breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 14,116 A→G 6.3% intergenic (+37/‑52) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
RA 14,119 G→A 6.4% intergenic (+40/‑49) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
RA 14,124 T→A 5.7% intergenic (+45/‑44) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
RA 14,129 T→C 8.7% intergenic (+50/‑39) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
RA 14,132 C→T 8.7% intergenic (+53/‑36) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
RA 73,013 G→C 9.5% R503R (CGC→CGG thiP ← thiamine/thiamine pyrophosphate ABC transporter permease
RA 98,451 A→T 6.4% S17C (AGT→TGT)  ftsW → putative lipid II flippase; integral membrane protein; FtsZ ring stabilizer
RA 121,012 G→A 5.5% G180G (GGC→GGT aroP ← aromatic amino acid transporter
RA 155,248 A→G 5.0% I60T (ATA→ACA)  htrE ← putative outer membrane usher protein
RA 162,603 A→T 6.7% M167L (ATG→TTG)  hrpB → putative ATP‑dependent helicase
RA 163,418 A→T 6.6% K438N (AAA→AAT hrpB → putative ATP‑dependent helicase
RA 163,419 A→T 5.8% I439F (ATT→TTT)  hrpB → putative ATP‑dependent helicase
RA 167,291 G→A 5.2% intergenic (+27/‑193) mrcB → / → fhuA fused glycosyl transferase and transpeptidase/ferrichrome outer membrane transporter
RA 167,295 T→C 5.2% intergenic (+31/‑189) mrcB → / → fhuA fused glycosyl transferase and transpeptidase/ferrichrome outer membrane transporter
RA 192,579 A→T 5.9% *242L (TAA→TTA)  pyrH → uridylate kinase
RA 209,172 C→G 6.2% N184K (AAC→AAG accA → acetyl‑CoA carboxylase, carboxytransferase, alpha subunit
RA 222,958 Δ1 bp 100% coding (126/576 nt) gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 260,145 G→C 5.6% intergenic (‑45/‑243) phoE ← / → proB outer membrane phosphoporin protein E/gamma‑glutamate kinase
RA 289,256 G→C 8.9% intergenic (‑94/+45) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
RA 289,257 T→A 9.0% intergenic (‑95/+44) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
RA 458,742 T→A 5.6% intergenic (+42/‑146) clpX → / → lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
RA 475,971 C→G 6.2% intergenic (+20/+11) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 475,972 C→G 6.4% intergenic (+21/+10) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 478,750 C→T 5.4% intergenic (‑133/+31) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
RA 478,752 G→A 5.4% intergenic (‑135/+29) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
RA 478,757 T→C 5.4% intergenic (‑140/+24) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
RA 478,759 A→G 5.3% intergenic (‑142/+22) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
RA 595,519 T→A 6.0% intergenic (‑76/‑81) cusR ← / → cusC response regulator in two‑component regulatory system with CusS/copper/silver efflux system, outer membrane component
RA 595,520 T→A 5.1% intergenic (‑77/‑80) cusR ← / → cusC response regulator in two‑component regulatory system with CusS/copper/silver efflux system, outer membrane component
RA 645,069 G→A 6.7% intergenic (‑66/+48) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier
RA 645,073 T→C 5.9% intergenic (‑70/+44) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier
RA 645,074 T→C 5.7% intergenic (‑71/+43) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier
RA 691,791 G→A 6.8% intergenic (‑7/+83) ybeX ← / ← ybeY putative ion transport/ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor
RA 708,668 C→T 8.4% A112V (GCG→GTG)  chiP → chitoporin, uptake of chitosugars
RA 715,749 T→C 8.0% V113A (GTT→GCT)  ybfP → lipoprotein
RA 715,755 A→C 8.0% Q115P (CAG→CCG) ‡ ybfP → lipoprotein
RA 715,756 G→T 11.3% Q115H (CAG→CAT) ‡ ybfP → lipoprotein
RA 715,762 G→A 7.7% K117K (AAG→AAA ybfP → lipoprotein
