NA)coding (174/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 607256 =NA (NA)8 (0.060) 6/372 NT 5.8% intergenic (+45/‑32) Y75_RS03010/Y75_RS03015 type I toxin‑antitoxin system toxin HokE/IS4‑like element IS421 family transposase
?NC_007779 608549 = 149 (0.910)intergenic (+149/+133) Y75_RS03015/entD IS4‑like element IS421 family transposase/enterobactin synthase subunit EntD
* ? NC_007779 = 689467NA (NA)162 (0.990) 92/424 NT 100% intergenic (‑32/+298) Y75_RS03405/lnt IS5‑like element IS5 family transposase/apolipoprotein N‑acyltransferase
?NC_007779 = 701992 0 (0.000)coding (25/1221 nt) nagC DNA‑binding transcriptional regulator NagC
* ? NC_007779 1097383 =109 (0.670)9 (0.060) 4/418 NT 11.2% intergenic (+132/+242) Y75_RS05375/Y75_RS05385 hypothetical protein/RtT sRNA
?NC_007779 1097819 = 35 (0.220)noncoding (75/88 nt) Y75_RS05390 tRNA‑Ser
* ? NC_007779 1209367 =84 (0.510)105 (0.690) 69/392 NT 59.2% coding (290/630 nt) Y75_RS06020 hypothetical protein
?NC_007779 1211196 = 67 (0.440)intergenic (‑6/‑66) Y75_RS06035/pinE tail fiber protein/site‑specific DNA recombinase
* ? NC_007779 = 120938286 (0.530)109 (0.720) 69/392 NT 59.8% coding (305/630 nt) Y75_RS06020 hypothetical protein
?NC_007779 = 1211179 67 (0.440)coding (12/495 nt) Y75_RS06035 tail fiber protein
* ? NC_007779 1294773 =95 (0.580)5 (0.030) 5/424 NT 5.0% intergenic (‑274/‑331) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 2176983 = NA (NA)intergenic (‑3/‑32) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
* ? NC_007779 = 129477690 (0.550)8 (0.050) 7/424 NT 8.2% intergenic (‑277/‑328) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 1980209 = NA (NA)intergenic (‑298/+146) flhD/Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
* ? NC_007779 1294844 =4 (0.020)171 (1.050) 93/422 NT 97.7% intergenic (‑345/‑260) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 = 1398951 NA (NA)intergenic (+145/‑128) Y75_RS07025/Y75_RS07030 IS5‑like element IS5 family transposase/DUF2534 family protein
* ? NC_007779 1294844 =4 (0.020)41 (0.250) 34/422 NT 91.1% intergenic (‑345/‑260) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 2176984 = NA (NA)intergenic (‑4/‑31) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
* ? NC_007779 = 12948484 (0.020)130 (0.790) 81/424 NT 97.0% intergenic (‑349/‑256) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 1397758 = NA (NA)intergenic (‑122/‑32) ynaI/Y75_RS07025 low conductance mechanosensitive channel YnaI/IS5‑like element IS5 family transposase
* ? NC_007779 = 12948484 (0.020)66 (0.400) 52/424 NT 94.3% intergenic (‑349/‑256) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 1980209 = NA (NA)intergenic (‑298/+146) flhD/Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
* ? NC_007779 = 13010400 (0.000)116 (0.820)
+28 bp
74/368 NT 100% intergenic (+218/‑45) ychE/Y75_RS06530 NAAT family transporter YchE/IS3‑like element IS2 family transposase
?NC_007779 = 4311564 NA (NA)pseudogene (914/921 nt) alsK allose kinase
* ? NC_007779 1302408 =0 (0.000)162 (0.990) 90/424 NT 100% intergenic (+50/‑488) Y75_RS06530/oppA IS3‑like element IS2 family transposase/oligopeptide ABC transporter substrate‑binding protein OppA
?NC_007779 1397758 = NA (NA)intergenic (‑122/‑32) ynaI/Y75_RS07025 low conductance mechanosensitive channel YnaI/IS5‑like element IS5 family transposase
* ? NC_007779 = 1388812135 (0.830)9 (0.060) 7/412 NT 6.0% coding (379/1542 nt) tyrR transcriptional regulator TyrR
?NC_007779 = 1388962 151 (0.950)coding (529/1542 nt) tyrR transcriptional regulator TyrR
* ? NC_007779 = 1398952NA (NA)108 (0.660) 67/424 NT 65.9% intergenic (+146/‑127) Y75_RS07025/Y75_RS07030 IS5‑like element IS5 family transposase/DUF2534 family protein
?NC_007779 1909106 = 56 (0.340)coding (44/210 nt) cspE transcription antiterminator/RNA stability regulator CspE
* ? NC_007779 1406268 =0 (0.000)131 (0.800) 79/424 NT 100% coding (12/1311 nt) Y75_RS07065 p‑aminobenzoyl‑glutamate hydrolase subunit AbgA
?NC_007779 1980209 = NA (NA)intergenic (‑298/+146) flhD/Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
