breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 7,677 T→C 11.0% M95V (ATG→GTG)  Y75_RS00040 ← sodium:alanine symporter family protein
RA 7,710 T→A 10.6% I84F (ATT→TTT)  Y75_RS00040 ← sodium:alanine symporter family protein
RA 82,766 A→G 12.0% L255P (CTC→CCC)  leuA ← 2‑isopropylmalate synthase
RA 228,688 Δ1 bp 100% intergenic (+9/‑70) Y75_RS01035 → / → rrf 23S ribosomal RNA/5S ribosomal RNA
RA 249,538 T→G 35.1% H187P (CAC→CCC)  flhA ← flagellar type III secretion system protein FlhA
RA 249,540 C→G 14.0% P186P (CCG→CCC flhA ← flagellar type III secretion system protein FlhA
RA 260,444 T→A 9.1% I278N (ATC→AAC)  proB → glutamate 5‑kinase
RA 284,748 A→G 13.0% I44V (ATT→GTT)  Y75_RS01395 → MFS transporter
RA 296,909 A→C 7.6% V36G (GTT→GGT)  Y75_RS01460 ← hypothetical protein
RA 297,575 A→G 11.1% S126P (TCG→CCG)  paoD ← molybdenum cofactor insertion chaperone PaoD
RA 303,229 C→A 10.3% A60S (GCC→TCC)  Y75_RS01490 ← YkgJ family cysteine cluster protein
RA 304,460 A→C 10.1% F528V (TTT→GTT)  ecpD ← fimbrial adhesin EcpD
RA 339,916 A→C 8.3% A176A (GCA→GCC Y75_RS01670 → carbamate kinase family protein
RA 346,895 G→C 11.4% A258G (GCG→GGG)  prpR ← propionate catabolism operon regulatory protein PrpR
RA 359,586 T→G 14.3% V133G (GTG→GGG)  cynX → cyanate transporter CynX
RA 368,917 T→C 12.5% A361A (GCT→GCC Y75_RS01785 → bifunctional 3‑(3‑hydroxy‑phenyl)propionate/3‑hydroxycinnamic acid hydroxylase
RA 385,021 T→G 12.3% V189G (GTG→GGG)  tauA → taurine ABC transporter substrate‑binding protein
RA 386,930 A→G 9.0% I246V (ATC→GTC)  tauC → taurine ABC transporter permease TauC
RA 396,785 A→C 6.3% N308T (AAC→ACC)  sbmA → peptide antibiotic transporter SbmA
RA 416,826 A→G 8.9% E154G (GAG→GGG)  phoB → phosphate response regulator transcription factor PhoB
RA 421,445 G→T 7.9% L412L (CTG→CTT proY → proline‑specific permease ProY
RA 421,455 A→T 9.8% I416F (ATC→TTC)  proY → proline‑specific permease ProY
RA 432,873 T→G 28.4% G65G (GGT→GGG ribD → bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase RibD
RA 471,229 A→G 10.8% E457G (GAG→GGG)  Y75_RS02310 → SmdB family multidrug efflux ABC transporter permease/ATP‑binding protein
RA 487,626 A→C 8.6% T623P (ACC→CCC)  mscK → mechanosensitive channel MscK
RA 547,694 A→G 100% E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,831:1 +G 100% coding (251/261 nt) Y75_RS02655 → hypothetical protein
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
RA 564,474 G→T 11.7% S243* (TCA→TAA)  Y75_RS02750 ← site‑specific integrase
RA 594,075 G→C 11.4% L198V (CTG→GTG)  cusR ← copper response regulator transcription factor CusR
RA 622,087 A→T 7.1% T189S (ACC→TCC)  entS → enterobactin transporter EntS
RA 629,465 A→G 8.3% M117V (ATG→GTG)  cstA → carbon starvation protein CstA
MC JC 654,214 Δ1,199 bp 100% Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
RA 659,182 T→G 8.4% pseudogene (730/788 nt) Y75_RS03250 → deaminated glutathione amidase
RA 693,319 T→C 7.9% E174G (GAG→GGG)  Y75_RS03425 ← PhoH family protein
RA 747,683 T→G 10.7% N170T (AAC→ACC)  Y75_RS03700 ← AbrB family transcriptional regulator
RA 795,452 A→G 89.4% intergenic (+108/‑59) Y75_RS03980 → / → modA multidrug efflux pump accessory protein AcrZ/molybdate ABC transporter substrate‑binding protein
RA 812,143 T→G 29.8% V67G (GTG→GGG)  bioC → malonyl‑ACP O‑methyltransferase BioC
RA 822,425 T→A 7.3% I166F (ATT→TTT)  Y75_RS04110 ← UPF0104 family protein
