breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 69,561 A→G V163A (GTG→GCG)  araB ← ribulokinase
RA 80,258 A→G F203L (TTT→CTT)  leuC ← 3‑isopropylmalate dehydratase subunit LeuC
RA 128,019 A→G E36E (GAA→GAG lpd → lipoamide dehydrogenase
RA 128,203 A→G T98A (ACC→GCC)  lpd → lipoamide dehydrogenase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 316,398 A→G H271R (CAC→CGC)  insF‑1 → IS3 element protein InsF
RA 327,781 Δ1 bp coding (953/1473 nt) betB ← betaine aldehyde dehydrogenase
RA 332,895 A→G Q175Q (CAA→CAG pdeL → DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 427,568 A→G K94K (AAA→AAG yajC → Sec translocon accessory complex subunit YajC
RA 501,257 T→C V378A (GTA→GCA)  gsk → inosine/guanosine kinase
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 727,043 T→C intergenic (‑7/+16) kdpB ← / ← kdpA K(+) transporting P‑type ATPase subunit KdpB/K(+) transporting P‑type ATPase subunit KdpA
RA 734,682 A→G pseudogene (463/1434 nt) ybfO → conserved protein, rhs‑like YbfO
RA 735,131 G→A pseudogene (912/1434 nt) ybfO → conserved protein, rhs‑like YbfO
RA 759,856 T→C V384A (GTA→GCA)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 775,617 C→T A155A (GCC→GCT tolQ → Tol‑Pal system protein TolQ
RA 793,126 T→C E221E (GAA→GAG modF ← ABC family protein ModF
RA 798,196 A→G V79A (GTG→GCG)  ybhA ← pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase
RA 832,676 A→G V92A (GTC→GCC)  ybiA ← N‑glycosidase YbiA
RA 839,586 T→C Q649R (CAG→CGG)  fiu ← putative iron siderophore outer membrane transporter
RA 843,979 A→G L501P (CTA→CCA)  ybiO ← moderate conductance mechanosensitive channel
RA 863,358 G→A L52L (CTG→TTG)  ybiY ← putative pyruvate formate‑lyase activating enzyme YbiY
RA 868,422 (C)8→7 coding (903/1872 nt) gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 888,876 A→G V315A (GTA→GCA)  ybjL ← putative transport protein YbjL
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 911,132 A→G intergenic (‑83/+50) poxB ← / ← hcr pyruvate oxidase/NADH oxidoreductase
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 969,920 C→T R178W (CGG→TGG)  ycaQ → winged helix DNA‑binding domain‑containing protein YcaQ
RA 972,057 A→G A102A (GCA→GCG ycbJ → putative phosphotransferase YcbJ
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,077,756 C→T R376H (CGC→CAC)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,097,531 (A)8→7 intergenic (+702/+34) ycdU → / ← serX uncharacterized protein YcdU/tRNA‑Ser
RA 1,114,758 A→G Y245H (TAT→CAT)  mdtG ← efflux pump MdtG
RA 1,127,027 C→T A74A (GCC→GCT yceM → putative oxidoreductase YceM
RA 1,136,183 A→G Y207C (TAC→TGC)  flgH → flagellar L‑ring protein
RA 1,138,609 G→A A78T (GCG→ACG)  flgK → flagellar hook‑filament junction protein 1
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,266,725 A→G D211G (GAC→GGC)  prmC → protein‑(glutamine‑N(5)) methyltransferase
RA 1,270,109 T→G intergenic (+186/+129) rdlB → / ← ldrC small regulatory antisense RNA RdlB/small toxic polypeptide LdrC
RA 1,270,545 G→T intergenic (+85/+204) rdlC → / ← chaA small regulatory antisense RNA RdlC/Na(+)/K(+):H(+) antiporter ChaA
RA 1,270,583 G→A intergenic (+123/+166) rdlC → / ← chaA small regulatory antisense RNA RdlC/Na(+)/K(+):H(+) antiporter ChaA
RA 1,318,571 (C)6→7 coding (1218/1362 nt) trpC ← fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase
RA 1,366,662 A→G A371A (GCA→GCG puuE → 4‑aminobutyrate aminotransferase PuuE
RA 1,380,122 A→G T747A (ACC→GCC)  ycjT → kojibiose phosphorylase
