breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 43,457 Δ1 bp 19.4% intergenic (+23/+70) PP_0040 → / ← cadA‑I hypothetical protein/cadmium translocating P‑type ATPase
RA 43,461:1 +A 19.4% intergenic (+27/+66) PP_0040 → / ← cadA‑I hypothetical protein/cadmium translocating P‑type ATPase
RA 67,746 C→T 13.3% P352S (CCG→TCG)  PP_0057 → major facilitator family transporter
RA 72,026 G→A 8.2% R275C (CGT→TGT)  glyQ ← glycine‑tRNA ligase subunit alpha
RA 76,882 G→C 14.1% A221G (GCC→GGC)  rsmB ← ribosomal RNA small subunit methyltransferase B
RA 115,951 T→G 11.8% L85W (TTG→TGG)  PP_0110 → CyoE‑like protoheme IX farnesyltransferase
RA 115,956 C→A 10.8% Q87K (CAA→AAA)  PP_0110 → CyoE‑like protoheme IX farnesyltransferase
RA 160,310 C→T 13.8% E27K (GAG→AAG)  PP_0149 ← hypothetical protein
RA 160,311 A→G 14.3% L26L (CTT→CTC PP_0149 ← hypothetical protein
RA 181,805 T→C 18.8% intergenic (+19/‑87) PP_r01 → / → PP_5SB 5S ribosomal RNA/5S ribosomal RNA
RA 181,811 C→T 19.4% intergenic (+25/‑81) PP_r01 → / → PP_5SB 5S ribosomal RNA/5S ribosomal RNA
RA 181,812 A→G 19.3% intergenic (+26/‑80) PP_r01 → / → PP_5SB 5S ribosomal RNA/5S ribosomal RNA
RA 181,818 G→A 18.2% intergenic (+32/‑74) PP_r01 → / → PP_5SB 5S ribosomal RNA/5S ribosomal RNA
RA 195,629 A→G 12.5% T379A (ACC→GCC)  PP_0168 → putative surface adhesion protein
RA 195,634 C→T 34.3% T380T (ACC→ACT PP_0168 → putative surface adhesion protein
RA 196,306 T→C 30.8% N604N (AAT→AAC PP_0168 → putative surface adhesion protein
RA 196,327 A→C 31.5% T611T (ACA→ACC PP_0168 → putative surface adhesion protein
RA 196,351 C→G 24.8% T619T (ACC→ACG PP_0168 → putative surface adhesion protein
RA 196,390 T→C 26.9% D632D (GAT→GAC PP_0168 → putative surface adhesion protein
RA 197,551 C→G 16.7% T1019T (ACC→ACG PP_0168 → putative surface adhesion protein
RA 197,572 G→A 27.3% K1026K (AAG→AAA PP_0168 → putative surface adhesion protein
RA 197,590 T→C 31.9% D1032D (GAT→GAC PP_0168 → putative surface adhesion protein
RA 289,046 G→C 14.3% G155A (GGC→GCC)  oprE → outer‑membrane porin E
RA 319,217 A→T 14.8% I176N (ATC→AAC)  PP_0264 ← sensor histidine kinase
RA 321,309 G→A 18.2% W234* (TGG→TAG)  aguA → agmatine deiminase
RA 322,135 G→A 22.2% intergenic (+420/+38) aguA → / ← PP_0267 agmatine deiminase/ferric siderophore receptor
RA 322,142 T→C 21.6% intergenic (+427/+31) aguA → / ← PP_0267 agmatine deiminase/ferric siderophore receptor
RA 474,195 Δ1 bp 11.8% coding (793/1983 nt) dnaG ← DNA primase
RA 474,199 G→C 12.3% I263M (ATC→ATG dnaG ← DNA primase
RA 474,202:1 +G 12.1% coding (786/1983 nt) dnaG ← DNA primase
RA 697,140 G→T 12.1% intergenic (+64/‑687) mmsA‑I → / → PP_16SD methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
RA 697,217 T→C 16.7% intergenic (+141/‑610) mmsA‑I → / → PP_16SD methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
RA 697,228 C→G 25.0% intergenic (+152/‑599) mmsA‑I → / → PP_16SD methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
RA 697,231 C→G 25.0% intergenic (+155/‑596) mmsA‑I → / → PP_16SD methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
RA 697,242 G→A 24.0% intergenic (+166/‑585) mmsA‑I → / → PP_16SD methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
RA 697,253 G→A 17.4% intergenic (+177/‑574) mmsA‑I → / → PP_16SD methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA
RA 699,725 A→G 53.0% intergenic (+115/‑129) PP_t06 → / → PP_23SD tRNA‑Ala/23S ribosomal RNA