RA 888,454 T→A 10.8% intergenic (+73/+102) Y75_RS04405 → / ← Y75_RS04410 DNA‑binding transcriptional regulator RcdA/aspartate:alanine antiporter
RA 974,882 A→G 8.8% S48G (AGT→GGT)  mukF → chromosome partition protein MukF
RA 981,675 T→G 14.0% G69G (GGT→GGG ldtD → L,D‑transpeptidase
RA 990,367 A→G 14.6% S138P (TCA→CCA)  pncB ← nicotinate phosphoribosyltransferase
RA 1,058,908 T→C 9.8% S135P (TCG→CCG)  torC → pentaheme c‑type cytochrome TorC
RA 1,065,700 C→T 100% R415C (CGT→TGT)  Y75_RS05220 → YccE family protein
RA 1,077,331 A→C 14.3% G658G (GGT→GGG putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA 1,081,122 A→G 10.9% I466V (ATT→GTT)  putP → sodium/proline symporter PutP
RA 1,093,686 T→C 100% V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
RA 1,158,866 A→G 8.9% G171G (GGA→GGG Y75_RS05755 → metal‑dependent hydrolase
RA 1,239,439 A→C 11.0% T98P (ACC→CCC)  Y75_RS06220 → D‑amino acid dehydrogenase
RA 1,262,461 C→A 8.9% intergenic (‑81/+44) dauA ← / ← Y75_RS06315 C4‑dicarboxylic acid transporter DauA/ribose‑phosphate diphosphokinase
RA 1,273,414 G→T 7.3% Q5K (CAA→AAA)  chaA ← sodium‑potassium/proton antiporter ChaA
RA 1,303,712 A→G 100% S273G (AGC→GGC)  oppA → oligopeptide ABC transporter substrate‑binding protein OppA
RA 1,306,094 G→T 9.6% V183L (GTG→TTG)  oppC → oligopeptide ABC transporter permease OppC
RA 1,324,175 C→G 10.1% P162P (CCG→CCC trpE ← anthranilate synthase component I
RA 1,327,725 A→G 11.3% G422G (GGA→GGG Y75_RS06660 → DUF2207 domain‑containing protein
RA 1,379,311 G→T 8.4% A256S (GCA→TCA)  Y75_RS06935 → Gfo/Idh/MocA family oxidoreductase
RA 1,385,434 G→A 12.4% S81F (TCT→TTT)  Y75_RS06960 ← LacI family transcriptional regulator
MC JC 1,413,639 Δ23,060 bp 100% [ttcA]Y75_RS24865 33 genes
RA 1,441,997 C→A 7.6% D168Y (GAT→TAT)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,447,718 T→G 13.3% V42G (GTT→GGT)  Y75_RS07310 → YdbL family protein
RA 1,467,915 T→C 7.6% pseudogene (810/2526 nt) Y75_RS07400 → autotransporter domain‑containing protein
RA 1,468,137 T→C 5.4% pseudogene (1032/2526 nt) Y75_RS07400 → autotransporter domain‑containing protein
RA 1,478,899 T→C 8.5% L681P (CTC→CCC)  Y75_RS07430 → DUF2773 domain‑containing bactofilin
RA 1,521,793 C→G 11.1% intergenic (+15/‑183) curA → / → Y75_RS07665 NADPH‑dependent curcumin/dihydrocurcumin reductase/DNA‑binding transcriptional regulator McbR
RA 1,521,798 G→A 11.7% intergenic (+20/‑178) curA → / → Y75_RS07665 NADPH‑dependent curcumin/dihydrocurcumin reductase/DNA‑binding transcriptional regulator McbR
RA 1,552,363 C→G 12.5% V63V (GTC→GTG fdxH → formate dehydrogenase N subunit beta
RA 1,703,339 A→G 14.5% G223G (GGA→GGG malY → bifunctional maltose regulon transcriptional repressor/cystathionine beta‑lyase MalY
RA 1,739,298 A→G 100% intergenic (‑39/‑260) ynhF ← / → purR stress response protein YnhF/HTH‑type transcriptional repressor PurR
RA 1,767,050 G→C 8.9% R99R (CGC→CGG menI ← 1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
RA 1,826,878 A→T 6.4% G154G (GGT→GGA spy ← ATP‑independent periplasmic protein‑refolding chaperone
RA 1,898,302 T→C 13.2% L140P (CTC→CCC)  Y75_RS09545 → CoA pyrophosphatase
RA 1,961,858 T→G 14.5% V28G (GTT→GGT)  argS → arginine‑‑tRNA ligase
RA 1,971,688 A→C 16.7% G337G (GGT→GGG tap ← methyl‑accepting chemotaxis protein IV
RA 1,986,321 T→C 13.2% S296G (AGC→GGC)  Y75_RS10005 ← arabinose ABC transporter ATP binding protein AraG
RA 1,989,132 A→C 13.4% Y24S (TAC→TCC)  Y75_RS10015 → non‑heme ferritin‑like protein