RA 741,034 A→G 5.7% intergenic (+109/+41) phr → / ← dtpD deoxyribodipyrimidine photolyase, FAD‑binding/dipeptide and tripeptide permease D
RA 741,041 G→T 5.5% intergenic (+116/+34) phr → / ← dtpD deoxyribodipyrimidine photolyase, FAD‑binding/dipeptide and tripeptide permease D
RA 741,043 C→G 5.6% intergenic (+118/+32) phr → / ← dtpD deoxyribodipyrimidine photolyase, FAD‑binding/dipeptide and tripeptide permease D
RA 741,045 A→C 6.4% intergenic (+120/+30) phr → / ← dtpD deoxyribodipyrimidine photolyase, FAD‑binding/dipeptide and tripeptide permease D
RA 741,051 G→T 6.1% intergenic (+126/+24) phr → / ← dtpD deoxyribodipyrimidine photolyase, FAD‑binding/dipeptide and tripeptide permease D
RA 741,052 C→T 5.3% intergenic (+127/+23) phr → / ← dtpD deoxyribodipyrimidine photolyase, FAD‑binding/dipeptide and tripeptide permease D
RA 875,408 G→T 13.9% G25V (GGG→GTG)  yliF → putative membrane‑anchored diguanylate cyclase
RA 900,201 T→G 5.5% K125N (AAA→AAC artJ ← arginine ABC transporter periplasmic binding protein
RA 900,209 C→A 5.8% D123Y (GAT→TAT)  artJ ← arginine ABC transporter periplasmic binding protein
RA 959,667 A→C 7.5% T286P (ACC→CCC)  aroA → 5‑enolpyruvylshikimate‑3‑phosphate synthetase
RA 1,017,074 A→G 5.7% L409P (CTG→CCG)  ycbZ ← putative peptidase
RA 1,058,324 C→A 5.7% H81N (CAT→AAT)  torC → trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
RA 1,058,696 A→G 5.7% T205A (ACG→GCG)  torC → trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
RA 1,060,382 A→T 5.4% Q376L (CAG→CTG)  torA → trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit
RA 1,060,388 T→C 5.5% M378T (ATG→ACG)  torA → trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit
RA 1,145,939 A→G 7.1% intergenic (+40/+72) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
RA 1,145,940 A→C 8.2% intergenic (+41/+71) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
RA 1,145,946 T→A 6.2% intergenic (+47/+65) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
RA 1,145,947 T→A 6.2% intergenic (+48/+64) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
RA 1,145,954 C→T 5.7% intergenic (+55/+57) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
RA 1,146,736 A→G 6.1% noncoding (148/169 nt) psrD → novel Novel sRNA, function unknown
RA 1,146,740 C→G 6.1% noncoding (152/169 nt) psrD → novel Novel sRNA, function unknown
RA 1,146,744 C→T 6.5% noncoding (156/169 nt) psrD → novel Novel sRNA, function unknown
RA 1,314,682 A→T 7.3% intergenic (+24/+36) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
RA 1,314,683 T→A 7.3% intergenic (+25/+35) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
RA 1,314,684 A→T 7.1% intergenic (+26/+34) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
RA 1,357,227 A→G 5.3% intergenic (‑117/+196) sapA ← / ← ymjA antimicrobial peptide transport ABC transporter periplasmic binding protein/DUF2543 family protein
RA 1,357,230 T→A 5.8% intergenic (‑120/+193) sapA ← / ← ymjA antimicrobial peptide transport ABC transporter periplasmic binding protein/DUF2543 family protein
RA 1,357,233 C→T 5.4% intergenic (‑123/+190) sapA ← / ← ymjA antimicrobial peptide transport ABC transporter periplasmic binding protein/DUF2543 family protein
RA 1,357,236 T→C 5.2% intergenic (‑126/+187) sapA ← / ← ymjA antimicrobial peptide transport ABC transporter periplasmic binding protein/DUF2543 family protein
RA 1,393,995 T→C 8.3% Y257H (TAC→CAC)  mppA → murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
RA 1,409,134 T→A 6.3% noncoding (6/122 nt) fnrS → FNR‑activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq‑dependent; mediates negative FNR regulation