RA 1,406,145 T→C I56T (ATC→ACC)  smrA → DNA endonuclease SmrA
RA 1,408,305 A→G T86A (ACG→GCG)  zntB → Zn(2(+)):H(+) symporter
RA 1,453,860 T→C intergenic (‑218/‑67) paaZ ← / → paaA oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase/phenylacetyl‑CoA 1,2‑epoxidase, monooxygenase subunit
RA 1,456,070 T→C V49A (GTG→GCG)  paaD → phenylacetate degradation protein
RA 1,482,152 T→C V415A (GTG→GCG)  ynbD → putative phosphatase
RA 1,493,424 C→T Q319* (CAA→TAA)  trg → methyl‑accepting chemotaxis protein Trg
RA 1,510,051 T→C S17P (TCG→CCG)  ydcR → fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
RA 1,513,875 T→C G15G (GGT→GGC ydcU → putative ABC transporter membrane subunit YdcU
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,564,981 T→C E251E (GAA→GAG dosP ← oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
RA 1,568,811 A→G S5P (TCC→CCC)  yddW ← putative lipoprotein YddW
RA 1,568,865 A→G intergenic (‑42/+89) yddW ← / ← gadC putative lipoprotein YddW/L‑glutamate:4‑aminobutyrate antiporter
RA 1,585,936 T→C E184G (GAA→GGA)  ydeP ← putative oxidoreductase YdeP
RA 1,605,416 G→A E122E (GAG→GAA lsrB → Autoinducer‑2 ABC transporter periplasmic binding protein
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,703,904 A→G G135G (GGT→GGC ydgJ ← putative oxidoreductase YdgJ
RA 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
RA 1,720,255 T→C V127A (GTA→GCA)  slyB → outer membrane lipoprotein SlyB
RA 1,800,084 T→C intergenic (‑85/+12) rpmI ← / ← infC 50S ribosomal subunit protein L35/translation initiation factor IF‑3
RA 1,831,878 A→T D35E (GAT→GAA astC ← succinylornithine transaminase
RA 1,834,771 T→C V219A (GTT→GCT)  ydjY → 4Fe‑4S ferredoxin‑type domain‑containing protein YdjY
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,903,091 A→G T4A (ACC→GCC)  manY → mannose‑specific PTS enzyme IIC component
RA 1,905,116 A→G I106V (ATT→GTT)  yobD → conserved inner membrane protein YobD
RA 1,923,432 T→A D92V (GAT→GTT)  yebY ← DUF2511 domain‑containing protein YebY
RA 1,933,615 T→C V330V (GTA→GTG edd ← phosphogluconate dehydratase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,033,994 (T)8→9 intergenic (+255/‑57) rseX → / → yedS small regulatory RNA RseX/putative outer membrane protein YedS, N‑terminal fragment
RA 2,041,357 A→G intergenic (+239/‑18) msrQ → / → zinT periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit/metal‑binding protein ZinT
RA 2,145,623 T→C S786S (AGT→AGC dgcE → putative diguanylate cyclase DgcE
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,195,760 G→A V487V (GTG→GTA metG → methionine‑‑tRNA ligase
RA 2,288,561 (C)7→6 pseudogene (354/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,296,418 T→C T203A (ACC→GCC)  ccmB ← heme trafficking system membrane protein CcmB
RA 2,304,981 +C intergenic (+588/+127) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
RA 2,377,184 T→C T137A (ACA→GCA)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,507,458 T→C N146S (AAC→AGC)  fryC ← putative PTS enzyme IIC component FryC
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,538,274 A→G T176A (ACT→GCT)  yfeS → conserved protein YfeS
RA 2,553,560 (T)6→5 coding (336/900 nt) hemF → coproporphyrinogen III oxidase
RA 2,559,890 T→C S345P (TCA→CCA)  intZ → CPZ‑55 prophage; putative phage integrase IntZ
RA 2,596,660 A→G G26G (GGT→GGC ypfJ ← uncharacterized protein YpfJ
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
JC 2,734,543 (C)8→11 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,770,027 T→C S109P (TCT→CCT)  yfjR → CP4‑57 prophage; putative DNA‑binding transcriptional regulator