RA 2,016,339 T→C 11.2% I325T (ATC→ACC)  fliF → flagellar basal body M‑ring protein FliF
RA 2,072,206 G→A 11.6% Q53* (CAA→TAA)  Y75_RS10480 ← IS3‑like element IS2 family transposase
RA 2,072,207 C→T 11.5% R52R (CGG→CGA Y75_RS10480 ← IS3‑like element IS2 family transposase
RA 2,152,044 A→C 18.9% V360G (GTG→GGG)  yegI ← protein kinase YegI
RA 2,173,679 A→C 12.6% pseudogene (192/306 nt) Y75_RS10955 ← DeoR/GlpR transcriptional regulator
MC JC 2,176,983 Δ1,199 bp 100% Y75_RS10980[gatA] Y75_RS10980, [gatA]
RA 2,277,683 T→G 11.7% G57G (GGT→GGG Y75_RS11430 → microcin C ABC transporter permease YejB
RA 2,307,856 G→T 5.8% intergenic (+129/+586) eco → / ← mqo serine protease inhibitor ecotin/malate dehydrogenase (quinone)
RA 2,307,857 G→C 7.5% intergenic (+130/+585) eco → / ← mqo serine protease inhibitor ecotin/malate dehydrogenase (quinone)
RA 2,307,871 A→G 8.8% intergenic (+144/+571) eco → / ← mqo serine protease inhibitor ecotin/malate dehydrogenase (quinone)
RA 2,308,747 T→G 7.9% T448P (ACC→CCC)  mqo ← malate dehydrogenase (quinone)
RA 2,312,466 C→T 5.5% T70T (ACG→ACA alkB ← DNA oxidative demethylase AlkB
MC JC 2,320,795 Δ1,336 bp 100% Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,360,200 A→C 10.1% T4P (ACC→CCC)  glpC → anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
RA 2,464,671 T→C 14.2% G415G (GGA→GGG fadI ← acetyl‑CoA C‑acyltransferase FadI
RA 2,469,925 T→C 11.3% T177A (ACC→GCC)  mlaA ← phospholipid‑binding lipoprotein MlaA
RA 2,573,524 T→G 14.1% T46P (ACC→CCC)  eutQ ← ethanolamine utilization acetate kinase EutQ
RA 2,576,109 T→G 10.8% T309P (ACC→CCC)  maeB ← NADP‑dependent oxaloacetate‑decarboxylating malate dehydrogenase
RA 2,606,884 C→G 14.8% R438G (CGG→GGG)  hyfF → hydrogenase 4 subunit F
RA 2,660,496 A→T 100% C98S (TGC→AGC)  iscR ← Fe‑S cluster assembly transcriptional regulator IscR
RA 2,669,181 A→C 8.8% T46P (ACC→CCC)  Y75_RS13285 → 3‑phenylpropionate/cinnamic acid dioxygenase subunit beta
RA 2,825,084 T→G 12.8% W13G (TGG→GGG)  srlE → PTS glucitol/sorbitol transporter subunit IIB
RA 2,827,162 T→G 16.0% V257G (GTG→GGG)  srlD → sorbitol‑6‑phosphate dehydrogenase
RA 2,827,233 T→C 9.3% intergenic (+61/‑44) srlD → / → gutM sorbitol‑6‑phosphate dehydrogenase/transcriptional regulator GutM
RA 2,864,722 T→G 12.0% G322G (GGT→GGG Y75_RS14290 → GntP family transporter
RA 2,866,109 C→A 100% pseudogene (99/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 2,875,251 G→T 12.3% L5F (TTG→TTT iap → alkaline phosphatase isozyme conversion aminopeptidase
RA 2,906,975 C→G 100% D450H (GAC→CAC)  pyrG ← CTP synthase (glutamine hydrolyzing)
MC JC 2,910,136 Δ12 bp 100% coding (2161‑2172/2235 nt) Y75_RS14515 ← GTP pyrophosphokinase
RA 2,939,008 A→C 13.9% C298G (TGC→GGC)  rlmM ← 23S rRNA (cytidine(2498)‑2'‑O)‑methyltransferase RlmM
RA 2,948,829 A→G 10.0% E311G (GAG→GGG)  argA → amino‑acid N‑acetyltransferase
RA 2,973,749 T→G 12.8% T308P (ACT→CCT)  aas ← bifunctional acyl‑ACP‑‑phospholipid O‑acyltransferase/long‑chain‑fatty‑acid‑‑ACP ligase
RA 2,973,763 A→T 7.6% I303N (ATC→AAC)  aas ← bifunctional acyl‑ACP‑‑phospholipid O‑acyltransferase/long‑chain‑fatty‑acid‑‑ACP ligase
RA 2,976,798 T→C 11.5% E253G (GAG→GGG)  lysA ← diaminopimelate decarboxylase
RA 2,976,802 G→C 11.7% R252G (CGT→GGT)  lysA ← diaminopimelate decarboxylase
RA 3,089,466 T→G 12.1% V155G (GTC→GGC)  endA → deoxyribonuclease I