RA 994,950 T→A 25.0% intergenic (+26/+99) PP_0859 → / ← PP_0860 ketoglutaramate omega‑amidase/sulfite reductase flavoprotein subunit
RA 994,951 T→A 24.9% intergenic (+27/+98) PP_0859 → / ← PP_0860 ketoglutaramate omega‑amidase/sulfite reductase flavoprotein subunit
RA 1,001,196 A→T 11.1% Q57L (CAG→CTG)  PP_0863 → hypothetical protein
RA 1,001,199 A→T 11.1% Q58L (CAG→CTG)  PP_0863 → hypothetical protein
RA 1,013,231 A→T 30.5% intergenic (+56/+75) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein
RA 1,013,237 C→A 27.3% intergenic (+62/+69) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein
RA 1,013,238 A→T 27.3% intergenic (+63/+68) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein
RA 1,013,244 G→C 31.6% intergenic (+69/+62) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein
RA 1,013,245 G→C 31.6% intergenic (+70/+61) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein
RA 1,013,251 A→T 32.6% intergenic (+76/+55) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein
RA 1,013,252 T→G 33.4% intergenic (+77/+54) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein
RA 1,013,258 A→T 35.4% intergenic (+83/+48) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein
RA 1,052,565 G→A 14.7% P269S (CCC→TCC)  PP_0910 ← hypothetical protein
RA 1,204,422 G→T 18.2% intergenic (+114/+46) gspN → / ← PP_1056 type II secretion pathway protein GspN/iron‑chelator utilization protein
RA 1,256,639 C→G 14.8% A325A (GCC→GCG mrp → ATP‑dependent Fe‑S cluster transferase
RA 1,327,127 C→T 58.6% intergenic (+97/‑196) PP_16SE → / → PP_23SE 16S ribosomal RNA/23S ribosomal RNA
RA 1,356,411 G→C 11.7% G94A (GGC→GCC)  PP_1181 → two‑component system response regulator
RA 1,403,596 G→A 23.6% intergenic (‑56/+25) PP_1227 ← / ← PP_1228 membrane protein/methyl‑accepting chemotaxis transducer
RA 1,403,597 T→C 23.5% intergenic (‑57/+24) PP_1227 ← / ← PP_1228 membrane protein/methyl‑accepting chemotaxis transducer
RA 1,463,031 A→T 17.4% intergenic (‑100/+42) algI ← / ← algL alginate O‑acetylase AlgI/alginate lyase
RA 1,463,032 A→T 17.4% intergenic (‑101/+41) algI ← / ← algL alginate O‑acetylase AlgI/alginate lyase
RA 1,469,815 A→G 14.3% V112A (GTG→GCG)  algK ← alginate biosynthesis protein AlgK
RA 1,469,818 A→T 16.6% L111Q (CTG→CAG)  algK ← alginate biosynthesis protein AlgK
RA 1,469,821 A→T 15.1% L110Q (CTG→CAG)  algK ← alginate biosynthesis protein AlgK
RA 1,469,824 C→T 16.2% R109H (CGC→CAC)  algK ← alginate biosynthesis protein AlgK
JC 1,648,886 Δ12 bp 53.7% intergenic (‑123/+162) oprB‑II ← / ← PP_1446 carbohydrate‑selective porin/TonB‑dependent receptor
RA 1,703,263 T→C 18.3% intergenic (+70/‑97) lysS → / → PP_1497 lysine‑‑tRNA ligase/TetR family transcriptional regulator
RA 1,728,698 A→G 20.9% intergenic (+50/+73) cspA‑I → / ← PP_1523 major cold shock protein/hypothetical protein
RA 1,728,699 C→T 20.1% intergenic (+51/+72) cspA‑I → / ← PP_1523 major cold shock protein/hypothetical protein
RA 1,745,342 T→C 18.1% E154G (GAG→GGG)  PP_1543 ← hypothetical protein
RA 1,851,762 C→T 43.7% S269L (TCG→TTG)  relA → ATP:GTP 3'‑pyrophosphotransferase
RA 1,852,053 G→T 37.5% W366L (TGG→TTG)  relA → ATP:GTP 3'‑pyrophosphotransferase
RA 1,903,222 A→G 23.6% Q1244R (CAG→CGG)  PP_1703 → assimilatory nitrate reductase/sulfite reductase
RA 2,072,728 C→T 19.7% L241L (CTG→CTA PP_1847 ← TonB‑dependent ferric siderophore receptor