* ? NC_007779 1885208 =38 (0.230)105 (0.640) 67/424 NT 73.4% coding (307/1446 nt) Y75_RS09485 BCCT family transporter
?NC_007779 2411458 = NA (NA)intergenic (‑147/+15) lrhA/Y75_RS12035 transcriptional regulator LrhA/IS1‑like element IS1A family transposase
* ? NC_007779 = 188521542 (0.260)142 (0.870) 90/424 NT 77.2% coding (314/1446 nt) Y75_RS09485 BCCT family transporter
?NC_007779 = 2412225 NA (NA)intergenic (‑55/‑782) Y75_RS12035/Y75_RS12040 IS1‑like element IS1A family transposase/alanine transaminase AlaA
* ? NC_007779 = 190910957 (0.350)122 (0.750) 74/424 NT 68.2% coding (41/210 nt) cspE transcription antiterminator/RNA stability regulator CspE
?NC_007779 = 1981403 NA (NA)intergenic (‑32/‑487) Y75_RS09980/uspC IS5‑like element IS5 family transposase/universal stress protein UspC
* ? NC_007779 2063152 =102 (0.620)58 (0.360) 45/422 NT 36.4% intergenic (‑101/+1) cbl/nac HTH‑type transcriptional regulator Cbl/nitrogen assimilation transcriptional regulator
?NC_007779 2176984 = NA (NA)intergenic (‑4/‑31) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
* ? NC_007779 = 2063156103 (0.630)28 (0.170) 24/424 NT 21.4% coding (915/918 nt) nac nitrogen assimilation transcriptional regulator
?NC_007779 2103886 = NA (NA)pseudogene (1/354 nt) Y75_RS23870 glycosyltransferase family 2 protein
* ? NC_007779 2728361 =NA (NA)4 (0.020) 3/418 NT NA noncoding (1453/1554 nt) Y75_RS13580 16S ribosomal RNA
?NC_007779 2728548 = NA (NA)noncoding (1266/1554 nt) Y75_RS13580 16S ribosomal RNA
* ? NC_007779 = 2882429155 (0.950)8 (0.050) 5/408 NT 5.4% coding (366/1509 nt) casA type I‑E CRISPR‑associated protein Cse1/CasA
?NC_007779 = 2882536 130 (0.830)coding (259/1509 nt) casA type I‑E CRISPR‑associated protein Cse1/CasA
* ? NC_007779 3128803 =NA (NA)20 (0.120) 18/422 NT 13.6% coding (33/1137 nt) Y75_RS15525 hypothetical protein
?NC_007779 = 3132911 128 (0.780)coding (125/693 nt) Y75_RS15550 thymidylate kinase
* ? NC_007779 = 3129996NA (NA)17 (0.100) 16/424 NT 12.2% intergenic (+146/+1) Y75_RS15530/Y75_RS15535 IS5‑like element IS5 family transposase/lipopolysaccharide biosynthesis protein
?NC_007779 3132907 = 122 (0.750)coding (121/693 nt) Y75_RS15550 thymidylate kinase
* ? NC_007779 3261134 =NA (NA)57 (0.350) 40/424 NT 29.7% intergenic (‑60/‑32) tdcE/Y75_RS16205 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase/IS5‑like element IS5 family transposase
?NC_007779 = 3306581 135 (0.830)coding (1135/1890 nt) Y75_RS16445 ATP‑dependent RNA helicase DeaD
* ? NC_007779 = 3262327NA (NA)46 (0.280) 36/422 NT 25.4% intergenic (+145/+4) Y75_RS16205/tdcD IS5‑like element IS5 family transposase/propionate kinase
?NC_007779 3306577 = 136 (0.830)coding (1139/1890 nt) Y75_RS16445 ATP‑dependent RNA helicase DeaD
* ? NC_007779 3419810 =114 (0.700)8 (0.050) 4/408 NT 7.2% pseudogene (914/1025 nt) Y75_RS16995 amino acid ABC transporter substrate‑binding protein
?NC_007779 3419983 = 97 (0.620)intergenic (+62/‑6) Y75_RS16995/Y75_RS17000 amino acid ABC transporter substrate‑binding protein/amino acid ABC transporter permease
* ? NC_007779 3748316 =0 (0.000)145 (0.890) 82/424 NT 100% pseudogene (665/873 nt) tnaB low affinity tryptophan permease TnaB
?NC_007779 = 4056987 NA (NA)coding (391/417 nt) Y75_RS20060 hypothetical protein
* ? NC_007779 = 37483243 (0.020)156 (0.950) 90/424 NT 98.1% pseudogene (657/873 nt) tnaB low affinity tryptophan permease TnaB
?NC_007779 4056220 = NA (NA)intergenic (‑209/+15) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
* ? NC_007779 4056221 =NA (NA)160 (0.980) 81/422 NT 97.6% intergenic (‑210/+14) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
?NC_007779 4370787 = 4 (0.020)pseudogene (667/1304 nt) Y75_RS21600 anaerobic C4‑dicarboxylate transporter
* ? NC_007779 = 4056987NA (NA)194 (1.190) 110/424 NT 99.0% coding (391/417 nt) Y75_RS20060 hypothetical protein
?NC_007779 = 4370795 2 (0.010)pseudogene (659/1304 nt) Y75_RS21600 anaerobic C4‑dicarboxylate transporter
* ? NC_007779 4110945 =148 (0.900)11 (0.070) 10/404 NT 7.1% coding (124/402 nt) hslR ribosome‑associated heat shock protein Hsp15
?NC_007779 4111021 = 147 (0.940)coding (48/402 nt) hslR ribosome‑associated heat shock protein Hsp15