RA 3,137,862 T→G 7.7% G160G (GGT→GGG yghU → glutathione‑dependent disulfide‑bond oxidoreductase
RA 3,198,591 A→G 11.2% I344T (ATC→ACC)  Y75_RS15900 ← inorganic triphosphatase
RA 3,205,094 G→A 25.2% intergenic (‑182/‑25) ttdR ← / → ttdA DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
RA 3,205,132 G→A 14.6% S5N (AGT→AAT) ‡ ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,133 T→A 13.6% S5R (AGT→AGA) ‡ ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,139 G→A 24.0% K7K (AAG→AAA ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,140 C→A 12.6% Q8K (CAA→AAA)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,143 C→A 11.5% Q9K (CAG→AAG)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,162 C→A 8.9% T15K (ACA→AAA)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,236,547 A→G 13.8% E194G (GAG→GGG)  Y75_RS16075 → Gfo/Idh/MocA family oxidoreductase
MC JC 3,261,134 Δ1,199 bp 100% Y75_RS16205[tdcD] Y75_RS16205, [tdcD]
RA 3,308,855 G→C 15.3% intergenic (‑76/+33) nlpI ← / ← pnp lipoprotein NlpI/polyribonucleotide nucleotidyltransferase
JC 3,315,267 (CGACGTGCTTCTTCAGCAACG)1→2 100% coding (603/2673 nt) infB ← translation initiation factor IF‑2
RA 3,315,356 C→T 8.7% E172K (GAA→AAA)  infB ← translation initiation factor IF‑2
RA 3,315,372 A→T 11.0% T166T (ACT→ACA infB ← translation initiation factor IF‑2
RA 3,315,410 C→T 6.0% E154K (GAA→AAA)  infB ← translation initiation factor IF‑2
RA 3,370,730 A→C 10.8% G54G (GGT→GGG Y75_RS16755 ← N‑acetylmannosamine‑6‑phosphate 2‑epimerase
RA 3,375,420 C→A 12.1% Q233K (CAA→AAA)  dcuC → anaerobic C4‑dicarboxylate transporter DcuC
RA 3,411,477 C→T 5.8% intergenic (+55/‑31) fis → / → yhdJ DNA‑binding transcriptional regulator Fis/adenine‑specific DNA‑methyltransferase
RA 3,414,977 G→T 7.5% L30F (TTG→TTT acrF → multidrug efflux RND transporter permease subunit AcrF
RA 3,477,968 A→C 6.8% V75G (GTG→GGG)  oxyR ← DNA‑binding transcriptional regulator OxyR
RA 3,485,352 A→C 21.6% A590A (GCA→GCC ppc → phosphoenolpyruvate carboxylase
RA 3,485,358 T→C 18.5% A592A (GCT→GCC ppc → phosphoenolpyruvate carboxylase
RA 3,491,593 C→G 12.3% R365P (CGC→CCC)  Y75_RS17340 ← formate C‑acetyltransferase
RA 3,538,290 T→G 23.1% Y219S (TAC→TCC)  rhaS ← HTH‑type transcriptional activator RhaS
RA 3,571,090 C→G 8.7% A59G (GCG→GGG)  Y75_RS17735 → MFS transporter
RA 3,609,535 G→C 11.9% A207G (GCG→GGG)  fre ← NAD(P)H‑flavin reductase
RA 3,609,538 A→C 17.1% M206R (ATG→AGG)  fre ← NAD(P)H‑flavin reductase
RA 3,645,110 A→T 100% L140Q (CTG→CAG)  cyaA ← class I adenylate cyclase
RA 3,656,455 C→T 100% V91I (GTT→ATT)  rffM ← lipopolysaccharide N‑acetylmannosaminouronosyltransferase
RA 3,661,414 T→C 8.7% E41G (GAG→GGG)  rffA ← dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
JC 3,670,427 Δ27 bp 5.8% [rhoL] [rhoL]
RA 3,685,148 A→T 92.4% pseudogene (974/1645 nt) Y75_RS18285 ← acetolactate synthase 2 catalytic subunit
RA 3,732,053 A→C 15.2% T324P (ACC→CCC)  bglF → PTS beta‑glucoside transporter subunit IIABC
MC JC 3,746,911 Δ1,199 bp 100% Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,750,591 Δ1,199 bp 100% Y75_RS18600 Y75_RS18600
RA 3,764,880 G→T 7.9% T33N (ACC→AAC)  Y75_RS18670 ← hypothetical protein
RA 3,777,742 C→T 16.0% L311L (CTG→TTG)  Y75_RS18735 → PTS transporter subunit EIIC
RA 3,777,745 A→T 21.4% I312F (ATT→TTT)  Y75_RS18735 → PTS transporter subunit EIIC
RA 3,777,754 A→C 31.4% T315P (ACC→CCC)  Y75_RS18735 → PTS transporter subunit EIIC