RA 2,100,098 G→C 14.8% intergenic (‑166/‑39) PP_1875 ← / → PP_1876 two‑component system sensor histidine kinase/response regulator/AAA family ATP/GTP‑binding protein
RA 2,100,099 C→A 14.8% intergenic (‑167/‑38) PP_1875 ← / → PP_1876 two‑component system sensor histidine kinase/response regulator/AAA family ATP/GTP‑binding protein
RA 2,112,775 C→A 18.2% P518P (CCG→CCT) ‡ PP_1882 ← membrane protein
RA 2,112,776 G→C 18.2% P518R (CCG→CGG) ‡ PP_1882 ← membrane protein
RA 2,112,781 G→C 19.1% H516Q (CAC→CAG) ‡ PP_1882 ← membrane protein
RA 2,112,782 T→G 19.1% H516P (CAC→CCC) ‡ PP_1882 ← membrane protein
RA 2,282,813 T→G 46.7% intergenic (‑162/+67) PP_2010 ← / ← PP_2011 cytochrome b561/hypothetical protein
RA 2,282,822 C→A 43.9% intergenic (‑171/+58) PP_2010 ← / ← PP_2011 cytochrome b561/hypothetical protein
RA 2,478,883 G→A 23.5% intergenic (+93/+352) PP_2172 → / ← PP_2173 hypothetical protein/AraC family transcriptional regulator
RA 2,478,884 C→A 23.5% intergenic (+94/+351) PP_2172 → / ← PP_2173 hypothetical protein/AraC family transcriptional regulator
RA 2,478,888 G→C 23.5% intergenic (+98/+347) PP_2172 → / ← PP_2173 hypothetical protein/AraC family transcriptional regulator
RA 2,478,892 T→G 23.5% intergenic (+102/+343) PP_2172 → / ← PP_2173 hypothetical protein/AraC family transcriptional regulator
RA 2,478,893 T→C 23.5% intergenic (+103/+342) PP_2172 → / ← PP_2173 hypothetical protein/AraC family transcriptional regulator
RA 2,514,934 G→C 25.0% P166A (CCG→GCG)  phnX ← phosphonoacetaldehyde hydrolase
RA 2,531,947 T→A 21.0% A53A (GCT→GCA PP_2222 → hypothetical protein
RA 2,546,866 T→C 40.3% intergenic (+22/+18) PP_2238 → / ← PP_5504 metallopeptidase/hypothetical protein
RA 2,546,869 G→A 40.2% intergenic (+25/+15) PP_2238 → / ← PP_5504 metallopeptidase/hypothetical protein
RA 2,673,220 A→C 22.2% D164A (GAT→GCT)  PP_2340 → hypothetical protein
RA 2,673,222:1 +C 22.2% coding (493/519 nt) PP_2340 → hypothetical protein
RA 2,673,232 G→T 22.2% S168I (AGC→ATC)  PP_2340 → hypothetical protein
RA 2,717,206 G→C 15.4% A626A (GCG→GCC cti → esterified fatty acid cis/trans isomerase
RA 2,800,111 C→A 26.8% E75* (GAA→TAA)  ansB ← glutaminase‑asparaginase
RA 2,824,560 G→T 40.0% E44* (GAG→TAG) ‡ PP_2478 → isoquinoline 1‑oxidoreductase subunit beta
RA 2,824,561 A→C 36.6% E44A (GAG→GCG) ‡ PP_2478 → isoquinoline 1‑oxidoreductase subunit beta
RA 2,951,377 G→C 50.0% intergenic (‑113/+48) oguA ← / ← PP_2585 8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
RA 2,951,384 A→C 40.0% intergenic (‑120/+41) oguA ← / ← PP_2585 8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
RA 2,951,385 G→T 39.5% intergenic (‑121/+40) oguA ← / ← PP_2585 8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
RA 2,951,392 G→C 28.6% intergenic (‑128/+33) oguA ← / ← PP_2585 8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
RA 3,018,022 C→A 24.9% R634R (CGC→CGA bcsB → cellulose synthase and translocator subunit
RA 3,350,711 G→A 16.0% G389S (GGC→AGC)  PP_2945 → two‑component system sensor histidine kinase/response regulator
RA 3,628,125 C→G 23.9% intergenic (+11/‑129) PP_3198 → / → PP_3199 2Fe‑2S ferredoxin/monooxygenase
RA 3,630,695 T→A 26.7% W425R (TGG→AGG)  PP_3200 → hypothetical protein
RA 3,630,700 T→G 28.6% L426L (CTT→CTG PP_3200 → hypothetical protein
RA 3,630,703 C→A 28.1% A427A (GCC→GCA PP_3200 → hypothetical protein