RA 1,409,145 T→C 6.2% noncoding (17/122 nt) fnrS → FNR‑activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq‑dependent; mediates negative FNR regulation
RA 1,409,156 G→A 6.7% noncoding (28/122 nt) fnrS → FNR‑activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq‑dependent; mediates negative FNR regulation
RA 1,441,833 G→C 6.9% intergenic (‑90/+21) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
RA 1,441,837 G→C 5.5% intergenic (‑94/+17) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
RA 1,466,201 T→C 5.9% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,541,626 Δ1 bp 10.3% coding (965/3741 nt) narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,552,191 C→A 21.7% intergenic (+200/+207) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
RA 1,552,263 G→T 9.3% intergenic (+272/+135) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
RA 1,652,362 A→G 5.5% pseudogene (812/1158 nt) intQ → pseudogene, Qin prophage; phage integrase family;Phage or Prophage Related
RA 1,652,363 A→G 5.5% pseudogene (813/1158 nt) intQ → pseudogene, Qin prophage; phage integrase family;Phage or Prophage Related
RA 1,652,368 C→T 5.6% pseudogene (818/1158 nt) intQ → pseudogene, Qin prophage; phage integrase family;Phage or Prophage Related
RA 1,652,369 C→T 5.6% pseudogene (819/1158 nt) intQ → pseudogene, Qin prophage; phage integrase family;Phage or Prophage Related
RA 1,660,200 C→G 6.8% A711G (GCC→GGC)  ynfE → putative selenate reductase, periplasmic
RA 1,660,206 G→T 6.7% R713L (CGT→CTT)  ynfE → putative selenate reductase, periplasmic
RA 1,679,398 T→A 7.3% intergenic (+27/‑159) ydgH → / → ydgI DUF1471 family periplasmic protein/putative arginine/ornithine antiporter transporter
RA 1,679,400 G→C 7.2% intergenic (+29/‑157) ydgH → / → ydgI DUF1471 family periplasmic protein/putative arginine/ornithine antiporter transporter
RA 1,679,402 T→A 6.0% intergenic (+31/‑155) ydgH → / → ydgI DUF1471 family periplasmic protein/putative arginine/ornithine antiporter transporter
RA 1,703,251 A→T 7.5% intergenic (+17/+17) add → / ← ydgJ adenosine deaminase/putative oxidoreductase
RA 1,706,114 G→A 8.0% V116V (GTG→GTA rsxA → SoxR iron‑sulfur cluster reduction factor component; inner membrane protein of electron transport complex
RA 1,765,157 T→A 11.2% intergenic (‑35/+65) ydiH ← / ← menI uncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
RA 1,765,160 C→G 9.1% intergenic (‑38/+62) ydiH ← / ← menI uncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
RA 1,765,161 A→G 9.1% intergenic (‑39/+61) ydiH ← / ← menI uncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
RA 1,765,162 C→T 9.2% intergenic (‑40/+60) ydiH ← / ← menI uncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
RA 1,765,163 C→G 9.1% intergenic (‑41/+59) ydiH ← / ← menI uncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
RA 1,765,166 T→A 8.5% intergenic (‑44/+56) ydiH ← / ← menI uncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
RA 1,865,682 G→C 5.6% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
RA 1,944,744 T→C 5.9% S205G (AGT→GGT)  ruvB ← ATP‑dependent DNA helicase, component of RuvABC resolvasome
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 1,988,051 A→T 11.7% intergenic (‑92/‑171) azuC ← / → yecR acid‑inducible small membrane‑associated protein/lipoprotein, function unknown
RA 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein
RA 2,078,297 Δ1 bp 7.4% intergenic (+190/+252) yeeW → / ← yoeD CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,078,301 A→T 7.6% intergenic (+194/+248) yeeW → / ← yoeD CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,078,304 A→T 7.7% intergenic (+197/+245) yeeW → / ← yoeD CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,078,306:1 +G 7.8% intergenic (+199/+243) yeeW → / ← yoeD CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,122,676 T→A 8.8% M102L (ATG→TTG)  cpsG ← phosphomannomutase