RA 3,787,552 A→C 10.6% intergenic (+27/‑79) ivbL → / → ilvB ilvB operon leader peptide IvbL/acetolactate synthase large subunit
RA 3,856,941 T→C 5.1% L63P (CTC→CCC)  gpsA → NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
RA 3,857,507 A→C 19.5% T252P (ACC→CCC)  gpsA → NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
RA 3,857,513 A→C 21.2% T254P (ACC→CCC)  gpsA → NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
RA 3,890,038 A→G 10.2% S98P (TCG→CCG)  Y75_RS19280 ← L‑ribulose‑5‑phosphate 4‑epimerase
RA 3,890,726 T→C 12.7% E153G (GAG→GGG)  Y75_RS19285 ← L‑ribulose‑5‑phosphate 3‑epimerase
RA 3,942,186 G→T 8.3% Q6K (CAA→AAA)  bcsG ← cellulose biosynthesis protein BcsG
RA 3,942,675 A→C 17.9% V428G (GTG→GGG)  bcsE ← cellulose biosynthesis protein BcsE
RA 3,953,876 A→G 7.3% S879G (AGC→GGC)  bcsC → cellulose biosynthesis protein BcsC
RA 3,994,338 A→G 10.7% D231D (GAT→GAC Y75_RS19765 ← 23S rRNA (adenine(2030)‑N(6))‑methyltransferase
RA 4,012,909 A→C 9.4% H678P (CAC→CCC)  rbbA → ribosome‑associated ATPase/putative transporter RbbA
RA 4,017,888 A→C 12.1% S1112R (AGT→AGG Y75_RS19860 ← rhs element protein RhsB
RA 4,021,098 T→C 17.2% G42G (GGA→GGG Y75_RS19860 ← rhs element protein RhsB
RA 4,044,279 A→G 6.6% S90G (AGT→GGT)  livH → high‑affinity branched‑chain amino acid ABC transporter permease LivH
RA 4,071,644 C→A 10.9% N186K (AAC→AAA glgC → glucose‑1‑phosphate adenylyltransferase
RA 4,087,131 A→C 9.2% G67G (GGT→GGG malT ← HTH‑type transcriptional regulator MalT
RA 4,091,484 C→G 9.6% A380G (GCG→GGG)  malQ → 4‑alpha‑glucanotransferase
RA 4,091,507 T→G 15.4% C388G (TGT→GGT)  malQ → 4‑alpha‑glucanotransferase
RA 4,091,582 T→G 10.2% W413G (TGG→GGG)  malQ → 4‑alpha‑glucanotransferase
RA 4,104,271 T→C 12.0% L147P (CTC→CCC)  ompR → two‑component system response regulator OmpR
RA 4,104,281 C→G 10.3% R150R (CGC→CGG ompR → two‑component system response regulator OmpR
RA 4,108,967 T→G 8.5% S331A (TCG→GCG)  Y75_RS20260 → DUF4153 domain‑containing protein
RA 4,179,667 A→T 11.0% G311G (GGT→GGA gspF ← type II secretion system inner membrane protein GspF
RA 4,262,217 A→C 9.2% T296P (ACC→CCC)  dinF → MATE family efflux transporter DinF
RA 4,273,250 C→G 6.6% R83G (CGC→GGC)  Y75_RS21155 → acid phosphatase AphA
RA 4,290,120 T→A 11.3% Y281F (TAT→TTT)  acs ← acetate‑‑CoA ligase
RA 4,330,626 A→G 5.8% intergenic (‑207/+451) yjdN ← / ← Y75_RS21430 VOC family metalloprotein YjdN/phnA family protein
RA 4,341,559 T→A 11.4% I51F (ATC→TTC)  adiC ← arginine/agmatine antiporter
RA 4,346,346 A→G 5.5% intergenic (‑40/‑243) melR ← / → melA transcriptional regulator MelR/alpha‑galactosidase
RA 4,371,271 Δ2 bp 100% pseudogene (182‑183/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter
RA 4,395,445 T→G 14.7% T249P (ACC→CCC)  rsgA ← small ribosomal subunit biogenesis GTPase RsgA
RA 4,399,551 C→G 12.5% A269G (GCT→GGT)  nnr → bifunctional ADP‑dependent NAD(P)H‑hydrate dehydratase/NAD(P)H‑hydrate epimerase
RA 4,408,585 T→G 12.7% G202G (GGT→GGG hflC → protease modulator HflC
RA 4,421,891 G→A 5.1% S87N (AGT→AAT)  yjfP → esterase
RA 4,428,816 T→G 8.3% V146G (GTG→GGG)  Y75_RS21910 → L‑ribulose‑5‑phosphate 4‑epimerase UlaF
RA 4,479,393 A→G 11.0% E197G (GAG→GGG)  Y75_RS22170 → TetR/AcrR family transcriptional regulator
RA 4,527,463 T→G 11.5% T438P (ACC→CCC)  Y75_RS22420 ← xylonate dehydratase YjhG
RA 4,595,559 T→C 11.2% D134G (GAC→GGC)  btsT ← pyruvate/proton symporter BtsT