RA 3,630,708 T→A 26.7% L429Q (CTG→CAG)  PP_3200 → hypothetical protein
RA 3,670,152 C→T 19.1% R135C (CGT→TGT)  fnrB → Crp/Fnr family transcriptional regulator
RA 3,816,546 A→T 27.6% G106G (GGT→GGA cpxR ← two‑component system regulator CpxR
RA 4,002,726 A→G 23.3% intergenic (+29/‑61) allE → / → PP_3531 S‑ureidoglycine aminohydrolase/hypothetical protein
RA 4,002,727 C→G 20.7% intergenic (+30/‑60) allE → / → PP_3531 S‑ureidoglycine aminohydrolase/hypothetical protein
RA 4,002,728 C→T 20.6% intergenic (+31/‑59) allE → / → PP_3531 S‑ureidoglycine aminohydrolase/hypothetical protein
RA 4,071,255 A→G 21.0% D316D (GAT→GAC mdtB ← multidrug transporter membrane protein
RA 4,094,305 A→G 22.2% T420A (ACC→GCC)  PP_3602 → 2,5‑dioxovalerate dehydrogenase
RA 4,110,826 A→G 26.7% L868L (CTA→CTG PP_3616 → membrane protein
RA 4,117,163 G→A 12.6% W88* (TGG→TGA iorA‑II → isoquinoline 1‑oxidoreductase subunit alpha
RA 4,162,143 T→C 17.8% intergenic (‑43/+34) PP_3663 ← / ← pssA GGDEF domain‑containing protein/CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
RA 4,164,734 A→T 16.7% intergenic (+40/+77) PP_3665 → / ← PP_3666 AraC family transcriptional regulator/MHS family metabolite MFS transporter
RA 4,164,735 A→T 16.7% intergenic (+41/+76) PP_3665 → / ← PP_3666 AraC family transcriptional regulator/MHS family metabolite MFS transporter
RA 4,171,851 T→A 13.8% T106S (ACG→TCG)  PP_3670 ← DMT superfamily permease
RA 4,171,856 T→A 14.3% E104V (GAG→GTG)  PP_3670 ← DMT superfamily permease
RA 4,348,749 A→G 41.2% intergenic (‑70/+404) PP_3820 ← / ← galU group II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
RA 4,399,566 A→T 30.9% I123N (ATT→AAT)  PP_3878 ← minor capsid protein C
RA 4,399,571 T→C 30.8% E121E (GAA→GAG PP_3878 ← minor capsid protein C
RA 4,501,014 C→A 14.8% G31V (GGG→GTG)  PP_3989 ← DNA‑cytosine methyltransferase
RA 4,520,498 A→T 21.1% intergenic (+45/+27) cspD → / ← icd DNA replication inhibitor/NADP(+)‑specific isocitrate dehydrogenase
RA 4,520,508 A→G 21.1% intergenic (+55/+17) cspD → / ← icd DNA replication inhibitor/NADP(+)‑specific isocitrate dehydrogenase
JC 4,712,487 Δ33 bp 38.7% coding (673‑705/2115 nt) PP_4171 → hypothetical protein
RA 4,740,884 C→G 17.4% intergenic (+75/+677) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,273:1 +C 66.7% intergenic (+464/+288) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,274 A→C 33.3% intergenic (+465/+287) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,275 A→G 60.4% intergenic (+466/+286) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,277 C→T 100% intergenic (+468/+284) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,280 C→T 100% intergenic (+471/+281) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,283 C→G 100% intergenic (+474/+278) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,285 2 bp→CT 100% intergenic (+476/+275) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,759,592 C→T 18.2% T79T (ACG→ACA pvdP ← pyoverdine biosynthesis‑like protein
RA 4,783,733 A→G 12.1% L1209P (CTG→CCG)  pvdJ ← non‑ribosomal peptide synthetase
RA 4,783,736 C→T 12.1% R1208Q (CGG→CAG)  pvdJ ← non‑ribosomal peptide synthetase
RA 5,130,031 A→T 13.8% L474Q (CTG→CAG)  recQ ← ATP‑dependent DNA 3'‑5' helicase
RA 5,173,292 T→C 15.4% intergenic (‑285/+47) fadD‑I ← / ← fadD‑II long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase
RA 5,173,295 G→C 14.8% intergenic (‑288/+44) fadD‑I ← / ← fadD‑II long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase
RA 5,173,296 G→C 14.8% intergenic (‑289/+43) fadD‑I ← / ← fadD‑II long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase
RA 5,173,299 G→A 12.5% intergenic (‑292/+40) fadD‑I ← / ← fadD‑II long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase
RA 5,183,577 T→C 14.8% K69E (AAG→GAG)  PP_4563 ← hypothetical protein
RA 5,194,935 T→G 19.1% intergenic (+29/+24) PP_4574 → / ← PP_4575 hypothetical protein/urea amidolyase‑like protein
RA 5,194,936 C→A 18.3% intergenic (+30/+23) PP_4574 → / ← PP_4575 hypothetical protein/urea amidolyase‑like protein
RA 5,212,535 A→G 20.0% intergenic (+51/+43) PP_4593 → / ← PP_4594 hypothetical protein/cystathionine gamma‑synthase
RA 5,212,539 T→G 20.0% intergenic (+55/+39) PP_4593 → / ← PP_4594 hypothetical protein/cystathionine gamma‑synthase
RA 5,212,540 C→T 21.1% intergenic (+56/+38) PP_4593 → / ← PP_4594 hypothetical protein/cystathionine gamma‑synthase
RA 5,268,815 A→G 10.2% A451A (GCA→GCG PP_4643 → xanthine/uracil permease family protein
RA 5,269,306 C→A 10.0% G361C (GGC→TGC)  radA ← DNA repair enzyme
RA 5,381,315 T→C 18.8% intergenic (+32/+33) bamE → / ← PP_4732 outer membrane protein assembly factor BamE/hypothetical protein
RA 5,381,316 T→A 18.8% intergenic (+33/+32) bamE → / ← PP_4732 outer membrane protein assembly factor BamE/hypothetical protein
RA 5,381,317 G→A 18.8% intergenic (+34/+31) bamE → / ← PP_4732 outer membrane protein assembly factor BamE/hypothetical protein
RA 5,450,969 C→A 15.2% I100I (ATC→ATA corC → Co(2+)/Mg(2+)efflux heat shock protein
RA 5,559,994 T→A 11.1% Q185L (CAG→CTG)  hisZ ← ATP phosphoribosyltransferase regulatory subunit
RA 5,569,322 G→A 15.4% L44L (CTG→TTG)  tsaE ← ATPase
RA 5,653,045 C→A 13.8% intergenic (‑95/+66) PP_4961 ← / ← PP_4962 lipoprotein/hypothetical protein
JC 5,657,310 +GACGAT 50.6% coding (436/1998 nt) tktA ← transketolase A
RA 5,675,706 G→A 13.8% A96V (GCC→GTC)  yceJ ← iron‑regulated cytochrome b561
RA 5,725,708 C→G 9.7% P438P (CCG→CCC opgH ← glucans biosynthesis glucosyltransferase H
RA 5,790,193 G→A 14.1% intergenic (+16/+52) ynfM → / ← PP_5073 efflux transporter/hypothetical protein
RA 5,808,040 A→G 15.9% intergenic (+119/+36) mrcA → / ← maeB penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B
RA 5,808,041 T→C 20.3% intergenic (+120/+35) mrcA → / ← maeB penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B
RA 5,808,042 G→A 17.5% intergenic (+121/+34) mrcA → / ← maeB penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B
RA 5,808,043 C→T 16.8% intergenic (+122/+33) mrcA → / ← maeB penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B
RA 5,815,587 C→T 12.8% intergenic (+15/+76) PP_5090 → / ← PP_5091 cell division protein/membrane protein
RA 5,815,592 G→T 10.0% intergenic (+20/+71) PP_5090 → / ← PP_5091 cell division protein/membrane protein
RA 5,815,595 A→C 11.9% intergenic (+23/+68) PP_5090 → / ← PP_5091 cell division protein/membrane protein
RA 5,815,600 A→G 13.1% intergenic (+28/+63) PP_5090 → / ← PP_5091 cell division protein/membrane protein
RA 5,897,462 T→C 24.5% intergenic (‑18/+12) sbp‑II ← / ← PP_5172 sulfate ABC transporter substrate‑binding protein/hypothetical protein
RA 5,903,220 G→T 17.8% intergenic (‑128/‑140) PP_5175 ← / → PP_5176 HlyD family secretion protein/hypothetical protein