RA 2,125,654 T→C 5.3% Q31Q (CAA→CAG wcaI ← putative glycosyl transferase
RA 2,135,866 A→G 5.7% L78P (CTG→CCG)  wzb ← colanic acid production protein‑tyrosine‑phosphatase; Wzc‑P dephosphorylase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,354,885 G→A 5.5% A207A (GCG→GCA glpB → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit
RA 2,373,177 G→A 8.2% G96S (GGC→AGC)  arnF → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
RA 2,373,180 T→C 5.9% W97R (TGG→CGG)  arnF → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
JC JC 2,375,828 IS186 (+) +4 bp 100% coding (132‑135/963 nt) menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,395,812 G→C 6.4% L29V (CTG→GTG)  nuoJ ← NADH:ubiquinone oxidoreductase, membrane subunit J
RA 2,399,876 G→A 5.8% T97I (ACC→ATC)  nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,399,878 C→G 5.6% M96I (ATG→ATC) ‡ nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,399,880 T→C 5.6% M96V (ATG→GTG) ‡ nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,425,289 C→T 6.3% G210S (GGT→AGT)  hisQ ← histidine ABC transporter permease
RA 2,462,677 A→T 6.3% intergenic (+31/‑335) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,462,678 T→A 6.6% intergenic (+32/‑334) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,462,679 A→T 6.1% intergenic (+33/‑333) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,504,799 T→A 6.1% K250M (AAG→ATG) ‡ ypdE ← aminopeptidase
RA 2,504,800 T→A 6.1% K250* (AAG→TAG) ‡ ypdE ← aminopeptidase
RA 2,547,696 G→A 7.2% A342T (GCA→ACA)  murP → N‑acetylmuramic acid permease, EIIBC component, PTS system
RA 2,592,989 A→G 7.1% intergenic (+27/+83) ypfN → / ← ypfH putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate
RA 2,592,990 A→C 7.3% intergenic (+28/+82) ypfN → / ← ypfH putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate
RA 2,593,003 G→T 8.4% intergenic (+41/+69) ypfN → / ← ypfH putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate
RA 2,593,004 C→T 7.6% intergenic (+42/+68) ypfN → / ← ypfH putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate
RA 2,652,132 C→G 7.0% V52V (GTG→GTC yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,657,426 G→A 5.8% L504L (CTG→TTG)  hscA ← DnaK‑like molecular chaperone specific for IscU
RA 2,657,433 A→T 5.6% S501S (TCT→TCA hscA ← DnaK‑like molecular chaperone specific for IscU
RA 2,657,440 T→C 5.8% K499R (AAA→AGA)  hscA ← DnaK‑like molecular chaperone specific for IscU
RA 2,673,783 G→A 10.2% intergenic (+15/+33) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family
RA 2,673,784 C→A 9.5% intergenic (+16/+32) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family
RA 2,673,790 G→T 9.1% intergenic (+22/+26) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family
RA 2,673,791 A→C 10.7% intergenic (+23/+25) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family
RA 2,673,797 T→G 11.4% intergenic (+29/+19) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family
RA 2,673,798 T→C 9.6% intergenic (+30/+18) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family
RA 2,761,623 C→A 6.7% V640L (GTA→TTA)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
RA 2,775,502 T→C 5.7% intergenic (+481/‑43) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
RA 2,775,503 C→T 5.7% intergenic (+482/‑42) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
RA 2,775,504 A→G 5.7% intergenic (+483/‑41) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
RA 2,775,505 G→A 5.8% intergenic (+484/‑40) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