RA 4,611,582 A→G 20.2% S267P (TCC→CCC)  rsmC ← 16S rRNA (guanine(1207)‑N(2))‑methyltransferase RsmC
RA 4,623,416 A→C 14.1% T170P (ACC→CCC)  deoA → thymidine phosphorylase
RA 4,624,158 T→G 12.1% V417G (GTG→GGG)  deoA → thymidine phosphorylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1301041–1302370 1302407 38–1367 209 [0] [0] 166 Y75_RS06530–Y75_RS06530 Y75_RS06530,Y75_RS06530
* * ÷ NC_007779 1397907–1398957 1406267 7311–8361 65 [63] [0] 141 [Y75_RS07025]–[Y75_RS07065] [Y75_RS07025],Y75_RS07030,uspE,Y75_RS07040,ogt,abgT,Y75_RS07060,[Y75_RS07065]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 224412 =NA (NA)6 (0.030) 5/396 NT NA noncoding (642/1554 nt) Y75_RS01020 16S ribosomal RNA
?NC_007779 224936 = NA (NA)noncoding (1166/1554 nt) Y75_RS01020 16S ribosomal RNA
* ? NC_007779 273180 =NA (NA)198 (1.110) 116/408 NT 100% intergenic (+3/+145) Y75_RS01330/Y75_RS01335 hypothetical protein/IS5‑like element IS5 family transposase
?NC_007779 701988 = 0 (0.000)coding (29/1221 nt) nagC DNA‑binding transcriptional regulator NagC
* ? NC_007779 314625 =NA (NA)9 (0.050) 8/384 NT NA coding (111/300 nt) Y75_RS24740 transposase
?NC_007779 314851 = NA (NA)coding (41/867 nt) Y75_RS01555 IS3 family transposase
* ? NC_007779 374146 =154 (0.860)11 (0.060) 9/394 NT 5.9% intergenic (+41/‑537) dmpG/Y75_RS01815 4‑hydroxy‑2‑oxovalerate aldolase/3‑(3‑hydroxy‑phenyl)propionate transporter
?NC_007779 376553 = 204 (1.190)intergenic (+18/+206) Y75_RS01820/Y75_RS01825 DUF2058 domain‑containing protein/S‑formylglutathione hydrolase
* ? NC_007779 380758 =NA (NA)7 (0.040) 6/394 NT NA coding (229/361 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 381396 = NA (NA)coding (507/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 = 381377NA (NA)11 (0.060) 5/398 NT NA coding (488/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 = 381470 NA (NA)coding (581/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 571584 =246 (1.370)28 (0.160) 25/410 NT 10.2% intergenic (+110/‑7) Y75_RS02805/Y75_RS23245 helix‑turn‑helix transcriptional regulator/hypothetical protein
?NC_007779 = 1106952 NA (NA)intergenic (‑46/‑39) Y75_RS05455/Y75_RS05465 IS3‑like element IS2 family transposase/hypothetical protein
* ? NC_007779 = 571589246 (1.370)26 (0.150) 24/408 NT 9.6% intergenic (+115/‑2) Y75_RS02805/Y75_RS23245 helix‑turn‑helix transcriptional regulator/hypothetical protein
?NC_007779 = 1302368 NA (NA)intergenic (+10/‑528) Y75_RS06530/oppA IS3‑like element IS2 family transposase/oligopeptide ABC transporter substrate‑binding protein OppA
* ? NC_007779 607256 =NA (NA)13 (0.080) 8/358 NT 8.1% intergenic (+45/‑32) Y75_RS03010/Y75_RS03015 type I toxin‑antitoxin system toxin HokE/IS4‑like element IS421 family transposase
?NC_007779 608549 = 170 (0.950)intergenic (+149/+133) Y75_RS03015/entD IS4‑like element IS421 family transposase/enterobactin synthase subunit EntD
* ? NC_007779 = 689467NA (NA)124 (0.690) 73/410 NT 100% intergenic (‑32/+298) Y75_RS03405/lnt IS5‑like element IS5 family transposase/apolipoprotein N‑acyltransferase
?NC_007779 = 701992 0 (0.000)coding (25/1221 nt) nagC DNA‑binding transcriptional regulator NagC
* ? NC_007779 = 1051773216 (1.210)13 (0.070) 6/402 NT 5.9% intergenic (‑176/‑110) cspH/cspG cold shock‑like protein CspH/cold shock protein CspG
?NC_007779 = 1051897 203 (1.160)coding (15/213 nt) cspG cold shock protein CspG
* ? NC_007779 1209367 =74 (0.410)122 (0.740) 78/378 NT 63.7% coding (290/630 nt) Y75_RS06020 hypothetical protein