RA 2,773,481 A→G E55G (GAA→GGA)  yfjW → CP4‑57 prophage; uncharacterized protein YfjW
RA 2,798,184 A→G G104G (GGT→GGC stpA ← DNA‑binding transcriptional repressor StpA with RNA chaperone activity
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,819,064 T→C N28S (AAC→AGC)  csrA ← carbon storage regulator
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,875,459 C→T G291R (GGG→AGG)  cysD ← sulfate adenylyltransferase subunit 2
RA 2,895,366 T→C Q138Q (CAA→CAG ygcQ ← putative flavoprotein
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,972,909 (C)6→7 coding (954/1194 nt) lplT ← lysophospholipid transporter
RA 2,983,384 A→G G247G (GGT→GGC kduI ← 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
RA 2,998,609 G→A H74Y (CAC→TAC)  ygeQ ← protein YgeQ
RA 3,030,784 C→T A50T (GCT→ACT)  ygfT ← fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit
RA 3,060,772 (T)8→7 intergenic (+126/‑78) argP → / → scpA DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,123,295 G→A R171W (CGG→TGG)  glcB ← malate synthase G
RA 3,142,370 A→G L207P (CTG→CCG)  hybC ← hydrogenase 2 large subunit
RA 3,162,665 G→A S2L (TCA→TTA)  ftsP ← cell division protein required during stress conditions
RA 3,218,001 T→C N359S (AAC→AGC)  aer ← aerotaxis sensor receptor, flavoprotein
RA 3,230,481 A→G I532V (ATC→GTC)  ygjK → glycoside hydrolase
RA 3,236,458 A→G I167V (ATC→GTC)  ygjP → putative metal‑dependent hydrolase
RA 3,246,291 T→C R356R (CGT→CGC exuT → hexuronate transporter
RA 3,250,041 T→C L120S (TTA→TCA)  yqjE → conserved inner membrane protein YqjE
RA 3,253,216 T→C G102G (GGT→GGC yhaI → putative inner membrane protein
RA 3,254,126 T→C E30G (GAG→GGG)  yhaJ ← DNA‑binding transcriptional activator YhaJ
RA 3,267,471 A→G N31S (AAC→AGC)  tdcR → DNA‑binding transcriptional activator TdcR
RA 3,280,494 T→C V93A (GTG→GCG)  agaV → N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component
RA 3,283,780 T→C V213A (GTT→GCT)  kbaY → tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaY
RA 3,292,429 G→A R363R (CGG→CGA yraK → putative fimbrial adhesin YraK
RA 3,304,813 (C)7→8 coding (1005/1245 nt) mtr ← tryptophan:H(+) symporter Mtr
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,362,022 T→C intergenic (+82/‑90) gltF → / → yhcA periplasmic protein GltF/putative fimbrial chaperone YhcA
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,407,054 T→C Y380H (TAC→CAC)  accC → biotin carboxylase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,605,181 T→C V68A (GTA→GCA)  yhhL → DUF1145 domain‑containing protein YhhL
RA 3,619,269 A→G V26V (GTA→GTG rhsB → rhs element protein RhsB
RA 3,619,272 T→C R27R (CGT→CGC rhsB → rhs element protein RhsB
RA 3,627,563 A→T I659N (ATC→AAC)  rbbA ← ribosome‑associated ATPase
RA 3,668,544 (C)6→7 coding (645/1398 nt) ccp ← cytochrome c peroxidase
RA 3,728,170 T→C Y401C (TAC→TGC)  xylB ← xylulokinase
RA 3,744,307 A→G intergenic (+97/‑21) yiaL → / → yiaM DUF386 domain‑containing protein YiaL/2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
RA 3,772,322 T→C R14R (CGT→CGC mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,794,733 T→C A249A (GCT→GCC rfaD → ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,858,049 T→C D119G (GAC→GGC)  yidJ ← putative sulfatase/phosphatase
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,931,183 (G)7→8 intergenic (‑90/‑133) ravA ← / → kup regulatory ATPase RavA/K(+):H(+) symporter Kup