RA 2,834,232 C→G 6.9% T107T (ACC→ACG norW → NADH:flavorubredoxin oxidoreductase
RA 2,834,234 G→T 7.1% G108V (GGT→GTT)  norW → NADH:flavorubredoxin oxidoreductase
RA 2,839,326 C→G 8.4% intergenic (‑62/‑198) ascG ← / → ascF asc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
RA 2,863,772 G→A 5.6% R60R (CGG→CGA ygbL → putative class II aldolase
RA 2,863,777 C→G 5.6% S62C (TCC→TGC) ‡ ygbL → putative class II aldolase
RA 2,863,778 C→G 5.6% S62S (TCC→TCG) ‡ ygbL → putative class II aldolase
RA 2,863,783 T→C 5.5% V64A (GTC→GCC)  ygbL → putative class II aldolase
RA 2,866,487 G→C 5.4% intergenic (+22/+72) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor
RA 2,866,488 G→C 5.4% intergenic (+23/+71) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor
RA 2,904,212 G→A 100% intergenic (+838/+535) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 2,919,557 A→G 7.1% L397P (CTG→CCG)  gudX ← glucarate dehydratase‑related protein, substrate unknown
RA 2,919,561 C→T 7.5% G396S (GGT→AGT)  gudX ← glucarate dehydratase‑related protein, substrate unknown
RA 2,967,529 A→C 14.1% G302G (GGT→GGG ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 3,020,511 C→G 6.2% intergenic (+22/‑29) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase
RA 3,057,140 A→G 6.6% intergenic (+152/+38) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
RA 3,057,145 G→C 6.2% intergenic (+157/+33) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
RA 3,057,150 C→T 6.8% intergenic (+162/+28) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
RA 3,059,704 A→T 8.3% intergenic (‑3/‑49) yqfE ← / → argP pseudogene, LysR family/transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,079,856 C→G 6.9% A594P (GCT→CCT)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,080,449 A→G 6.0% I396T (ATC→ACC)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,146,761 T→G 8.1% intergenic (‑24/+95) yghW ← / ← yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family
RA 3,146,762 G→A 8.3% intergenic (‑25/+94) yghW ← / ← yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family
RA 3,146,763 T→C 8.4% intergenic (‑26/+93) yghW ← / ← yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family
RA 3,146,764 C→A 9.8% intergenic (‑27/+92) yghW ← / ← yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family
RA 3,171,852 C→T 6.3% intergenic (+19/+27) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,171,857 A→T 6.5% intergenic (+24/+22) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,171,858 A→T 6.5% intergenic (+25/+21) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,171,863 A→G 6.6% intergenic (+30/+16) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,328,183 T→A 5.4% intergenic (+100/+56) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
RA 3,368,219 G→A 5.1% T131T (ACG→ACA yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,368,220 C→G 5.8% L132V (CTG→GTG)  yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,376,265 G→C 6.7% intergenic (+29/+14) dcuD → / ← sspB putative transporter/ClpXP protease specificity enhancing factor
RA 3,390,661 T→A 6.2% K456N (AAA→AAT tldD ← putative peptidase
RA 3,423,396 T→G 7.0% intergenic (+202/+27) yhdZ → / ← rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
RA 3,439,588 G→C 5.1% intergenic (‑79/+28) yhdN ← / ← rplQ DUF1992 family protein/50S ribosomal subunit protein L17
RA 3,539,816 T→A 5.1% intergenic (+110/‑347) yhgF → / → feoA putative transcriptional accessory protein/ferrous iron transporter, protein A
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,570,714:1 +T 6.1% coding (606/1974 nt) glgX ← glycogen debranching enzyme
RA 3,570,719 C→G 5.3% V201L (GTG→CTG)  glgX ← glycogen debranching enzyme