?NC_007779 1211196 = 71 (0.430)intergenic (‑6/‑66) Y75_RS06035/pinE tail fiber protein/site‑specific DNA recombinase
* ? NC_007779 = 120938271 (0.400)102 (0.620) 63/378 NT 59.9% coding (305/630 nt) Y75_RS06020 hypothetical protein
?NC_007779 = 1211179 71 (0.430)coding (12/495 nt) Y75_RS06035 tail fiber protein
* ? NC_007779 1294701 =95 (0.530)6 (0.030) 6/410 NT 5.9% intergenic (‑202/‑403) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 = 1398952 NA (NA)intergenic (+146/‑127) Y75_RS07025/Y75_RS07030 IS5‑like element IS5 family transposase/DUF2534 family protein
* ? NC_007779 = 129470486 (0.480)5 (0.030) 4/410 NT 5.5% intergenic (‑205/‑400) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 2176983 = NA (NA)intergenic (‑3/‑32) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
* ? NC_007779 1294773 =2 (0.010)262 (1.460) 121/410 NT 99.2% intergenic (‑274/‑331) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 = 1398952 NA (NA)intergenic (+146/‑127) Y75_RS07025/Y75_RS07030 IS5‑like element IS5 family transposase/DUF2534 family protein
* ? NC_007779 = 12947762 (0.010)199 (1.110) 105/410 NT 99.0% intergenic (‑277/‑328) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 1397758 = NA (NA)intergenic (‑122/‑32) ynaI/Y75_RS07025 low conductance mechanosensitive channel YnaI/IS5‑like element IS5 family transposase
* ? NC_007779 = 13010400 (0.000)163 (1.050)
+28 bp
82/354 NT 100% intergenic (+218/‑45) ychE/Y75_RS06530 NAAT family transporter YchE/IS3‑like element IS2 family transposase
?NC_007779 = 4311564 NA (NA)pseudogene (914/921 nt) alsK allose kinase
* ? NC_007779 1302408 =0 (0.000)159 (0.890) 89/410 NT 100% intergenic (+50/‑488) Y75_RS06530/oppA IS3‑like element IS2 family transposase/oligopeptide ABC transporter substrate‑binding protein OppA
?NC_007779 1397758 = NA (NA)intergenic (‑122/‑32) ynaI/Y75_RS07025 low conductance mechanosensitive channel YnaI/IS5‑like element IS5 family transposase
* ? NC_007779 = 1388812174 (0.970)19 (0.110) 10/398 NT 10.1% coding (379/1542 nt) tyrR transcriptional regulator TyrR
?NC_007779 = 1388962 171 (0.980)coding (529/1542 nt) tyrR transcriptional regulator TyrR
* ? NC_007779 1406268 =0 (0.000)138 (0.770) 83/410 NT 100% coding (12/1311 nt) Y75_RS07065 p‑aminobenzoyl‑glutamate hydrolase subunit AbgA
?NC_007779 1980209 = NA (NA)intergenic (‑298/+146) flhD/Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
* ? NC_007779 1463803 =200 (1.120)12 (0.070) 10/386 NT 5.8% coding (1197/1206 nt) Y75_RS07380 3‑oxoadipyl‑CoA thiolase
?NC_007779 1463937 = 202 (1.200)coding (99/1314 nt) paaF phenylacetate‑‑CoA ligase
* ? NC_007779 = 1817916174 (0.970)12 (0.070) 6/382 NT 6.4% intergenic (+74/+184) katE/chbG catalase HPII/chitin disaccharide deacetylase
?NC_007779 = 1818069 187 (1.120)intergenic (+227/+31) katE/chbG catalase HPII/chitin disaccharide deacetylase
* ? NC_007779 = 183039591 (0.510)7 (0.040) 7/392 NT 7.4% coding (1054/1479 nt) astD succinylglutamate‑semialdehyde dehydrogenase
?NC_007779 = 1830409 87 (0.510)coding (1040/1479 nt) astD succinylglutamate‑semialdehyde dehydrogenase
* ? NC_007779 1881541 =0 (0.000)5 (0.030) 5/406 NT 100% coding (122/360 nt) yeaR DUF1971 domain‑containing protein
?NC_007779 2519722 = NA (NA)intergenic (+32/‑55) nupC/Y75_RS12570 nucleoside permease NupC/IS4‑like element IS421 family transposase
* ? NC_007779 1881541 =0 (0.000)180 (1.010) 106/406 NT 100% coding (122/360 nt) yeaR DUF1971 domain‑containing protein