RA 5,903,221 A→C 17.9% intergenic (‑129/‑139) PP_5175 ← / → PP_5176 HlyD family secretion protein/hypothetical protein
RA 5,988,961 T→C 34.8% intergenic (+199/‑347) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,988,967 Δ1 bp 34.8% intergenic (+205/‑341) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
RA 5,997,245:1 +G 21.7% intergenic (‑158/+42) PP_5252 ← / ← PP_5253 hypothetical protein/arylesterase
RA 5,997,250 Δ1 bp 21.7% intergenic (‑163/+37) PP_5252 ← / ← PP_5253 hypothetical protein/arylesterase
RA 6,078,789 G→T 14.8% L165M (CTG→ATG)  PP_5330 ← MFS transporter
RA 6,172,551 G→T 12.9% A196E (GCG→GAG)  PP_5410 ← DeoR family transcriptional regulator

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947 171396–172964 176266–172972 9–4871 2 [1] [0] 3 [PP_16SA]–[PP_5SA] [PP_16SA],PP_23SA,[PP_5SA]
* * ÷ NC_002947 176688–178394 181686–178402 9–4999 2 [1] [1] 2 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947 2197191 2197209 19 3 [1] [1] 2 PP_1942 LysR family transcriptional regulator
* * ÷ NC_002947 2619372 2619395 24 2 [1] [0] 2 PP_2292/PP_2293 hypothetical protein/DNA maturase B
* * ÷ NC_002947 2812584 2812594 11 3 [1] [0] 2 rplT 50S ribosomal protein L20
* * ÷ NC_002947 2858627 2858695 69 2 [1] [1] 2 [PP_2510] [PP_2510]
* * ÷ NC_002947 2991627 2991701 75 2 [1] [1] 2 hcpC‑I hemolysin‑coregulated protein
* * ÷ NC_002947 4808211 4808218 8 3 [1] [0] 8 PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 = 42614430 (1.550)4 (0.220) 4/246 NT 11.7% coding (1658/2433 nt) PP_0350 ferrichrome‑iron receptor
?NC_002947 = 426167 32 (1.740)coding (1635/2433 nt) PP_0350 ferrichrome‑iron receptor
* ? NC_002947 = 44508819 (0.980)5 (0.330) 4/204 NT 20.4% intergenic (+58/‑230) PP_0367/PP_0368 hypothetical protein/acyl‑CoA dehydrogenase
?NC_002947 = 445127 24 (1.570)intergenic (+97/‑191) PP_0367/PP_0368 hypothetical protein/acyl‑CoA dehydrogenase
* ? NC_002947 = 742614NA (NA)7 (0.380) 6/248 NT NA coding (712/1422 nt) PP_0635 group II intron‑encoding maturase
?NC_002947 = 1426852 NA (NA)coding (743/1422 nt) PP_1250 group II intron‑encoding maturase
* ? NC_002947 = 78660536 (1.860)3 (0.170) 3/236 NT 9.9% intergenic (+24/‑281) PP_0673/ettA hypothetical protein/ADP/ATP ratio sensor/translation inhibitor
?NC_002947 = 786691 22 (1.250)intergenic (+110/‑195) PP_0673/ettA hypothetical protein/ADP/ATP ratio sensor/translation inhibitor
* ? NC_002947 836205 =22 (1.140)9 (0.510) 6/236 NT 35.9% intergenic (‑124/+53) PP_0718/ychF sulfate transporter/ribosome‑associated potassium‑dependent ATP/GTPase
?NC_002947 836237 = 12 (0.680)intergenic (‑156/+21) PP_0718/ychF sulfate transporter/ribosome‑associated potassium‑dependent ATP/GTPase
* ? NC_002947 867938 =21 (1.090)5 (0.290) 5/228 NT 20.6% intergenic (+33/+144) PP_0750/mqo‑I hypothetical protein/malate:quinone oxidoreductase
?NC_002947 867998 = 20 (1.170)intergenic (+93/+84) PP_0750/mqo‑I hypothetical protein/malate:quinone oxidoreductase
* ? NC_002947 1642089 =10 (0.520)3 (0.160) 3/250 NT 20.9% coding (126/393 nt) PP_1442 hypothetical protein
?NC_002947 1642134 = 13 (0.690)coding (81/393 nt) PP_1442 hypothetical protein
* ? NC_002947 = 17462988 (0.410)3 (0.160) 3/248 NT 23.4% coding (210/387 nt) PP_1544 hypothetical protein
?NC_002947 = 1746325 12 (0.650)coding (183/387 nt) PP_1544 hypothetical protein