RA 3,570,724 Δ1 bp 5.8% coding (596/1974 nt) glgX ← glycogen debranching enzyme
RA 3,644,343 A→T 5.8% F280L (TTT→TTA prlC ← oligopeptidase A
RA 3,781,101 A→C 13.7% intergenic (+84/‑114) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,103 A→C 16.7% intergenic (+86/‑112) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,832,797 T→G 6.8% N581H (AAC→CAC)  yicI ← putative alpha‑glucosidase
JC JC 3,890,075 IS5 (–) +4 bp 100% coding (1346‑1349/1416 nt) tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,891,502 C→G 5.1% intergenic (+19/‑113) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,504 T→A 7.4% intergenic (+21/‑111) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,506 C→G 5.5% intergenic (+23/‑109) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,901,308 A→G 5.3% W305R (TGG→CGG)  bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,908,428 C→G 6.0% intergenic (‑62/+121) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease
RA 3,908,435 T→G 7.1% intergenic (‑69/+114) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease
RA 3,908,443 C→G 6.6% intergenic (‑77/+106) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease
RA 3,965,065 G→A 6.0% A189V (GCA→GTA)  rhlB ← ATP‑dependent RNA helicase
RA 3,969,429 A→C 6.5% K133N (AAA→AAC wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
RA 3,969,435 T→A 6.1% D135E (GAT→GAA wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
RA 3,969,436 G→T 6.3% A136S (GCG→TCG)  wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
RA 3,969,441 G→T 6.4% A137A (GCG→GCT wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
RA 3,990,679 T→G 7.2% S30R (AGC→CGC)  hemC ← hydroxymethylbilane synthase
RA 4,023,526 T→G 7.5% intergenic (+14/‑28) tatC → / → tatD TatABCE protein translocation system subunit/quality control of Tat‑exported FeS proteins; Mg‑dependent cytoplasmic DNase
RA 4,051,259 G→T 8.1% noncoding (224/245 nt) csrC → CsrC sRNA sequesters CsrA, a carbon flux regulator
RA 4,051,262 A→C 7.6% noncoding (227/245 nt) csrC → CsrC sRNA sequesters CsrA, a carbon flux regulator
RA 4,121,959 T→G 8.2% N110T (AAC→ACC)  hslV ← peptidase component of the HslUV protease
RA 4,179,562 C→T 5.6% G228G (GGC→GGT rplA → 50S ribosomal subunit protein L1
RA 4,189,448 C→T 57.3% Q1367* (CAG→TAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,189,605 T→C 5.1% intergenic (+32/‑181) rpoC → / → yjaZ RNA polymerase, beta prime subunit/stationary phase growth adaptation protein
RA 4,189,606 T→G 5.0% intergenic (+33/‑180) rpoC → / → yjaZ RNA polymerase, beta prime subunit/stationary phase growth adaptation protein
RA 4,189,607 C→A 6.0% intergenic (+34/‑179) rpoC → / → yjaZ RNA polymerase, beta prime subunit/stationary phase growth adaptation protein
RA 4,189,608 G→A 5.3% intergenic (+35/‑178) rpoC → / → yjaZ RNA polymerase, beta prime subunit/stationary phase growth adaptation protein
RA 4,249,431 T→A 5.9% intergenic (+120/‑123) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,291,513 T→C 6.4% M1A (GTG→GCG) † nrfE → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE
RA 4,296,060 C→T 54.6% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,297,884 A→G 6.1% Y495H (TAC→CAC)  fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit
RA 4,325,948 A→G 6.6% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,353,171 C→G 5.2% intergenic (+90/+29) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
RA 4,353,172 C→G 5.2% intergenic (+91/+28) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
RA 4,353,173 T→C 5.2% intergenic (+92/+27) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
RA 4,362,578 G→T 50.4% noncoding (49/76 nt) pheU ← tRNA‑Phe