?NC_007779 = 2521060 NA (NA)intergenic (+171/+29) Y75_RS12570/Y75_RS12575 IS4‑like element IS421 family transposase/sensor domain‑containing phosphodiesterase
* ? NC_007779 = 18815519 (0.050)186 (1.050) 94/404 NT 95.4% coding (112/360 nt) yeaR DUF1971 domain‑containing protein
?NC_007779 2519723 = NA (NA)intergenic (+33/‑54) nupC/Y75_RS12570 nucleoside permease NupC/IS4‑like element IS421 family transposase
* ? NC_007779 = 18815519 (0.050)6 (0.030) 5/404 NT 40.4% coding (112/360 nt) yeaR DUF1971 domain‑containing protein
?NC_007779 = 2521059 NA (NA)intergenic (+170/+30) Y75_RS12570/Y75_RS12575 IS4‑like element IS421 family transposase/sensor domain‑containing phosphodiesterase
* ? NC_007779 2071829 =NA (NA)33 (0.190) 7/388 NT 83.3% coding (174/914 nt) Y75_RS10480 IS3‑like element IS2 family transposase
?NC_007779 2072195 = 7 (0.040)coding (168/361 nt) Y75_RS10480 IS3‑like element IS2 family transposase
* ? NC_007779 = 2537411193 (1.080)15 (0.090) 6/390 NT 7.0% coding (503/762 nt) cysZ sulfate transporter CysZ
?NC_007779 = 2537613 218 (1.280)coding (705/762 nt) cysZ sulfate transporter CysZ
* ? NC_007779 = 2712485177 (0.990)12 (0.070) 12/388 NT 6.9% coding (934/1335 nt) srmB ATP‑dependent RNA helicase SrmB
?NC_007779 = 2712564 158 (0.930)coding (1013/1335 nt) srmB ATP‑dependent RNA helicase SrmB
* ? NC_007779 = 2899085166 (0.930)10 (0.060) 7/396 NT 6.8% intergenic (‑156/‑163) Y75_RS14460/Y75_RS14465 SDR family oxidoreductase/MFS transporter
?NC_007779 = 2899239 112 (0.650)intergenic (‑310/‑9) Y75_RS14460/Y75_RS14465 SDR family oxidoreductase/MFS transporter
* ? NC_007779 = 37429350 (0.000)173 (1.010) 85/394 NT 100% intergenic (‑135/+9) Y75_RS18555/Y75_RS18560 4'‑phosphopantetheinyl transferase/IS3‑like element IS2 family transposase
?NC_007779 3744272 = 0 (0.000)intergenic (‑55/+21) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
* ? NC_007779 3748316 =0 (0.000)188 (1.050) 97/410 NT 100% pseudogene (665/873 nt) tnaB low affinity tryptophan permease TnaB
?NC_007779 = 4056987 NA (NA)coding (391/417 nt) Y75_RS20060 hypothetical protein
* ? NC_007779 = 37483244 (0.020)195 (1.090) 95/410 NT 98.0% pseudogene (657/873 nt) tnaB low affinity tryptophan permease TnaB
?NC_007779 4056220 = NA (NA)intergenic (‑209/+15) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
* ? NC_007779 3876427 =NA (NA)3 (0.020) 3/388 NT 9.9% coding (1806/4134 nt) Y75_RS19225 RHS element protein RhsA
?NC_007779 3876545 = 29 (0.160)coding (1688/4134 nt) Y75_RS19225 RHS element protein RhsA
* ? NC_007779 3970002 =174 (0.970)10 (0.060) 7/398 NT 5.5% coding (822/1650 nt) treF alpha,alpha‑trehalase TreF
?NC_007779 3970183 = 176 (1.010)coding (641/1650 nt) treF alpha,alpha‑trehalase TreF
* ? NC_007779 4056221 =NA (NA)165 (0.930) 87/408 NT 97.6% intergenic (‑210/+14) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
?NC_007779 4370787 = 4 (0.020)pseudogene (667/1304 nt) Y75_RS21600 anaerobic C4‑dicarboxylate transporter
* ? NC_007779 = 4056987NA (NA)210 (1.170) 108/410 NT 97.7% coding (391/417 nt) Y75_RS20060 hypothetical protein
?NC_007779 = 4370795 5 (0.030)pseudogene (659/1304 nt) Y75_RS21600 anaerobic C4‑dicarboxylate transporter
* ? NC_007779 = 43103621 (0.010)181 (1.030) 85/402 NT 99.7% intergenic (‑286/‑33) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 4311562 = 0 (0.000)pseudogene (916/921 nt) alsK allose kinase