* ? NC_002947 = 181147924 (1.240)3 (0.190) 3/212 NT 12.9% intergenic (+44/+84) ispD/PP_1615 2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase/LysR family transcriptional regulator
?NC_002947 = 1811513 21 (1.320)intergenic (+78/+50) ispD/PP_1615 2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase/LysR family transcriptional regulator
* ? NC_002947 2047296 =11 (0.570)4 (0.220) 4/248 NT 27.1% coding (768/1197 nt) PP_1820 benzoate transport protein
?NC_002947 2047345 = 11 (0.590)coding (719/1197 nt) PP_1820 benzoate transport protein
* ? NC_002947 = 205063811 (0.570)4 (0.290) 4/184 NT 32.2% intergenic (‑88/+87) PP_1824/PP_1825 Smr domain‑containing protein/hypothetical protein
?NC_002947 = 2050674 9 (0.660)intergenic (‑124/+51) PP_1824/PP_1825 Smr domain‑containing protein/hypothetical protein
* ? NC_002947 2100003 =10 (0.520)8 (0.500) 4/212 NT 45.5% intergenic (‑71/‑134) PP_1875/PP_1876 two‑component system sensor histidine kinase/response regulator/AAA family ATP/GTP‑binding protein
?NC_002947 2100098 = 11 (0.690)intergenic (‑166/‑39) PP_1875/PP_1876 two‑component system sensor histidine kinase/response regulator/AAA family ATP/GTP‑binding protein
* ? NC_002947 2112769 =16 (0.830)4 (0.220) 4/246 NT 20.4% coding (1560/4899 nt) PP_1882 membrane protein
?NC_002947 2112789 = 16 (0.870)coding (1540/4899 nt) PP_1882 membrane protein
* ? NC_002947 2295818 =11 (0.570)3 (0.190) 3/210 NT 26.2% intergenic (+21/+112) PP_5496/PP_2023 hypothetical protein/glutathione S‑transferase family protein
?NC_002947 2295903 = 8 (0.510)intergenic (+106/+27) PP_5496/PP_2023 hypothetical protein/glutathione S‑transferase family protein
* ? NC_002947 2301539 =17 (0.880)3 (0.170) 3/230 NT 15.7% intergenic (‑140/‑191) sbcD/PP_5497 exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein
?NC_002947 2301624 = 17 (0.990)intergenic (‑225/‑106) sbcD/PP_5497 exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein
* ? NC_002947 2310567 =10 (0.520)3 (0.170) 3/238 NT 22.0% coding (275/1311 nt) PP_2033 hypothetical protein
?NC_002947 2310624 = 12 (0.670)coding (332/1311 nt) PP_2033 hypothetical protein
* ? NC_002947 = 279496515 (0.780)6 (0.350) 6/232 NT 28.9% intergenic (+30/+82) PP_2447/puuB hypothetical protein/gamma‑glutamylputrescine oxidase
?NC_002947 = 2795016 16 (0.920)intergenic (+81/+31) PP_2447/puuB hypothetical protein/gamma‑glutamylputrescine oxidase
* ? NC_002947 3387807 =10 (0.520)3 (0.170) 3/242 NT 23.6% coding (545/795 nt) PP_2989 short‑chain dehydrogenase/reductase family oxidoreductase
?NC_002947 3387849 = 10 (0.550)coding (587/795 nt) PP_2989 short‑chain dehydrogenase/reductase family oxidoreductase
* ? NC_002947 3483915 =11 (0.570)5 (0.270) 5/250 NT 37.5% coding (2052/3804 nt) PP_3091 membrane protein
?NC_002947 3483952 = 6 (0.320)coding (2015/3804 nt) PP_3091 membrane protein
* ? NC_002947 = 360798718 (0.930)4 (0.220) 4/244 NT 17.0% coding (1173/2265 nt) PP_3182 GGDEF domain‑containing protein
?NC_002947 = 3608006 22 (1.200)coding (1154/2265 nt) PP_3182 GGDEF domain‑containing protein
* ? NC_002947 = 404209024 (1.240)3 (0.160) 3/250 NT 12.4% coding (134/852 nt) PP_3563 hypothetical protein
?NC_002947 = 4042115 19 (1.010)coding (159/852 nt) PP_3563 hypothetical protein
* ? NC_002947 = 45861003 (0.160)5 (0.370) 5/182 NT 55.2% intergenic (+139/+78) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
?NC_002947 = 4586128 6 (0.440)intergenic (+167/+50) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
* ? NC_002947 = 458804324 (1.240)4 (0.230) 4/230 NT 14.4% intergenic (‑183/‑139) PP_4063/ivd long‑chain fatty acid‑‑CoA ligase/isovaleryl‑CoA dehydrogenase
?NC_002947 = 4588104 26 (1.510)intergenic (‑244/‑78) PP_4063/ivd long‑chain fatty acid‑‑CoA ligase/isovaleryl‑CoA dehydrogenase
* ? NC_002947 4808219 =0 (0.000)6 (0.430) 4/188 NT 57.1% intergenic (‑283/‑272) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947 = 5988991 9 (0.640)intergenic (+229/‑317) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947 = 488620915 (0.780)7 (0.470) 6/200 NT 32.9% intergenic (+43/+80) PP_4295/PP_4296 TetR family transcriptional regulator/hypothetical protein
?NC_002947 = 4886238 17 (1.140)intergenic (+72/+51) PP_4295/PP_4296 TetR family transcriptional regulator/hypothetical protein
* ? NC_002947 5162618 =27 (1.400)5 (0.320) 5/206 NT 26.0% intergenic (+93/+108) PP_4544/PP_4545 hypothetical protein/beta‑ketodecanoyl‑ACP synthase
?NC_002947 5162702 = 7 (0.450)intergenic (+177/+24) PP_4544/PP_4545 hypothetical protein/beta‑ketodecanoyl‑ACP synthase
* ? NC_002947 = 520522120 (1.030)5 (0.270) 5/250 NT 23.1% intergenic (+64/+51) PP_4585/PP_4586 TrmH family RNA methyltransferase/hypothetical protein
?NC_002947 = 5205267 14 (0.750)intergenic (+110/+5) PP_4585/PP_4586 TrmH family RNA methyltransferase/hypothetical protein
* ? NC_002947 = 567168922 (1.140)4 (0.250) 4/210 NT 18.7% intergenic (+57/‑113) PP_4978/PP_4979 hypothetical protein/substrate‑binding protein
?NC_002947 = 5671726 17 (1.080)intergenic (+94/‑76) PP_4978/PP_4979 hypothetical protein/substrate‑binding protein
* ? NC_002947 = 569261820 (1.030)8 (0.450) 5/236 NT 27.0% intergenic (+31/‑139) PP_4994/PP_4995 hypothetical protein/exopolysaccharide biosynthesis protein
?NC_002947 = 5692680 25 (1.420)intergenic (+93/‑77) PP_4994/PP_4995 hypothetical protein/exopolysaccharide biosynthesis protein
* ? NC_002947 = 573409721 (1.090)4 (0.230) 4/234 NT 17.0% intergenic (‑67/+90) proY/hutH proline (histidine) APC transporter/histidine ammonia‑lyase
?NC_002947 = 5734152 20 (1.140)intergenic (‑122/+35) proY/hutH proline (histidine) APC transporter/histidine ammonia‑lyase
* ? NC_002947 = 58169278 (0.410)5 (0.300) 4/222 NT 40.2% intergenic (+57/+72) PP_5092/pilT NLP/P60 protein/twitching motility protein
?NC_002947 = 5816954 8 (0.480)intergenic (+84/+45) PP_5092/pilT NLP/P60 protein/twitching motility protein
* ? NC_002947 = 587706331 (1.600)4 (0.250) 4/210 NT 12.3% intergenic (+29/+76) rpiA/PP_5151 ribose‑5‑phosphate isomerase A/hypothetical protein
?NC_002947 = 5877097 32 (2.040)intergenic (+63/+42) rpiA/PP_5151 ribose‑5‑phosphate isomerase A/hypothetical protein
* ? NC_002947 6000957 =30 (1.550)6 (0.310) 6/256 NT 16.5% intergenic (+8/+101) PP_5256/amaA cyclic nucleotide‑binding protein/L‑pipecolate oxidase
?NC_002947 6001055 = 31 (1.620)intergenic (+106/+3) PP_5256/amaA cyclic nucleotide‑binding protein/L‑pipecolate oxidase
* ? NC_002947 = 602269019 (0.980)4 (0.220) 4/246 NT 16.3% coding (842/1293 nt) PP_5273 oxidoreductase
?NC_002947 = 6022719 23 (1.250)coding (871/1293 nt) PP_5273 oxidoreductase
* ? NC_002947 = 608022324 (1.240)4 (0.220) 4/246 NT 14.6% coding (111/897 nt) PP_5332 hypothetical protein
?NC_002947 = 6080255 24 (1.300)coding (143/897 nt) PP_5332 hypothetical protein