RA 4,365,452 G→T 8.8% intergenic (‑96/+20) cutA ← / ← dcuA divalent‑cation tolerance protein, copper sensitivity/C4‑dicarboxylate antiporter
RA 4,365,453 A→C 9.3% intergenic (‑97/+19) cutA ← / ← dcuA divalent‑cation tolerance protein, copper sensitivity/C4‑dicarboxylate antiporter
RA 4,366,854 T→G 7.9% intergenic (‑81/+37) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,366,855 C→A 7.5% intergenic (‑82/+36) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,400,138 A→C 7.4% D296A (GAC→GCC)  miaA → delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase
RA 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
RA 4,414,243 A→T 5.1% intergenic (+52/‑32) yjfC → / → aidB ATP‑Grasp family ATPase/DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
RA 4,414,249 G→C 5.1% intergenic (+58/‑26) yjfC → / → aidB ATP‑Grasp family ATPase/DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
RA 4,457,256 T→G 5.3% intergenic (+98/+58) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
RA 4,484,461 A→C 7.8% A497A (GCT→GCG pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
RA 4,564,315 G→A 6.2% G125G (GGC→GGT yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase
RA 4,606,118 G→A 14.6% noncoding (48/87 nt) leuV ← tRNA‑Leu
JC JC 4,640,551 IS1 (–) +9 bp 100% intergenic (+9/‑383) yjjY → / → yjtD uncharacterized protein/putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 772987 774199 1213 26 [25] [25] 26 [cydA]–[cydX] [cydA],cydB,[cydX]
* * ÷ NC_000913 1037679 1039317 1639 26 [25] [24] 26 cbdA–[cbdB] cbdA,[cbdB]
* * ÷ NC_000913 1166059 1167414 1356 27 [25] [25] 26 ndh ndh
* * ÷ NC_000913 3423744–3424234 3424578–3424239 6–835 27 [25] [25] 26 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 16653NA (NA)4 (0.060) 4/262 NT NA noncoding (1267/1345 nt) IS186 repeat region
?NC_000913 = 16691 NA (NA)noncoding (1305/1345 nt) IS186 repeat region
* ? NC_000913 272886 =73 (1.070)4 (0.060) 4/266 NT 5.1% pseudogene (40/1108 nt) ykfC CP4‑6 prophage; conserved protein;Phage or Prophage Related
?NC_000913 272926 = 79 (1.200)pseudogene (80/1108 nt) ykfC CP4‑6 prophage; conserved protein;Phage or Prophage Related
* ? NC_000913 274347 =NA (NA)5 (0.080) 3/262 NT NA noncoding (803/1195 nt) IS5 repeat region
?NC_000913 274410 = NA (NA)noncoding (740/1195 nt) IS5 repeat region
* ? NC_000913 274384 =NA (NA)4 (0.060) 3/270 NT NA noncoding (766/1195 nt) IS5 repeat region
?NC_000913 274422 = NA (NA)noncoding (728/1195 nt) IS5 repeat region
* ? NC_000913 = 30509050 (0.730)3 (0.050) 3/268 NT 5.8% coding (116/711 nt) ecpE ECP production pilus chaperone
?NC_000913 = 305117 49 (0.740)coding (89/711 nt) ecpE ECP production pilus chaperone
* ? NC_000913 354606 =NA (NA)4 (0.060) 4/262 NT 5.0% noncoding (8/171 nt) REP26 (repetitive extragenic palindromic) element; contains 4 REP sequences REP26 (repetitive extragenic palindromic) element; contains 4 REP sequences
?NC_000913 354654 = 76 (1.170)noncoding (56/171 nt) REP26 (repetitive extragenic palindromic) element; contains 4 REP sequences REP26 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? NC_000913 = 381669NA (NA)6 (0.090) 6/262 NT NA noncoding (410/1331 nt) IS2 repeat region
?NC_000913 = 381695 NA (NA)noncoding (436/1331 nt) IS2 repeat region
* ? NC_000913 = 67726383 (1.210)5 (0.080) 5/262 NT 5.8% coding (553/708 nt) ybeR uncharacterized protein
?NC_000913 = 677272 83 (1.280)coding (562/708 nt) ybeR uncharacterized protein
* ? NC_000913 781369 =43 (0.630)15 (0.220)
+GAG
6/270 NT 35.0% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 14 (0.200)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 120186766 (0.960)4 (0.060) 3/266 NT 5.7% coding (218/309 nt) ymfJ e14 prophage; uncharacterized protein
?NC_000913 = 1201905 69 (1.050)coding (180/309 nt) ymfJ e14 prophage; uncharacterized protein
* ? NC_000913 1207790 =14 (0.200)24 (0.400) 23/244 NT 53.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 30 (0.500)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780515 (0.220)22 (0.360) 16/244 NT 50.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 30 (0.500)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)70 (1.050) 63/268 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 138786844 (0.640)4 (0.060) 4/270 NT 8.4% coding (1149/1542 nt) tyrR aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase
?NC_000913 = 1387892 44 (0.660)coding (1173/1542 nt) tyrR aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase
* ? NC_000913 1654352 =59 (0.860)3 (0.050) 3/264 NT 5.2% coding (790/1215 nt) rspA bifunctional D‑altronate/D‑mannonate dehydratase
?NC_000913 1654370 = 52 (0.790)coding (772/1215 nt) rspA bifunctional D‑altronate/D‑mannonate dehydratase
* ? NC_000913 2278525 =67 (0.980)4 (0.060) 4/268 NT 6.0% intergenic (‑288/+45) yejG/bcr uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
?NC_000913 2278565 = 61 (0.920)intergenic (‑328/+5) yejG/bcr uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
* ? NC_000913 = 295309166 (0.960)6 (0.090) 5/264 NT 8.6% coding (2913/3543 nt) recB exonuclease V (RecBCD complex), beta subunit
?NC_000913 = 2953105 65 (0.990)coding (2899/3543 nt) recB exonuclease V (RecBCD complex), beta subunit
* ? NC_000913 3423318 =97 (1.420)5 (0.080) 4/258 NT 5.4% intergenic (+124/+105) yhdZ/rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
?NC_000913 3423341 = 84 (1.310)intergenic (+147/+82) yhdZ/rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
* ? NC_000913 3473464 =70 (1.020)4 (0.060) 4/266 NT 5.5% coding (51/2115 nt) fusA protein chain elongation factor EF‑G, GTP‑binding
?NC_000913 3473495 = 71 (1.080)coding (20/2115 nt) fusA protein chain elongation factor EF‑G, GTP‑binding
* ? NC_000913 3591746 =74 (1.080)4 (0.060) 3/268 NT 5.5% coding (580/1317 nt) ugpB sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein
?NC_000913 3591824 = 66 (0.990)coding (502/1317 nt) ugpB sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein
* ? NC_000913 = 3620122NA (NA)3 (0.040)
+GG
3/272 NT 100% coding (931/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3620144 0 (0.000)coding (953/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 3620220NA (NA)3 (0.050) 3/264 NT 100% coding (1029/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3620255 0 (0.000)coding (1064/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 4184513 =68 (0.990)4 (0.060) 3/256 NT 6.0% coding (3269/4029 nt) rpoB RNA polymerase, beta subunit
?NC_000913 4184558 = 62 (0.980)coding (3314/4029 nt) rpoB RNA polymerase, beta subunit
* ? NC_000913 4246038 =54 (0.790)3 (0.050) 3/264 NT 5.7% coding (382/1191 nt) malE maltose transporter subunit
?NC_000913 4246058 = 47 (0.720)coding (362/1191 nt) malE maltose transporter subunit
* ? NC_000913 4390982 =64 (0.930)4 (0.060) 3/266 NT 6.3% coding (528/1053 nt) rsgA ribosome small subunit‑dependent GTPase A
?NC_000913 4391021 = 58 (0.880)coding (489/1053 nt) rsgA ribosome small subunit‑dependent GTPase A
* ? NC_000913 4636324 =51 (0.740)4 (0.060) 3/262 NT 7.4% coding (318/690 nt) creB response regulator in two‑component regulatory system with CreC
?NC_000913 4636375 = 52 (0.800)coding (369/690 nt) creB response regulator in two‑component regulatory system with CreC
* ? NC_000913 = 463633054 (0.790)3 (0.050) 3/262 NT 5.5% coding (324/690 nt) creB response regulator in two‑component regulatory system with CreC
?NC_000913 = 4636367 52 (0.800)coding (361/690 nt) creB response regulator in two‑component regulatory system with CreC