RA 3,980,280 A→G T109A (ACG→GCG)  rffM → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
RA 4,013,467 A→G T139A (ACC→GCC)  metE → cobalamin‑independent homocysteine transmethylase
RA 4,023,449 T→C G238G (GGT→GGC tatC → twin arginine protein translocation system ‑ TatC protein
RA 4,168,571 G→A intergenic (+124/‑70) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA
RA 4,200,155 (C)6→7 intergenic (+61/‑126) yjaG → / → hupA conserved protein YjaG/DNA‑binding protein HU‑alpha
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,393,067 T→C H334R (CAC→CGC)  queG ← epoxyqueuosine reductase
RA 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA 2'‑O‑ribose G2251 methyltransferase/conserved protein YjfI
RA 4,432,986 A→T I24N (ATC→AAC)  ytfF ← inner membrane protein YtfF
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,465,588 A→G V198A (GTT→GCT)  treB ← trehalose‑specific PTS enzyme IIBC component
RA 4,466,090 T→C T31A (ACT→GCT)  treB ← trehalose‑specific PTS enzyme IIBC component
RA 4,472,777 A→G intergenic (‑244/+37) pyrL ← / ← yjgH pyrBI operon leader peptide/RutC family protein YjgH
RA 4,490,708 C→G P144P (CCG→CCC idnR ← DNA‑binding transcriptional dual regulator IdnR
RA 4,519,416 C→T L303L (CTG→CTA yjhU ← KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU
RA 4,531,914 A→G intergenic (‑263/+523) sgcX ← / ← yjhP KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain/KpLE2 phage‑like element; putative methyltransferase YjhP
RA 4,548,170 A→G K143K (AAA→AAG fimF → type 1 fimbriae minor subunit FimF
RA 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,606,215 (G)8→7 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,606,330 (G)8→9 noncoding (72/87 nt) leuQ ← tRNA‑Leu
RA 4,623,776 G→A V114V (GTC→GTT lplA ← lipoate‑‑protein ligase A

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 225610–225723 225896–225737 15–287 31 [29] [27] 41 [rrlH] [rrlH]
* * ÷ NC_000913 226149–226299 228909–228689 2391–2761 32 [28] [29] 31 [rrlH]–rrfH [rrlH],rrfH
* * ÷ NC_000913 731807 732200–731992 186–394 34 [27] [28] 30 rhsC rhs element protein RhsC
* * ÷ NC_000913 732687–732809 734586–734476 1668–1900 30 [29] [29] 30 [rhsC]–[ybfO] [rhsC],ybfB,[ybfO]
* * ÷ NC_000913 734721–734826 734989–734945 120–269 31 [28] [29] 31 ybfO conserved protein, rhs‑like YbfO
* * ÷ NC_000913 1269479–1269543 1269664–1269555 13–186 30 [29] [29] 30 rdlA/ldrB putative antisense RNA regulator of LdrA/small toxic polypeptide LdrB
* * ÷ NC_000913 1270203–1270390 1270526–1270493 104–324 31 [26] [28] 34 ldrC–rdlC ldrC,rdlC
* * ÷ NC_000913 2726132–2729247 2730846–2729567 321–4715 30 [29] [29] 34 [rrfG]–[rrsG] [rrfG],rrlG,gltW,[rrsG]
* * ÷ NC_000913 3423733–3424234 3424526–3424238 5–794 30 [29] [26] 31 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)113 (1.190) 66/266 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3214947 =30 (0.310)35 (0.360) 26/270 2.4 54.2% intergenic (+59/+20) rpoD/mug RNA polymerase, sigma 70 (sigma D) factor/stationary phase mismatch/uracil DNA glycosylase
?NC_000913 4502090 = NA (NA)noncoding (1426/1426 nt) IS4 repeat region
* ? NC_000913 = 3214959NA (NA)63 (0.640) 36/274 1.4 NA intergenic (+71/+8) rpoD/mug RNA polymerase, sigma 70 (sigma D) factor/stationary phase mismatch/uracil DNA glycosylase
?NC_000913 = 4503515 NA (NA)noncoding (1/1426 nt) IS4 repeat region
* ? NC_000913 3363001 =134 (1.370)155 (1.600) 65/272 0.2 69.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)114 (1.170) 68/274 0.1 95.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA